The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Marcelo L Larramendy - One of the best experts on this subject based on the ideXlab platform.
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Carbamates: A study on genotoxic, cytotoxic, and apoptotic effects induced in Chinese hamster ovary (CHO-K1) cells.
Toxicology in vitro : an international journal published in association with BIBRA, 2015Co-Authors: Sonia Soloneski, Maciej Kujawski, Anna Scuto, Marcelo L LarramendyAbstract:In vitro effects of the carbamates pirimicarb and Zineb and their formulations Aficida® (50% pirimicarb) and Azzurro® (70% Zineb), respectively, were evaluated in Chinese hamster ovary (CHO-K1) cells. Whereas the cytokinesis-blocked micronucleus cytome assay was employed to test for genotoxicity, MTT, neutral red (NR), and apoptosis evaluation were used as tests for estimating cell viability and succinic dehydrogenase activity, respectively. Concentrations tested were 10-300 μg/ml for pirimicarb and Aficida®, and 1-50 μg/ml for Zineb and Azzurro®. All compounds were able to increase the frequency of micronuclei. A marked reduction in the nuclear division index was observed after treatment with 5 μg/ml of Zineb and Azzurro® and 10 μg/ml of Azzurro®. Alterations in the cellular morphology not allowing the recognition of binucleated cells exposed to 300 μg/ml pirimicarb and Aficida® as well as 10-50 μg/ml Zineb and Azzurro®. All four compounds induced inhibition of both cell viability and succinic dehydrogenase activity and trigger apoptosis in CHO-K1 cells, at least when exposed for 24 h. The data herein demonstrate the genotoxic and cytotoxic effects exerted by these carbamates and reveal the potential risk factor of these pesticides, still extensively used worldwide, for both human health and the environment.
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cytogenetic and microtubule array effects of the Zineb containing commercial fungicide formulation azzurro on meristematic root cells of allium cepa l
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2012Co-Authors: Nancy B Andrioli, Sonia Soloneski, Marcelo L Larramendy, Marta D MudryAbstract:Abstract Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the Zineb-containing commercial formulation Azzurro® (70% Zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro® (1 and 10 μg/ml) induced a significant increase in the frequency of CAs (P
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Cytogenetic and microtubule array effects of the Zineb-containing commercial fungicide formulation Azzurro(®) on meristematic root cells of Allium cepa L.
Mutation research, 2011Co-Authors: Nancy B Andrioli, Sonia Soloneski, Marcelo L Larramendy, Marta D MudryAbstract:Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the Zineb-containing commercial formulation Azzurro(®) (70% Zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro(®) (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P
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Vitamin E prevents ethylene bis(dithiocarbamate) pesticide Zineb-induced sister chromatid exchange in Chinese hamster ovary cells
Mutagenesis, 2003Co-Authors: Sonia Soloneski, Miguel A Reigosa, Marcelo L LarramendyAbstract:The in vitro effect of the antioxidant alpha-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide Zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both Zineb and azzurro activities were tested within the range 0.1-100.0 microg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 microg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in Zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 microg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 microg/ml Zineb or 1.0-25.0 microg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 microg/ml Zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of Zineb (0.1-10.0 microg/ml concentration range) and azzurro (0.1-25.0 microg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 microg/ml Zineb or 1.0-25.0 microg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with Zineb and azzurro.
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vitamin e prevents ethylene bis dithiocarbamate pesticide Zineb induced sister chromatid exchange in chinese hamster ovary cells
Mutagenesis, 2003Co-Authors: Sonia Soloneski, Miguel A Reigosa, Marcelo L LarramendyAbstract:Laboratorio de Citogenetica, Catedra de Citologia, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide Zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both Zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in Zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml Zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml Zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of Zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml Zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with Zineb and azzurro.
A. L. J. Rao - One of the best experts on this subject based on the ideXlab platform.
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Development of a derivative spectrophotometric method for the determination of fungicide zinc ethylenebisdithiocarbamate using sodium molybdate
Journal of the Brazilian Chemical Society, 2009Co-Authors: Manpreet Kaur, Varinder Kaur, Ashok Kumar Malik, Neelam Verma, Baldev Singh, A. L. J. RaoAbstract:Neste trabalho foi desenvolvido um procedimento espectrofotometrico derivativo para a determinacao de etilenobisditiocarbamato de zinco(II), Zineb, com base na formacao do complexo azul de molibdato de sodio em meio acido. A lei de Beer e obedecida ate 40 µg mL-1 de Zineb no comprimento de onda de 956 nm. O limite de deteccao foi de 0,006 µg mL-1 de Zineb para a razao S/N = 3 considerando diversos parâmetros como o efeito da concentracao de acido. A interferencia de diversos ions na determinacao de Zineb foi avaliada. A maioria dos metais alcalinos e sais metalicos nao causou interferencias. O procedimento tem boa sensibilidade e foi empregado para a determinacao de Zineb em amostras comerciais de alimentos contendo Dithane Z 78 e Hexathane 75% W.P.. Os resultados foram comparados com procedimentos previamente propostos. Zineb foi determinado com sucesso sem a ocorrencia de interferencias na presenca de outros ditiocarbamatos, tais como Ziram, Thiram, Ferbam etc.
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Development of new adsorbent chitin for column preconcentration and spectrophotometric trace determination of Ziram and Zineb in synthetic, commercial samples and food-stuffs.
Talanta, 2005Co-Authors: S. K. Mehta, Ashok Kumar Malik, Baldev Singh, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (Ziram) or zinc(II) ethylenebisdithiocarbamate (Zineb) present in a large volume of aqueous solution after preconcentration on a column using chitin-1-(2'pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram/Zineb are quantitatively retained on the column as Zn-PAN complex in the pH range 9.0-11.0 and at a flow rate of 1-8 ml/min. Complex adsorbed on chitin was eluted from the column with dimethylformamide (DMF) and absorbance of the eluate was measured at 550 nm against a reagent blank. Beer's law is obeyed over the concentration range 5.3-55.8 microg of Ziram and 6.8-49.0 microg of Zineb in 25 ml of the final DMF solution. Ten replicate determinations on a sample solution containing 45.22 microg of Ziram or 40.86 microg of Zineb gave a mean absorbance of 0.30 with a relative standard deviation 1.6 and 1.8%, respectively. The interference of various ions has been studied. Many alkali metals and metal salts do not interfere. The method has been employed to the determination of Ziram and Zineb in commercial samples and in various foodstuffs and the results were compared with the earlier reported methods.
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Column preconcentration and spectrophotometric determination of ziram and Zineb in commercial samples and foodstuffs using (1,2'-pyridylazo)-2-naphthol (PAN)-naphthalene as adsorbate.
Journal of agricultural and food chemistry, 2004Co-Authors: Ashok Kumar Malik, Vanita Sharma, Vaneet K Sharma, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (ziram) and zinc(II) ethylenebis(dithiocarbamate) (Zineb) after preconcentration on a column using naphthalene-(1,2'-pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram and Zineb are quantitatively retained on the column in the pH range of 9.0-12.5 and at a flow rate of 1-2 mL/min. The solid mass consisting of the Zn-PAN complex along with naphthalene is dissolved from the column with 5 mL of dimethylformamide (DMF). Absorbance of the complex was measured at 550 nm; Beer's law is obeyed over the concentration ranges of 2.0-22.0 microg of ziram and 5.0-19.8 microg of Zineb in 10 mL of the final DMF solution. Ten replicate determinations on a sample solution containing 20 microg of ziram and 18 microg of Zineb gave a mean absorbance of 0.33 with relative standard deviations of 0.80 and 0.70%, respectively. The interference of various ions has been studied. The method has been employed for the determination of ziram and Zineb in commercial samples and in various foodstuffs, and the results were compared with the earlier reported methods.
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column preconcentration and spectrophotometric determination of ziram and Zineb in commercial samples and foodstuffs using 1 2 pyridylazo 2 naphthol pan naphthalene as adsorbate
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Ashok Kumar Malik, Vanita Sharma, Vaneet K Sharma, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (ziram) and zinc(II) ethylenebis(dithiocarbamate) (Zineb) after preconcentration on a column using naphthalene-(1,2‘-pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram and Zineb are quantitatively retained on the column in the pH range of 9.0−12.5 and at a flow rate of 1−2 mL/min. The solid mass consisting of the Zn−PAN complex along with naphthalene is dissolved from the column with 5 mL of dimethylformamide (DMF). Absorbance of the complex was measured at 550 nm; Beer's law is obeyed over the concentration ranges of 2.0−22.0 μg of ziram and 5.0−19.8 μg of Zineb in 10 mL of the final DMF solution. Ten replicate determinations on a sample solution containing 20 μg of ziram and 18 μg of Zineb gave a mean absorbance of 0.33 with relative standard deviations of 0.80 and 0.70%, respectively. The interference of various ions has been studied. The method has been employed for the determination of ziram and Zineb in comme...
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Spectrophotometric method for the determination of ziram and Zineb with 1-(2-thiazolylazo)-2-naphthol (TAN) using Triton X-100 as the solubilizing agent
Journal of the Indian Chemical Society, 2003Co-Authors: S. K. Mehta, U. Gupta, A. L. J. RaoAbstract:A new, rapid, sensitive and selective method has been developed for the determination of ziram and Zineb using 1-(2-thiazolylazo)-2-naphthol (TAN) as the complexing reagent in the pH range of 8.0-8.5 in the presence of octylphenolpoly(ethylene glycol) ether i.e. Triton X-100. The violet complex shows λ m a x at 580 nm. The molar absorptivity is 7.52 x 10 4 dm 3 mol - 1 cm - 1 for ziram and Zineb and Sandell's sensitivity 0.0040 and 0.0036 μg cm - 2 for ziram and Zineb, respectively. The method has been used for direct determination of ziram and Zineb in synthetic mixtures, commercial formulations and crops residue.
Sonia Soloneski - One of the best experts on this subject based on the ideXlab platform.
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Carbamates: A study on genotoxic, cytotoxic, and apoptotic effects induced in Chinese hamster ovary (CHO-K1) cells.
Toxicology in vitro : an international journal published in association with BIBRA, 2015Co-Authors: Sonia Soloneski, Maciej Kujawski, Anna Scuto, Marcelo L LarramendyAbstract:In vitro effects of the carbamates pirimicarb and Zineb and their formulations Aficida® (50% pirimicarb) and Azzurro® (70% Zineb), respectively, were evaluated in Chinese hamster ovary (CHO-K1) cells. Whereas the cytokinesis-blocked micronucleus cytome assay was employed to test for genotoxicity, MTT, neutral red (NR), and apoptosis evaluation were used as tests for estimating cell viability and succinic dehydrogenase activity, respectively. Concentrations tested were 10-300 μg/ml for pirimicarb and Aficida®, and 1-50 μg/ml for Zineb and Azzurro®. All compounds were able to increase the frequency of micronuclei. A marked reduction in the nuclear division index was observed after treatment with 5 μg/ml of Zineb and Azzurro® and 10 μg/ml of Azzurro®. Alterations in the cellular morphology not allowing the recognition of binucleated cells exposed to 300 μg/ml pirimicarb and Aficida® as well as 10-50 μg/ml Zineb and Azzurro®. All four compounds induced inhibition of both cell viability and succinic dehydrogenase activity and trigger apoptosis in CHO-K1 cells, at least when exposed for 24 h. The data herein demonstrate the genotoxic and cytotoxic effects exerted by these carbamates and reveal the potential risk factor of these pesticides, still extensively used worldwide, for both human health and the environment.
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cytogenetic and microtubule array effects of the Zineb containing commercial fungicide formulation azzurro on meristematic root cells of allium cepa l
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2012Co-Authors: Nancy B Andrioli, Sonia Soloneski, Marcelo L Larramendy, Marta D MudryAbstract:Abstract Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the Zineb-containing commercial formulation Azzurro® (70% Zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro® (1 and 10 μg/ml) induced a significant increase in the frequency of CAs (P
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Cytogenetic and microtubule array effects of the Zineb-containing commercial fungicide formulation Azzurro(®) on meristematic root cells of Allium cepa L.
Mutation research, 2011Co-Authors: Nancy B Andrioli, Sonia Soloneski, Marcelo L Larramendy, Marta D MudryAbstract:Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the Zineb-containing commercial formulation Azzurro(®) (70% Zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro(®) (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P
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Vitamin E prevents ethylene bis(dithiocarbamate) pesticide Zineb-induced sister chromatid exchange in Chinese hamster ovary cells
Mutagenesis, 2003Co-Authors: Sonia Soloneski, Miguel A Reigosa, Marcelo L LarramendyAbstract:The in vitro effect of the antioxidant alpha-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide Zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both Zineb and azzurro activities were tested within the range 0.1-100.0 microg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 microg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in Zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 microg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 microg/ml Zineb or 1.0-25.0 microg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 microg/ml Zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of Zineb (0.1-10.0 microg/ml concentration range) and azzurro (0.1-25.0 microg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 microg/ml Zineb or 1.0-25.0 microg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with Zineb and azzurro.
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vitamin e prevents ethylene bis dithiocarbamate pesticide Zineb induced sister chromatid exchange in chinese hamster ovary cells
Mutagenesis, 2003Co-Authors: Sonia Soloneski, Miguel A Reigosa, Marcelo L LarramendyAbstract:Laboratorio de Citogenetica, Catedra de Citologia, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide Zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both Zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in Zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml Zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml Zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of Zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml Zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with Zineb and azzurro.
Ashok Kumar Malik - One of the best experts on this subject based on the ideXlab platform.
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spectrophotometric determination of ziram and Zineb in commercial samples and food stuffs using par naphthalene as column adsorbate for preconcentration
2012Co-Authors: Shivender Singh Saini, Vanita Sharma, Ashok Kumar MalikAbstract:A procedure has been developed for the determination of zinc(II) bis (dimethyldithiocarbamate) (Ziram) and zinc(II) ethylenebisdithiocarbamate (Zineb) after preconcentration on a column using naphthalene-PAR as adsorbent. Ziram and Zineb are quantitatively retained on the column in the pH range 9.0- 12.5 and at a flow rate of 1-2 ml/min. The solid mass consisting of the Zn-PAR complex along with naphthalene is dissolved from the column with 5 ml of dimethylformamide (DMF). The absorbance is measured at 490 nm with a spectrophotometer against the reagent blank. Beer's law is obeyed over the concentration range 0.1- 15 µg of ziram and 0.1 -13 µg of Zineb in 5 ml of the final DMF solution. Ten replicate determinations on a sample solution containing 40 µg of ziram and 36 µg of Zineb gave a mean absorbance of 0.30 with a relative standard deviation 0.98%. The interference of various ions has been studied and the method has been employed to the determination of ziram and Zineb in commercial samples and in various foodstuffs.
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Development of a derivative spectrophotometric method for the determination of fungicide zinc ethylenebisdithiocarbamate using sodium molybdate
Journal of the Brazilian Chemical Society, 2009Co-Authors: Manpreet Kaur, Varinder Kaur, Ashok Kumar Malik, Neelam Verma, Baldev Singh, A. L. J. RaoAbstract:Neste trabalho foi desenvolvido um procedimento espectrofotometrico derivativo para a determinacao de etilenobisditiocarbamato de zinco(II), Zineb, com base na formacao do complexo azul de molibdato de sodio em meio acido. A lei de Beer e obedecida ate 40 µg mL-1 de Zineb no comprimento de onda de 956 nm. O limite de deteccao foi de 0,006 µg mL-1 de Zineb para a razao S/N = 3 considerando diversos parâmetros como o efeito da concentracao de acido. A interferencia de diversos ions na determinacao de Zineb foi avaliada. A maioria dos metais alcalinos e sais metalicos nao causou interferencias. O procedimento tem boa sensibilidade e foi empregado para a determinacao de Zineb em amostras comerciais de alimentos contendo Dithane Z 78 e Hexathane 75% W.P.. Os resultados foram comparados com procedimentos previamente propostos. Zineb foi determinado com sucesso sem a ocorrencia de interferencias na presenca de outros ditiocarbamatos, tais como Ziram, Thiram, Ferbam etc.
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Capillary Electrophoretic-Ultraviolet Method for the Separation and Estimation of Zineb, Maneb, and Ferbam in Food Samples
Journal of AOAC International, 2007Co-Authors: Jatinder Singh Aulakh, Ashok Kumar Malik, Agnes Fekete, Rakesh Kumar Mahajan, Philippe Schmitt-kopplinAbstract:A simple and sensitive capillary electrophoretic method with ultraviolet detection has been developed for the separation and determination of ferbam [iron(III)-dimethyldithiocarbamate], maneb [manganese(II)-ethylenebisdithiocarbamate] and Zineb [zinc(II)-ethylenebisdithiocarbamate], in borate buffer, after their acidic decomposition and complexation with CDTA (trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid monohydrate), as CDTA-metal complexes of Fe+3, Mn +2, and Zn+2. The determination is dependent on the pH and the nature of the buffer solutions. In this method, the detection limit (signal-to-noise ratio = 3) is 0.0013, 0.0022, and 0.0023 mM for ferbam, maneb, and Zineb, respectively. The relative standard deviation for the analysis of 1 mM of each was found to be 1.5 +/- 0.2%. The method was successfully applied for the analysis of red beans and grain samples spiked with ferbam, maneb, and Zineb. The applicability of capillary electrophoresis as a useful tool for the simultaneous determination and analysis of ferbam, maneb, and Zineb is demonstrated.
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Development of new adsorbent chitin for column preconcentration and spectrophotometric trace determination of Ziram and Zineb in synthetic, commercial samples and food-stuffs.
Talanta, 2005Co-Authors: S. K. Mehta, Ashok Kumar Malik, Baldev Singh, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (Ziram) or zinc(II) ethylenebisdithiocarbamate (Zineb) present in a large volume of aqueous solution after preconcentration on a column using chitin-1-(2'pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram/Zineb are quantitatively retained on the column as Zn-PAN complex in the pH range 9.0-11.0 and at a flow rate of 1-8 ml/min. Complex adsorbed on chitin was eluted from the column with dimethylformamide (DMF) and absorbance of the eluate was measured at 550 nm against a reagent blank. Beer's law is obeyed over the concentration range 5.3-55.8 microg of Ziram and 6.8-49.0 microg of Zineb in 25 ml of the final DMF solution. Ten replicate determinations on a sample solution containing 45.22 microg of Ziram or 40.86 microg of Zineb gave a mean absorbance of 0.30 with a relative standard deviation 1.6 and 1.8%, respectively. The interference of various ions has been studied. Many alkali metals and metal salts do not interfere. The method has been employed to the determination of Ziram and Zineb in commercial samples and in various foodstuffs and the results were compared with the earlier reported methods.
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column preconcentration and spectrophotometric trace determination of ziram and Zineb using chitin as an adsorbate
Indian journal of chemistry. Sect. A: Inorganic physical theoretical & analytical, 2005Co-Authors: S. K. Mehta, Ashok Kumar Malik, Baldev Singh, Usha Gupta, Lj A RaoAbstract:A new method has been developed for the determination of Ziram and Zineb present in a large volume of aqueous solution by complex formation on a natural polymer chitin loaded with 4-(2'-pyridylazo)resorcinol. Ziram/Zineb is quantitatively retained on the column and exchange the zinc present in them with PAR in the pH range 9.0-12.5 at a flow rate of 1-10 ml/min. The complex adsorbed on chitin is eluted from the column with 10 ml of dimethylformamide and absorbance of the eluate measured at 490 nm against a reagent blank. The molar absorptivity is found to be 9.2 x 10 4 1 mol - 1 cm - 1 . Sandell's sensitivity for Ziram and Zineb is found to be 0.0033 and 0.0029 μg cm 2 , respectively. The method can be applied for the determination of Ziram and Zineb in synthetic, commercial samples and in various foodstuffs.
Smita Agarwal - One of the best experts on this subject based on the ideXlab platform.
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Quantification of ziram and Zineb residues in fog-water samples.
Talanta, 2005Co-Authors: Smita Agarwal, Shankar G. Aggarwal, Pahup SinghAbstract:The present paper describes the extractive quantification of zinc-dithiocarbamate fungicides, i.e. ziram (zinc bis-dimethyldithiocarbamate) and Zineb (zinc ethylene-1,2-bis-dithiocarbamate) in fog-water samples. The method is based on the releasing of equivalent amount of zinc from the fungicides and its subsequent determination by visible spectrophotometry or by flame-atomic absorption spectrometry (flame-AAS). For spectrophotometry, the sample contained up to 48mug of ziram and 42mug of Zineb was first equilibrated with chloroform. The recovery results show that only ziram content was extracted into chloroform. Then, the sample was treated with NH(4)SCN and surfactants (i.e. CPC and TX-100) solutions, and extracted with toluene to remove interference of inorganic zinc and other metal ions, if present in the sample. The residue was further used for Zineb determination. The chloroform extract and residue were then digested separately with nitric acid to release Zn(II), which were then analyzed spectrophotometerically with 4-(2-pyridylazo)-resorcinol in the micellar medium (TX-100) for the determination of ziram and Zineb, respectively. The complex shows lambda(max) at 495nm. The molar absorptivity in terms of ziram/Zineb was determined to be (8.05) x 10(4)Lmole(-1)cm(-1). The detection limits for ziram and Zineb were calculated to be 20 and 21mugL(-1) (with R.S.D. < 1.5%), respectively. Whereas, the optimum concentration ranges were 0.08-1.6 and 0.07-1.4mgL(-1), respectively. Alternatively, the Zn contents present in chloroform extract and in residue were directly analyzed using flame-AAS without undergoing the digestion procedure, and ziram and Zineb were determined, respectively. The optimum concentration ranges were 0.9-4.8 and 0.8-4.3mgL(-1), while the detection limits were calculated to be 145 and 144mugL(-1), respectively with R.S.D. < 2.5%. The methods are free from interference of almost all ions [including Zn(II)] and other dithiocarbamate pesticides, which can commonly associate with ziram/Zineb in fog-water.
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quantification of ziram and Zineb residues in fog water samples
Talanta, 2004Co-Authors: Smita Agarwal, Shankar G. Aggarwal, Pahup SinghAbstract:Abstract The present paper describes the extractive quantification of zinc-dithiocarbamate fungicides, i.e. ziram (zinc bis-dimethyldithiocarbamate) and Zineb (zinc ethylene-1,2-bis-dithiocarbamate) in fog-water samples. The method is based on the releasing of equivalent amount of zinc from the fungicides and its subsequent determination by visible spectrophotometry or by flame-atomic absorption spectrometry (flame-AAS). For spectrophotometry, the sample contained up to 48 μg of ziram and 42 μg of Zineb was first equilibrated with chloroform. The recovery results show that only ziram content was extracted into chloroform. Then, the sample was treated with NH 4 SCN and surfactants (i.e. CPC and TX-100) solutions, and extracted with toluene to remove interference of inorganic zinc and other metal ions, if present in the sample. The residue was further used for Zineb determination. The chloroform extract and residue were then digested separately with nitric acid to release Zn(II), which were then analyzed spectrophotometerically with 4-(2-pyridylazo)-resorcinol in the micellar medium (TX-100) for the determination of ziram and Zineb, respectively. The complex shows λ max at 495 nm. The molar absorptivity in terms of ziram/Zineb was determined to be (8.05) × 10 4 L mole −1 cm −1 . The detection limits for ziram and Zineb were calculated to be 20 and 21 μg L −1 (with R.S.D. −1 , respectively. Alternatively, the Zn contents present in chloroform extract and in residue were directly analyzed using flame-AAS without undergoing the digestion procedure, and ziram and Zineb were determined, respectively. The optimum concentration ranges were 0.9–4.8 and 0.8–4.3 mg L −1 , while the detection limits were calculated to be 145 and 144 μg L −1 , respectively with R.S.D.
