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Tomoyuki Kawada - One of the best experts on this subject based on the ideXlab platform.
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www.mdpi.com/journal/ijerph Article
2015Co-Authors: Carbamate Pesticide-induced, Maiko Kobayashi, Apoptosis Human, T Lymphocytes, Tomoyuki KawadaAbstract:Abstract: We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or Ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, Ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram> Ziram> maneb> carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c
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Carbamate pesticide-induced apoptosis in human T lymphocytes.
International journal of environmental research and public health, 2015Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or Ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, Ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > Ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c.
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carbamate pesticide induced apoptosis and necrosis in human natural killer cells
Journal of Biological Regulators and Homeostatic Agents, 2014Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:Abstract We previously found that Ziram, a carbamate fungicide, significantly induced apoptosis and necrosis in human NK-92MI, a natural killer cell line. To investigate whether other carbamate pesticides also induce apoptosis and necrosis in human natural killer cell, we conducted further experiments with NK-92CI, a human natural killer cell line using a more sensitive assay. NK-92CI cells were treated with Ziram, thiram, maneb or carbaryl at 0.031-40 microM for 2-24 h in the present study. Apoptosis and necrosis were determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspases 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that Ziram and thiram also induced apoptosis and necrosis in a time- and dose-dependent manner; however, maneb and carbaryl induced apoptosis and necrosis only at higher doses in NK-92CI cells. The strength of the apoptosis-inducing effect differed among the pesticides, and the order was as follows: thiram > Ziram greater than maneb greater than carbaryl. NK-92CI was more sensitive to Ziram than NK-92MI. Moreover, Ziram and thiram significantly increased the intracellular level of active caspase 3 in NK-92CI and caspase inhibitor significantly inhibited the apoptosis. Ziram and thiram significantly caused mitochondrial cytochrome-c release in NK-92CI. These findings indicate that carbamate pesticides can induce apoptosis in natural killer cells, and the apoptosis is mediated by both the caspase-cascade and mitochondrial cytochrome-c pathways.
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mechanism of Ziram induced apoptosis in human natural killer cells
International Journal of Immunopathology and Pharmacology, 2012Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:We previously found that Ziram, a dithiocarbamate fungicide, significantly inhibited natural killer (NK) activity in a dose-dependent manner. To explore the mechanism of this inhibition, we investigated Ziram-induced apoptosis in human NK cells. Human NK-92MI cells were treated with Ziram at 0.0625-4 µM for 2-64 h. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspases 3, 3/7, 8, and 9 and pan-caspase and mitochondrial cytochrome-c release were determined by flow cytometry. Disruption to mitochondrial transmembrane potential was determined with a MitoLight? Apoptosis Detection Kit. It was found that Ziram induced apoptosis in a dose- and time-dependent manner in human NK cells. Ziram increased the intracellular levels of active caspases 3, 3/7, 8, and 9 and pan-caspase in a dose-dependent manner, and a caspase-3 inhibitor, Z-DEVD-FMK, and a general caspase inhibitor, Z-VAD-FMK, partially but significantly inhibited the apoptosis. Ziram also disrupted mitochondrial transmembrane potential and caused mitochondrial cytochrome-c release in a dose-dependent manner. These findings indicate that Ziram can induce apoptosis in human NK cells, and the apoptosis is at least mediated by both the caspase-cascade and the mitochondria/cytochrome-c pathways.
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mechanism of Ziram induced apoptosis in human t lymphocytes
Archives of Toxicology, 2012Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:Ziram as a dithiocarbamate fungicide is widely used throughout the world in agriculture. We previously found that Ziram significantly inhibited cytotoxic T lymphocyte activity in a dose-dependent manner. To explore the mechanism of this inhibition, we investigated Ziram-induced apoptosis in human T lymphocytes. Jurkat T cells were treated with Ziram at 0.031–1 μM for 2–24 h. Freshly isolated primary human T cells were treated with Ziram at 0.0625–1 μM for 15 and 24 h. Apoptosis was determined by FITC-Annexin V/PI staining and the TUNEL assay. To explore the mechanism of apoptosis, intracellular levels of active caspases 3, 3/7, 8, and 9 and pan-caspase and mitochondrial cytochrome-c release were determined by flow cytometry. Disruption to mitochondrial transmembrane potential was determined with a MitoLight™ Apoptosis Detection Kit. We found that Ziram induced apoptosis in a time- and dose-dependent manner in both Jurkat cells and primary human T cells. The primary human T cells were more sensitive to Ziram than the Jurkat cell line. Ziram induced increases in active caspases 3, 3/7, 8, and 9 and pan-caspase in a dose-dependent manner, and a caspase-3 inhibitor, Z-DEVD-FMK, partially but significantly inhibited the apoptosis. Moreover, a general caspase inhibitor, Z-VAD-FMK, significantly and almost completely blocked the apoptosis. Ziram also disrupted mitochondrial transmembrane potential and caused mitochondrial cytochrome-c release. These findings indicate that Ziram can induce apoptosis in human T cells, and the apoptosis is mediated by both the caspase-cascade and the mitochondria/cytochrome-c pathways.
Maiko Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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www.mdpi.com/journal/ijerph Article
2015Co-Authors: Carbamate Pesticide-induced, Maiko Kobayashi, Apoptosis Human, T Lymphocytes, Tomoyuki KawadaAbstract:Abstract: We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or Ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, Ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram> Ziram> maneb> carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c
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Carbamate pesticide-induced apoptosis in human T lymphocytes.
International journal of environmental research and public health, 2015Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or Ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, Ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > Ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c.
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carbamate pesticide induced apoptosis and necrosis in human natural killer cells
Journal of Biological Regulators and Homeostatic Agents, 2014Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:Abstract We previously found that Ziram, a carbamate fungicide, significantly induced apoptosis and necrosis in human NK-92MI, a natural killer cell line. To investigate whether other carbamate pesticides also induce apoptosis and necrosis in human natural killer cell, we conducted further experiments with NK-92CI, a human natural killer cell line using a more sensitive assay. NK-92CI cells were treated with Ziram, thiram, maneb or carbaryl at 0.031-40 microM for 2-24 h in the present study. Apoptosis and necrosis were determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspases 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that Ziram and thiram also induced apoptosis and necrosis in a time- and dose-dependent manner; however, maneb and carbaryl induced apoptosis and necrosis only at higher doses in NK-92CI cells. The strength of the apoptosis-inducing effect differed among the pesticides, and the order was as follows: thiram > Ziram greater than maneb greater than carbaryl. NK-92CI was more sensitive to Ziram than NK-92MI. Moreover, Ziram and thiram significantly increased the intracellular level of active caspase 3 in NK-92CI and caspase inhibitor significantly inhibited the apoptosis. Ziram and thiram significantly caused mitochondrial cytochrome-c release in NK-92CI. These findings indicate that carbamate pesticides can induce apoptosis in natural killer cells, and the apoptosis is mediated by both the caspase-cascade and mitochondrial cytochrome-c pathways.
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mechanism of Ziram induced apoptosis in human natural killer cells
International Journal of Immunopathology and Pharmacology, 2012Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:We previously found that Ziram, a dithiocarbamate fungicide, significantly inhibited natural killer (NK) activity in a dose-dependent manner. To explore the mechanism of this inhibition, we investigated Ziram-induced apoptosis in human NK cells. Human NK-92MI cells were treated with Ziram at 0.0625-4 µM for 2-64 h. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspases 3, 3/7, 8, and 9 and pan-caspase and mitochondrial cytochrome-c release were determined by flow cytometry. Disruption to mitochondrial transmembrane potential was determined with a MitoLight? Apoptosis Detection Kit. It was found that Ziram induced apoptosis in a dose- and time-dependent manner in human NK cells. Ziram increased the intracellular levels of active caspases 3, 3/7, 8, and 9 and pan-caspase in a dose-dependent manner, and a caspase-3 inhibitor, Z-DEVD-FMK, and a general caspase inhibitor, Z-VAD-FMK, partially but significantly inhibited the apoptosis. Ziram also disrupted mitochondrial transmembrane potential and caused mitochondrial cytochrome-c release in a dose-dependent manner. These findings indicate that Ziram can induce apoptosis in human NK cells, and the apoptosis is at least mediated by both the caspase-cascade and the mitochondria/cytochrome-c pathways.
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mechanism of Ziram induced apoptosis in human t lymphocytes
Archives of Toxicology, 2012Co-Authors: Maiko Kobayashi, Tomoyuki KawadaAbstract:Ziram as a dithiocarbamate fungicide is widely used throughout the world in agriculture. We previously found that Ziram significantly inhibited cytotoxic T lymphocyte activity in a dose-dependent manner. To explore the mechanism of this inhibition, we investigated Ziram-induced apoptosis in human T lymphocytes. Jurkat T cells were treated with Ziram at 0.031–1 μM for 2–24 h. Freshly isolated primary human T cells were treated with Ziram at 0.0625–1 μM for 15 and 24 h. Apoptosis was determined by FITC-Annexin V/PI staining and the TUNEL assay. To explore the mechanism of apoptosis, intracellular levels of active caspases 3, 3/7, 8, and 9 and pan-caspase and mitochondrial cytochrome-c release were determined by flow cytometry. Disruption to mitochondrial transmembrane potential was determined with a MitoLight™ Apoptosis Detection Kit. We found that Ziram induced apoptosis in a time- and dose-dependent manner in both Jurkat cells and primary human T cells. The primary human T cells were more sensitive to Ziram than the Jurkat cell line. Ziram induced increases in active caspases 3, 3/7, 8, and 9 and pan-caspase in a dose-dependent manner, and a caspase-3 inhibitor, Z-DEVD-FMK, partially but significantly inhibited the apoptosis. Moreover, a general caspase inhibitor, Z-VAD-FMK, significantly and almost completely blocked the apoptosis. Ziram also disrupted mitochondrial transmembrane potential and caused mitochondrial cytochrome-c release. These findings indicate that Ziram can induce apoptosis in human T cells, and the apoptosis is mediated by both the caspase-cascade and the mitochondria/cytochrome-c pathways.
A. L. J. Rao - One of the best experts on this subject based on the ideXlab platform.
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Development of new adsorbent chitin for column preconcentration and spectrophotometric trace determination of Ziram and Zineb in synthetic, commercial samples and food-stuffs.
Talanta, 2005Co-Authors: S. K. Mehta, Ashok Kumar Malik, Baldev Singh, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (Ziram) or zinc(II) ethylenebisdithiocarbamate (Zineb) present in a large volume of aqueous solution after preconcentration on a column using chitin-1-(2'pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram/Zineb are quantitatively retained on the column as Zn-PAN complex in the pH range 9.0-11.0 and at a flow rate of 1-8 ml/min. Complex adsorbed on chitin was eluted from the column with dimethylformamide (DMF) and absorbance of the eluate was measured at 550 nm against a reagent blank. Beer's law is obeyed over the concentration range 5.3-55.8 microg of Ziram and 6.8-49.0 microg of Zineb in 25 ml of the final DMF solution. Ten replicate determinations on a sample solution containing 45.22 microg of Ziram or 40.86 microg of Zineb gave a mean absorbance of 0.30 with a relative standard deviation 1.6 and 1.8%, respectively. The interference of various ions has been studied. Many alkali metals and metal salts do not interfere. The method has been employed to the determination of Ziram and Zineb in commercial samples and in various foodstuffs and the results were compared with the earlier reported methods.
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column preconcentration and spectrophotometric determination of Ziram and zineb in commercial samples and foodstuffs using 1 2 pyridylazo 2 naphthol pan naphthalene as adsorbate
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Ashok Kumar Malik, Vanita Sharma, Vaneet K Sharma, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (Ziram) and zinc(II) ethylenebis(dithiocarbamate) (zineb) after preconcentration on a column using naphthalene-(1,2‘-pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram and zineb are quantitatively retained on the column in the pH range of 9.0−12.5 and at a flow rate of 1−2 mL/min. The solid mass consisting of the Zn−PAN complex along with naphthalene is dissolved from the column with 5 mL of dimethylformamide (DMF). Absorbance of the complex was measured at 550 nm; Beer's law is obeyed over the concentration ranges of 2.0−22.0 μg of Ziram and 5.0−19.8 μg of zineb in 10 mL of the final DMF solution. Ten replicate determinations on a sample solution containing 20 μg of Ziram and 18 μg of zineb gave a mean absorbance of 0.33 with relative standard deviations of 0.80 and 0.70%, respectively. The interference of various ions has been studied. The method has been employed for the determination of Ziram and zineb in comme...
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Spectrophotometric method for the determination of Ziram and zineb with 1-(2-thiazolylazo)-2-naphthol (TAN) using Triton X-100 as the solubilizing agent
Journal of the Indian Chemical Society, 2003Co-Authors: S. K. Mehta, U. Gupta, A. L. J. RaoAbstract:A new, rapid, sensitive and selective method has been developed for the determination of Ziram and zineb using 1-(2-thiazolylazo)-2-naphthol (TAN) as the complexing reagent in the pH range of 8.0-8.5 in the presence of octylphenolpoly(ethylene glycol) ether i.e. Triton X-100. The violet complex shows λ m a x at 580 nm. The molar absorptivity is 7.52 x 10 4 dm 3 mol - 1 cm - 1 for Ziram and zineb and Sandell's sensitivity 0.0040 and 0.0036 μg cm - 2 for Ziram and zineb, respectively. The method has been used for direct determination of Ziram and zineb in synthetic mixtures, commercial formulations and crops residue.
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Simple and sensitive spectrophotometric determination of Ziram, zineb and ferbam in commercial samples and foodstuffs using phenylfluorone.
Journal of environmental monitoring : JEM, 2000Co-Authors: Ashok Kumar Malik, Jyoti Kapoor, A. L. J. RaoAbstract:A procedure has been developed for the determination of Ziram, zineb and ferbam dithiocarbamate pesticides by converting Ziram and zineb into a zinc-phenylfluorone complex and ferbam into an iron phenylfluorone complex, which are then dissolved in water in the presence of cetylpyridinium bromide and pyridine as a surfactant. The method is sensitive, highly selective and can be used for the determination of Ziram, zineb and ferbam in commercial samples and in foodstuffs.
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SPECTROPHOTOMETRIC DETERMINATION OF Ziram AND ZINEB USING 4-(2-PYRIDYLAZO)RESORCINOL
Pesticide Science, 1994Co-Authors: Jyoti Kapoor, A. L. J. RaoAbstract:A spectrophotometric method has been developed for the determination of Ziram (zinc bis(dimethyldithiocarbamate)) and zineb (zinc ethylenebisdithiocarbarnate) by converting these into a zinc-4-(2-pyridylazo)resorcinol complex, which is dissolved in acetone and water and the absorbance measured at 490 nm against a reagent blank. Beer's law is obeyed over the concentration ranges 0.025-1.25 and 0.025-1.5 mg litre−1 for Ziram and zineb respectively in the final solution. The method is sensitive and can be used for the direct determination of Ziram and zineb in commercial formulations, grains (rice and wheat) and synthetic mixtures (in the presence of various other dithiocarbamates). The limit of determination of Ziram and zineb from foodstuffs in 0.2 mg kg −1.
Ashok Kumar Malik - One of the best experts on this subject based on the ideXlab platform.
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spectrophotometric determination of Ziram and zineb in commercial samples and food stuffs using par naphthalene as column adsorbate for preconcentration
2012Co-Authors: Shivender Singh Saini, Vanita Sharma, Ashok Kumar MalikAbstract:A procedure has been developed for the determination of zinc(II) bis (dimethyldithiocarbamate) (Ziram) and zinc(II) ethylenebisdithiocarbamate (Zineb) after preconcentration on a column using naphthalene-PAR as adsorbent. Ziram and Zineb are quantitatively retained on the column in the pH range 9.0- 12.5 and at a flow rate of 1-2 ml/min. The solid mass consisting of the Zn-PAR complex along with naphthalene is dissolved from the column with 5 ml of dimethylformamide (DMF). The absorbance is measured at 490 nm with a spectrophotometer against the reagent blank. Beer's law is obeyed over the concentration range 0.1- 15 µg of Ziram and 0.1 -13 µg of zineb in 5 ml of the final DMF solution. Ten replicate determinations on a sample solution containing 40 µg of Ziram and 36 µg of zineb gave a mean absorbance of 0.30 with a relative standard deviation 0.98%. The interference of various ions has been studied and the method has been employed to the determination of Ziram and zineb in commercial samples and in various foodstuffs.
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Development of new adsorbent chitin for column preconcentration and spectrophotometric trace determination of Ziram and Zineb in synthetic, commercial samples and food-stuffs.
Talanta, 2005Co-Authors: S. K. Mehta, Ashok Kumar Malik, Baldev Singh, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (Ziram) or zinc(II) ethylenebisdithiocarbamate (Zineb) present in a large volume of aqueous solution after preconcentration on a column using chitin-1-(2'pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram/Zineb are quantitatively retained on the column as Zn-PAN complex in the pH range 9.0-11.0 and at a flow rate of 1-8 ml/min. Complex adsorbed on chitin was eluted from the column with dimethylformamide (DMF) and absorbance of the eluate was measured at 550 nm against a reagent blank. Beer's law is obeyed over the concentration range 5.3-55.8 microg of Ziram and 6.8-49.0 microg of Zineb in 25 ml of the final DMF solution. Ten replicate determinations on a sample solution containing 45.22 microg of Ziram or 40.86 microg of Zineb gave a mean absorbance of 0.30 with a relative standard deviation 1.6 and 1.8%, respectively. The interference of various ions has been studied. Many alkali metals and metal salts do not interfere. The method has been employed to the determination of Ziram and Zineb in commercial samples and in various foodstuffs and the results were compared with the earlier reported methods.
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column preconcentration and spectrophotometric trace determination of Ziram and zineb using chitin as an adsorbate
Indian journal of chemistry. Sect. A: Inorganic physical theoretical & analytical, 2005Co-Authors: S. K. Mehta, Ashok Kumar Malik, Baldev Singh, U. Gupta, Lj A RaoAbstract:A new method has been developed for the determination of Ziram and Zineb present in a large volume of aqueous solution by complex formation on a natural polymer chitin loaded with 4-(2'-pyridylazo)resorcinol. Ziram/Zineb is quantitatively retained on the column and exchange the zinc present in them with PAR in the pH range 9.0-12.5 at a flow rate of 1-10 ml/min. The complex adsorbed on chitin is eluted from the column with 10 ml of dimethylformamide and absorbance of the eluate measured at 490 nm against a reagent blank. The molar absorptivity is found to be 9.2 x 10 4 1 mol - 1 cm - 1 . Sandell's sensitivity for Ziram and Zineb is found to be 0.0033 and 0.0029 μg cm 2 , respectively. The method can be applied for the determination of Ziram and Zineb in synthetic, commercial samples and in various foodstuffs.
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Fourth derivative spectrophotometric determination of fungicide Ziram (zinc(II) dimethyldithiocarbamate) in commercial samples and wheat grains
International Journal of Environmental Analytical Chemistry, 2004Co-Authors: Vaneet K Sharma, Ashok Kumar Malik, Jatinder Singh Aulakh, Sonam Bansal, Rakesh Kumar MahajanAbstract:A procedure has been developed for the direct fourth derivative spectrophotometric determination of zinc(II) dimethyldithiocarbamate by converting it into its copper(II) dimethyldithiocarbamate complex, which is then dissolved in Triton X-100. Beer's law is obeyed over the concentration range 0.5–30 µg/mL in the final solution. Various parameters such as the effect of pH and the interference of a number of ions on the determination of Ziram have been studied in detail. The method is sensitive and can be used for the determination of Ziram in commercial samples like Zirax and Ziron containing Ziram and from wheat grains.
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column preconcentration and spectrophotometric determination of Ziram and zineb in commercial samples and foodstuffs using 1 2 pyridylazo 2 naphthol pan naphthalene as adsorbate
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Ashok Kumar Malik, Vanita Sharma, Vaneet K Sharma, A. L. J. RaoAbstract:A procedure has been developed for the determination of zinc(II) bis(dimethyldithiocarbamate) (Ziram) and zinc(II) ethylenebis(dithiocarbamate) (zineb) after preconcentration on a column using naphthalene-(1,2‘-pyridylazo)-2-naphthol (PAN) as adsorbent. Ziram and zineb are quantitatively retained on the column in the pH range of 9.0−12.5 and at a flow rate of 1−2 mL/min. The solid mass consisting of the Zn−PAN complex along with naphthalene is dissolved from the column with 5 mL of dimethylformamide (DMF). Absorbance of the complex was measured at 550 nm; Beer's law is obeyed over the concentration ranges of 2.0−22.0 μg of Ziram and 5.0−19.8 μg of zineb in 10 mL of the final DMF solution. Ten replicate determinations on a sample solution containing 20 μg of Ziram and 18 μg of zineb gave a mean absorbance of 0.33 with relative standard deviations of 0.80 and 0.70%, respectively. The interference of various ions has been studied. The method has been employed for the determination of Ziram and zineb in comme...
Benavent Oltra, Sara Ester - One of the best experts on this subject based on the ideXlab platform.
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Desarrollo de un nuevo procedimiento para la determinación del plaguicida Ziram mediante la utilización de técnicas de análisis en flujo continuo y detección quimioluminiscente
'Universitat Politecnica de Valencia', 2014Co-Authors: Benavent Oltra, Sara EsterAbstract:Los objetivos que se pretenden son: Desarrollar un método analítico para la determinación del plaguicida Ziram. Evaluar las características analíticas del método desarrollado. Aplicar dicho método a la determinación del plaguicida en muestras reales.Benavent Oltra, SE. (2007). Desarrollo de un nuevo procedimiento para la determinación del plaguicida Ziram mediante la utilización de técnicas de análisis en flujo continuo y detección quimioluminiscente. http://hdl.handle.net/10251/38092Archivo delegad
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Desarrollo de un nuevo procedimiento para la determinación del plaguicida Ziram mediante la utilización de técnicas de análisis en flujo continuo y detección quimioluminiscente
'Universitat Politecnica de Valencia', 2014Co-Authors: Benavent Oltra, Sara EsterAbstract:Los objetivos que se pretenden son: Desarrollar un método analítico para la determinación del plaguicida Ziram. Evaluar las características analíticas del método desarrollado. Aplicar dicho método a la determinación del plaguicida en muestras reales.Benavent Oltra, SE. (2007). Desarrollo de un nuevo procedimiento para la determinación del plaguicida Ziram mediante la utilización de técnicas de análisis en flujo continuo y detección quimioluminiscente. Universitat Politècnica de València. http://hdl.handle.net/10251/38092Archivo delegad
