The Experts below are selected from a list of 984 Experts worldwide ranked by ideXlab platform
Aruna Bhatia - One of the best experts on this subject based on the ideXlab platform.
-
Antiplasmodial effects of the aqueous ethanolic seed extract of Ziziphus mauritiana against Plasmodium berghei in Swiss albino mice
Bharati Biomedical and Advance Research Centre, 2014Co-Authors: Tulika Mishra, Aruna BhatiaAbstract:Ziziphus mauritiana is a fruit tree used traditionally since long back for wound healing, immunepotentiator, asthma, sedative, stomachic, styptic, as tonic etc. The present study determines the antiplasmodial effect of aqueous ethanolic seed extract against Chloroquine sensitive Plasmodium berghei berghei nk65 infection in Swiss albino mice. Based upon the acute toxicity data three different doses (100, 200, 400 mg/kg body weight) of the plant extract was chosen to study the blood schizonticidal activity in early infection and in established infection and was compared with chloroquine. The Prophylactic activity was also assessed and compared with pyrimethamine. No mortality was observed in acute toxicity study however, above the dose of 1000 mg/kg animals showed the lethargic behaviour. In early infection, and in established infection the doses (100-400 mg/kg b.wt) was found to cause significant (P
-
anticancer potential of aqueous ethanol seed extract of Ziziphus mauritiana against cancer cell lines and ehrlich ascites carcinoma
Evidence-based Complementary and Alternative Medicine, 2011Co-Authors: Tulika Mishra, Madhu Khullar, Aruna BhatiaAbstract:Ziziphus mauritiana (Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 g/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.
-
hypoglycemic activity of Ziziphus mauritiana aqueous ethanol seed extract in alloxan induced diabetic mice
Pharmaceutical Biology, 2010Co-Authors: Aruna Bhatia, Tulika MishraAbstract:Ziziphus mauritiana Lamk. (Rhamnaceae) is a fruit tree that has been used as folkloric medicine for many ailments and diseases. In the present study, the hypoglycemic effect of seed extract of Ziziphus mauritiana in alloxan-induced diabetic mice was assessed. Seed extract was administered orally at doses of 100, 400, and 800 mg/kg body weight (bw) and also in combination with glyburide (800 mg/kg seed extract and 10 mg/kg glyburide) to different groups of mice (normal and alloxan-treated diabetic mice). Their blood glucose level (in acute and sub-acute study), body weight, and mortality rate were monitored. Oral glucose tolerance test (OGTT) was also performed. Oral administration of extract alone or in combination with glyburide reduced the blood glucose level in all the diabetic mice after acute and sub-acute (28 days) administration. Administration of the extract reduced the weight loss and mortality rate during the sub-acute study. The results of blood glucose level, loss in body weight, and mortality...
-
augmentation of expression of immunocytes functions by seed extract of Ziziphus mauritiana lamk
Journal of Ethnopharmacology, 2010Co-Authors: Tulika Mishra, Aruna BhatiaAbstract:Abstract Aim of the study The present study was carried out to evaluate the immunomodulatory potential of Ziziphus mauritiana (Lamk.) seed extract to ascertain the folkloric claim as immunomodulator. Materials and methods The aqueous-ethanolic seed extract (100–400 mg kg−1) of Z. mauritiana was investigated for immunomodulatory potential in mice. The extract was standardized with HPLC using betulinic acid as a marker. Functions of various immunocytes in the form of humoral (development of anti-SRBC (sheep red blood cells) antibody titers) and cell-mediated immune response (delayed type hypersensitivity, nitroblue tetrazolium reduction, inducible nitric oxide synthase activity and bactericidal activity) was studied in SRBC immunized mice. The cytokine, IFN-γ (interferon-gamma) and IL-4 (interleukin-4) secretion was also measured quantitatively by ELISA as the expression of functions of Th-1 and Th-2 respectively. Levamisole (2.5 mg kg−1) was used as standard drug. Results The seed extract demonstrated significant (P Conclusion The immunostimulatory potential of this seed extract is likely to be mediated through its effect on macrophage function and Th-1 mediated immunity confirming the folkloric use of this plant.
Tulika Mishra - One of the best experts on this subject based on the ideXlab platform.
-
Antiplasmodial effects of the aqueous ethanolic seed extract of Ziziphus mauritiana against Plasmodium berghei in Swiss albino mice
Bharati Biomedical and Advance Research Centre, 2014Co-Authors: Tulika Mishra, Aruna BhatiaAbstract:Ziziphus mauritiana is a fruit tree used traditionally since long back for wound healing, immunepotentiator, asthma, sedative, stomachic, styptic, as tonic etc. The present study determines the antiplasmodial effect of aqueous ethanolic seed extract against Chloroquine sensitive Plasmodium berghei berghei nk65 infection in Swiss albino mice. Based upon the acute toxicity data three different doses (100, 200, 400 mg/kg body weight) of the plant extract was chosen to study the blood schizonticidal activity in early infection and in established infection and was compared with chloroquine. The Prophylactic activity was also assessed and compared with pyrimethamine. No mortality was observed in acute toxicity study however, above the dose of 1000 mg/kg animals showed the lethargic behaviour. In early infection, and in established infection the doses (100-400 mg/kg b.wt) was found to cause significant (P
-
anticancer potential of aqueous ethanol seed extract of Ziziphus mauritiana against cancer cell lines and ehrlich ascites carcinoma
Evidence-based Complementary and Alternative Medicine, 2011Co-Authors: Tulika Mishra, Madhu Khullar, Aruna BhatiaAbstract:Ziziphus mauritiana (Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 g/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.
-
hypoglycemic activity of Ziziphus mauritiana aqueous ethanol seed extract in alloxan induced diabetic mice
Pharmaceutical Biology, 2010Co-Authors: Aruna Bhatia, Tulika MishraAbstract:Ziziphus mauritiana Lamk. (Rhamnaceae) is a fruit tree that has been used as folkloric medicine for many ailments and diseases. In the present study, the hypoglycemic effect of seed extract of Ziziphus mauritiana in alloxan-induced diabetic mice was assessed. Seed extract was administered orally at doses of 100, 400, and 800 mg/kg body weight (bw) and also in combination with glyburide (800 mg/kg seed extract and 10 mg/kg glyburide) to different groups of mice (normal and alloxan-treated diabetic mice). Their blood glucose level (in acute and sub-acute study), body weight, and mortality rate were monitored. Oral glucose tolerance test (OGTT) was also performed. Oral administration of extract alone or in combination with glyburide reduced the blood glucose level in all the diabetic mice after acute and sub-acute (28 days) administration. Administration of the extract reduced the weight loss and mortality rate during the sub-acute study. The results of blood glucose level, loss in body weight, and mortality...
-
augmentation of expression of immunocytes functions by seed extract of Ziziphus mauritiana lamk
Journal of Ethnopharmacology, 2010Co-Authors: Tulika Mishra, Aruna BhatiaAbstract:Abstract Aim of the study The present study was carried out to evaluate the immunomodulatory potential of Ziziphus mauritiana (Lamk.) seed extract to ascertain the folkloric claim as immunomodulator. Materials and methods The aqueous-ethanolic seed extract (100–400 mg kg−1) of Z. mauritiana was investigated for immunomodulatory potential in mice. The extract was standardized with HPLC using betulinic acid as a marker. Functions of various immunocytes in the form of humoral (development of anti-SRBC (sheep red blood cells) antibody titers) and cell-mediated immune response (delayed type hypersensitivity, nitroblue tetrazolium reduction, inducible nitric oxide synthase activity and bactericidal activity) was studied in SRBC immunized mice. The cytokine, IFN-γ (interferon-gamma) and IL-4 (interleukin-4) secretion was also measured quantitatively by ELISA as the expression of functions of Th-1 and Th-2 respectively. Levamisole (2.5 mg kg−1) was used as standard drug. Results The seed extract demonstrated significant (P Conclusion The immunostimulatory potential of this seed extract is likely to be mediated through its effect on macrophage function and Th-1 mediated immunity confirming the folkloric use of this plant.
Alhasan M Wudil - One of the best experts on this subject based on the ideXlab platform.
-
investigation of cytotoxicity potential of different extracts of Ziziphus mauritiana lam leaf allium cepa model
Toxicology reports, 2020Co-Authors: Tajudeen A Owolarafe, K Salawu, Godwin O Ihegboro, Chimaobi J Ononamadu, A J Alhassan, Alhasan M WudilAbstract:Previous studies showed that Ziziphus mauritiana is ethnomedicinally beneficial against various diseases, however the cytotoxic and genotoxic effects of this plant have not been well investigated. Therefore this study was undertaken to investigate cytotoxic and genotoxic effects of four different solvents extracts of Ziziphus mauritiana (Lam) leaf at different concentrations (20, 40, 60, 80, and 100 mg/L) using Allium cepa model. The cytotoxicity and genotoxicity parameters evaluated were mitotic index, root tip growth length and chromosomal aberration respectively. The result revealed a decrease in mitotic index percentage (%MI) and a dose dependent decrease in root tip length with increase in extracts concentration for all the extracts studied - with the ethanol extract showing the most significant effect in mitotic index. Furthermore, the effective concentrations (EC50) obtained were 81.30, 52.01, 90.68 and 112.30 mg/l for aqueous, ethanol, ethyl acetate and hexane extract respectively. Finally chromosomal aberrations such as vagrant chromosome, c-mitosis, bridged anaphase, sticky telophase were also observed in all four extracts and the percentage chromosomal aberration were observed to decrease with increased concentrations of extracts. Therefore based on the result obtained in this study it may be concluded that the plant (Ziziphus mauritiana (Lam)) extracts are cytotoxic and genotoxic in nature and the observed decrease in percentage chromosomal aberration may be as a result of antimutagenic bioactive principles present in the plant extracts. Hence care must be taken in its consumption and use in folk medicine.
D Dahiru - One of the best experts on this subject based on the ideXlab platform.
-
Effect of Aqueous Extract of Ziziphus mauritiana Leaf on Cholesterol and Triglyceride Levels in Serum and Liver of Rats Administered Alcohol
2015Co-Authors: D Dahiru, O ObidoaAbstract:Abstract: Effects of aqueous extract of Ziziphus mauritiana (Zm) leaf on serum and liver cholesterol and triacylglycerol were studied in chronic alcohol administered rats. Pretreatment co-administration and post treatment protocols were employed. Rats fed alcohol only for 6 weeks had significantly (p<0.05) elevated levels of both serum and liver cholesterol and triglyceride. Pretreatment of rats with 400 mg/kg bw of aqueous extract of Zm leaf prior to alcohol administration significantly (p<0.05) inhibited both levels of serum and liver cholesterol and triglyceride. Co-administration of alcohol with 400 mg/kg bw aqueous extract of Zm leaf significantly (p<0.05) inhibited serum triglyceride level only. Post treatment of alcohol fed rats with 400 mg/kg aqueous extract of Zm for 2 weeks significantly (p<0.05) reduced the levels of both serum and liver cholesterol and triglyceride. Aqueous extract of Zm leaf can be used for the prevention and treatment of fatty liver, atherosclerosis and other diseases associated with high levels of cholesterol and triglyceride. Pretreatment was found to confer more protection than co-treatment, hence pretreatment should be preferred. Key words: Ziziphus mauritiana, alcohol, cholesterol, triglycerid
-
Ziziphus mauritiana fruit extract inhibits carbon tetrachloride induced hepatotoxicity in male rats
Pakistan Journal of Nutrition, 2010Co-Authors: D Dahiru, D N Mamman, H Y WakawaAbstract:The aqueous extract of Ziziphus mauritiana fruit (Zm) was evaluated for its protective activity against CCl4-induced liver damage. 250, 500 mg/kg bw of Zm fruit extract or 100 mg/kg silymarin (standard) were administered to different groups of rats prior to CCl4 administration. Both 250 and 500 mg/kg bw of Zm fruit extract significantly (p<0.05) reduced (dose dependently) the levels of enzymes and non-enzymes markers of tissue damage when compared to rats given CCl4 only. These findings were supported by liver histology and suggest that Zm fruit possessed reach hepatoprotective principles that inhibited the toxicity of CCl4 against the liver.
-
evaluation of the antioxidant effects of Ziziphus mauritiana lam leaf extracts against chronic ethanol induced hepatotoxicity in rat liver
African Journal of Traditional Complementary and Alternative Medicines, 2008Co-Authors: D Dahiru, O ObidoaAbstract:Chronic alcohol ingestion is known to increase the generation of reactive oxygen species (ROS), thereby leading to liver damage. Antioxidant enzymes act individually or in combination to reduce or counter the effect of these ROS. Chronic administration of alcohol at (40% v/v, 1ml/100g), for 6 weeks showed a significant (p<0.05) elevated levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TB). There was also a significant (p<0.05) decreased levels of catalase, glutathione peroxidase, glutathione reductase and superoxide dismutase compared to control rats. Pre-treatment of rats with 200, 400 mg/kg body weight of aqueous leaf extract of Ziziphus mauritiana or 100 mg/kg silymarin resulted in a significant (p<0.05) decreased levels of ALT, AST, ALP, and TB with levels of catalase, glutathione peroxidase, glutathione reductase and superoxide dismutase showing a significant (p<0.05) increase compared to group administered alcohol only. Histopathology of rat liver administered with alcohol only resulted in severe necrosis, mononuclear cell aggregation and fatty degeneration in the central and mid zonal areas which was a characteristic of a damaged liver. Pre-treatment with the aqueous extract of Ziziphus mauritiana or silymarin reduced the morphological changes that are associated with chronic alcohol administration. The presence of tannins, saponins and phenolic compounds observed in the plant extract could be responsible for the observed effects of decreasing the levels of injured tissue marker and lipid peroxidation.
-
pretreatment of albino rats with aqueous leaf extract of Ziziphus mauritiana protects against alcohol induced liver damage
Tropical Journal of Pharmaceutical Research, 2007Co-Authors: D Dahiru, O ObidoaAbstract:Purpose: The effect of the aqueous extract of Ziziphus mauritiana leaf on hepatic lipid peroxidation, reduced glutathione and total antioxidant status was studied in chronic alcohol-induced liver damage. Method: Alcohol-induced liver toxicity was created by oral administration of 40% alcohol solution (v/v, 1ml/100g) to rats for 6 weeks. Other groups of rats were pretreated with 200 and 400 mg/kg bw aqueous extracts of Ziziphus mauritiana leaf or 100 mg/kg bw silymarin (reference drug) 30 min prior to alcohol ingestion. Body weight of rats was monitored weekly. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin and liver total antioxidant status, reduced glutathione and lipid peroxidation were evaluated. Result: Rats that received alcohol only showed significantly (p<0.05) elevated levels of ALT, AST, bilirubin, and hepatic lipid peroxidation while reduced glutathione, total antioxidant status and body weight significantly (p<0.05) decreased compared to control rats. Pretreatment of rats with aqueous extract of Ziziphus mauritiana 30 min prior to alcohol administration resulted in significant (p<0.05) depression of ALT, AST, bilirubin and lipid peroxidation levels compared to the group exposed to alcohol only. Administration of Ziziphus mauritiana extract prior to alcohol ingestion significantly (p<0.05) resulted in increased levels of reduced glutathione and total antioxidant status compared to the group that received alcohol only. Conclusion: The results of this study indicate that the aqueous extract of Ziziphus mauritiana leaf may prevent chronic alcohol-induced liver injury by enhancing the levels of total antioxidant status and inhibiting hepatic lipid peroxidation.
-
antidiarrhoeal activity of Ziziphus mauritiana root extract in rodents
African Journal of Biotechnology, 2006Co-Authors: D Dahiru, J M Sini, L JohnafricaAbstract:Preliminary phytochemical screening of the root extract of Ziziphus mauritiana revealed the presence of alkaloids, flavonoids, glycosides, saponins and volatile oil. Intraperitoneal LD50 of the extract was found to be 447.21 ± 20 mg/kg (bw) in mice. The Antidiarrhoeal effect of the methanolic extract as evaluated exhibited a concentration dependent inhibition of the spontaneous pendular movement of the isolated rabbit jejunum and inhibited acetylcholine induced contraction of rat ileum. A dose dependent decrease of gastrointestinal transit was observed with extracts (25 and 50 mg/kg) which also protected mice against castor oil induced dirrhoea and castor oil induced fluid accumulation, respectively. The presence of some of the phytochemicals in the root extract may be responsible for the observed effects, and also the basis for its use in traditional medicine as antidiarrhoeal drug
Muhammad S Sule - One of the best experts on this subject based on the ideXlab platform.
-
EVALUATION OF HYPOGLYCAEMIC, HYPOLIPIDAEMIC AND NON TOXIC EFFECT OF HYDRO-METHANOLIC EXTRACTS OF Ziziphus mauritiana, Ziziphus SPINA CHRISTI FRUIT AND GLIBENCLAMIDE ON ALLOXAN INDUCED DIABETIC RATS
Journal of Drug Delivery and Therapeutics, 2018Co-Authors: Salisu Abubakar, Sufyan A Umar, Idoko Alexander, Nura Abubakar, Mansurah A. Abdulazeez, Muhammad S SuleAbstract:The use of plant products in the management of diabetes has gained ground in pharmacotherapy. It becomes imperative to evaluate the antidiabetic effects of fruit extracts of Ziziphus mauritiana (HMZM), Ziziphus spina christi (HMZS) and Glibenclamide on blood glucose, total protein, albumin and lipid profile in alloxan induced diabetic rats. 68 albino rats weighing 70 - 130g were used in this study. 26 rats were used for acute toxicities of Ziziphus mauritiana and Ziziphus spina christi. 42 rats of 7 groups of 6 rats each were used to test antidiabetic effects of Ziziphus mauritiana and Ziziphus spina christi plant extracts. Group 1 served as the negative control, groups 2-7 were intraperitoneally administered 360mg/kg of alloxan in normal saline respectively. Group 2 served as positive control, groups 3 and 4; 5 and 6 were respectively administered daily dose of 200 and 400mg/kg of HMZM and HMZS, group 7 was administered 0.21mg/kg of glibenclamide. Results showed Ziziphus mauritiana and Ziziphus spina christi non toxic at dose of 5000mg/kg. 48 hours after alloxan administration, blood glucose levels were found to be significantly higher (P 0.05) serum level of total protein and albumin compared to group 2. The results obtained showed a significantly lower (P < 0.05) serum level of glucose. The effects of HMZMand HMZS fruit extracts on blood glucose, total protein, albumin and lipid profile was dose dependent. Conclusively, this study has demonstrated the antidiabetic effects of HMZM and HMZS with HMZS having a more pronounced effect on Blood glucose and lipid profile. Keywords: Antidiabetic, Blood glucose, lipid profile, Glibenclamide, Ziziphus mauritiana, Ziziphus spina Christi.
