The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform
Vikas V. Vaidya - One of the best experts on this subject based on the ideXlab platform.
-
Asimple, selective validatedmethod for simultaneous estimation of Zuclomifene and enclomifene in human plasma by liquid chromatography - tandemmass spectrometry
Analytical chemistry an Indian journal, 2011Co-Authors: Arvind G. Jangid, Rajesh H. Tale, Vikas V. VaidyaAbstract:Asimple and selectivemethod for estimation of Zuclomifene and enclomifene in human plasma was developed and validated using glipizide as internal standard. The analytes were chromatographed onACQUITY UPLC® BEH C18 1.7µ (2.1 mm x 100 mm) reverse phase column under isocratic conditions using 5mMAmmoniumAcetate inwater (pH4.00 ± 0.05): methanol (38:62 v/ v) as the mobile phase and detected by tandem mass spectrometry in positive ion mode. The ion transition recorded in multiple reaction monitoring mode were m/z 406.2i 100.0 for Zuclomifene and enclomifene and m/z 446.1i 321.0 for internal standard. Simple solid phase extraction method used for extraction of analytes and internal standard from plasma. Linearity in plasma was observed over the concentration range 0.102  15.225 ng/mL for Zuclomifene and 0.149  22.275 ng/mL for enclomifene. The method was validated for sensitivity, matrix effect, accuracy and precision, linearity, recovery and stability studies. The lower limit of quantification of Zuclomifene and enclomifene was 0.102 ng/mL and 0.149 ng/mLrespectively. The mean recovery for Zuclomifene and enclomifene was 88.2 % and 85.7 % respectively. The coefficient of variation of the assaywas less than 11.7%and 12.3 %and accuracyof 91.3%to 105.7%and 91.2%to 106.6%for Zuclomifene and enclomifene respectively. The validated method can be applied to pharmacokinetic study of clomifene.
Arvind G. Jangid - One of the best experts on this subject based on the ideXlab platform.
-
Asimple, selective validatedmethod for simultaneous estimation of Zuclomifene and enclomifene in human plasma by liquid chromatography - tandemmass spectrometry
Analytical chemistry an Indian journal, 2011Co-Authors: Arvind G. Jangid, Rajesh H. Tale, Vikas V. VaidyaAbstract:Asimple and selectivemethod for estimation of Zuclomifene and enclomifene in human plasma was developed and validated using glipizide as internal standard. The analytes were chromatographed onACQUITY UPLC® BEH C18 1.7µ (2.1 mm x 100 mm) reverse phase column under isocratic conditions using 5mMAmmoniumAcetate inwater (pH4.00 ± 0.05): methanol (38:62 v/ v) as the mobile phase and detected by tandem mass spectrometry in positive ion mode. The ion transition recorded in multiple reaction monitoring mode were m/z 406.2i 100.0 for Zuclomifene and enclomifene and m/z 446.1i 321.0 for internal standard. Simple solid phase extraction method used for extraction of analytes and internal standard from plasma. Linearity in plasma was observed over the concentration range 0.102  15.225 ng/mL for Zuclomifene and 0.149  22.275 ng/mL for enclomifene. The method was validated for sensitivity, matrix effect, accuracy and precision, linearity, recovery and stability studies. The lower limit of quantification of Zuclomifene and enclomifene was 0.102 ng/mL and 0.149 ng/mLrespectively. The mean recovery for Zuclomifene and enclomifene was 88.2 % and 85.7 % respectively. The coefficient of variation of the assaywas less than 11.7%and 12.3 %and accuracyof 91.3%to 105.7%and 91.2%to 106.6%for Zuclomifene and enclomifene respectively. The validated method can be applied to pharmacokinetic study of clomifene.
Rajesh H. Tale - One of the best experts on this subject based on the ideXlab platform.
-
Asimple, selective validatedmethod for simultaneous estimation of Zuclomifene and enclomifene in human plasma by liquid chromatography - tandemmass spectrometry
Analytical chemistry an Indian journal, 2011Co-Authors: Arvind G. Jangid, Rajesh H. Tale, Vikas V. VaidyaAbstract:Asimple and selectivemethod for estimation of Zuclomifene and enclomifene in human plasma was developed and validated using glipizide as internal standard. The analytes were chromatographed onACQUITY UPLC® BEH C18 1.7µ (2.1 mm x 100 mm) reverse phase column under isocratic conditions using 5mMAmmoniumAcetate inwater (pH4.00 ± 0.05): methanol (38:62 v/ v) as the mobile phase and detected by tandem mass spectrometry in positive ion mode. The ion transition recorded in multiple reaction monitoring mode were m/z 406.2i 100.0 for Zuclomifene and enclomifene and m/z 446.1i 321.0 for internal standard. Simple solid phase extraction method used for extraction of analytes and internal standard from plasma. Linearity in plasma was observed over the concentration range 0.102  15.225 ng/mL for Zuclomifene and 0.149  22.275 ng/mL for enclomifene. The method was validated for sensitivity, matrix effect, accuracy and precision, linearity, recovery and stability studies. The lower limit of quantification of Zuclomifene and enclomifene was 0.102 ng/mL and 0.149 ng/mLrespectively. The mean recovery for Zuclomifene and enclomifene was 88.2 % and 85.7 % respectively. The coefficient of variation of the assaywas less than 11.7%and 12.3 %and accuracyof 91.3%to 105.7%and 91.2%to 106.6%for Zuclomifene and enclomifene respectively. The validated method can be applied to pharmacokinetic study of clomifene.
