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Gato, Worlanyo E. - One of the best experts on this subject based on the ideXlab platform.

  • Investigating the Toxic Effects of 2-Aminoanthracene Ingestion in Pregnant Sprague Dawley Dams
    'Informa UK Limited', 2018
    Co-Authors: Wilson, Hannah K., Yau Wilson, Howerth, Elizabeth W., Buckeridge, Steven A., Gato, Worlanyo E.
    Abstract:

    Polycyclic aromatic hydrocarbons (PAHs) refer to organic compounds that are byproducts of incomplete combustion of fossil fuels and wood. One specific polycyclic aromatic hydrocarbon, 2-Aminoanthracene (2AA), is a member of a broader group of compounds known as anthracenes, which have been classified by the United States Agency for Toxic Substances and Disease Registry (ASTDR) as one of a group of PAHs of top concern based on their greater potential risk for exposure and greater harmful effects to humans, compared to other PAHs. Previous research has shown that 2AA affects genes involved in carbohydrate and lipid metabolism, inflammatory stress responses, and immune system responses, among other processes. The objective of the present study was to examine the toxicity of dietary ingestion of 2AA from gestation through the postnatal period. Pregnant dams (Day 1) were purchased from Taconic Hudson, NY, and assigned into dose regimens of 0 mg/kg- (control-C), 50 mg/kg- (low dose-LD) and 100 mg/kg-diet (high dose-HD) 2AA. Dams were fed 2AA contaminated diet during the period of gestation and postpartum. Insulin and H&E immunohistochemical staining were undertaken and indicated no significant changes between control and treated groups. However, percent pancreatic islets (islets within the pancreas) were larger in the exposed groups. The value was 1.5% in the control dams compared to 3.2% and 4.3% low dose and high dose groups respectively. Serum concentrations of albumin and lactate dehydrogenase (LDH) were increased in the exposed groups, with the HD group experiencing the greater increase. Analyses of Fabp4, Mgmt , Fas, Nhej1, Aldh1a1 and Ncam1 were conducted via real-time quantitative polymerase chain reaction (RT-pPCR), using β-Actin as the control gene. There was an up-regulation of the Mgmt and Nhej1 gene transcripts in the exposed groups, with the extent of upregulation being highest in the HD group. Taken together, a link between environmental exposure to 2AA and pancreatic effects appears to exist

  • Examining the Hepatic Effects of In Utero Exposure to 2-Amionanthracene
    'Society of Toxicology India', 2017
    Co-Authors: Barnett, Grant W., Yau Wilson, Rasdall, Jessica A., Bartel, Leah K., Gato, Worlanyo E.
    Abstract:

    The extreme prevalence of diabetes in the United States has not always existed; the number of cases has dramatically risen in recent history. Previous research has indicated 2-Aminoanthracene (2AA) causing alteration of gene expression and systemic injury in adult rats. This project determines the effects of 2AA on the in utero pups after being ingested by the dams. The goal was to identify any systemic injuries or effects on the in utero pups after ingestion of 2AA by their dams. This information is useful to determine the possible effects of pregnant humans ingesting 2AA, which is a compound that could easily be ingested in large quantities because of its prevalence in products and prepared food. 2AA could have potential effects on the pregnant mother and her unborn child. 2AA may cause alteration in gene expression or systemic injury that could last into adulthood and encourage or even cause insulin resistance or Type-II diabetes (T2D). Weight gain differed between the test groups and the control group. The low dose of 2AA promoted weight gain and the high dose inhibited. No significant difference in CD68+ (cluster differentiation 68) cell count between the treated and untreated groups occurred. Alteration in gene expression because of 2AA exposure was found. mRNA quantification revealed that adenosine monophosphate-activated protein kinase (AMPK), fatty acid synthase(FASN) and peroxisome proliferatoractivated receptor alpha (PPARA) were not expressed, while cluster of differentiation 36 (CD36), hepatocyte nuclear factor 4 (HNF4), phosphoenolpyruvate carboxykinase 1(PCK1) and suppressor of cytokine signaling 1(SOCS1) were upregulated. Findings from this study suggest intrauterine exposure to polycyclic aromatic hydrocarbons (PAHs), particular 2-Aminoanthracene, may lead to susceptibility to T2D

  • Assessment of the Link Between In Utero Exposure to 2-Aminoanthracene (2AA) and Type-1 Diabetes (T1D)
    'Springer Science and Business Media LLC', 2017
    Co-Authors: Mays, Christopher A., Yau Wilson, Hunter, Daniel A., Gato, Worlanyo E.
    Abstract:

    Background: A recent diabetes report revealed an increased incidence in diabetes including type 1-diabetes (T1D). The increase in the numbers of T1D incidences are thought to be related to environmental reasons such as the exposure to environmental chemicals including arylamine 2-Aminoanthracene (2AA). T1D is an autoimmune disease of the pancreatic islet in which insulin-producing beta cells are destroyed by auto-reactive T-cells and monocytic cells. Methods: The purpose of this study is to examine the extent to which 2AA exposure contributes to T1D. Three groups of pregnant Sprague Dawley dams ingested various concentrations of dietary 2AA from gestation through the postnatal period. A select number of cytokines and adipokines previously noted to play a significant role in inflammatory response were analyzed in the pancreas of the pups for alteration. The anatomy of the pancreas was also evaluated to determine any histological changes. Results: Results showed over-expression of pro-inflammatory protein IL-6. Up-regulation of humoral genes IL-7 and IL-21 were also noted. Pathologic characterization showed no significant changes. Moreover, serum total protein was significantly reduced in exposed groups. Elevated serum glucose concentration seems to correspond to slightly lower insulin levels in serum. Cumulative neonatal weight gain analysis showed no major alterations between the control and gestationally-exposed rats. Conclusion: It appears that systemic effects of 2AA ingestion were mild in the neonates. Further assessments of pups who lived longer than two weeks could be a useful way to measure the progression and possibly further support our hypothesis that 2AA can lead to systemic effects that are indicative of inducing T1D

  • Original Article. Inflammatory effect of 2-Aminoanthracene (2AA) on adipose tissue gene expression in pregnant Sprague Dawley rats
    Sciendo, 2016
    Co-Authors: Whitby, Shamaya L., Yau Wilson, Howerth, Elizabeth W., Hunter, Daniel A., Gato, Worlanyo E.
    Abstract:

    Adipocyte dysfunction may be a critical link between obesity and insulin resistance as a result of abnormal fat storage and mobilization. Adipocytes uniquely secrete adipokines and cytokines, such as leptin and TNFα, wich promote insulin sensitivity. Previously we reported insulin-signaling related altered gene expression in animals exposed to 2-Aminoanthracene (2AA). 2AA is an aminosubstituted polycyclic aromatic hydrocarbon used in manufacturing dyes, chemicals, inks, resins, and polyurethanes. The objective of this study was to examine the inflammation related effects of 2AA exposure from gestation to postnatal period on dams that ingested 2AA. To examine 2AA effects, pregnant dams were assigned into dose regimens of 2AA. Dams were fed 2AA contaminated diet during the period of gestation and postpartum. The expression of key gene transcripts reported to be important in mediating inflammatory processes was examined via quantitative RT-PCR. Histologic examination of adipose tissue (AT) was also carried out to understand the anatomy of AT due to 2AA exposure during gestation and two weeks postpartum. Examination of the adipose tissue for microscopic changes revealed no alterations between control and low-dose animals. However, AT of the high-dose animals was infiltrated by increased numbers of CD68+mononuclear cells (macrophages) and small numbers of eosinophils and mast cells, consistent with inflammation. In addition, analysis of the mRNA expression of cytokines and adipokines demonstrated the importance of inflammation in AT dysfunction. For instance, TNFα, LEPTIN and IL-6 transcripts were relatively more expressed in the low dose animals than in the high dose and control rats. At the protein level, however, high amounts of cytokines were noted. The effects of 2AA on pregnant dams appear to be more pronounced in the high dose group than in the low dose group, possibly indicating increased susceptibility of rat offspring within this group to elicit a diabetic-type response

  • Toxicity Assessment of Sprague Dawley Offspring Exposed to 2-Aminoanthracene In Utero
    Digital Commons@Georgia Southern, 2016
    Co-Authors: Toure R., Gato, Worlanyo E.
    Abstract:

    Environmental exposures to toxic chemicals increase the risk of developing diseases such as type 2 diabetes. 2-Aminoanthracene (2AA) is an aromatic amine commonly found in the environment including road tars, dyes, and cigarette smoke. Previous studies have found a link between 2AA and insulin-dependent gene expression changes. The goal of the present investigation is to examine the expression of a select pancreatic genes in response to 2AA in utero. Timed Sprague Dawley pregnant rats were fed 0 mg/kg (control), 50 mg/kg (low dose), and 100 mg/kg (high dose) concentrations of 2AA diet. After sacrifice, a histological examination of the pancreas was performed to determine the microscopic anatomy of pancreatic cells followed by specific insulin staining and β-cell mass analysis. Quantification of insulin-dependent transcripts; FTO, GCK, IGF2BP2, PPARG1, GLUT2 and INS1 via qRT-PCR is underway. Insulin IHC staining indicated that pancreatic islet of the low dose progeny had the highest total percentage of pancreatic tissue, followed by the control offspring and then the high dose exposed rats. This experiment is ongoing to examine how these parameters change with moderately high fat diet three months postwean. Also, clinical chemistry of pancreatic enzymes will be undertaken. Quantification of the expression of genes associated with the regulation of insulin and obesity might suggest a link between diabetes and exposure to environmental chemicals such as an aromatic amine

Alessandra Guerrini - One of the best experts on this subject based on the ideXlab platform.

  • chemical characterization gc ms and nmr fingerprinting and bioactivities of south african pelargonium capitatum l l her geraniaceae essential oil
    Chemistry & Biodiversity, 2011
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Massimiliano Tognolini, Carlo Romagnoli, Fabiana Antognoni, Silvia Vertuani, Alessandro Medici, A Bruni
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties.

  • Chemical Composition, NMR Fingerprinting, Mutagenic/antimutagenic and Functional Properties of Commercial South-African Pelargonium capitatum (L.) L’Herit. (Geraniaceae) Essential Oil
    'Wiley', 2011
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Gianni Sacchetti, Tognolini Massimiliano, Ferruccio Poli, Elena Besco, Bruni Renato
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties

  • bioactivities of piper aduncum l and piper obliquum ruiz pavon piperaceae essential oils from eastern ecuador
    Environmental Toxicology and Pharmacology, 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Abstract Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13 C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole-rich P. obliquum (45.8%), while P. aduncum main constituent was dillapiol (45.9%). No genotoxic activity was found in the Ames/ Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500 μg/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacity.

  • Bioactivities of Piper aduncum L. and Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador
    'Elsevier BV', 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole rich P. obliquum(45.8%), while P. aduncum main constituent was dillapiol (45.9%).No genotoxic activity was found in the Ames/Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500g/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacit

Renato Bruni - One of the best experts on this subject based on the ideXlab platform.

  • bioactivities of piper aduncum l and piper obliquum ruiz pavon piperaceae essential oils from eastern ecuador
    Environmental Toxicology and Pharmacology, 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Abstract Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13 C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole-rich P. obliquum (45.8%), while P. aduncum main constituent was dillapiol (45.9%). No genotoxic activity was found in the Ames/ Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500 μg/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacity.

  • Bioactivities of Piper aduncum L. and Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador
    'Elsevier BV', 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole rich P. obliquum(45.8%), while P. aduncum main constituent was dillapiol (45.9%).No genotoxic activity was found in the Ames/Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500g/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacit

Guglielmo Paganetto - One of the best experts on this subject based on the ideXlab platform.

  • chemical characterization gc ms and nmr fingerprinting and bioactivities of south african pelargonium capitatum l l her geraniaceae essential oil
    Chemistry & Biodiversity, 2011
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Massimiliano Tognolini, Carlo Romagnoli, Fabiana Antognoni, Silvia Vertuani, Alessandro Medici, A Bruni
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties.

  • Chemical characterization (GC-MS and NMR fingerprinting) and bioactivities of South-African Pelargonium capitatum (L.) L’Herit. (Geraniaceae) essential oil
    'Wiley', 2011
    Co-Authors: D. Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Massimiliano Tognolini, Carlo Romagnoli, Fabiana Antognoni, Silvia Vertuani, Alessandro Medici, A Bruni
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties

  • Chemical Composition, NMR Fingerprinting, Mutagenic/antimutagenic and Functional Properties of Commercial South-African Pelargonium capitatum (L.) L’Herit. (Geraniaceae) Essential Oil
    'Wiley', 2011
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Gianni Sacchetti, Tognolini Massimiliano, Ferruccio Poli, Elena Besco, Bruni Renato
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties

  • bioactivities of piper aduncum l and piper obliquum ruiz pavon piperaceae essential oils from eastern ecuador
    Environmental Toxicology and Pharmacology, 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Abstract Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13 C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole-rich P. obliquum (45.8%), while P. aduncum main constituent was dillapiol (45.9%). No genotoxic activity was found in the Ames/ Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500 μg/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacity.

  • Bioactivities of Piper aduncum L. and Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador
    'Elsevier BV', 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole rich P. obliquum(45.8%), while P. aduncum main constituent was dillapiol (45.9%).No genotoxic activity was found in the Ames/Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500g/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacit

Mariavittoria Muzzoli - One of the best experts on this subject based on the ideXlab platform.

  • chemical characterization gc ms and nmr fingerprinting and bioactivities of south african pelargonium capitatum l l her geraniaceae essential oil
    Chemistry & Biodiversity, 2011
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Massimiliano Tognolini, Carlo Romagnoli, Fabiana Antognoni, Silvia Vertuani, Alessandro Medici, A Bruni
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties.

  • Chemical characterization (GC-MS and NMR fingerprinting) and bioactivities of South-African Pelargonium capitatum (L.) L’Herit. (Geraniaceae) essential oil
    'Wiley', 2011
    Co-Authors: D. Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Massimiliano Tognolini, Carlo Romagnoli, Fabiana Antognoni, Silvia Vertuani, Alessandro Medici, A Bruni
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties

  • Chemical Composition, NMR Fingerprinting, Mutagenic/antimutagenic and Functional Properties of Commercial South-African Pelargonium capitatum (L.) L’Herit. (Geraniaceae) Essential Oil
    'Wiley', 2011
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Gianni Sacchetti, Tognolini Massimiliano, Ferruccio Poli, Elena Besco, Bruni Renato
    Abstract:

    Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and 13C- and 1H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC50) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN3, 2-nitrofluorene, and 2-Aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties

  • bioactivities of piper aduncum l and piper obliquum ruiz pavon piperaceae essential oils from eastern ecuador
    Environmental Toxicology and Pharmacology, 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Abstract Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13 C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole-rich P. obliquum (45.8%), while P. aduncum main constituent was dillapiol (45.9%). No genotoxic activity was found in the Ames/ Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500 μg/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacity.

  • Bioactivities of Piper aduncum L. and Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador
    'Elsevier BV', 2009
    Co-Authors: Alessandra Guerrini, Damiano Rossi, Guglielmo Paganetto, Mariavittoria Muzzoli, Elisa Andreotti, Massimiliano Tognolini, Maria Elena Maldonado, Gianni Sacchetti, Renato Bruni
    Abstract:

    Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC–FID, GC–MS, 13C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole rich P. obliquum(45.8%), while P. aduncum main constituent was dillapiol (45.9%).No genotoxic activity was found in the Ames/Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-Aminoanthracene as mutagens/promutagens, was observed against promutagen 2-Aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram− bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500g/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacit