3-Hydroxybutyrate

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Terumi Saito - One of the best experts on this subject based on the ideXlab platform.

  • Purification and molecular cloning of an intracellular 3-Hydroxybutyrate-oligomer hydrolase from Paucimonas lemoignei.
    Journal of bioscience and bioengineering, 2007
    Co-Authors: Keiichi Uchino, Yoko Katsumata, Tomoko Takeda, Hiroki Arai, Mari Shiraki, Terumi Saito
    Abstract:

    An intracellular 3-Hydroxybutyrate-oligomer hydrolase was purified from a poly(3-Hydroxybutyrate)-degrading bacterium, Paucimonas lemoignei. It hydrolyzed the 3-Hydroxybutyrate dimer with the highest specific activity of any of the enzymes reported so far. The gene was cloned and sequenced. The deduced amino acid sequence showed that the enzyme is a homolog of the PhaZc of Ralstonia eutropha H16.

  • Novel Intracellular 3-Hydroxybutyrate-Oligomer Hydrolase in Wautersia eutropha H16
    Journal of bacteriology, 2005
    Co-Authors: Teruyuki Kobayashi, Keiichi Uchino, Tomoko Abe, Yuya Yamazaki, Terumi Saito
    Abstract:

    Wautersia eutropha H16 (formerly Ralstonia eutropha) mobilizes intracellularly accumulated poly(3-Hydroxybutyrate) (PHB) with intracellular poly(3-Hydroxybutyrate) depolymerases. In this study, a novel intracellular 3-Hydroxybutyrate-oligomer hydrolase (PhaZc) gene was cloned and overexpressed in Escherichia coli. Then PhaZc was purified and characterized. Immunoblot analysis with polyclonal antiserum against PhaZc revealed that most PhaZc is present in the cytosolic fraction and a small amount is present in the poly(3-Hydroxybutyrate) inclusion bodies of W. eutropha. PhaZc degraded various 3-Hydroxybutyrate oligomers at a high specific activity and artificial amorphous poly(3-Hydroxybutyrate) at a lower specific activity. Native PHB granules and semicrystalline PHB were not degraded by PhaZc. A PhaZ deletion mutation enhanced the deposition of PHB in the logarithmic phase in nutrient-rich medium. PhaZc differs from the hydrolases of W. eutropha previously reported and is a novel type of intracellular 3-Hydroxybutyrate-oligomer hydrolase, and it participates in the mobilization of PHB along with other hydrolases.

  • Biopolymers Online - Intracellular Degradation of Polyhydroxyalkanoates (PHAs)
    Biopolymers Online, 2002
    Co-Authors: Terumi Saito, Teruyuki Kobayashi
    Abstract:

    Introduction Historical Outline Intracellular PHA Depolymerase Endogenous Degradation of PHA Intracellular mcl-PHA Depolymerase Intracellular Poly(3HB) Depolymerase Intracellular d(–)-3-Hydroxybutyrate Oligomer Hydrolase Other Enzymes Related to PHA Degradative Metabolism d(–)-3-Hydroxybutyrate Dehydrogenase Acetoacetyl-CoA Transferase and Acetoacetyl-CoA Synthetase 3-Ketothiolase Outlook and Perspectives Patents Keywords: intracellular poly(3HB) depolymerase; intracellular PHA depolymerase; poly(3HB) depolymerase; 3-Hydroxybutyrate dehydrogenase; phaZ; succinyl-CoA transferase; acetoacetyl-CoA synthetase; 3-Hydroxybutyrate oligomer hydrolase; 3-Hydroxybutyrate dimer hydrolase; 3-ketothiolase; Ralstonia eutropha; Azotobacter beijerinkii; Zoogloea ramigera; Legionella pneumophila; Pseudomonas oleovorans; Pseudomonas aeruginosa; Hydrogenophaga pseudoflava; Rhodospirillum rubrum; Bacillus megaterium.

  • Radiation-induced degradation of poly(3-Hydroxybutyrate) and the copolymer poly(3-Hydroxybutyrate-co-3-hydroxyvalerate)
    Polymer Degradation and Stability, 1994
    Co-Authors: Hiroshi Mitomo, Yuhei Watanabe, Isao Ishigaki, Terumi Saito
    Abstract:

    Poly(3-Hydroxybutyrate) {P(3HB)} and the copolymer poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) {P(3HB-co-3HV)} were irradiated with γ-rays at 25°C in air and in vacuum. Melting points (Tm) and glass-transition temperatures (Tg) were measured by differential scanning calorimetry. Number-average molecular weights (Mn) were analyzed by gel permeation chromatography. No significant differences were observed between Tm values of P(3HB) and P(3HB-co-3HV) irradiated in air and in vacuum, which decreased almost linearly with increasing irradiation dose. The Mn values of both samples decreased sharply with increasing dose, reflecting typical random chain scission. The decrease in Mn of the sample irradiated in vacuum was smaller than that irradiated in air with the same dose, implying the occurrence of crosslinking. The Tg values for both polymers irradiated in vacuum remained almost unchanged over a wide dose range, while those irradiated in air decreased as the irradiation dose increased. Both the Tm and Tg of samples irradiated in air were inversely proportional to Mn. Biodegradability was clearly promoted with decreasing molecular weight.

  • In vivo and in vitro degradation of poly(3-Hydroxybutyrate) in pat
    Biomaterials, 1991
    Co-Authors: Terumi Saito, Kenkichi Tomita, Kazuhiko Juni, Kenkichi Ooba
    Abstract:

    The inflammatory activity and biodegradation of poly(3-Hydroxybutyrate) were examined. Poly(3-Hydroxybutyrate) sheet did not cause any inflammation in the chorioallantoic membrane of the developing egg. The i.v. injection of 14C-labelled poly(3-Hydroxybutyrate) granules showed that 86, 2.5 and 2.4% of the total radioactivity administered were distributed in the liver, spleen and lung, respectively, and the radioactivity decreased slowly but steadily in most tissues examined during 2 month. Crude extracts of rat tissues showed that the activity degraded the poly(3-Hydroxybutyrate) granules in vitro.

Guo-qiang Chen - One of the best experts on this subject based on the ideXlab platform.

  • Microbial production of 4-hydroxybutyrate, poly-4-hydroxybutyrate, and poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) by recombinant microorganisms
    Applied microbiology and biotechnology, 2009
    Co-Authors: Lei Zhang, Zhen-yu Shi, Guo-qiang Chen
    Abstract:

    4-Hydroxybutyrate (4HB) was produced by Aeromonas hydrophila 4AK4, Escherichia coli S17-1, or Pseudomonas putida KT2442 harboring 1,3-propanediol dehydrogenase gene dhaT and aldehyde dehydrogenase gene aldD from P. putida KT2442 which are capable of transforming 1,4-butanediol (1,4-BD) to 4HB. 4HB containing fermentation broth was used for production of homopolymer poly-4-hydroxybutyrate [P(4HB)] and copolymers poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-4HB)]. Recombinant A. hydrophila 4AK4 harboring plasmid pZL-dhaT-aldD containing dhaT and aldD was the most effective 4HB producer, achieving approximately 4 g/l 4HB from 10 g/l 1,4-BD after 48 h of incubation. The strain produced over 10 g/l 4HB from 20 g/l 1,4-BD after 52 h of cultivation in a 6-L fermenter. Recombinant E. coli S17-1 grown on 4HB containing fermentation broth was found to accumulate 83 wt.% of intracellular P(4HB) in shake flask study. Recombinant Ralstonia eutropha H16 grew to over 6 g/l cell dry weight containing 49 wt.% P(3HB-13%4HB) after 72 h.

  • Medical Application of Microbial Biopolyesters Polyhydroxyalkanoates
    Artificial cells blood substitutes and immobilization biotechnology, 2009
    Co-Authors: Yang Wang, Guo-qiang Chen
    Abstract:

    Polyhydroxyalkanoates (PHA) are a family of polyesters synthesized by microorganisms under unbalanced growth conditions. PHA including poly-3-Hydroxybutyrate (PHB), copolymers of 3-Hydroxybutyrate and 3-hydroxyvalerate (PHBV), poly-4-hydroxybutyrate (P4HB), copolymers of 3-Hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and poly-3-hydroxyoctanoate (PHO) are now available in sufficient quantity for tissue engineering medical application studies due to their reasonable biocompatibility, adjustable mechanical properties, and controllable biodegradability. This paper reviews many achievements based on PHA for medical devices development, tissue repair, artificial organ construction, drug delivery, and nutritional/therapeutic uses. Combined with the recent FDA approval for P4HB clinical application, one can expect a good prospect for PHA application in the medical fields.

  • Physical properties and biocompatibility of poly(3-Hydroxybutyrate-co-3-hydroxyhexanoate) blended with poly(3-Hydroxybutyrate-co-4-hydroxybutyrate).
    Journal of biomaterials science. Polymer edition, 2009
    Co-Authors: Ling Luo, Xing Wei, Guo-qiang Chen
    Abstract:

    Poly(3-Hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) was blended with poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) (P3HB4HB) to improve physical properties and biocompatibility of PHBHHx for a wide range of biomedical applications. PHBHHx was completely miscible with P3HB4HB in their blends. All the PHBHHx/P3HB4HB blends showed improved physical properties compared with PHBHHx, including higher thermal stability, flexibility and mechanical strength. All the blends had more hydrophilic surface, higher polar component and rougher surface than PHBHHx. The PHBHHx/P3HB4HB blend in 4:2 weight ratio showed the roughest surface and also had the highest chondrocyte viability among all the blends and the polymers tested, which was 59% higher than that on PHBHHx and 32% higher than that on P3HB4HB. The blend with 4:2 weight ratio also had the maximum cartilage-specific collagen II mRNA expression among all the blends and the polymers tested, which was 9-times higher than that on PHBHHx and 8-times higher than that on P3HB4HB. These results demonstrated that PHBHHx had improved physical properties and biocompatibility after blending with P3HB4HB. The blends could be used for cartilage tissue engineering.

  • nanofibrous polyhydroxyalkanoate matrices as cell growth supporting materials
    Biomaterials, 2008
    Co-Authors: Yan Zhang, Guo-qiang Chen
    Abstract:

    Polyhydroxyalkanoates (PHAs) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. To mimic the real microenvironment of extracellular matrix (ECM) for cell growth, novel nanofiber matrices based on PHA polymers were prepared via a phase separation process. Three-dimensional interconnected fibrous networks were observed in these matrices with average fiber diameters of 50–500 nm, which are very similar to the major ECM component collagen. Compared with nanofiber matrix made of poly(L-lactide), the mechanical properties of PHA nanofiber matrices were significantly improved, especially those matrices of PHA blends PHB/PHBHHx containing polyhydroxybutyrate (PHB) and copolyesters PHBHHx consisting of 3-Hydroxybutyrate and 3-hydroxyhexanoate, and PHB/P3HB4HB that are PHB blended with copolyesters of 3-Hydroxybutyrate and 4hydroxybutyrate, respectively. More importantly, cell attachment and growth of human keratinocyte cell line HaCat on the nanofiber PHA matrices showed a notable improvement over those on PHA matrices prepared via an ordinary solution casting method. It was therefore proposed that PHA nanofiber matrices combined the advantages of biodegradation, improved mechanical strengths and the nanostructure of a natural extracellular matrix, leading to a better cell compatibility, thus they can be used for future implant biomaterial development. 2008 Published by Elsevier Ltd.

  • Thermal analyses of poly(3-Hydroxybutyrate), poly(3-Hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-Hydroxybutyrate-co-3-hydroxyhexanoate)
    Journal of Applied Polymer Science, 2001
    Co-Authors: Man Ken Cheung, Guo-qiang Chen
    Abstract:

    Thermal analyses of poly(3-Hydroxybutyrate) (PHB), poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) [P(HB–HV)], and poly(3-Hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB–HHx)] were made with thermogravimetry and differential scanning calorimetry (DSC). In the thermal degradation of PHB, the onset of weight loss occurred at the temperature (°C) given by To = 0.75B + 311, where B represents the heating rate (°C/min). The temperature at which the weight-loss rate was at a maximum was Tp = 0.91B + 320, and the temperature at which degradation was completed was Tf = 1.00B + 325. In the thermal degradation of P(HB–HV) (70:30), To = 0.96B + 308, Tp = 0.99B + 320, and Tf = 1.09B + 325. In the thermal degradation of P(HB–HHx) (85:15), To = 1.11B + 305, Tp = 1.10B + 319, and Tf = 1.16B + 325. The derivative thermogravimetry curves of PHB, P(HB–HV), and P(HB–HHx) confirmed only one weight-loss step change. The incorporation of 30 mol % 3-hydroxyvalerate (HV) and 15 mol % 3-hydroxyhexanoate (HHx) components into the polyester increased the various thermal temperatures To, Tp, and Tf relative to those of PHB by 3–12°C (measured at B = 40°C/min). DSC measurements showed that the incorporation of HV and HHx decreased the melting temperature relative to that of PHB by 70°C. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 82: 90–98, 2001

Henry E Valentin - One of the best experts on this subject based on the ideXlab platform.

  • Production of poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose.
    Journal of biotechnology, 1997
    Co-Authors: Henry E Valentin, Douglas E. Dennis
    Abstract:

    A recombinant Escherichia coli strain has been developed that produces poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) when grown in complex medium containing glucose. This has been accomplished by introducing into E. coli DH5 alpha separate plasmids harboring the polyhydroxyalkanoate (PHA) biosynthesis genes from Ralstonia eutropha (formerly named Alcaligenes eutrophus) and the succinate degradation genes from Clostridium kluyveri, respectively. Poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) levels reached 50% of the cell dry weight and contained up to 2.8 mol.% 4-hydroxybutyrate. The molecular weight of the polymer was 1.8 x 10(6).

  • Metabolic Pathway for Biosynthesis of Poly (3‐Hydroxybutyrate‐co‐4‐Hydroxybutyrate) from 4‐Hydroxybutyrate by Alcaligenes eutrophus
    European journal of biochemistry, 1995
    Co-Authors: Henry E Valentin, Gundula Zwingmann, Andreas Schonebaum, Alexander Steinbuchel
    Abstract:

    Various aerobic Gram-negative bacteria have been examined for their ability to use 4-hydroxybutyrate and 1,4-butanediol as carbon source for growth. Alcaligenes eutrophus strains H16, HF39, PHB−4 and Pseudomonas denitrificans‘Morris’ were not able to grow with 1,4-butanediol or 4-hydroxybutyrate. From A. eutrophus HF39 spontaneous primary mutants (e. g. SK4040) were isolated which grew on 4-hydroxybutyrate with doubling times of approximately 3 h. Tn5::mob mutagenesis of mutant SK4040 led to the isolation of two phenotypically different classes of secondary mutants which were affected in the utilization of 4-hydroxybutyrate. Mutants exhibiting the phenotype 4-hydroxybutyrate-negative did not grow with 4-hydroxybutyrate, and mutants exhibiting the phenotype 4-hydroxybutyrate-leaky grew at a significantly lower rate with 4-hydroxybutyrate. Hybridization experiments led to the identification of a 10-kbp genomic EcoRI fragment of A. eutrophus SK4040, which was altered in mutants with the phenotype 4-hydroxybutyrate-negative, and of two 1-kbp and 4.5-kbp genomic EcoRI fragments, which were altered in mutants with the phenotype 4-hydroxybutyrate-leaky. This 10-kbp EcoRI fragment was cloned from A. eutrophus SK4040, and conjugative transfer of a pVDZ'2 hybrid plasmid to A. eutrophus H16 conferred the ability to grow with 4-hydroxybutyrate to the wild type. DNA-sequence analysis of this fragment, enzymic analysis of the wild type and of mutants of A. eutrophus as well as of recombinant strains of Escherichia coli led to the identification of a structural gene encoding for a 4-hydroxybutyrate dehydrogenase which was affected by transposon mutagenesis in five of six available 4-hydroxybutyrate-negative mutants. Enzymic studies also provided evidence for the presence of an active succinate-semialdehyde dehydrogenase in 4-hydroxybutyrate-grown cells. This indicated that degradation of 4-hydroxybutyrate occurs via succinate semialdehyde and succinate and that the latter is degraded by the citric acid cycle. NMR studies of poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) accumulated from 4-hydroxy [1-13C]butyrate or 4-hydroxy[2-13C]butyrate as substrate gave no evidence for a direct conversion of 4-hydroxybutyrate into 3-Hydroxybutyrate and therefore supported the results of enzymic analysis.

  • metabolic pathway for biosynthesis of poly 3 hydroxybutyrate co 4 hydroxybutyrate from 4 hydroxybutyrate by alcaligenes eutrophus
    FEBS Journal, 1995
    Co-Authors: Henry E Valentin, Gundula Zwingmann, Andreas Schonebaum, Alexander Steinbuchel
    Abstract:

    Various aerobic Gram-negative bacteria have been examined for their ability to use 4-hydroxybutyrate and 1,4-butanediol as carbon source for growth. Alcaligenes eutrophus strains H16, HF39, PHB−4 and Pseudomonas denitrificans‘Morris’ were not able to grow with 1,4-butanediol or 4-hydroxybutyrate. From A. eutrophus HF39 spontaneous primary mutants (e. g. SK4040) were isolated which grew on 4-hydroxybutyrate with doubling times of approximately 3 h. Tn5::mob mutagenesis of mutant SK4040 led to the isolation of two phenotypically different classes of secondary mutants which were affected in the utilization of 4-hydroxybutyrate. Mutants exhibiting the phenotype 4-hydroxybutyrate-negative did not grow with 4-hydroxybutyrate, and mutants exhibiting the phenotype 4-hydroxybutyrate-leaky grew at a significantly lower rate with 4-hydroxybutyrate. Hybridization experiments led to the identification of a 10-kbp genomic EcoRI fragment of A. eutrophus SK4040, which was altered in mutants with the phenotype 4-hydroxybutyrate-negative, and of two 1-kbp and 4.5-kbp genomic EcoRI fragments, which were altered in mutants with the phenotype 4-hydroxybutyrate-leaky. This 10-kbp EcoRI fragment was cloned from A. eutrophus SK4040, and conjugative transfer of a pVDZ'2 hybrid plasmid to A. eutrophus H16 conferred the ability to grow with 4-hydroxybutyrate to the wild type. DNA-sequence analysis of this fragment, enzymic analysis of the wild type and of mutants of A. eutrophus as well as of recombinant strains of Escherichia coli led to the identification of a structural gene encoding for a 4-hydroxybutyrate dehydrogenase which was affected by transposon mutagenesis in five of six available 4-hydroxybutyrate-negative mutants. Enzymic studies also provided evidence for the presence of an active succinate-semialdehyde dehydrogenase in 4-hydroxybutyrate-grown cells. This indicated that degradation of 4-hydroxybutyrate occurs via succinate semialdehyde and succinate and that the latter is degraded by the citric acid cycle. NMR studies of poly(3-Hydroxybutyrate-co-4-hydroxybutyrate) accumulated from 4-hydroxy [1-13C]butyrate or 4-hydroxy[2-13C]butyrate as substrate gave no evidence for a direct conversion of 4-hydroxybutyrate into 3-Hydroxybutyrate and therefore supported the results of enzymic analysis.

Nobuya Itoh - One of the best experts on this subject based on the ideXlab platform.

  • Biocatalytic production of (S)-4-bromo-3-Hydroxybutyrate and structurally related chemicals and their applications
    Applied Microbiology and Biotechnology, 2009
    Co-Authors: Hiroyuki Asako, Masatoshi Shimizu, Nobuya Itoh
    Abstract:

    The enzymatic production of ( S )-4-bromo-3-Hydroxybutyrate has been poorly studied compared with ( S )-4-chloro-3-Hydroxybutyrate. This can be attributed to the toxicity of bromide for biocatalysis. Recently, we isolated cDNA that encodes Penicillium citrinum β-keto ester reductase (KER) and the gene that encodes Leifsonia sp. alcohol dehydrogenase, which catalyzes the reduction of methyl 4-bromo-3-oxobutyrate to methyl ( S )-4-bromo-3-Hydroxybutyrate with high optical purity and productivity and expressed them in Escherichia coli . Moreover, protein engineering was performed using error-prone PCR-based random mutagenesis to improve the thermostability and enantioselectivity of KER. This review focuses on the establishment of a novel biotechnological process for the production of ( S )-4-bromo-3-Hydroxybutyrate using E. coli transformants. This process is suitable for industrial production of ( S )-4-bromo-3-Hydroxybutyrate, an intermediate for statin compounds.

Luiz A. F. Coelho - One of the best experts on this subject based on the ideXlab platform.

  • Phase behavior of poly(3-Hydroxybutyrate)/poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) blends
    Fluid Phase Equilibria, 2007
    Co-Authors: Denise S. Conti, Maria Irene Yoshida, Sérgio Henrique Pezzin, Luiz A. F. Coelho
    Abstract:

    Miscibility, molecular interactions and crystallinity of blends of poly(3-Hydroxybutyrate) [P(3HB)] with poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] were studied in this work. P(3HB)/P(3HB-co-3HV)-6%3HV blends were prepared by casting and characterized by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). The results for the glass transition temperatures (T g ) and the melting temperatures (T m ) showed that most of the blends are miscible in amorphous and melting phases. A good agreement between values of experimental T g S for miscible blends and those predicted by Fox equation was achieved. XRD analyses confirmed the crystallinity degrees found by DSC. Measurement of T m depression for the blends allowed to determine the Flory-Huggins interaction parameter (Χ 12 ) using the Nishi-Wang equation. The value of Χ 12 obtained is negative, indicating miscibility of the components of the blends, which is in agreement with DSC experiments.

  • Mechanical and Morphological Properties of Poly(3‐hydroxybutyrate)/Poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) Blends
    Macromolecular Symposia, 2006
    Co-Authors: Denise S. Conti, Sérgio Henrique Pezzin, Luiz A. F. Coelho
    Abstract:

    With the objective of developing new biodegradable materials, the mechanical properties and the morphology of blends of poly(3-Hydroxybutyrate), P(3HB), and poly(3-Hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), were studied in this work. P(3HB) (492 kg · mol−1)/P(3HB-co-3HV)-6%3HV (294.2 kg · mol−1) blends were prepared by injection in a wide range of proportions and characterized by mechanical behavior of tensile strength, Izod impact strength and hardness Shore D. According to the increase of the copolymer content in the blend, it was detected that the hardness Shore D and the maximum tensile strength presented a significant reduction, the elasticity modulus showed a significant reduction, the elongation at break presented a significant increase and the Izod impact strength practically remained constant. Scanning electronic microscopy (SEM) was carried out in the fractured surface of the samples obtained during the tests of tensile and impact strength. These analyses showed a morphology with fragile fracture for whole blends, agreeing with mechanical results previously reported.

  • Miscibility and crystallinity of poly(3-Hydroxybutyrate)/poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) blends
    Thermochimica Acta, 2006
    Co-Authors: Denise S. Conti, Maria Irene Yoshida, Sérgio Henrique Pezzin, Luiz A. F. Coelho
    Abstract:

    Abstract With the objective of developing new biodegradable materials, the miscibility and the crystallinity of blends of poly(3-Hydroxybutyrate), P(3HB), and poly(3-Hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), have been studied. P(3HB) (300 kg mol−1)/P(3HB-co-3HV)–10% 3HV (340 kg mol−1) blends were prepared by casting in a wide range of proportions, and characterized by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR). The experimental values for the glass transition temperatures (Tg) are in good agreement with the values provided by the Fox equation, showing that the blends are miscible. It was observed that the Tg and the melting temperature (Tm) decreases with the increase in the P(3HB-co-3HV)–10% 3HV content, while the crystallization temperature (Tc) increases. FT-IR analyses confirmed the decrease on the crystallinity of P(3HB)/P(3HB-co-3HV)–10% 3HV blends with higher copolymer contents. Bands related to the crystallinity were changed, due to the copolymer content that produced miscible and less crystalline blends.