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Sunghoon Park - One of the best experts on this subject based on the ideXlab platform.
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a novel 3 hydroxypropionic Acid inducible promoter regulated by the lysr type transcriptional activator protein mmsr of pseudomonas denitrificans
Scientific Reports, 2019Co-Authors: Nam Hoai Nguyen, Sunghoon Park, Satish Kumar Ainala, Shengfang ZhouAbstract:MmsR (33.3 kDa) is a putative LysR-type transcriptional activator of Pseudomonas denitrificans. With the help of 3-Hydroxypropionic Acid (3-HP), an important platform chemical, MmsR positively regulates the expression of mmsA, which encodes methylmalonylsemialdehyde dehydrogenase, the enzyme involved in valine degradation. In the present study, the cellular function of MmsR and its binding to the regulatory DNA sequence of mmsA expression were investigated both in vivo and in vitro. Transcription of the mmsA was enhanced >140-fold in the presence of 3-HP. In the MmsR-responsive promoter region, two operators showing dyad symmetry, designated O1 and O2 and centered at the −79 and −28 positions, respectively, were present upstream of the mmsA transcription start site. An electrophoretic mobility shift assay indicated that MmsR binds to both operator sites for transcription activation, probably in cooperative manner. When either O1 or O2 or both regions were mutated, the inducibility by the MmsR-3-HP complex was significantly reduced or completely removed, indicating that both sites are required for transcription activation. A 3-HP sensor was developed by connecting the activation of MmsR to a green fluorescent readout. A more than 50-fold induction by 25 mM 3-HP was observed.
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Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor
Biotechnology and Bioprocess Engineering, 2018Co-Authors: Nam Hoai Nguyen, Jung-rae Kim, Sunghoon ParkAbstract:Application of the recently developed wholecell 3-HP biosensor, which detects 3-Hydroxypropionic Acid (3-HP) and expresses fluorescence as an output signal in response to the 3-HP concentration, was studied in three areas of enzyme and metabolic engineering. First, a sensor was applied to identify active 3-hydroxyaldehyde dehydrogenase (ALDH), a key enzyme in the 3-HP production pathway. Second, with the aid of helper enzymes that catalyze the conversion of 1,3-propanediol (1,3-PDO) to 3-HP, a 3-HP biosensor was converted into a 1,3-PDO biosensor. Third, a 3-HP biosensor, with proper modifications in expression module of the output signal and the use of pH-tolerant red fluorescent protein (RFP), was shown to monitor the rate of 3-HP production under process conditions in which one or more interfering compounds are present in the culture medium and/or the medium pH decreases. This study demonstrates that 3-HP biosensors can be widely used in enzyme and metabolic engineering applications for 3-HP production.
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potential and limitations of klebsiella pneumoniae as a microbial cell factory utilizing glycerol as the carbon source
Biotechnology Advances, 2017Co-Authors: Vinod Kumar, Sunghoon ParkAbstract:Klebsiella pneumoniae is a Gram-negative facultative anaerobe that metabolizes glycerol efficiently under both aerobic and anaerobic conditions. This microbe is considered an outstanding biocatalyst for transforming glycerol into a variety of value-added products. Crude glycerol is a cheap carbon source and can be converted by K. pneumoniae into useful compounds such as lactic Acid, 3-Hydroxypropionic Acid, ethanol, 1,3-propanediol, 2,3-butanediol, and succinic Acid. This review summarizes glycerol metabolism in K. pneumoniae and its potential as a microbial cell factory for the production of commercially important Acids and alcohols. Although many challenges remain, K. pneumoniae is a promising workhorse when glycerol is used as the carbon source.
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Anodic electro-fermentation of 3-Hydroxypropionic Acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system
'Springer Science and Business Media LLC', 2017Co-Authors: Changman Kim, Sunghoon Park, Mi Yeon Kim, Iain Michie, Byong-hun Jeon, Giuliano C. Premier, Jung-rae KimAbstract:Abstract Background 3-Hydroxypropionic Acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes
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Deletion of putative oxidoreductases from Klebsiella pneumoniae J2B could reduce 1,3-propanediol during the production of 3-Hydroxypropionic Acid from glycerol
Biotechnology and Bioprocess Engineering, 2015Co-Authors: Somasundar Ashok, Satish Kumar Ainala, Eunhee Seol, Sunghoon ParkAbstract:Recombinant Klebsiella pneumoniae over-expressing 3-hydroxypropionaldehyde (3-HPA) dehydrogenase can produce 3-Hydroxypropionic Acid (3-HP), an important platform chemical, from glycerol. However, K. pneumoniae co-produces 1,3-propanediol (1,3-PDO) due to the presence of 1,3-propanediol oxidoreductases, which decreases the titer and yield of 3-HP. Previously, two major oxidoreductases, dhaT and yqhD , were removed from K. pneumoniae ; however the mutant still produced a significant amount of 1,3-PDO, indicating the probable existence of other oxidoreductase(s). Genome analysis of K. pneumoniae revealed the presence of five putative oxidoreductases having high amino Acid similarities to both DhaT (primary 1,3-propanediol oxidoreductase) and YqhD (aldehyde dehydrogenase). Among them, adhE was highly expressed in the absence of DhaT and YqhD. Additionally, an alkyl hydroperoxide oxidoreductase ( ahpF ), albeit dissimilar to both DhaT and YqhD, was highly expressed in the absence of DhaT and YqhD. To examine the role of adhE and ahpF in 1,3-PDO production, mutant strains devoid of dhaT , yqhD , ahpF and/or adhE genes were developed. However, these mutants neither reduced the production of 1,3-PDO nor improved the production of 3-HP when engineered to over-express an aldehyde dehydrogenase (KGSADH). These results indicate that, apart from DhaT, YqhD, AhpF and AdhE, K. pneumoniae has other, unknown oxidoreductases that are involved in 1,3-PDO production. It is concluded that complete elimination of 1,3-PDO during 3-HP production from glycerol by K. pneumoniae is highly challenging.
Florent Allais - One of the best experts on this subject based on the ideXlab platform.
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New insights in reactive extraction mechanisms of organic Acids: An experimental approach for 3-Hydroxypropionic Acid extraction with tri-n-octylamine
Separation and Purification Technology, 2017Co-Authors: Florian Chemarin, Marwen Moussa, Florent Allais, Morad Chadni, Brigitte Pollet, Pascale Lieben, Ioan-cristian Trelea, Violaine Athes-dutourAbstract:A detailed study of 3-Hydroxypropionic Acid (3-HP) reactive extraction with tri-n-octylamine (TOA) is proposed for the first time. It aims at uncovering some solvent-solutes interactions and providing global mechanisms to better understand and design the reactive liquid-liquid extraction of 3-HP in a biotechnological process. Eleven solvents of similar molecular sizes and several chemical types (alcohols, esters and alkanes) were investigated to understand their role on the extraction ability. Alcohols were found to be the best solvents thanks to their H-bond donor characteristic and water loading that allowed good solvation of the Acid-amine complexes. Further investigations were then undertaken, for n-decanol and oleyl alcohol as solvents, varying Acid (0.0028-0.56 mol/L corresponding to 0.25-50 g/L) and amine (02,3 corresponding to 0-100% v/v) concentrations. At 0.011 mol/L (1 g/L) of 3-HP, maximum extraction yields of 77% for n-decanol and 51% for oleyl alcohol were found for 0.46 mol/L TOA (20% v/v). The initial TOA purity proved to have a major impact on the extraction yield at low initial Acid concentration (
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Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938
Fermentation, 2017Co-Authors: Julien Couvreur, Florent Allais, Andreia Teixeira, Henry-eric Spinnler, Claire Saulou-bérion, Tiphaine ClementAbstract:An experimental design based on Response Surface Methodology (RSM) was used for the formulation of a growth medium based on sugar beet and wheat processing coproducts adapted to the cultivation of Lactobacillus reuteri (L. reuteri) DSM17938. The strain was cultivated on 30 different media varying by the proportions of sugar beet and wheat processing coproducts, and the concentration of yeast extract, tween 80 and vitamin B12. The media were used in a two-step process consisting of L. reuteri cultivation followed by the bioconversion of glycerol into 3-Hydroxypropionic Acid by resting cells. The efficiency of the formulations was evaluated according to the maximal optical density at the end of the growth phase (∆OD 620nm) and the ability of the resting cells to convert glycerol into 3-Hydroxypropionic Acid, a platform molecule of interest for the plastic industry. De Man, Rogosa, and Sharpe medium (MRS), commonly used for the cultivation of lactic bacteria, was used as the control medium. The optimized formulation allowed increasing the 3-HP production.
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reactive extraction of bio based 3 hydroxypropionic Acid assisted by hollow fiber membrane contactor using toa and aliquat 336 in n decanol
Journal of Chemical Technology & Biotechnology, 2016Co-Authors: Gregoire Burge, Florian Chemarin, Marwen Moussa, Claire Saulouberion, Florent AllaisAbstract:BACKGROUND Reactive liquid–liquid extraction is a promising technique for the direct recovery of carboxylic Acids from bioconversion media. This work focused on the optimization of 3-Hydroxypropionic Acid (3-HP) reactive extraction assisted by hollow-fiber membrane contactor (HFMC), using tri-n-octylamine (TOA) and Aliquat 336 as extractants in n-decanol, and on its practical application for the recovery of 3-HP obtained from glycerol bioconversion by Lactobacillus reuteri. RESULTS The results underlined the influence of the initial pH and 3-HP concentration of the aqueous medium and the impact of extractant phase composition on the extraction performances. The highest distribution coefficient of 3-HP (KD = 13.0) was observed at pH = 3 with 10% v/v TOA mixed with 10% v/v Aliquat 336 in n-decanol. This study showed that the Acid concentration and the ratio of amines used between TOA and Aliquat 336 in n-decanol did not have a great impact on the KD during 3-HP reactive extraction, whereas it had an influence on the extraction kinetics. Moreover, 3-HP reactive extraction assisted by HFMC from real bioconversion broth with glycerol as the substrate was shown to be feasible, with the same order of magnitude of extraction kinetics but with a lower KD compared with reactive extraction using model broth. CONCLUSIONS 3-HP was predominantly extracted from the bioconversion broth, confirming the high selectivity of the extraction process studied and the high potential of this approach applied to the biotechnological production of 3-HP. © 2016 Society of Chemical Industry
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Reactive extraction of bio-based 3-Hydroxypropionic Acid assisted by hollow-fiber membrane contactor using TOA and Aliquat 336 in n-decanol
Journal of Chemical Technology and Biotechnology, 2016Co-Authors: Gregoire Burge, Florian Chemarin, Marwen Moussa, Florent Allais, Henry-eric Spinnler, Claire Saulou-bérion, Violaine Athes-dutourAbstract:BACKGROUND: Reactive liquid-liquid extraction is a promising technique for the direct recovery of carboxylic Acids from bioconversion media. This work focused on the optimization of 3-Hydroxypropionic Acid (3-HP) reactive extraction assisted by hollow-fiber membrane contactor (HFMC), using tri-n-octylamine (TOA) and Aliquat 336 as extractants in n-decanol, and on its practical application for the recovery of 3-HP obtained from glycerol bioconversion by Lactobacillus reuteri. RESULTS: The results underlined the influence of the initial pH and 3-HP concentration of the aqueous medium and the impact of extractant phase composition on the extraction performances. The highest distribution coefficient of 3-HP (K-D = 13.0) was observed at pH = 3 with 10% v/v TOA mixed with 10% v/v Aliquat 336 in n-decanol. This study showed that the Acid concentration and the ratio of amines used between TOA and Aliquat 336 in n-decanol did not have a great impact on the K-D during 3-HP reactive extraction, whereas it had an influence on the extraction kinetics. Moreover, 3-HP reactive extraction assisted by HFMC from real bioconversion broth with glycerol as the substrate was shown to be feasible, with the same order of magnitude of extraction kinetics but with a lower K-D compared with reactive extraction using model broth. CONCLUSIONS: 3-HP was predominantly extracted from the bioconversion broth, confirming the high selectivity of the extraction process studied and the high potential of this approach applied to the biotechnological production of 3-HP.
Jürgen G. Okun - One of the best experts on this subject based on the ideXlab platform.
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simultaneous determination of 3 hydroxypropionic Acid methylmalonic Acid and methylcitric Acid in dried blood spots second tier lc ms ms assay for newborn screening of propionic Acidemia methylmalonic Acidemias and combined remethylation disorders
PLOS ONE, 2017Co-Authors: Péter Monostori, Glynis Klinke, Sylvia Richter, Ákos Baráth, Ralph Fingerhut, Matthias R. Baumgartner, Stefan Kölker, Georg F. Hoffmann, Gwendolyn Gramer, Jürgen G. OkunAbstract:BACKGROUND AND AIMS Increased propionylcarnitine levels in newborn screening are indicative for a group of potentially severe disorders including propionic Acidemia (PA), methylmalonic Acidemias and combined remethylation disorders (MMACBL). This alteration is relatively non-specific, resulting in the necessity of confirmation and differential diagnosis in subsequent tests. Thus, we aimed to develop a multiplex approach for concurrent determination of 3-Hydroxypropionic Acid, methylmalonic Acid and methylcitric Acid from the same dried blood spot (DBS) as in primary screening (second-tier test). We also set out to validate the method using newborn and follow-up samples of patients with confirmed PA or MMACBL. METHODS The assay was developed using liquid chromatography-tandem mass spectrometry and clinically validated with retrospective analysis of DBS samples from PA or MMACBL patients. RESULTS Reliable determination of all three analytes in DBSs was achieved following simple and fast (<20 min) sample preparation without laborious derivatization or any additional pipetting steps. The method clearly distinguished the pathological and normal samples and differentiated between PA and MMACBL in all stored newborn specimens. Methylcitric Acid was elevated in all PA samples; 3-Hydroxypropionic Acid was also high in most cases. Methylmalonic Acid was increased in all MMACBL specimens; mostly together with methylcitric Acid. CONCLUSIONS A liquid chromatography-tandem mass spectrometry assay allowing simultaneous determination of the biomarkers 3-Hydroxypropionic Acid, methylmalonic Acid and methylcitric Acid in DBSs has been developed. The assay can use the same specimen as in primary screening (second-tier test) which may reduce the need for repeated blood sampling. The presented preliminary findings suggest that this method can reliably differentiate patients with PA and MMACBL in newborn screening. The validated assay is being evaluated prospectively in a pilot project for extension of the German newborn screening panel (‟Newborn screening 2020"; Newborn Screening Center, University Hospital Heidelberg).
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LC-MS/MS chromatogram of DBS from a PA patient.
2017Co-Authors: Péter Monostori, Glynis Klinke, Sylvia Richter, Ákos Baráth, Ralph Fingerhut, Matthias R. Baumgartner, Stefan Kölker, Georg F. Hoffmann, Gwendolyn Gramer, Jürgen G. OkunAbstract:Peak 1: 3-Hydroxypropionic Acid (3OHPA); peak 2: lactic Acid; peak 3: succinic Acid; peak 4: methylmalonic Acid (MMA); peaks 5a and 5b: methylcitric Acid (MCA); peak 0: interfering peak; DBS: dried blood spot; PA: propionic Acidemia. Numbered black peaks: unlabelled analytes; numbered unfilled peaks: deuterated internal standards. All intensities (Y-axes) have been normalized to 4*105 cps for better comparability (not on the inserted zoom-in figures) and have been plotted against retention time (X-axes). Chromatograms were generated by MassLynx 4.1 (Waters, Milford, MA, USA) and modified using Inkscape 0.91pre4 (Open Source Software licensed under the GNU General Public License).
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Biochemical pathway of propionate metabolism (simplified).
2017Co-Authors: Péter Monostori, Glynis Klinke, Sylvia Richter, Ákos Baráth, Ralph Fingerhut, Matthias R. Baumgartner, Stefan Kölker, Georg F. Hoffmann, Gwendolyn Gramer, Jürgen G. OkunAbstract:3OHPA: 3-Hydroxypropionic Acid; MMA: methylmalonic Acid; MCA: methylcitric Acid; Cbl: cobalamin. Drawn using Inkscape 0.91pre4 (Open Source Software licensed under the GNU General Public License).
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LC-MS/MS chromatogram of DBS from a healthy newborn.
2017Co-Authors: Péter Monostori, Glynis Klinke, Sylvia Richter, Ákos Baráth, Ralph Fingerhut, Matthias R. Baumgartner, Stefan Kölker, Georg F. Hoffmann, Gwendolyn Gramer, Jürgen G. OkunAbstract:Peak 1: 3-Hydroxypropionic Acid (3OHPA); peak 2: lactic Acid; peak 3: succinic Acid; peak 4: methylmalonic Acid (MMA); peaks 5a and 5b: methylcitric Acid (MCA); peak 0: interfering peak; DBS: dried blood spot. Numbered black peaks: unlabelled analytes; numbered unfilled peaks: deuterated internal standards. All intensities (Y-axes) have been normalized to 4*105 cps for better comparability (not on the inserted zoom-in figures) and have been plotted against retention time (X-axes). Chromatograms were generated by MassLynx 4.1 (Waters, Milford, MA, USA) and modified using Inkscape 0.91pre4 (Open Source Software licensed under the GNU General Public License).
Song Yang - One of the best experts on this subject based on the ideXlab platform.
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production of 3 hydroxypropionic Acid in engineered methylobacterium extorquens am1 and its reassimilation through a reductive route
Microbial Cell Factories, 2017Co-Authors: Yiming Yang, Wenjing Chen, Jing Yang, Yuanming Zhou, Min Zhang, Liping Zhu, Guangyuan Wang, Song YangAbstract:3-Hydroxypropionic Acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic Acid and 1,3-propanediol. Although Escherichia coli or Saccharomyces cerevisiae are the primary industrial microbes for the production of 3-HP, alternative engineered hosts have the potential to generate 3-HP from other carbon feedstocks. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating 3-HP from one-carbon feedstock methanol. Here we constructed a malonyl-CoA pathway by heterologously overexpressing the mcr gene to convert methanol into 3-HP in M. extorquens AM1. The engineered strains demonstrated 3-HP production with initial titer of 6.8 mg/l in shake flask cultivation, which was further improved to 69.8 mg/l by increasing the strength of promoter and mcr gene copy number. In vivo metabolic analysis showed a significant decrease of the acetyl-CoA pool size in the strain with the highest 3-HP titer, suggesting the supply of acetyl-CoA is a potential bottleneck for further improvement. Notably, 3-HP was rapidly degraded after the transition from exponential phase to stationary phase. Metabolomics analysis showed the accumulation of intracellular 3-hydroxypropionyl-CoA at stationary phase with the addition of 3-HP into the cultured medium, indicating 3-HP was first converted to its CoA derivatives. In vitro enzymatic assay and β-alanine pathway dependent 13C-labeling further demonstrated that a reductive route sequentially converted 3-HP-CoA to acrylyl-CoA and propionyl-CoA, with the latter being reassimilated into the ethylmalonyl-CoA pathway. The deletion of the gene META1_4251 encoding a putative acrylyl-CoA reductase led to reduced degradation rate of 3-HP in late stationary phase. We demonstrated the feasibility of constructing the malonyl-CoA pathway in M. extorquens AM1 to generate 3-HP. Furthermore, we showed that a reductive route coupled with the ethylmalonyl-CoA pathway was the major channel responsible for degradation of the 3-HP during the growth transition. Engineered M. extorquens AM1 represents a good platform for 3-HP production from methanol.
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Production of 3-Hydroxypropionic Acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route
BMC, 2017Co-Authors: Yiming Yang, Wenjing Chen, Jing Yang, Yuanming Zhou, Min Zhang, Liping Zhu, Guangyuan Wang, Song YangAbstract:Abstract Background 3-Hydroxypropionic Acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic Acid and 1,3-propanediol. Although Escherichia coli or Saccharomyces cerevisiae are the primary industrial microbes for the production of 3-HP, alternative engineered hosts have the potential to generate 3-HP from other carbon feedstocks. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating 3-HP from one-carbon feedstock methanol. Results Here we constructed a malonyl-CoA pathway by heterologously overexpressing the mcr gene to convert methanol into 3-HP in M. extorquens AM1. The engineered strains demonstrated 3-HP production with initial titer of 6.8 mg/l in shake flask cultivation, which was further improved to 69.8 mg/l by increasing the strength of promoter and mcr gene copy number. In vivo metabolic analysis showed a significant decrease of the acetyl-CoA pool size in the strain with the highest 3-HP titer, suggesting the supply of acetyl-CoA is a potential bottleneck for further improvement. Notably, 3-HP was rapidly degraded after the transition from exponential phase to stationary phase. Metabolomics analysis showed the accumulation of intracellular 3-hydroxypropionyl-CoA at stationary phase with the addition of 3-HP into the cultured medium, indicating 3-HP was first converted to its CoA derivatives. In vitro enzymatic assay and β-alanine pathway dependent 13C-labeling further demonstrated that a reductive route sequentially converted 3-HP-CoA to acrylyl-CoA and propionyl-CoA, with the latter being reassimilated into the ethylmalonyl-CoA pathway. The deletion of the gene META1_4251 encoding a putative acrylyl-CoA reductase led to reduced degradation rate of 3-HP in late stationary phase. Conclusions We demonstrated the feasibility of constructing the malonyl-CoA pathway in M. extorquens AM1 to generate 3-HP. Furthermore, we showed that a reductive route coupled with the ethylmalonyl-CoA pathway was the major channel responsible for degradation of the 3-HP during the growth transition. Engineered M. extorquens AM1 represents a good platform for 3-HP production from methanol
Gregoire Burge - One of the best experts on this subject based on the ideXlab platform.
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reactive extraction of bio based 3 hydroxypropionic Acid assisted by hollow fiber membrane contactor using toa and aliquat 336 in n decanol
Journal of Chemical Technology & Biotechnology, 2016Co-Authors: Gregoire Burge, Florian Chemarin, Marwen Moussa, Claire Saulouberion, Florent AllaisAbstract:BACKGROUND Reactive liquid–liquid extraction is a promising technique for the direct recovery of carboxylic Acids from bioconversion media. This work focused on the optimization of 3-Hydroxypropionic Acid (3-HP) reactive extraction assisted by hollow-fiber membrane contactor (HFMC), using tri-n-octylamine (TOA) and Aliquat 336 as extractants in n-decanol, and on its practical application for the recovery of 3-HP obtained from glycerol bioconversion by Lactobacillus reuteri. RESULTS The results underlined the influence of the initial pH and 3-HP concentration of the aqueous medium and the impact of extractant phase composition on the extraction performances. The highest distribution coefficient of 3-HP (KD = 13.0) was observed at pH = 3 with 10% v/v TOA mixed with 10% v/v Aliquat 336 in n-decanol. This study showed that the Acid concentration and the ratio of amines used between TOA and Aliquat 336 in n-decanol did not have a great impact on the KD during 3-HP reactive extraction, whereas it had an influence on the extraction kinetics. Moreover, 3-HP reactive extraction assisted by HFMC from real bioconversion broth with glycerol as the substrate was shown to be feasible, with the same order of magnitude of extraction kinetics but with a lower KD compared with reactive extraction using model broth. CONCLUSIONS 3-HP was predominantly extracted from the bioconversion broth, confirming the high selectivity of the extraction process studied and the high potential of this approach applied to the biotechnological production of 3-HP. © 2016 Society of Chemical Industry
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Reactive extraction of bio-based 3-Hydroxypropionic Acid assisted by hollow-fiber membrane contactor using TOA and Aliquat 336 in n-decanol
Journal of Chemical Technology and Biotechnology, 2016Co-Authors: Gregoire Burge, Florian Chemarin, Marwen Moussa, Florent Allais, Henry-eric Spinnler, Claire Saulou-bérion, Violaine Athes-dutourAbstract:BACKGROUND: Reactive liquid-liquid extraction is a promising technique for the direct recovery of carboxylic Acids from bioconversion media. This work focused on the optimization of 3-Hydroxypropionic Acid (3-HP) reactive extraction assisted by hollow-fiber membrane contactor (HFMC), using tri-n-octylamine (TOA) and Aliquat 336 as extractants in n-decanol, and on its practical application for the recovery of 3-HP obtained from glycerol bioconversion by Lactobacillus reuteri. RESULTS: The results underlined the influence of the initial pH and 3-HP concentration of the aqueous medium and the impact of extractant phase composition on the extraction performances. The highest distribution coefficient of 3-HP (K-D = 13.0) was observed at pH = 3 with 10% v/v TOA mixed with 10% v/v Aliquat 336 in n-decanol. This study showed that the Acid concentration and the ratio of amines used between TOA and Aliquat 336 in n-decanol did not have a great impact on the K-D during 3-HP reactive extraction, whereas it had an influence on the extraction kinetics. Moreover, 3-HP reactive extraction assisted by HFMC from real bioconversion broth with glycerol as the substrate was shown to be feasible, with the same order of magnitude of extraction kinetics but with a lower K-D compared with reactive extraction using model broth. CONCLUSIONS: 3-HP was predominantly extracted from the bioconversion broth, confirming the high selectivity of the extraction process studied and the high potential of this approach applied to the biotechnological production of 3-HP.
