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Xingdong Zhang - One of the best experts on this subject based on the ideXlab platform.
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effect of Phase Composition on protein adsorption and osteoinduction of porous calcium phosphate ceramics in mice
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Jing Wang, Ying Chen, Tun Yuan, Xingdong ZhangAbstract:The purpose of this study was to investigate the effect of Phase Compositions of porous calcium phosphate (CaP) ceramics on their protein adsorption behaviors in vitro and osteoinductive potentials in vivo in mice. Under competitive conditions, a high adsorption of bone morphogenetic protein 2 (BMP-2) was observed at a high initial concentration of BMP-2 in the multi-protein solution on all the four types of ceramics, indicating their strong affinity for BMP-2. No significant difference in BMP-2 adsorption between the ceramics was noted, indicating that Phase Composition could have little influence on BMP-2 adsorption. After implantation into the thigh muscles of mice for 45 and 90 days, the histological and histomorphometric analyses showed that porous biphasic calcium phosphate (BCP) ceramic consisting of 30% hydroxyapatite HA and 70% tricalcium phosphate (β-TCP), i.e. BCP-2 had stronger osteoinductive ability than the other three groups of ceramics. The immunohistochemical staining showed the highest expression of BMP-2 and osteocalcin (OCN) in BCP-2 group. Osteoinduction of porous CaP ceramics might be influenced by the amount of BMP-2 present in the local microenvironment in the implant, which was regulated by the Phase Composition of the ceramics. BCP-2 promoted the highest expression of BMP-2 and then showed the strongest osteoinduction in mice. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4234–4243, 2014.
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effect of Phase Composition on protein adsorption and osteoinduction of porous calcium phosphate ceramics in mice
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Jing Wang, Xiangdong Zhu, Yujiang Fan, Ying Chen, Tun Yuan, Yanfei Tan, Xingdong ZhangAbstract:The purpose of this study was to investigate the effect of Phase Compositions of porous calcium phosphate (CaP) ceramics on their protein adsorption behaviors in vitro and osteoinductive potentials in vivo in mice. Under competitive conditions, a high adsorption of bone morphogenetic protein 2 (BMP-2) was observed at a high initial concentration of BMP-2 in the multi-protein solution on all the four types of ceramics, indicating their strong affinity for BMP-2. No significant difference in BMP-2 adsorption between the ceramics was noted, indicating that Phase Composition could have little influence on BMP-2 adsorption. After implantation into the thigh muscles of mice for 45 and 90 days, the histological and histomorphometric analyses showed that porous biphasic calcium phosphate (BCP) ceramic consisting of 30% hydroxyapatite HA and 70% tricalcium phosphate (β-TCP), i.e. BCP-2 had stronger osteoinductive ability than the other three groups of ceramics. The immunohistochemical staining showed the highest expression of BMP-2 and osteocalcin (OCN) in BCP-2 group. Osteoinduction of porous CaP ceramics might be influenced by the amount of BMP-2 present in the local microenvironment in the implant, which was regulated by the Phase Composition of the ceramics. BCP-2 promoted the highest expression of BMP-2 and then showed the strongest osteoinduction in mice.
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dynamic competitive adsorption of bone related proteins on calcium phosphate ceramic particles with different Phase Composition and microstructure
Journal of Biomedical Materials Research Part B, 2013Co-Authors: Jing Wang, Xiangdong Zhu, Huijie Zhang, Hongsong Fan, Yujiang Fan, Xingdong ZhangAbstract:The biocompatibility and bioactivity of biomaterials used for hard tissue repair are closely related to their adsorption capacities for bone-related proteins. In the present study, three types of calcium phosphate (CaP) ceramic particles with different Phase Composition or microstructure were fabricated, and their protein adsorption abilities were investigated by a self-made device under the simulated dynamic physiological circumstance. The results of X-ray diffraction, field emission scanning electron microscopy, mercury penetration test, and nitrogen sorption test showed that the irregular hydroxyapatite (HA) ceramic particles obtained by conventional drying and sintering (named as HA-C) had fewer micropores and lower specific surface area (SSA) than did the spherical HA or biphasic calcium phosphate (BCP) ceramic particles made by spray drying and sintering (named as HA-S and BCP-S, respectively). The dynamic protein adsorption study proved that both the Phase Composition and microstructure of CaP ceramic particles affected their adsorption capacities for those bone-related proteins. The spherical HA-S and BCP-S particles with abundant micropores and high SSA showed higher adsorption of serum proteins, including fibronectin and vitronectin, than the irregular HA-C did. On the other hand, in spite of the relatively high concentration of bovine serum albumin (BSA) in the binary bone morphogenetic protein 2 (BMP-2)/BSA solution, BMP-2 adsorption on the three CaP ceramic particles increased with the increase in its initial concentration. Similarly, HA-S and BCP-S particles had a larger amount of the adsorbed BMP-2 per gram solid than HA-C did. Therefore, it could be believed that the difference of various CaP ceramics in the Phase Composition and microporous structure would affect their binding capacity for those bone-related proteins and thus lead to their difference in osteoinduction. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
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effect of Phase Composition and microstructure of calcium phosphate ceramic particles on protein adsorption
Acta Biomaterialia, 2010Co-Authors: Xiangdong Zhu, Huijie Zhang, H S Fan, Xingdong ZhangAbstract:Abstract The biological performance of biomaterials is strongly influenced by their protein adsorption characteristics, which are related to the structures and properties of both the biomaterial and the protein. In the present study two groups of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic powders were fabricated by different drying processes. The roles of the Phase Composition and microstructure of the powders in the adsorption of various model proteins were evaluated. The experimental results showed that BCP always had a higher ability to adsorb fibrinogen, insulin or type I collagen (Col-I) than HA. The microporosity and micropore size of the CaP particles also had a strong impact on their protein adsorption characteristics. HA and BCP particles with higher microporosities and/or more micropores >20 nm in diameter could adsorb more fibrinogen or insulin. However, amounts of adsorbed Col-I were largely unaffected by the microstructure of HA and BCP particles.
Jing Wang - One of the best experts on this subject based on the ideXlab platform.
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effect of Phase Composition on protein adsorption and osteoinduction of porous calcium phosphate ceramics in mice
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Jing Wang, Ying Chen, Tun Yuan, Xingdong ZhangAbstract:The purpose of this study was to investigate the effect of Phase Compositions of porous calcium phosphate (CaP) ceramics on their protein adsorption behaviors in vitro and osteoinductive potentials in vivo in mice. Under competitive conditions, a high adsorption of bone morphogenetic protein 2 (BMP-2) was observed at a high initial concentration of BMP-2 in the multi-protein solution on all the four types of ceramics, indicating their strong affinity for BMP-2. No significant difference in BMP-2 adsorption between the ceramics was noted, indicating that Phase Composition could have little influence on BMP-2 adsorption. After implantation into the thigh muscles of mice for 45 and 90 days, the histological and histomorphometric analyses showed that porous biphasic calcium phosphate (BCP) ceramic consisting of 30% hydroxyapatite HA and 70% tricalcium phosphate (β-TCP), i.e. BCP-2 had stronger osteoinductive ability than the other three groups of ceramics. The immunohistochemical staining showed the highest expression of BMP-2 and osteocalcin (OCN) in BCP-2 group. Osteoinduction of porous CaP ceramics might be influenced by the amount of BMP-2 present in the local microenvironment in the implant, which was regulated by the Phase Composition of the ceramics. BCP-2 promoted the highest expression of BMP-2 and then showed the strongest osteoinduction in mice. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4234–4243, 2014.
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effect of Phase Composition on protein adsorption and osteoinduction of porous calcium phosphate ceramics in mice
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Jing Wang, Xiangdong Zhu, Yujiang Fan, Ying Chen, Tun Yuan, Yanfei Tan, Xingdong ZhangAbstract:The purpose of this study was to investigate the effect of Phase Compositions of porous calcium phosphate (CaP) ceramics on their protein adsorption behaviors in vitro and osteoinductive potentials in vivo in mice. Under competitive conditions, a high adsorption of bone morphogenetic protein 2 (BMP-2) was observed at a high initial concentration of BMP-2 in the multi-protein solution on all the four types of ceramics, indicating their strong affinity for BMP-2. No significant difference in BMP-2 adsorption between the ceramics was noted, indicating that Phase Composition could have little influence on BMP-2 adsorption. After implantation into the thigh muscles of mice for 45 and 90 days, the histological and histomorphometric analyses showed that porous biphasic calcium phosphate (BCP) ceramic consisting of 30% hydroxyapatite HA and 70% tricalcium phosphate (β-TCP), i.e. BCP-2 had stronger osteoinductive ability than the other three groups of ceramics. The immunohistochemical staining showed the highest expression of BMP-2 and osteocalcin (OCN) in BCP-2 group. Osteoinduction of porous CaP ceramics might be influenced by the amount of BMP-2 present in the local microenvironment in the implant, which was regulated by the Phase Composition of the ceramics. BCP-2 promoted the highest expression of BMP-2 and then showed the strongest osteoinduction in mice.
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dynamic competitive adsorption of bone related proteins on calcium phosphate ceramic particles with different Phase Composition and microstructure
Journal of Biomedical Materials Research Part B, 2013Co-Authors: Jing Wang, Xiangdong Zhu, Huijie Zhang, Hongsong Fan, Yujiang Fan, Xingdong ZhangAbstract:The biocompatibility and bioactivity of biomaterials used for hard tissue repair are closely related to their adsorption capacities for bone-related proteins. In the present study, three types of calcium phosphate (CaP) ceramic particles with different Phase Composition or microstructure were fabricated, and their protein adsorption abilities were investigated by a self-made device under the simulated dynamic physiological circumstance. The results of X-ray diffraction, field emission scanning electron microscopy, mercury penetration test, and nitrogen sorption test showed that the irregular hydroxyapatite (HA) ceramic particles obtained by conventional drying and sintering (named as HA-C) had fewer micropores and lower specific surface area (SSA) than did the spherical HA or biphasic calcium phosphate (BCP) ceramic particles made by spray drying and sintering (named as HA-S and BCP-S, respectively). The dynamic protein adsorption study proved that both the Phase Composition and microstructure of CaP ceramic particles affected their adsorption capacities for those bone-related proteins. The spherical HA-S and BCP-S particles with abundant micropores and high SSA showed higher adsorption of serum proteins, including fibronectin and vitronectin, than the irregular HA-C did. On the other hand, in spite of the relatively high concentration of bovine serum albumin (BSA) in the binary bone morphogenetic protein 2 (BMP-2)/BSA solution, BMP-2 adsorption on the three CaP ceramic particles increased with the increase in its initial concentration. Similarly, HA-S and BCP-S particles had a larger amount of the adsorbed BMP-2 per gram solid than HA-C did. Therefore, it could be believed that the difference of various CaP ceramics in the Phase Composition and microporous structure would affect their binding capacity for those bone-related proteins and thus lead to their difference in osteoinduction. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
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Phase Composition and microwave dielectric properties of znal2o4 co2tio4 low permittivity ceramics with high quality factor
Journal of the American Ceramic Society, 2011Co-Authors: Wen Lei, Xiaohong Wang, Fei Liang, Jing WangAbstract:Phase Composition and microwave dielectric properties of (1−x)ZnAl2O4–xCo2TiO4 ceramics synthesized by a conventional solid-state reaction were investigated. ZnAl2O4 can form a solid solution with Co2TiO4 for x≤0.3 and x≥0.7, while subgrains with ZnAl2O4-based and Co2TiO4-based spinel Phase Composition appear alternately on the same large grains for 0.4≤x≤0.6. The ɛr and τf value of the (1−x)ZnAl2O4–xCo2TiO4 ceramics increase within the range of 8.3–16.9 and −71.3 to −40.8 ppm/°C with the increasing of x value, respectively. And the Q×f values with 147 600 GHz at x=0.2, 127 000 GHz at x=0.5, and 148 810 GHz at x=0.8 are all obviously higher than that of the end member ZnAl2O4 (96 800 GHz) and Co2TiO4 (98 060 GHz), which results from structural stability and lower inner stress.
Xiangdong Zhu - One of the best experts on this subject based on the ideXlab platform.
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effect of Phase Composition on protein adsorption and osteoinduction of porous calcium phosphate ceramics in mice
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Jing Wang, Xiangdong Zhu, Yujiang Fan, Ying Chen, Tun Yuan, Yanfei Tan, Xingdong ZhangAbstract:The purpose of this study was to investigate the effect of Phase Compositions of porous calcium phosphate (CaP) ceramics on their protein adsorption behaviors in vitro and osteoinductive potentials in vivo in mice. Under competitive conditions, a high adsorption of bone morphogenetic protein 2 (BMP-2) was observed at a high initial concentration of BMP-2 in the multi-protein solution on all the four types of ceramics, indicating their strong affinity for BMP-2. No significant difference in BMP-2 adsorption between the ceramics was noted, indicating that Phase Composition could have little influence on BMP-2 adsorption. After implantation into the thigh muscles of mice for 45 and 90 days, the histological and histomorphometric analyses showed that porous biphasic calcium phosphate (BCP) ceramic consisting of 30% hydroxyapatite HA and 70% tricalcium phosphate (β-TCP), i.e. BCP-2 had stronger osteoinductive ability than the other three groups of ceramics. The immunohistochemical staining showed the highest expression of BMP-2 and osteocalcin (OCN) in BCP-2 group. Osteoinduction of porous CaP ceramics might be influenced by the amount of BMP-2 present in the local microenvironment in the implant, which was regulated by the Phase Composition of the ceramics. BCP-2 promoted the highest expression of BMP-2 and then showed the strongest osteoinduction in mice.
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dynamic competitive adsorption of bone related proteins on calcium phosphate ceramic particles with different Phase Composition and microstructure
Journal of Biomedical Materials Research Part B, 2013Co-Authors: Jing Wang, Xiangdong Zhu, Huijie Zhang, Hongsong Fan, Yujiang Fan, Xingdong ZhangAbstract:The biocompatibility and bioactivity of biomaterials used for hard tissue repair are closely related to their adsorption capacities for bone-related proteins. In the present study, three types of calcium phosphate (CaP) ceramic particles with different Phase Composition or microstructure were fabricated, and their protein adsorption abilities were investigated by a self-made device under the simulated dynamic physiological circumstance. The results of X-ray diffraction, field emission scanning electron microscopy, mercury penetration test, and nitrogen sorption test showed that the irregular hydroxyapatite (HA) ceramic particles obtained by conventional drying and sintering (named as HA-C) had fewer micropores and lower specific surface area (SSA) than did the spherical HA or biphasic calcium phosphate (BCP) ceramic particles made by spray drying and sintering (named as HA-S and BCP-S, respectively). The dynamic protein adsorption study proved that both the Phase Composition and microstructure of CaP ceramic particles affected their adsorption capacities for those bone-related proteins. The spherical HA-S and BCP-S particles with abundant micropores and high SSA showed higher adsorption of serum proteins, including fibronectin and vitronectin, than the irregular HA-C did. On the other hand, in spite of the relatively high concentration of bovine serum albumin (BSA) in the binary bone morphogenetic protein 2 (BMP-2)/BSA solution, BMP-2 adsorption on the three CaP ceramic particles increased with the increase in its initial concentration. Similarly, HA-S and BCP-S particles had a larger amount of the adsorbed BMP-2 per gram solid than HA-C did. Therefore, it could be believed that the difference of various CaP ceramics in the Phase Composition and microporous structure would affect their binding capacity for those bone-related proteins and thus lead to their difference in osteoinduction. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
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effect of Phase Composition and microstructure of calcium phosphate ceramic particles on protein adsorption
Acta Biomaterialia, 2010Co-Authors: Xiangdong Zhu, Huijie Zhang, H S Fan, Xingdong ZhangAbstract:Abstract The biological performance of biomaterials is strongly influenced by their protein adsorption characteristics, which are related to the structures and properties of both the biomaterial and the protein. In the present study two groups of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic powders were fabricated by different drying processes. The roles of the Phase Composition and microstructure of the powders in the adsorption of various model proteins were evaluated. The experimental results showed that BCP always had a higher ability to adsorb fibrinogen, insulin or type I collagen (Col-I) than HA. The microporosity and micropore size of the CaP particles also had a strong impact on their protein adsorption characteristics. HA and BCP particles with higher microporosities and/or more micropores >20 nm in diameter could adsorb more fibrinogen or insulin. However, amounts of adsorbed Col-I were largely unaffected by the microstructure of HA and BCP particles.
Yujiang Fan - One of the best experts on this subject based on the ideXlab platform.
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effect of Phase Composition on protein adsorption and osteoinduction of porous calcium phosphate ceramics in mice
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Jing Wang, Xiangdong Zhu, Yujiang Fan, Ying Chen, Tun Yuan, Yanfei Tan, Xingdong ZhangAbstract:The purpose of this study was to investigate the effect of Phase Compositions of porous calcium phosphate (CaP) ceramics on their protein adsorption behaviors in vitro and osteoinductive potentials in vivo in mice. Under competitive conditions, a high adsorption of bone morphogenetic protein 2 (BMP-2) was observed at a high initial concentration of BMP-2 in the multi-protein solution on all the four types of ceramics, indicating their strong affinity for BMP-2. No significant difference in BMP-2 adsorption between the ceramics was noted, indicating that Phase Composition could have little influence on BMP-2 adsorption. After implantation into the thigh muscles of mice for 45 and 90 days, the histological and histomorphometric analyses showed that porous biphasic calcium phosphate (BCP) ceramic consisting of 30% hydroxyapatite HA and 70% tricalcium phosphate (β-TCP), i.e. BCP-2 had stronger osteoinductive ability than the other three groups of ceramics. The immunohistochemical staining showed the highest expression of BMP-2 and osteocalcin (OCN) in BCP-2 group. Osteoinduction of porous CaP ceramics might be influenced by the amount of BMP-2 present in the local microenvironment in the implant, which was regulated by the Phase Composition of the ceramics. BCP-2 promoted the highest expression of BMP-2 and then showed the strongest osteoinduction in mice.
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dynamic competitive adsorption of bone related proteins on calcium phosphate ceramic particles with different Phase Composition and microstructure
Journal of Biomedical Materials Research Part B, 2013Co-Authors: Jing Wang, Xiangdong Zhu, Huijie Zhang, Hongsong Fan, Yujiang Fan, Xingdong ZhangAbstract:The biocompatibility and bioactivity of biomaterials used for hard tissue repair are closely related to their adsorption capacities for bone-related proteins. In the present study, three types of calcium phosphate (CaP) ceramic particles with different Phase Composition or microstructure were fabricated, and their protein adsorption abilities were investigated by a self-made device under the simulated dynamic physiological circumstance. The results of X-ray diffraction, field emission scanning electron microscopy, mercury penetration test, and nitrogen sorption test showed that the irregular hydroxyapatite (HA) ceramic particles obtained by conventional drying and sintering (named as HA-C) had fewer micropores and lower specific surface area (SSA) than did the spherical HA or biphasic calcium phosphate (BCP) ceramic particles made by spray drying and sintering (named as HA-S and BCP-S, respectively). The dynamic protein adsorption study proved that both the Phase Composition and microstructure of CaP ceramic particles affected their adsorption capacities for those bone-related proteins. The spherical HA-S and BCP-S particles with abundant micropores and high SSA showed higher adsorption of serum proteins, including fibronectin and vitronectin, than the irregular HA-C did. On the other hand, in spite of the relatively high concentration of bovine serum albumin (BSA) in the binary bone morphogenetic protein 2 (BMP-2)/BSA solution, BMP-2 adsorption on the three CaP ceramic particles increased with the increase in its initial concentration. Similarly, HA-S and BCP-S particles had a larger amount of the adsorbed BMP-2 per gram solid than HA-C did. Therefore, it could be believed that the difference of various CaP ceramics in the Phase Composition and microporous structure would affect their binding capacity for those bone-related proteins and thus lead to their difference in osteoinduction. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Huijie Zhang - One of the best experts on this subject based on the ideXlab platform.
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dynamic competitive adsorption of bone related proteins on calcium phosphate ceramic particles with different Phase Composition and microstructure
Journal of Biomedical Materials Research Part B, 2013Co-Authors: Jing Wang, Xiangdong Zhu, Huijie Zhang, Hongsong Fan, Yujiang Fan, Xingdong ZhangAbstract:The biocompatibility and bioactivity of biomaterials used for hard tissue repair are closely related to their adsorption capacities for bone-related proteins. In the present study, three types of calcium phosphate (CaP) ceramic particles with different Phase Composition or microstructure were fabricated, and their protein adsorption abilities were investigated by a self-made device under the simulated dynamic physiological circumstance. The results of X-ray diffraction, field emission scanning electron microscopy, mercury penetration test, and nitrogen sorption test showed that the irregular hydroxyapatite (HA) ceramic particles obtained by conventional drying and sintering (named as HA-C) had fewer micropores and lower specific surface area (SSA) than did the spherical HA or biphasic calcium phosphate (BCP) ceramic particles made by spray drying and sintering (named as HA-S and BCP-S, respectively). The dynamic protein adsorption study proved that both the Phase Composition and microstructure of CaP ceramic particles affected their adsorption capacities for those bone-related proteins. The spherical HA-S and BCP-S particles with abundant micropores and high SSA showed higher adsorption of serum proteins, including fibronectin and vitronectin, than the irregular HA-C did. On the other hand, in spite of the relatively high concentration of bovine serum albumin (BSA) in the binary bone morphogenetic protein 2 (BMP-2)/BSA solution, BMP-2 adsorption on the three CaP ceramic particles increased with the increase in its initial concentration. Similarly, HA-S and BCP-S particles had a larger amount of the adsorbed BMP-2 per gram solid than HA-C did. Therefore, it could be believed that the difference of various CaP ceramics in the Phase Composition and microporous structure would affect their binding capacity for those bone-related proteins and thus lead to their difference in osteoinduction. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
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effect of Phase Composition and microstructure of calcium phosphate ceramic particles on protein adsorption
Acta Biomaterialia, 2010Co-Authors: Xiangdong Zhu, Huijie Zhang, H S Fan, Xingdong ZhangAbstract:Abstract The biological performance of biomaterials is strongly influenced by their protein adsorption characteristics, which are related to the structures and properties of both the biomaterial and the protein. In the present study two groups of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic powders were fabricated by different drying processes. The roles of the Phase Composition and microstructure of the powders in the adsorption of various model proteins were evaluated. The experimental results showed that BCP always had a higher ability to adsorb fibrinogen, insulin or type I collagen (Col-I) than HA. The microporosity and micropore size of the CaP particles also had a strong impact on their protein adsorption characteristics. HA and BCP particles with higher microporosities and/or more micropores >20 nm in diameter could adsorb more fibrinogen or insulin. However, amounts of adsorbed Col-I were largely unaffected by the microstructure of HA and BCP particles.
