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7 Nitroindazole

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Yuri Zagvazdin – 1st expert on this subject based on the ideXlab platform

  • on the selectivity of 7 Nitroindazole as an inhibitor of neuronal nitric oxide synthase
    Trends in Pharmacological Sciences, 1998
    Co-Authors: Anton Reiner, Yuri Zagvazdin

    Abstract:

    Abstract Not only neuronal nitric oxide synthase (NOS) but endothelial NOS is inhibited by 7Nitroindazole in vivo

  • evidence from its cardiovascular effects that 7 Nitroindazole may inhibit endothelial nitric oxide synthase in vivo
    European Journal of Pharmacology, 1996
    Co-Authors: Yuri Zagvazdin, Anton Reiner, Giuseppe Sancesario, Yixin Wang, Leonard Share, Malinda E C Fitzgerald

    Abstract:

    Abstract We have examined whether the cardiovascular effects of 7Nitroindazole, a reportedly selective inhibitor of neuronal nitric oxide (NO) synthase, are induced without inhibition of endothelial NO synthase. A significant increase in mean arterial blood pressure but no change in heart rate was observed after 7Nitroindazole administration (50 mg/kg i.p.) in rats anesthetized with urethane or urethane and chloralose, while both an elevation in mean arterial blood pressure and bradycardia were observed in conscious animals after 7Nitroindazole administration (50 mg/kg i.p.). No enhancements in these effects on mean arterial blood pressure and heart rate were observed in urethane-chloralose anesthetized rats treated with a higher dose of 7Nitroindazole (75 mg/kg i.p.). Use of halothane to induce anesthesia abolished the pressor effect of 7Nitroindazole in rats studied under urethane anesthesia. 7Nitroindazole shortened the duration of the acetylcholine (3 μg or 30 μg i.v.) but not the sodium nitroprusside (2 μg i.v.) induced hypotension in urethane-anesthetized rats. Pretreatment with l -arginine (300 mg/kg i.v.) inhibited the effects of 7Nitroindazole on mean arterial blood pressure and acetylcholine induced hypotension, suggesting involvement of the l -arginine-NO pathway in the effects of 7Nitroindazole. The effects of 7Nitroindazole on blood pressure and on the depressor responses to acetylcholine and sodium nitroprusside are similar to the effects previously observed after non-selective NO synthase inhibition by l -arginine analogs. Our results suggest, therefore, that 7Nitroindazole affects basal endothelial NO formation in vivo. The suppressive action of halothane on the cardiovascular effects of 7Nitroindazole suggests that the influence of anesthetics should be taken into consideration in studies of the cardiovascular effects of NO synthase inhibitors, particularly 7Nitroindazole.

Anton Reiner – 2nd expert on this subject based on the ideXlab platform

  • on the selectivity of 7 Nitroindazole as an inhibitor of neuronal nitric oxide synthase
    Trends in Pharmacological Sciences, 1998
    Co-Authors: Anton Reiner, Yuri Zagvazdin

    Abstract:

    Abstract Not only neuronal nitric oxide synthase (NOS) but endothelial NOS is inhibited by 7Nitroindazole in vivo

  • evidence from its cardiovascular effects that 7 Nitroindazole may inhibit endothelial nitric oxide synthase in vivo
    European Journal of Pharmacology, 1996
    Co-Authors: Yuri Zagvazdin, Anton Reiner, Giuseppe Sancesario, Yixin Wang, Leonard Share, Malinda E C Fitzgerald

    Abstract:

    Abstract We have examined whether the cardiovascular effects of 7Nitroindazole, a reportedly selective inhibitor of neuronal nitric oxide (NO) synthase, are induced without inhibition of endothelial NO synthase. A significant increase in mean arterial blood pressure but no change in heart rate was observed after 7Nitroindazole administration (50 mg/kg i.p.) in rats anesthetized with urethane or urethane and chloralose, while both an elevation in mean arterial blood pressure and bradycardia were observed in conscious animals after 7Nitroindazole administration (50 mg/kg i.p.). No enhancements in these effects on mean arterial blood pressure and heart rate were observed in urethane-chloralose anesthetized rats treated with a higher dose of 7Nitroindazole (75 mg/kg i.p.). Use of halothane to induce anesthesia abolished the pressor effect of 7Nitroindazole in rats studied under urethane anesthesia. 7Nitroindazole shortened the duration of the acetylcholine (3 μg or 30 μg i.v.) but not the sodium nitroprusside (2 μg i.v.) induced hypotension in urethane-anesthetized rats. Pretreatment with l -arginine (300 mg/kg i.v.) inhibited the effects of 7Nitroindazole on mean arterial blood pressure and acetylcholine induced hypotension, suggesting involvement of the l -arginine-NO pathway in the effects of 7Nitroindazole. The effects of 7Nitroindazole on blood pressure and on the depressor responses to acetylcholine and sodium nitroprusside are similar to the effects previously observed after non-selective NO synthase inhibition by l -arginine analogs. Our results suggest, therefore, that 7Nitroindazole affects basal endothelial NO formation in vivo. The suppressive action of halothane on the cardiovascular effects of 7Nitroindazole suggests that the influence of anesthetics should be taken into consideration in studies of the cardiovascular effects of NO synthase inhibitors, particularly 7Nitroindazole.

Paul A T Kelly – 3rd expert on this subject based on the ideXlab platform

  • 7 Nitroindazole reduces cerebral blood flow following chronic nitric oxide synthase inhibition
    Brain Research, 2000
    Co-Authors: Paul A T Kelly, Isobel M Ritchie, Douglas E Mcbean

    Abstract:

    Abstract Blood flow and glucose utilization were measured in rat brain after chronic l -NAME treatment followed by acute 7Nitroindazole. Following chronic l -NAME, blood flow was not significantly different from control. Treatment with acute 7Nitroindazole reduced blood flow to the same extent in both chronic saline and l -NAME groups. Glucose utilization was unaffected. These results suggest that residual NOS activity in brain is sufficient to provide tonic, NO-dependent cerebrovascular dilator tone.

  • inhibition of neuronal nitric oxide synthase by 7 Nitroindazole effects upon local cerebral blood flow and glucose use in the rat
    Journal of Cerebral Blood Flow and Metabolism, 1995
    Co-Authors: Paul A T Kelly, Isobel M Ritchie, G W Arbuthnott

    Abstract:

    The novel nitric oxide synthase inhibitor 7Nitroindazole (7-NI) is relatively specific for the neuronal isoform of the enzyme and in this study we have used this compound to investigate the physiological role of perivascular nitric oxide-containing nerves in the cerebrovascular bed. Following injection of 7-NI (25 or 50 mg/kg, i.p.), cerebral blood flow and glucose utilization were measured in the conscious rat using the fully quantitative [14C]iodoantipyrine and 2-[14C]deoxyglucose techniques, respectively. Neither dose of the drug produced any change in arterial blood pressure, confirming a lack of effect upon the endothelial isoform of the enzyme, although there was a pronounced decrease in heart rate (−28% by 10 min postinjection). Throughout the brain 25 mg/kg 7-NI i.p. resulted in decreases in blood flow of between −20% in the hippocampus and −58% in the substantia nigra. Increasing the dose to 50 mg/kg resulted in a further generalized decrease, to almost −60% in parts of the thalamus and hippocam…