The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Mingchun Liu - One of the best experts on this subject based on the ideXlab platform.
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the regulatory effect of flavonoids extracted from Abutilon Theophrasti leaves on gene expression in lps induced ali mice via the nf κb and mapk signaling pathways
Pharmaceutical Biology, 2019Co-Authors: Chunlian Tian, Cancan Cui, Xiaoyun Chen, Yu Chang, Ruxia Wang, Jing Ning, Mingchun LiuAbstract:Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon Theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regulatory effect of a TFE from Abutilon Theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways. Materials and methods: Kunming mice were intragastrically administered TFE (0.25, 0.5, 1.0 g/kg) for 5 days, and then ALI was induced via intranasal administration 40 μg of LPS in 10 μL PBS after intragastric administration on the 5th day, and PBS and DEX (2 mg/kg) were negative and positive control groups, respectively. Results: The relative expression of iNOS gene was 0.707, 0.507 and 0.483 for 0.25, 0.5 and 1.0 g/kg TFE, and COX-2 gene expression was also reduced after treatment by three concentrations of TFE with 0.768, 0.545, and 0.478. The mRNA expression levels of p65 were 0.61, 0.43 and 0.27 for 0.25, 0.5 and 1.0 g/kg TFE and IκB levels were increased in a dose-dependent manner with 3.99, 13.69 and 34.36. 0.5 and 1.0 g/kg TFE inhibited the expression of ERK1/2 with 0.59 and 0.38, p38MAPK with 0.62 and 0.54, and JNK with 0.37 and 0.29, and JNK mRNA expression was 0.60 for 0.25 g/kg TFE. Discussion and conclusion: These results indicate that the regulatory mechanisms of TFE on gene expression in LPS-induced ALI mice include inhibition of the NF-κB and MAPK signaling pathways.
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Abutilon Theophrasti medic episperms as a total flavonoids fraction for pharmaceutical applications in vitro antioxidant antibacterial anti inflammatory activities extraction technology and hplc ms profiles
Industrial Crops and Products, 2019Co-Authors: Chunlian Tian, Hong Wang, Yuru Guo, Peng Qiu, Cancan Cui, Mingchun LiuAbstract:Abstract Abutilon Theophrasti Medic. is widely distributed around the world, and the episperms part is rich in phenols and flavonoids compounds. The current study focused on the extraction technology, components analysis, in vitro antioxidant, antibacterial, and anti-inflammatory activities of total flavonoids from Abutilon Theophrasti Medic. episperms. The extraction process of total flavonoids fraction was optimized by response surface method, and the antioxidant, antibacterial, and anti-inflammatory activities were evaluated with the assay of radical scavenging activity and reducing power, micro-dilution broth method, and lipopolysaccharide induced mouse mononuclear macrophage leukemia cells RAW 264.7 model, respectively. The maximum extraction yield was achieved with concentration of ethanol solution 10%, ratio of solvent to material 30:1 ml/g, and ultrasonic extraction time 29.52 min. Moreover, seven compositions from total flavonoids fraction were detected separately, and four compounds were identified by high performance liquid chromatography-diode array detector-electrospray ionization-mass spectran. In addition, total flavonoids fraction from Abutilon Theophrasti Medic. episperms exhibited a better antioxidant activity demonstrated by ferric ion reducing antioxidant power, 2, 2′ - azino - bis (3-ethylbenzothiazoline -6-sulfonic acid diammonium salt, and 2, 2 -diphenyl -1-picrylhydrazyl radicals scavenging ability, and antibacterial against Escherichia coli, Salmonella, Staphyloccocus aureus, and Streptococcus, and anti-inflammatory activities in lipopolysaccharide induced RAW 264.7 cells. In a word, the present findings suggest that the total flavonoids fraction from Abutilon Theophrasti Medic. episperms was a very interesting candidate for the research and development of natural and healthy antioxidant, antibacterial, and anti-inflammatory agent for food, pharmaceutical, and medicine industries.
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the protective effect of the flavonoid fraction of Abutilon Theophrasti medic leaves on lps induced acute lung injury in mice via the nf κb and mapk signalling pathways
Biomedicine & Pharmacotherapy, 2019Co-Authors: Chunlian Tian, Peng Zhang, Hong Wang, Yuru Guo, Jie Yang, Zehui Zhang, Mingchun LiuAbstract:Abstract Accompanied by the damages of epithelial and capillary endothelial cell, acute lung injury is diagnosed with the typical pathological symptoms in clinic, including diffusing of pulmonary interstitial, alveolar oedema and hypoxic respiratory insufficiency. Current study focused on the investigation the anti-inflammatory action and mechanisms of total flavonoids extract (TFE) from Abutilon Theophrasti Medic. leaves on ALI mice induced by LPSs. Mice were administrated intragastrically with TFE at the concentrations of 0.25, 0.5, or 1.0 g/kg for 5 days, and on last day, nasal administration of LPSs for 6 h after 30 min for intragastric administration of TFE. Pretreatment with TFE not only reduced oxidative damage but also alleviated lung edema in ALI mice. Increased secretion of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, caused by LPSs was reversed by TFE; on the contrary, the anti-inflammatory cytokine IL-10 was upregulated. The proteins expressions of pro-inflammatory mediators iNOS and COX-2 induced by LPSs, were down-regulated by TFE. Moreover, the activation of NF-κB and MAPK signalling pathways was inhibited by TFE in LPSs induced ALI mice. The results revealed that the anti-inflammatory mechanisms of TFE were via inhibition of NF-κB and MAPK activation. Combined, the results suggested that TFE might exert in vivo antioxidant and anti-inflammatory functions in LPSs stimulated mice, and will be potential in adjuvant treatment in oxidative stress and inflammation diseases.
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Extraction Process, Component Analysis, and In Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon Theophrasti Medic. Leaves.
Mediators of inflammation, 2018Co-Authors: Chunlian Tian, Peng Zhang, Caixia Yang, Xiang Gao, Hong Wang, Yuru Guo, Mingchun LiuAbstract:The flavonoid fraction was extracted from the leaves of Abutilon Theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.
Chunlian Tian - One of the best experts on this subject based on the ideXlab platform.
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the regulatory effect of flavonoids extracted from Abutilon Theophrasti leaves on gene expression in lps induced ali mice via the nf κb and mapk signaling pathways
Pharmaceutical Biology, 2019Co-Authors: Chunlian Tian, Cancan Cui, Xiaoyun Chen, Yu Chang, Ruxia Wang, Jing Ning, Mingchun LiuAbstract:Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon Theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regulatory effect of a TFE from Abutilon Theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways. Materials and methods: Kunming mice were intragastrically administered TFE (0.25, 0.5, 1.0 g/kg) for 5 days, and then ALI was induced via intranasal administration 40 μg of LPS in 10 μL PBS after intragastric administration on the 5th day, and PBS and DEX (2 mg/kg) were negative and positive control groups, respectively. Results: The relative expression of iNOS gene was 0.707, 0.507 and 0.483 for 0.25, 0.5 and 1.0 g/kg TFE, and COX-2 gene expression was also reduced after treatment by three concentrations of TFE with 0.768, 0.545, and 0.478. The mRNA expression levels of p65 were 0.61, 0.43 and 0.27 for 0.25, 0.5 and 1.0 g/kg TFE and IκB levels were increased in a dose-dependent manner with 3.99, 13.69 and 34.36. 0.5 and 1.0 g/kg TFE inhibited the expression of ERK1/2 with 0.59 and 0.38, p38MAPK with 0.62 and 0.54, and JNK with 0.37 and 0.29, and JNK mRNA expression was 0.60 for 0.25 g/kg TFE. Discussion and conclusion: These results indicate that the regulatory mechanisms of TFE on gene expression in LPS-induced ALI mice include inhibition of the NF-κB and MAPK signaling pathways.
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Abutilon Theophrasti medic episperms as a total flavonoids fraction for pharmaceutical applications in vitro antioxidant antibacterial anti inflammatory activities extraction technology and hplc ms profiles
Industrial Crops and Products, 2019Co-Authors: Chunlian Tian, Hong Wang, Yuru Guo, Peng Qiu, Cancan Cui, Mingchun LiuAbstract:Abstract Abutilon Theophrasti Medic. is widely distributed around the world, and the episperms part is rich in phenols and flavonoids compounds. The current study focused on the extraction technology, components analysis, in vitro antioxidant, antibacterial, and anti-inflammatory activities of total flavonoids from Abutilon Theophrasti Medic. episperms. The extraction process of total flavonoids fraction was optimized by response surface method, and the antioxidant, antibacterial, and anti-inflammatory activities were evaluated with the assay of radical scavenging activity and reducing power, micro-dilution broth method, and lipopolysaccharide induced mouse mononuclear macrophage leukemia cells RAW 264.7 model, respectively. The maximum extraction yield was achieved with concentration of ethanol solution 10%, ratio of solvent to material 30:1 ml/g, and ultrasonic extraction time 29.52 min. Moreover, seven compositions from total flavonoids fraction were detected separately, and four compounds were identified by high performance liquid chromatography-diode array detector-electrospray ionization-mass spectran. In addition, total flavonoids fraction from Abutilon Theophrasti Medic. episperms exhibited a better antioxidant activity demonstrated by ferric ion reducing antioxidant power, 2, 2′ - azino - bis (3-ethylbenzothiazoline -6-sulfonic acid diammonium salt, and 2, 2 -diphenyl -1-picrylhydrazyl radicals scavenging ability, and antibacterial against Escherichia coli, Salmonella, Staphyloccocus aureus, and Streptococcus, and anti-inflammatory activities in lipopolysaccharide induced RAW 264.7 cells. In a word, the present findings suggest that the total flavonoids fraction from Abutilon Theophrasti Medic. episperms was a very interesting candidate for the research and development of natural and healthy antioxidant, antibacterial, and anti-inflammatory agent for food, pharmaceutical, and medicine industries.
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the protective effect of the flavonoid fraction of Abutilon Theophrasti medic leaves on lps induced acute lung injury in mice via the nf κb and mapk signalling pathways
Biomedicine & Pharmacotherapy, 2019Co-Authors: Chunlian Tian, Peng Zhang, Hong Wang, Yuru Guo, Jie Yang, Zehui Zhang, Mingchun LiuAbstract:Abstract Accompanied by the damages of epithelial and capillary endothelial cell, acute lung injury is diagnosed with the typical pathological symptoms in clinic, including diffusing of pulmonary interstitial, alveolar oedema and hypoxic respiratory insufficiency. Current study focused on the investigation the anti-inflammatory action and mechanisms of total flavonoids extract (TFE) from Abutilon Theophrasti Medic. leaves on ALI mice induced by LPSs. Mice were administrated intragastrically with TFE at the concentrations of 0.25, 0.5, or 1.0 g/kg for 5 days, and on last day, nasal administration of LPSs for 6 h after 30 min for intragastric administration of TFE. Pretreatment with TFE not only reduced oxidative damage but also alleviated lung edema in ALI mice. Increased secretion of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, caused by LPSs was reversed by TFE; on the contrary, the anti-inflammatory cytokine IL-10 was upregulated. The proteins expressions of pro-inflammatory mediators iNOS and COX-2 induced by LPSs, were down-regulated by TFE. Moreover, the activation of NF-κB and MAPK signalling pathways was inhibited by TFE in LPSs induced ALI mice. The results revealed that the anti-inflammatory mechanisms of TFE were via inhibition of NF-κB and MAPK activation. Combined, the results suggested that TFE might exert in vivo antioxidant and anti-inflammatory functions in LPSs stimulated mice, and will be potential in adjuvant treatment in oxidative stress and inflammation diseases.
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Extraction Process, Component Analysis, and In Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon Theophrasti Medic. Leaves.
Mediators of inflammation, 2018Co-Authors: Chunlian Tian, Peng Zhang, Caixia Yang, Xiang Gao, Hong Wang, Yuru Guo, Mingchun LiuAbstract:The flavonoid fraction was extracted from the leaves of Abutilon Theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.
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hplc quantification of nine chemical constituents from the five parts of Abutilon Theophrasti medic
Journal of Chromatographic Science, 2014Co-Authors: Chunlian Tian, Miao Wang, Xiaokun Liu, Haiping Wang, Chunjie ZhaoAbstract:A method of reversed-phase high-performance liquid chromatography (RP-HPLC) was established for the simultaneous determination of gallic acid, protocatechuic acid, catechin, vanillic acid, caffeic acid, ferulic acid, rutin, quercetin and syriacusin A in the ethanolic extracts from the five parts (roots, stems, leaves, seeds and exocarps) of Abutilon Theophrasti Medic. The nine components in the sample were extracted with 70% ethanol solution in an ultrasonic bath for 25 min and chromatographically separated on a C18 analytical column (250 3 4.6 mm i.d., 5 mm) by gradient elution with water containing 0.1% (v/v) formic acid (pH 2.4) and acetonitrile as mobile phases, at a flow rate of 1.0 mL/min. The detection was conducted by ultraviolet-visible absorption. The proposed method showed good linearity between the peak area of each analyte and its concentration. Relative standard deviations of the instrumental intra-day and inter-day precision and method precision were less than 2.0, 3.5 and 2.0%. Recoveries were within the range of 87.3‐100.8%. Limits of detection and quantification were 0.045‐0.400 mg/mL (signal-tonoise ratio: 3) and 0.090‐1.115 mg/mL (signal-to-noise ratio: 10), respectively. The proposed method was successfully applied to determine common phenols and flavonoids of Abutilon Theophrasti Medic. The results indicated that the RP-HPLC system may provide a rapid method for the quality control of Abutilon Theophrasti Medic.
Hong Wang - One of the best experts on this subject based on the ideXlab platform.
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Abutilon Theophrasti medic episperms as a total flavonoids fraction for pharmaceutical applications in vitro antioxidant antibacterial anti inflammatory activities extraction technology and hplc ms profiles
Industrial Crops and Products, 2019Co-Authors: Chunlian Tian, Hong Wang, Yuru Guo, Peng Qiu, Cancan Cui, Mingchun LiuAbstract:Abstract Abutilon Theophrasti Medic. is widely distributed around the world, and the episperms part is rich in phenols and flavonoids compounds. The current study focused on the extraction technology, components analysis, in vitro antioxidant, antibacterial, and anti-inflammatory activities of total flavonoids from Abutilon Theophrasti Medic. episperms. The extraction process of total flavonoids fraction was optimized by response surface method, and the antioxidant, antibacterial, and anti-inflammatory activities were evaluated with the assay of radical scavenging activity and reducing power, micro-dilution broth method, and lipopolysaccharide induced mouse mononuclear macrophage leukemia cells RAW 264.7 model, respectively. The maximum extraction yield was achieved with concentration of ethanol solution 10%, ratio of solvent to material 30:1 ml/g, and ultrasonic extraction time 29.52 min. Moreover, seven compositions from total flavonoids fraction were detected separately, and four compounds were identified by high performance liquid chromatography-diode array detector-electrospray ionization-mass spectran. In addition, total flavonoids fraction from Abutilon Theophrasti Medic. episperms exhibited a better antioxidant activity demonstrated by ferric ion reducing antioxidant power, 2, 2′ - azino - bis (3-ethylbenzothiazoline -6-sulfonic acid diammonium salt, and 2, 2 -diphenyl -1-picrylhydrazyl radicals scavenging ability, and antibacterial against Escherichia coli, Salmonella, Staphyloccocus aureus, and Streptococcus, and anti-inflammatory activities in lipopolysaccharide induced RAW 264.7 cells. In a word, the present findings suggest that the total flavonoids fraction from Abutilon Theophrasti Medic. episperms was a very interesting candidate for the research and development of natural and healthy antioxidant, antibacterial, and anti-inflammatory agent for food, pharmaceutical, and medicine industries.
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the protective effect of the flavonoid fraction of Abutilon Theophrasti medic leaves on lps induced acute lung injury in mice via the nf κb and mapk signalling pathways
Biomedicine & Pharmacotherapy, 2019Co-Authors: Chunlian Tian, Peng Zhang, Hong Wang, Yuru Guo, Jie Yang, Zehui Zhang, Mingchun LiuAbstract:Abstract Accompanied by the damages of epithelial and capillary endothelial cell, acute lung injury is diagnosed with the typical pathological symptoms in clinic, including diffusing of pulmonary interstitial, alveolar oedema and hypoxic respiratory insufficiency. Current study focused on the investigation the anti-inflammatory action and mechanisms of total flavonoids extract (TFE) from Abutilon Theophrasti Medic. leaves on ALI mice induced by LPSs. Mice were administrated intragastrically with TFE at the concentrations of 0.25, 0.5, or 1.0 g/kg for 5 days, and on last day, nasal administration of LPSs for 6 h after 30 min for intragastric administration of TFE. Pretreatment with TFE not only reduced oxidative damage but also alleviated lung edema in ALI mice. Increased secretion of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, caused by LPSs was reversed by TFE; on the contrary, the anti-inflammatory cytokine IL-10 was upregulated. The proteins expressions of pro-inflammatory mediators iNOS and COX-2 induced by LPSs, were down-regulated by TFE. Moreover, the activation of NF-κB and MAPK signalling pathways was inhibited by TFE in LPSs induced ALI mice. The results revealed that the anti-inflammatory mechanisms of TFE were via inhibition of NF-κB and MAPK activation. Combined, the results suggested that TFE might exert in vivo antioxidant and anti-inflammatory functions in LPSs stimulated mice, and will be potential in adjuvant treatment in oxidative stress and inflammation diseases.
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Extraction Process, Component Analysis, and In Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon Theophrasti Medic. Leaves.
Mediators of inflammation, 2018Co-Authors: Chunlian Tian, Peng Zhang, Caixia Yang, Xiang Gao, Hong Wang, Yuru Guo, Mingchun LiuAbstract:The flavonoid fraction was extracted from the leaves of Abutilon Theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.
Yuru Guo - One of the best experts on this subject based on the ideXlab platform.
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Abutilon Theophrasti medic episperms as a total flavonoids fraction for pharmaceutical applications in vitro antioxidant antibacterial anti inflammatory activities extraction technology and hplc ms profiles
Industrial Crops and Products, 2019Co-Authors: Chunlian Tian, Hong Wang, Yuru Guo, Peng Qiu, Cancan Cui, Mingchun LiuAbstract:Abstract Abutilon Theophrasti Medic. is widely distributed around the world, and the episperms part is rich in phenols and flavonoids compounds. The current study focused on the extraction technology, components analysis, in vitro antioxidant, antibacterial, and anti-inflammatory activities of total flavonoids from Abutilon Theophrasti Medic. episperms. The extraction process of total flavonoids fraction was optimized by response surface method, and the antioxidant, antibacterial, and anti-inflammatory activities were evaluated with the assay of radical scavenging activity and reducing power, micro-dilution broth method, and lipopolysaccharide induced mouse mononuclear macrophage leukemia cells RAW 264.7 model, respectively. The maximum extraction yield was achieved with concentration of ethanol solution 10%, ratio of solvent to material 30:1 ml/g, and ultrasonic extraction time 29.52 min. Moreover, seven compositions from total flavonoids fraction were detected separately, and four compounds were identified by high performance liquid chromatography-diode array detector-electrospray ionization-mass spectran. In addition, total flavonoids fraction from Abutilon Theophrasti Medic. episperms exhibited a better antioxidant activity demonstrated by ferric ion reducing antioxidant power, 2, 2′ - azino - bis (3-ethylbenzothiazoline -6-sulfonic acid diammonium salt, and 2, 2 -diphenyl -1-picrylhydrazyl radicals scavenging ability, and antibacterial against Escherichia coli, Salmonella, Staphyloccocus aureus, and Streptococcus, and anti-inflammatory activities in lipopolysaccharide induced RAW 264.7 cells. In a word, the present findings suggest that the total flavonoids fraction from Abutilon Theophrasti Medic. episperms was a very interesting candidate for the research and development of natural and healthy antioxidant, antibacterial, and anti-inflammatory agent for food, pharmaceutical, and medicine industries.
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the protective effect of the flavonoid fraction of Abutilon Theophrasti medic leaves on lps induced acute lung injury in mice via the nf κb and mapk signalling pathways
Biomedicine & Pharmacotherapy, 2019Co-Authors: Chunlian Tian, Peng Zhang, Hong Wang, Yuru Guo, Jie Yang, Zehui Zhang, Mingchun LiuAbstract:Abstract Accompanied by the damages of epithelial and capillary endothelial cell, acute lung injury is diagnosed with the typical pathological symptoms in clinic, including diffusing of pulmonary interstitial, alveolar oedema and hypoxic respiratory insufficiency. Current study focused on the investigation the anti-inflammatory action and mechanisms of total flavonoids extract (TFE) from Abutilon Theophrasti Medic. leaves on ALI mice induced by LPSs. Mice were administrated intragastrically with TFE at the concentrations of 0.25, 0.5, or 1.0 g/kg for 5 days, and on last day, nasal administration of LPSs for 6 h after 30 min for intragastric administration of TFE. Pretreatment with TFE not only reduced oxidative damage but also alleviated lung edema in ALI mice. Increased secretion of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, caused by LPSs was reversed by TFE; on the contrary, the anti-inflammatory cytokine IL-10 was upregulated. The proteins expressions of pro-inflammatory mediators iNOS and COX-2 induced by LPSs, were down-regulated by TFE. Moreover, the activation of NF-κB and MAPK signalling pathways was inhibited by TFE in LPSs induced ALI mice. The results revealed that the anti-inflammatory mechanisms of TFE were via inhibition of NF-κB and MAPK activation. Combined, the results suggested that TFE might exert in vivo antioxidant and anti-inflammatory functions in LPSs stimulated mice, and will be potential in adjuvant treatment in oxidative stress and inflammation diseases.
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Extraction Process, Component Analysis, and In Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon Theophrasti Medic. Leaves.
Mediators of inflammation, 2018Co-Authors: Chunlian Tian, Peng Zhang, Caixia Yang, Xiang Gao, Hong Wang, Yuru Guo, Mingchun LiuAbstract:The flavonoid fraction was extracted from the leaves of Abutilon Theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.
Yiqi Yang - One of the best experts on this subject based on the ideXlab platform.
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characterizing natural cellulose fibers from velvet leaf Abutilon Theophrasti stems
Bioresource Technology, 2008Co-Authors: Narendra Reddy, Yiqi YangAbstract:Velvet leaf (Abutilon Theophrasti) that is currently considered a weed and an agricultural problem could be used as a source for high quality natural cellulose fibers. The fibers obtained from the velvet leaf stems are mainly composed of approximately 69% cellulose and 17% lignin. The single cells in the fiber have lengths of approximately 0.9 mm, shorter than those in common bast fibers, hemp and kenaf. However, the widths of single cells in velvet leaf fibers are similar to the single cells in hemp and kenaf. The fibers exhibited breaking tenacity from 2.4 to 3.9 g/denier (325-500 MPa), breaking elongation of 1.6-2.4% and Young's modulus of 140-294 g/denier (18-38 GPa). Overall, velvet leaf fibers have properties similar to that of common bast fibers such as hemp and kenaf. Velvet leaves fibers could be processed on the current kenaf processing machineries for textile, composite, automotive and other fibrous applications.
