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Pitt Supaphol - One of the best experts on this subject based on the ideXlab platform.
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in vitro biological evaluation of electrospun cellulose Acetate Fiber mats containing asiaticoside or curcumin
Journal of Biomedical Materials Research Part A, 2010Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Pitt SupapholAbstract:Ultra-fine cellulose Acetate (CA; Mw ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing either asiaticoside [from the plant Centella asiatica (L.); either in the form of a crude extract (CACE) or pure substance (PAC)] or curcumin (CM; from the plant Curcuma longa L.) were successfully prepared. The proposed use of these materials is as topical/transdermal patches or wound dressings. Here, the potential for use of these herb-loaded CA Fiber mats as wound dressings was evaluated in terms of the stability and the antioxidant activity of the as-loaded herbal substances, the ability to support both the attachment and the proliferation of fibroblasts and the ability of the cultured fibroblasts to synthesize collagen. Normal human dermal fibroblasts (NHDF) were used as the reference fibroblastic cells. The results showed that the as-loaded herbal substances were stable even after the herb-loaded CA Fiber mats had been aged either at room temperature or at 40°C for a period of up to 4 months. The inclusion of asiaticoside [either 2% (w/w) CACE or 40% (w/w) PAC] rendered the resulting CA Fiber mats their superiority in supporting the attachment, promoting the proliferation, and upregulating the production of collagen of the seeded and/or the cultured NHDF to the corresponding solvent-cast films and the neat CA Fiber mats. On the other hand, the presence of CM imparted the antioxidant activity to the resulting CA Fiber mats. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
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development of polyelectrolyte multilayer coated electrospun cellulose Acetate Fiber mat as composite membranes
European Polymer Journal, 2008Co-Authors: Watadta Ritcharoen, Pitt Supaphol, Prasert PavasantAbstract:Abstract Electrostatic multilayers of chitosan (CHI)/sodium alginate (SA) and CHI/poly(styrene sulfonate) sodium salt (PSS) were alternatively coated on electrospun cellulose Acetate (CA) Fiber mat. Morphologies of the composite membranes were characterized by scanning electron microscopy. The morphology of the CHI/SA-coated membrane was denser than the CHI/PSS-coated one. The top layers consisted of carboxyl and sulfonic functional groups for SA and PSS layers, respectively. Amino groups of CHI were only presented in slight quantity. X-ray photoelectron spectroscopy (XPS) confirmed the deposition of the amino groups of CHI on the multilayer membrane surface. These composite membranes were characterized for its water permeability where the water flux decreased with an increase in the number of the bilayers. The water flux was in the range of 60 and 40 L m −2 h −1 for 15 and 25 bilayered membranes, respectively. The sodium chloride (NaCl) solution flux was lower than the pure water flux due to the effect of osmotic pressure, and it decreased with an increase in the NaCl concentration. The rejection of NaCl increased substantially with the number of the bilayers of the polyelectrolytes multilayers. The level of NaCl rejection from this work was in the range of 6% and 15% for 15 and 25 bilayered membranes, respectively.
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electrospun cellulose Acetate Fiber mats containing asiaticoside or centella asiatica crude extract and the release characteristics of asiaticoside
Polymer, 2008Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Pitt SupapholAbstract:Abstract Ultra-fine cellulose Acetate (CA; M w ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing asiaticoside (AC) from the plant Centella asiatica L. either in the form of pure substance (PAC) or a crude extract (CACE) were fabricated by electrospinning. Incorporation of either PAC or CACE (40 wt.% based on the weight of CA) in the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) did not affect the morphology of the obtained Fibers, as both the neat and the herb-loaded CA Fibers were smooth. The average diameters of these Fibers ranged between 301 and 545 nm. Determination of the release characteristics of AC from the herb-loaded CA Fiber mats was carried out by the total immersion and the transdermal diffusion through a pigskin method in Acetate or phosphate buffer solution that contained methanol (hereafter, A/B/M or P/B/M medium) at either 32 or 37 °C, respectively. In the total immersion method, the maximum amounts of the AC released from the PAC- and the CACE-loaded CA Fiber mats into the A/B/M medium were ∼24 and ∼10% (based on the weight of the specimens), while those of the AC released into the P/B/M medium were ∼26 and ∼12%, respectively. Considerably lower values were, however, obtained when the materials were placed on top of a piece of pigskin. Lastly, the herb-loaded CA Fiber mats released no substance that was harmful to normal human dermal fibroblasts, rending their potential for use as topical/transdermal or wound dressing patches.
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electrospun cellulose Acetate Fiber mats containing curcumin and release characteristic of the herbal substance
Polymer, 2007Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Praneet Opanasopit, Pitt SupapholAbstract:Abstract Ultra-fine cellulose Acetate (CA; M w ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing curcumin from the plant Curcuma longa L., widely known for its anti-tumor, antioxidant, and anti-inflammatory properties, were fabricated, for the first time, from the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) containing curcumin in various amounts (i.e., 5–20 wt.% based on the weight of CA powder) by electrospinning. Incorporation of curcumin in the neat CA solution did not affect the morphology of the resulting Fibers, as both the neat and the curcumin-loaded CA Fibers were smooth. The average diameters of the curcumin-loaded CA Fibers ranged between ∼314 and ∼340 nm. The integrity of the as-loaded curcumin in the curcumin-loaded CA Fiber mats was intact as indicated by the 1 H nuclear magnetic resonance spectrometric results and the ability of the as-loaded curcumin in maintaining its free radical scavenging ability. Investigation of the release characteristic of curcumin from the curcumin-loaded CA Fiber mats was carried out by the total immersion and the transdermal diffusion through a pig skin method in the Acetate buffer solution containing Tween 80 and methanol or the B/T/M medium at 37 °C. In the total immersion method, almost all of the curcumin loaded in the curcumin-loaded CA Fiber mat specimens was released into the medium (∼90 to ∼95%), while considerably lower values were obtained when the curcumin-loaded CA Fiber mats were placed on top of a piece of pig skin. Lastly, the curcumin-loaded CA Fiber mats were proven non-toxic to normal human dermal fibroblasts.
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release characteristics of four model drugs from drug loaded electrospun cellulose Acetate Fiber mats
Polymer, 2007Co-Authors: Santi Tungprapa, Ittipol Jangchud, Pitt SupapholAbstract:Abstract Ultra-fine Fiber mats of cellulose Acetate (CA; Mw ≈ 30 000 Da; degree of acetyl substitution ≈ 2.4) containing four different types of model drugs, i.e., naproxen (NAP), indomethacin (IND), ibuprofen (IBU), and sulindac (SUL), were successfully prepared by electrospinning from 16% w/v CA solutions in 2:1 v/v acetone/N,N-dimethylacetamide (DMAc). The amount of the drugs in the solutions was fixed at 20 wt.% based on the weight of CA powder. The morphology of the drug-loaded electrospun (e-spun) CA Fiber mats was smooth, with the average diameters of these Fibers ranging between 263 and 297 nm. No presence of the drug aggregates of any kind was observed on the surfaces of these Fibers, suggesting that the drugs were encapsulated well within the Fibers. After submersion in the Acetate buffer solution at 37 °C for 24 h, the drug-loaded e-spun CA Fiber mats swelled particularly well (i.e., 570–630%), while the corresponding solvent-cast film counterparts did not. The release characteristics of the model drugs from both the drug-loaded CA Fiber mats and the drug-loaded as-cast CA films were carried out by the total immersion method in the Acetate buffer solution at 37 °C. At any given immersion time point, the release of the drugs from the drug-loaded e-spun CA Fiber mats was greater than that from the corresponding as-cast films. The maximum release of the drugs from both the drug-loaded Fiber mats and films could be ranked as follows: NAP > IBU > IND > SUL.
Orawan Suwantong - One of the best experts on this subject based on the ideXlab platform.
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in vitro biological evaluation of electrospun cellulose Acetate Fiber mats containing asiaticoside or curcumin
Journal of Biomedical Materials Research Part A, 2010Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Pitt SupapholAbstract:Ultra-fine cellulose Acetate (CA; Mw ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing either asiaticoside [from the plant Centella asiatica (L.); either in the form of a crude extract (CACE) or pure substance (PAC)] or curcumin (CM; from the plant Curcuma longa L.) were successfully prepared. The proposed use of these materials is as topical/transdermal patches or wound dressings. Here, the potential for use of these herb-loaded CA Fiber mats as wound dressings was evaluated in terms of the stability and the antioxidant activity of the as-loaded herbal substances, the ability to support both the attachment and the proliferation of fibroblasts and the ability of the cultured fibroblasts to synthesize collagen. Normal human dermal fibroblasts (NHDF) were used as the reference fibroblastic cells. The results showed that the as-loaded herbal substances were stable even after the herb-loaded CA Fiber mats had been aged either at room temperature or at 40°C for a period of up to 4 months. The inclusion of asiaticoside [either 2% (w/w) CACE or 40% (w/w) PAC] rendered the resulting CA Fiber mats their superiority in supporting the attachment, promoting the proliferation, and upregulating the production of collagen of the seeded and/or the cultured NHDF to the corresponding solvent-cast films and the neat CA Fiber mats. On the other hand, the presence of CM imparted the antioxidant activity to the resulting CA Fiber mats. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
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electrospun cellulose Acetate Fiber mats containing asiaticoside or centella asiatica crude extract and the release characteristics of asiaticoside
Polymer, 2008Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Pitt SupapholAbstract:Abstract Ultra-fine cellulose Acetate (CA; M w ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing asiaticoside (AC) from the plant Centella asiatica L. either in the form of pure substance (PAC) or a crude extract (CACE) were fabricated by electrospinning. Incorporation of either PAC or CACE (40 wt.% based on the weight of CA) in the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) did not affect the morphology of the obtained Fibers, as both the neat and the herb-loaded CA Fibers were smooth. The average diameters of these Fibers ranged between 301 and 545 nm. Determination of the release characteristics of AC from the herb-loaded CA Fiber mats was carried out by the total immersion and the transdermal diffusion through a pigskin method in Acetate or phosphate buffer solution that contained methanol (hereafter, A/B/M or P/B/M medium) at either 32 or 37 °C, respectively. In the total immersion method, the maximum amounts of the AC released from the PAC- and the CACE-loaded CA Fiber mats into the A/B/M medium were ∼24 and ∼10% (based on the weight of the specimens), while those of the AC released into the P/B/M medium were ∼26 and ∼12%, respectively. Considerably lower values were, however, obtained when the materials were placed on top of a piece of pigskin. Lastly, the herb-loaded CA Fiber mats released no substance that was harmful to normal human dermal fibroblasts, rending their potential for use as topical/transdermal or wound dressing patches.
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electrospun cellulose Acetate Fiber mats containing curcumin and release characteristic of the herbal substance
Polymer, 2007Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Praneet Opanasopit, Pitt SupapholAbstract:Abstract Ultra-fine cellulose Acetate (CA; M w ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing curcumin from the plant Curcuma longa L., widely known for its anti-tumor, antioxidant, and anti-inflammatory properties, were fabricated, for the first time, from the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) containing curcumin in various amounts (i.e., 5–20 wt.% based on the weight of CA powder) by electrospinning. Incorporation of curcumin in the neat CA solution did not affect the morphology of the resulting Fibers, as both the neat and the curcumin-loaded CA Fibers were smooth. The average diameters of the curcumin-loaded CA Fibers ranged between ∼314 and ∼340 nm. The integrity of the as-loaded curcumin in the curcumin-loaded CA Fiber mats was intact as indicated by the 1 H nuclear magnetic resonance spectrometric results and the ability of the as-loaded curcumin in maintaining its free radical scavenging ability. Investigation of the release characteristic of curcumin from the curcumin-loaded CA Fiber mats was carried out by the total immersion and the transdermal diffusion through a pig skin method in the Acetate buffer solution containing Tween 80 and methanol or the B/T/M medium at 37 °C. In the total immersion method, almost all of the curcumin loaded in the curcumin-loaded CA Fiber mat specimens was released into the medium (∼90 to ∼95%), while considerably lower values were obtained when the curcumin-loaded CA Fiber mats were placed on top of a piece of pig skin. Lastly, the curcumin-loaded CA Fiber mats were proven non-toxic to normal human dermal fibroblasts.
Uracha Ruktanonchai - One of the best experts on this subject based on the ideXlab platform.
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in vitro biological evaluation of electrospun cellulose Acetate Fiber mats containing asiaticoside or curcumin
Journal of Biomedical Materials Research Part A, 2010Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Pitt SupapholAbstract:Ultra-fine cellulose Acetate (CA; Mw ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing either asiaticoside [from the plant Centella asiatica (L.); either in the form of a crude extract (CACE) or pure substance (PAC)] or curcumin (CM; from the plant Curcuma longa L.) were successfully prepared. The proposed use of these materials is as topical/transdermal patches or wound dressings. Here, the potential for use of these herb-loaded CA Fiber mats as wound dressings was evaluated in terms of the stability and the antioxidant activity of the as-loaded herbal substances, the ability to support both the attachment and the proliferation of fibroblasts and the ability of the cultured fibroblasts to synthesize collagen. Normal human dermal fibroblasts (NHDF) were used as the reference fibroblastic cells. The results showed that the as-loaded herbal substances were stable even after the herb-loaded CA Fiber mats had been aged either at room temperature or at 40°C for a period of up to 4 months. The inclusion of asiaticoside [either 2% (w/w) CACE or 40% (w/w) PAC] rendered the resulting CA Fiber mats their superiority in supporting the attachment, promoting the proliferation, and upregulating the production of collagen of the seeded and/or the cultured NHDF to the corresponding solvent-cast films and the neat CA Fiber mats. On the other hand, the presence of CM imparted the antioxidant activity to the resulting CA Fiber mats. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
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electrospun cellulose Acetate Fiber mats containing asiaticoside or centella asiatica crude extract and the release characteristics of asiaticoside
Polymer, 2008Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Pitt SupapholAbstract:Abstract Ultra-fine cellulose Acetate (CA; M w ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing asiaticoside (AC) from the plant Centella asiatica L. either in the form of pure substance (PAC) or a crude extract (CACE) were fabricated by electrospinning. Incorporation of either PAC or CACE (40 wt.% based on the weight of CA) in the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) did not affect the morphology of the obtained Fibers, as both the neat and the herb-loaded CA Fibers were smooth. The average diameters of these Fibers ranged between 301 and 545 nm. Determination of the release characteristics of AC from the herb-loaded CA Fiber mats was carried out by the total immersion and the transdermal diffusion through a pigskin method in Acetate or phosphate buffer solution that contained methanol (hereafter, A/B/M or P/B/M medium) at either 32 or 37 °C, respectively. In the total immersion method, the maximum amounts of the AC released from the PAC- and the CACE-loaded CA Fiber mats into the A/B/M medium were ∼24 and ∼10% (based on the weight of the specimens), while those of the AC released into the P/B/M medium were ∼26 and ∼12%, respectively. Considerably lower values were, however, obtained when the materials were placed on top of a piece of pigskin. Lastly, the herb-loaded CA Fiber mats released no substance that was harmful to normal human dermal fibroblasts, rending their potential for use as topical/transdermal or wound dressing patches.
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electrospun cellulose Acetate Fiber mats containing curcumin and release characteristic of the herbal substance
Polymer, 2007Co-Authors: Orawan Suwantong, Uracha Ruktanonchai, Praneet Opanasopit, Pitt SupapholAbstract:Abstract Ultra-fine cellulose Acetate (CA; M w ≈ 30,000 Da; degree of acetyl substitution ≈ 2.4) Fiber mats containing curcumin from the plant Curcuma longa L., widely known for its anti-tumor, antioxidant, and anti-inflammatory properties, were fabricated, for the first time, from the neat CA solution (17% w/v in 2:1 v/v acetone/dimethylacetamide) containing curcumin in various amounts (i.e., 5–20 wt.% based on the weight of CA powder) by electrospinning. Incorporation of curcumin in the neat CA solution did not affect the morphology of the resulting Fibers, as both the neat and the curcumin-loaded CA Fibers were smooth. The average diameters of the curcumin-loaded CA Fibers ranged between ∼314 and ∼340 nm. The integrity of the as-loaded curcumin in the curcumin-loaded CA Fiber mats was intact as indicated by the 1 H nuclear magnetic resonance spectrometric results and the ability of the as-loaded curcumin in maintaining its free radical scavenging ability. Investigation of the release characteristic of curcumin from the curcumin-loaded CA Fiber mats was carried out by the total immersion and the transdermal diffusion through a pig skin method in the Acetate buffer solution containing Tween 80 and methanol or the B/T/M medium at 37 °C. In the total immersion method, almost all of the curcumin loaded in the curcumin-loaded CA Fiber mat specimens was released into the medium (∼90 to ∼95%), while considerably lower values were obtained when the curcumin-loaded CA Fiber mats were placed on top of a piece of pig skin. Lastly, the curcumin-loaded CA Fiber mats were proven non-toxic to normal human dermal fibroblasts.
Nichoals P Chatterton - One of the best experts on this subject based on the ideXlab platform.
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ester prodrug loaded electrospun cellulose Acetate Fiber mats as transdermal drug delivery systems
Journal of Materials Science: Materials in Medicine, 2010Co-Authors: Christopher Branfordwhite, Limin Zhu, Nichoals P ChattertonAbstract:Cellulose Acetate (CA) Fibers loaded with the ester prodrugs of naproxen, including methyl ester, ethyl ester and isopropyl ester, were prepared through electrospinning using acetone/N,N-dimethylacetamide(DMAc)/ethanol (4:1:1, v/v/v) as solvent. The chemical and morphological characterizations of the medicated Fibers were investigated by means of SEM, DSC, XRD and FTIR, as well as the studies of the drug release properties. The results indicated that the morphology and diameter of the Fibers were influenced by the concentration of spinning solution, applied voltage, electrospun solvent and the surfactants. The average diameters of the Fibers ranged between 100 and 500 nm for three prodrugs. There was good compatibility between CA and three prodrugs in the blended Fibers, respectively. In vitro release indicated that constant drug release from the Fiber was observed over 6 days. The prodrugs were successfully encapsulated into the Fibers, and this system was stable in terms of effectiveness in release.
Christopher Branfordwhite - One of the best experts on this subject based on the ideXlab platform.
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ester prodrug loaded electrospun cellulose Acetate Fiber mats as transdermal drug delivery systems
Journal of Materials Science: Materials in Medicine, 2010Co-Authors: Christopher Branfordwhite, Limin Zhu, Nichoals P ChattertonAbstract:Cellulose Acetate (CA) Fibers loaded with the ester prodrugs of naproxen, including methyl ester, ethyl ester and isopropyl ester, were prepared through electrospinning using acetone/N,N-dimethylacetamide(DMAc)/ethanol (4:1:1, v/v/v) as solvent. The chemical and morphological characterizations of the medicated Fibers were investigated by means of SEM, DSC, XRD and FTIR, as well as the studies of the drug release properties. The results indicated that the morphology and diameter of the Fibers were influenced by the concentration of spinning solution, applied voltage, electrospun solvent and the surfactants. The average diameters of the Fibers ranged between 100 and 500 nm for three prodrugs. There was good compatibility between CA and three prodrugs in the blended Fibers, respectively. In vitro release indicated that constant drug release from the Fiber was observed over 6 days. The prodrugs were successfully encapsulated into the Fibers, and this system was stable in terms of effectiveness in release.
