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Elizabeth L.j. Watkin – One of the best experts on this subject based on the ideXlab platform.

  • Draft Genome Sequence of Acidihalobacter ferrooxidans DSM 14175 (Strain V8), a New Iron- and Sulfur-Oxidizing, Halotolerant, Acidophilic Species.
    Genome Announcements, 2017
    Co-Authors: Himel N. Khaleque, Joshua P. Ramsay, Riley J. T. Murphy, Anna H. Kaksonen, Naomi J. Boxall, Elizabeth L.j. Watkin

    Abstract:

    ABSTRACT The use of halotolerant Acidophiles for bioleaching provides a biotechnical approach for the extraction of metals from regions where high salinity exists in the ores and source water. Here, we describe the first draft genome of a new species of a halotolerant and iron- and sulfur-oxidizing Acidophile, Acidihalobacter ferrooxidans DSM-14175 (strain V8).

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  • RNA transcript sequencing reveals inorganic sulfur compound oxidation pathways in the Acidophile Acidithiobacillus ferrivorans
    FEMS Microbiology Letters, 2016
    Co-Authors: Stephan Christel, Elizabeth L.j. Watkin, Jimmy Fridlund, Antoine Buetti-dinh, Moritz Buck, Mark Dopson

    Abstract:

    Acidithiobacillus ferrivorans is an Acidophile implicated in low-temperature biomining for the recovery of metals from sulfide minerals. Acidithiobacillus ferrivorans obtains its energy from the ox …

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  • Bioleaching in brackish waters–effect of chloride ions on the Acidophile population and proteomes of model species.
    Applied Microbiology and Biotechnology, 2011
    Co-Authors: Carla M. Zammit, Mark Dopson, Stefanie Mangold, Venkateswara Rao Jonna, Lesley Mutch, Helen R. Watling, Elizabeth L.j. Watkin

    Abstract:

    High concentrations of chloride ions inhibit the growth of acidophilic microorganisms used in biomining, a problem particularly relevant to Western Australian and Chilean biomining operations. Despite this, little is known about the mechanisms Acidophiles adopt in order to tolerate high chloride ion concentrations. This study aimed to investigate the impact of increasing concentrations of chloride ions on the population dynamics of a mixed culture during pyrite bioleaching and apply proteomics to elucidate how two species from this mixed culture alter their proteomes under chloride stress. A mixture consisting of well-known biomining microorganisms and an enrichment culture obtained from an acidic saline drain were tested for their ability to bioleach pyrite in the presence of 0, 3.5, 7, and 20 g L−1 NaCl. Microorganisms from the enrichment culture were found to out-compete the known biomining microorganisms, independent of the chloride ion concentration. The proteomes of the Gram-positive Acidophile Acidimicrobium ferrooxidans and the Gram-negative Acidophile Acidithiobacillus caldus grown in the presence or absence of chloride ions were investigated. Analysis of differential expression showed that acidophilic microorganisms adopted several changes in their proteomes in the presence of chloride ions, suggesting the following strategies to combat the NaCl stress: adaptation of the cell membrane, the accumulation of amino acids possibly as a form of osmoprotectant, and the expression of a YceI family protein involved in acid and osmotic-related stress.

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David S Holmes – One of the best experts on this subject based on the ideXlab platform.

  • Effect of CO2 Concentration on Uptake and Assimilation of Inorganic Carbon in the Extreme Acidophile Acidithiobacillus ferrooxidans
    Frontiers in Microbiology, 2019
    Co-Authors: Mario Esparza, Mark Dopson, Eugenia Jedlicki, Carolina González, David S Holmes

    Abstract:

    This study was motivated by surprising gaps in the current knowledge of microbial inorganic carbon (Ci) uptake and assimilation at acidic pH values (pH < 3). Particularly striking is the limited understanding of the differences between Ci uptake mechanisms in acidic versus circumneutral environments where the Ci predominantly occurs either as a dissolved gas (CO2) or as bicarbonate (HCO3-), respectively. In order to gain initial traction on the problem, the relative abundance of transcripts encoding proteins involved in Ci uptake and assimilation was studied in the autotrophic, polyextreme Acidophile Acidithiobacillus ferrooxidans whose optimum pH for growth is 2.5 using ferrous iron as an energy source, although they are able to grow at pH 5 when using sulfur as an energy source. The relative abundance of transcripts of five operons (cbb1-5) and one gene cluster (can-sulP) was monitored by RT-qPCR and, in selected cases, at the protein level by Western blotting, when cells were grown under different regimens of CO2 concentration in elemental sulfur. Of particular note was the absence of a classical bicarbonate uptake system in A. ferrooxidans. However, bioinformatic approaches predict that sulP, previously annotated as a sulfate transporter, is a novel type of bicarbonate transporter. A conceptual model of CO2 fixation was constructed from combined bioinformatic and experimental approaches that suggests strategies for providing ecological flexibility under changing concentrations of CO2 and provides a portal to elucidating Ci uptake and regulation in acidic conditions. The results could advance the understanding of industrial bioleaching processes to recover metals such as copper at acidic pH. In addition, they may also shed light on how chemolithoautotrophic Acidophiles influence the nutrient and energy balance in naturally occurring low pH environments.

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  • Data_Sheet_4_Effect of CO2 Concentration on Uptake and Assimilation of Inorganic Carbon in the Extreme Acidophile Acidithiobacillus ferrooxidans.PDF
    , 2019
    Co-Authors: Mario Esparza, Mark Dopson, Eugenia Jedlicki, Carolina González, David S Holmes

    Abstract:

    This study was motivated by surprising gaps in the current knowledge of microbial inorganic carbon (Ci) uptake and assimilation at acidic pH values (pH < 3). Particularly striking is the limited understanding of the differences between Ci uptake mechanisms in acidic versus circumneutral environments where the Ci predominantly occurs either as a dissolved gas (CO2) or as bicarbonate (HCO3-), respectively. In order to gain initial traction on the problem, the relative abundance of transcripts encoding proteins involved in Ci uptake and assimilation was studied in the autotrophic, polyextreme Acidophile Acidithiobacillus ferrooxidans whose optimum pH for growth is 2.5 using ferrous iron as an energy source, although they are able to grow at pH 5 when using sulfur as an energy source. The relative abundance of transcripts of five operons (cbb1-5) and one gene cluster (can-sulP) was monitored by RT-qPCR and, in selected cases, at the protein level by Western blotting, when cells were grown under different regimens of CO2 concentration in elemental sulfur. Of particular note was the absence of a classical bicarbonate uptake system in A. ferrooxidans. However, bioinformatic approaches predict that sulP, previously annotated as a sulfate transporter, is a novel type of bicarbonate transporter. A conceptual model of CO2 fixation was constructed from combined bioinformatic and experimental approaches that suggests strategies for providing ecological flexibility under changing concentrations of CO2 and provides a portal to elucidating Ci uptake and regulation in acidic conditions. The results could advance the understanding of industrial bioleaching processes to recover metals such as copper at acidic pH. In addition, they may also shed light on how chemolithoautotrophic Acidophiles influence the nutrient and energy balance in naturally occurring low pH environments.

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  • Draft Genome Sequence of the Nominated Type Strain of “Ferrovum myxofaciens, ” an Acidophilic, Iron-Oxidizing Betaproteobacterium
    , 2016
    Co-Authors: Ana Moya-beltrán, David S Holmes, Juan Pablo A Cárdenas, Paulo B C. Covarrubias, Francisco B Issotta, Francisco A J. Oss, Barry A M. Grail, Raquel B Quatrini, B Barrie D. Johnsonc

    Abstract:

    “Ferrovummyxofaciens ” is an iron-oxidizing betaproteobacterium with widespread distribution in acidic low-temperature en-vironments, such as acid mine drainage streams. Here, we describe the genomic features of this novel Acidophile and investigate the relevant metabolic pathways that enable its survival in these environments

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Mark Dopson – One of the best experts on this subject based on the ideXlab platform.

  • Effect of CO2 Concentration on Uptake and Assimilation of Inorganic Carbon in the Extreme Acidophile Acidithiobacillus ferrooxidans
    Frontiers in Microbiology, 2019
    Co-Authors: Mario Esparza, Mark Dopson, Eugenia Jedlicki, Carolina González, David S Holmes

    Abstract:

    This study was motivated by surprising gaps in the current knowledge of microbial inorganic carbon (Ci) uptake and assimilation at acidic pH values (pH < 3). Particularly striking is the limited understanding of the differences between Ci uptake mechanisms in acidic versus circumneutral environments where the Ci predominantly occurs either as a dissolved gas (CO2) or as bicarbonate (HCO3-), respectively. In order to gain initial traction on the problem, the relative abundance of transcripts encoding proteins involved in Ci uptake and assimilation was studied in the autotrophic, polyextreme Acidophile Acidithiobacillus ferrooxidans whose optimum pH for growth is 2.5 using ferrous iron as an energy source, although they are able to grow at pH 5 when using sulfur as an energy source. The relative abundance of transcripts of five operons (cbb1-5) and one gene cluster (can-sulP) was monitored by RT-qPCR and, in selected cases, at the protein level by Western blotting, when cells were grown under different regimens of CO2 concentration in elemental sulfur. Of particular note was the absence of a classical bicarbonate uptake system in A. ferrooxidans. However, bioinformatic approaches predict that sulP, previously annotated as a sulfate transporter, is a novel type of bicarbonate transporter. A conceptual model of CO2 fixation was constructed from combined bioinformatic and experimental approaches that suggests strategies for providing ecological flexibility under changing concentrations of CO2 and provides a portal to elucidating Ci uptake and regulation in acidic conditions. The results could advance the understanding of industrial bioleaching processes to recover metals such as copper at acidic pH. In addition, they may also shed light on how chemolithoautotrophic Acidophiles influence the nutrient and energy balance in naturally occurring low pH environments.

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  • Data_Sheet_4_Effect of CO2 Concentration on Uptake and Assimilation of Inorganic Carbon in the Extreme Acidophile Acidithiobacillus ferrooxidans.PDF
    , 2019
    Co-Authors: Mario Esparza, Mark Dopson, Eugenia Jedlicki, Carolina González, David S Holmes

    Abstract:

    This study was motivated by surprising gaps in the current knowledge of microbial inorganic carbon (Ci) uptake and assimilation at acidic pH values (pH < 3). Particularly striking is the limited understanding of the differences between Ci uptake mechanisms in acidic versus circumneutral environments where the Ci predominantly occurs either as a dissolved gas (CO2) or as bicarbonate (HCO3-), respectively. In order to gain initial traction on the problem, the relative abundance of transcripts encoding proteins involved in Ci uptake and assimilation was studied in the autotrophic, polyextreme Acidophile Acidithiobacillus ferrooxidans whose optimum pH for growth is 2.5 using ferrous iron as an energy source, although they are able to grow at pH 5 when using sulfur as an energy source. The relative abundance of transcripts of five operons (cbb1-5) and one gene cluster (can-sulP) was monitored by RT-qPCR and, in selected cases, at the protein level by Western blotting, when cells were grown under different regimens of CO2 concentration in elemental sulfur. Of particular note was the absence of a classical bicarbonate uptake system in A. ferrooxidans. However, bioinformatic approaches predict that sulP, previously annotated as a sulfate transporter, is a novel type of bicarbonate transporter. A conceptual model of CO2 fixation was constructed from combined bioinformatic and experimental approaches that suggests strategies for providing ecological flexibility under changing concentrations of CO2 and provides a portal to elucidating Ci uptake and regulation in acidic conditions. The results could advance the understanding of industrial bioleaching processes to recover metals such as copper at acidic pH. In addition, they may also shed light on how chemolithoautotrophic Acidophiles influence the nutrient and energy balance in naturally occurring low pH environments.

    Free Register to Access Article

  • RNA transcript sequencing reveals inorganic sulfur compound oxidation pathways in the Acidophile Acidithiobacillus ferrivorans
    FEMS Microbiology Letters, 2016
    Co-Authors: Stephan Christel, Elizabeth L.j. Watkin, Jimmy Fridlund, Antoine Buetti-dinh, Moritz Buck, Mark Dopson

    Abstract:

    Acidithiobacillus ferrivorans is an Acidophile implicated in low-temperature biomining for the recovery of metals from sulfide minerals. Acidithiobacillus ferrivorans obtains its energy from the ox …

    Free Register to Access Article