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Aconite

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Shuying Liu – One of the best experts on this subject based on the ideXlab platform.

  • study on the metabolic characteristics of Aconite alkaloids in the extract of radix aconiti under intestinal bacteria of rat by uplc msn technique
    Chinese Journal of Chemistry, 2012
    Co-Authors: Yang Xin, Fengrui Song, Zhiqiang Liu, Shuying Liu

    Abstract:

    The extract of Radix aconiti was incubated with rat intestinal bacteria in vitro. Further, aconitine and hypaconitine standard were incubated at the same condition as the extract of Radix aconiti, respectively. Ultra Performance Liquid Chromatography/Multi-Mass Spectrometry (UPLC/MSn) was used for detecting and identifying all the Aconite alkaloids. Results showed that there were four metabolites which were identified as 8-butyryl-14-benzoylmesa-conine (m/z660), 8-propionyl-14-benzoylaconine (m/z660), 8-butyryl-14-benzoylaconine (m/z 674) and 8-valeryl-14-benzoylmesaconine (m/z674) in the metabolized sample of the extract of Radix aconiti. The relative area ratio of them presented increasing trend during 6 d. On the basis of all results, we could concluded that substitution at N atom mainly influenced the metabolizing rate of diester-diterpenoid alkaloids (DDAs), C-8 substitute was active metabolized site, intestinal bacterial metabolites of the Aconite alkaloids in the extract of Radix aconiti were mainly the substitute with propionyl group, butyl group or valeryl group at C-8. This paper illustrated holistic metabolizing profile of the extract of Radix aconiti in vitro and possible metabolizing reaction type of main DDAs, which could provide reference for finding out potential bioactive components in the extract and the prescription of Chinese Medicine.

  • analysis of Aconite alkaloids in the combination of radix aconiti lateralis preparata with different herbs by esi ms spectrometry
    Acta pharmaceutica Sinica, 2007
    Co-Authors: Hao Yue, Zhiqiang Liu, Fengrui Song, Shuying Liu

    Abstract:

    The method was established study the influence of different herbal combination with Radix Aconiti in the traditional medical formulae on content of the Aconite alkaloids, for elucidating the scientific basis of reducing the toxicity of Aconite in traditional Chinese medical formulation. The samples for ESI-MS study were prepared by decocting a mixture of Radix Aconiti Lateralis Preparata ( RALP) with Radix Glycyrrhizae Preparata (RGP) , Radix Paeoniae Alba ( RPA) , Rhizoma Zingiberis (RZ) or Radix Et Rhizoma Rhei ( RERR) , separately, and extracting the residue of the above mentioned mixtures after decocting. The diester-diterpenoid alkaloids (DDAs) was lower in the herb couples of RALP-RGP, RALP-RPA, RALP-RZ and RALP-RERR, and lipo-alkaloids was increased in the herb couples of RALP-RGP, RALP-RPA and RALP-RZ. The reason of reducing toxic effect principle is that the components of RGP, RPA and RZ have ester-exchange reactions with DDAs in RALP to produce lipo-alkaloids of low toxicity in the decocting process of the herb couples. The combination of RALP-RERR can reduce the content of DDAs in decoction and residue due to the formation of water insoluble alkaloid compound.

  • Study on the combination principles about Aconite roots by electrospray ionization mass spectrometry
    Acta pharmaceutica Sinica, 2003
    Co-Authors: Yong Wang, Zhiqiang Liu, Fengrui Song, Shuying Liu

    Abstract:

    Aim To explore the combination principle about Aconite roots from the chemical point of view. Methods Analyzing and comparing the nordeterpenoid alkaloids in the crude Aconite roots, the decoction of crude Aconite roots and Aconite roots with some other Chinese traditional herbs by electrospray ionization trap tandem mass spectrometry. Detecting the pH value of the decoction of crude Aconite roots and of some other Chinese traditional herbs; studying the influence of acidity to the hydrolysis of standard sample of mesaconitine. Results Most of diester-diterpenoid alkaloids in the decoction of Aconite roots and the decoction of Aconite roots with liquorice roots are hydrolyzed, and the hydrolysis of alkaloids are inhibited in the decoction of Aconite roots with Pinellia tuber or Schisandra. The acidity of the decoction of Aconite roots or liquorice is lower than the decoction of Pinellia tuber or Schisandra; standard sample of mesaconitine can not be hydrolyzed in acid water. Conclusion Acidity is a key factor to influence the hydrolysis of diester-diterpenoid alkaloids, and the hydrolysis of alkaloids are inhibited in high acidity, which lead to the high toxicity of the decoction containing Aconite roots.

Shepo Shi – One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of Aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra high performance liquid chromatography coupled with tandem mass spectrometry
    RSC Advances, 2015
    Co-Authors: Yuelin Song, Na Zhang, Yong Jiang, Yunfang Zhao, Shepo Shi

    Abstract:

    Aconite alkaloids and ginsenosides have been demonstrated as the effective constituents in Shenfu injection (SFI), which is a widely used Chinese herbal formulation prepared from red ginseng and processed Aconite root. Quality control is quite critical to meet the increasing demand of SFI in the market and to guarantee safety in clinics, in particular regarding the toxic Aconite alkaloids. Given the significant merits of online solid phase extraction (SPE) for sample preparation, an online SPE-based method was developed to simultaneously quantify ten Aconite alkaloids and thirteen ginsenosides in SFI using UHPLC-MS/MS. Because of their distinct ionization behaviors, polarity switching was implemented in the ion source domain with a scheduled program, and positive and negative ionization modes were applied for alkaloids and saponins, respectively. Quantitative terms of the proposed method with respect to linearity, limit of detection, lower limit of quantification, precision, and accuracy were evaluated, and the results indicated that the method is sensitive, convenient, and reliable. The developed method was successfully applied for the simultaneous determination of Aconite alkaloids and ginsenosides in ten batches of SFI (SFI1-10). The validated method can be adopted as a meaningful tool for the quality control of SFI concerning Aconite alkaloids and ginsenosides, and also it offers a reliable choice for the widely qualitative analysis of constituents in complex matrices being free from tedious sample preparation procedures.

Yuelin Song – One of the best experts on this subject based on the ideXlab platform.

  • simultaneous determination of Aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra high performance liquid chromatography coupled with tandem mass spectrometry
    RSC Advances, 2015
    Co-Authors: Yuelin Song, Na Zhang, Yong Jiang, Yunfang Zhao, Shepo Shi

    Abstract:

    Aconite alkaloids and ginsenosides have been demonstrated as the effective constituents in Shenfu injection (SFI), which is a widely used Chinese herbal formulation prepared from red ginseng and processed Aconite root. Quality control is quite critical to meet the increasing demand of SFI in the market and to guarantee safety in clinics, in particular regarding the toxic Aconite alkaloids. Given the significant merits of online solid phase extraction (SPE) for sample preparation, an online SPE-based method was developed to simultaneously quantify ten Aconite alkaloids and thirteen ginsenosides in SFI using UHPLC-MS/MS. Because of their distinct ionization behaviors, polarity switching was implemented in the ion source domain with a scheduled program, and positive and negative ionization modes were applied for alkaloids and saponins, respectively. Quantitative terms of the proposed method with respect to linearity, limit of detection, lower limit of quantification, precision, and accuracy were evaluated, and the results indicated that the method is sensitive, convenient, and reliable. The developed method was successfully applied for the simultaneous determination of Aconite alkaloids and ginsenosides in ten batches of SFI (SFI1-10). The validated method can be adopted as a meaningful tool for the quality control of SFI concerning Aconite alkaloids and ginsenosides, and also it offers a reliable choice for the widely qualitative analysis of constituents in complex matrices being free from tedious sample preparation procedures.