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Shuying Liu – One of the best experts on this subject based on the ideXlab platform.

  • Quantitative Assessment of the Influence of Rhizoma Zingiberis on the Level of Aconitine in Rat Gut Sacs and Qualitative Analysis of the Major Influencing Components of Rhizoma Zingiberis on Aconitine Using UPLC/MS.
    PloS one, 2015
    Co-Authors: Yang Xin, Shuying Liu

    Abstract:

    This study attempted to clarify the material basis for the detoxification of Rhizoma Zingiberis (RZ) on Aconitine, an analgesic drug, by quantitatively assessing the influence of RZ on the in vitro intestinal concentration of Aconitine using an everted gut sac model and by qualitatively identifying the components in the RZ extract. To quantify Aconitine in rat everted gut sacs, both an accurate processing method and a sensitive detection method were required. We developed a three-step sample processing method to protect the components from decomposition and applied ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC/TQMS) to quantify Aconitine, glucose and digoxin. In addition, ultra-performance liquid chromatography coupled with linear ion trap mass spectrometry (UPLC/ITMS) was applied to detect the potential antidotal components in the RZ extract. Finally, the RZ extract reduced the level of Aconitine in everted gut sacs, and eleven gingerols were successfully identified, which could be considered potential antidotal components for Aconitine. This study demonstrated the application of two UPLC/MS methods for analyzing the material basis for the reciprocity between Chinese medicine components in everted gut sacs.

  • characterization of Aconitine type alkaloids in the flowers of aconitum kusnezoffii by electrospray ionization tandem mass spectrometry
    Journal of Mass Spectrometry, 2003
    Co-Authors: Yong Wang, Fengrui Song, Zhiqiang Liu, Shuying Liu

    Abstract:

    The fragmentation mechanism of Aconitine-type alkaloids in the flowers of Aconitum kusnezoffii (FAK) was investigated using electrospray ionization tandem mass spectrometry (ESI-MSn) firstly. The analysis of the collision-induced dissociation (CID) spectra of three purified Aconitine standards and six previously reported Aconitines indicated that the fragmentation of the protonated Aconitines at low-energy CID follows a similar pathway. The elimination of a C-8-substituent such as an acetic acid or a fatty acid is the dominant fragmentation mode in MS2. Successive losses of CH3COOH, CH3OH, H2O, BzOH, and CO are the main fragmentation pathways of Aconitine-type alkaloids in MS3 spectra. Based on these features, a rapid method for the direct detection and characterization of alkaloids from an ethanolic extract of FAK is described. All the known aconitum alkaloids are detected and a series of lipo-Aconitines has been found for the first time in this plant.

  • exploring the ester exchange reactions of diester diterpenoid alkaloids in the aconite decoction process by electrospray ionization tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Yong Wang, Lei Shi, Fengrui Song, Zhiqiang Liu, Shuying Liu

    Abstract:

    The chemical components in the decoctions of Chinese herbal medicines are not always the same as those in the crude herbs because of the insolubility or instability of some compounds. In this work electrospray ionization tandem mass spectrometry was used to explore the ester-exchange reactions for Aconitine-type diester-diterpenoid alkaloids occurring during the process of decocting aconite root. The Aconitines were screened in a diverse range of samples, including crude aconite, decoction of crude aconite, residues from decoction of crude aconite, prepared aconite, decoction of prepared aconite, decoction of prepared aconite with added palmitic acid, and decoction of a mixture of mesAconitine and hypAconitine standards with liquorice root. It was found that diester-diterpenoid Aconitines were converted into lipo-alkaloids as well as monoester alkaloids by the decoction of aconite.

Yong Wang – One of the best experts on this subject based on the ideXlab platform.

  • characterization of Aconitine type alkaloids in the flowers of aconitum kusnezoffii by electrospray ionization tandem mass spectrometry
    Journal of Mass Spectrometry, 2003
    Co-Authors: Yong Wang, Fengrui Song, Zhiqiang Liu, Shuying Liu

    Abstract:

    The fragmentation mechanism of Aconitine-type alkaloids in the flowers of Aconitum kusnezoffii (FAK) was investigated using electrospray ionization tandem mass spectrometry (ESI-MSn) firstly. The analysis of the collision-induced dissociation (CID) spectra of three purified Aconitine standards and six previously reported Aconitines indicated that the fragmentation of the protonated Aconitines at low-energy CID follows a similar pathway. The elimination of a C-8-substituent such as an acetic acid or a fatty acid is the dominant fragmentation mode in MS2. Successive losses of CH3COOH, CH3OH, H2O, BzOH, and CO are the main fragmentation pathways of Aconitine-type alkaloids in MS3 spectra. Based on these features, a rapid method for the direct detection and characterization of alkaloids from an ethanolic extract of FAK is described. All the known aconitum alkaloids are detected and a series of lipo-Aconitines has been found for the first time in this plant.

  • exploring the ester exchange reactions of diester diterpenoid alkaloids in the aconite decoction process by electrospray ionization tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Yong Wang, Lei Shi, Fengrui Song, Zhiqiang Liu, Shuying Liu

    Abstract:

    The chemical components in the decoctions of Chinese herbal medicines are not always the same as those in the crude herbs because of the insolubility or instability of some compounds. In this work electrospray ionization tandem mass spectrometry was used to explore the ester-exchange reactions for Aconitine-type diester-diterpenoid alkaloids occurring during the process of decocting aconite root. The Aconitines were screened in a diverse range of samples, including crude aconite, decoction of crude aconite, residues from decoction of crude aconite, prepared aconite, decoction of prepared aconite, decoction of prepared aconite with added palmitic acid, and decoction of a mixture of mesAconitine and hypAconitine standards with liquorice root. It was found that diester-diterpenoid Aconitines were converted into lipo-alkaloids as well as monoester alkaloids by the decoction of aconite.

  • electrospray ionization tandem mass spectrometric study of the Aconitines in the roots of aconite
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Yong Wang, Fengrui Song, Zhiqiang Liu, Shuying Liu

    Abstract:

    Fragmentation pathways of Aconitine-type alkaloids were investigated by electrospray ionization/ion trap multistage tandem mass spectrometry. Low-energy collision-induced dissociation of protonated Aconitines follows a dominant first step, the elimination of the C-8-substituent as acetic acid or fatty acid in MS2 spectra. Successive losses of 1-4 CH3OH molecules, 1-3 H2O, CO, benzoic acid, and CH3 or C2H5 (N-substituents) are all fragmentation pathways observed in MS3 and MS4 spectra. By applying knowledge of these fragmentation pathways to the Aconitines in the ethanolic extract of aconite roots, all the known Aconitines were detected and also 23 unknown Aconitine-type alkaloids, in which the lipo-alkaloids containing residues of 15C, 17C and 19C saturated or unsaturated fatty acids were characterized. These odd-carbon-number fatty acid substituents have not been reported previously.

Xianju Huang – One of the best experts on this subject based on the ideXlab platform.

  • sweroside alleviated Aconitine induced cardiac toxicity in h9c2 cardiomyoblast cell line
    Frontiers in Pharmacology, 2018
    Co-Authors: You Yu, Hui Chen, Mei Li, Awais Ihsan, Haiying Tong, Xianju Huang

    Abstract:

    Aconitine is the main bioactive ingredient of Aconitum plants, which are well-known botanical herbs in China. Aconitine is also notorious for its high cardiotoxicity, as it can induce life-threatening ventricular arrhythmias. Unfortunately, there are few effective antidotes to Aconitine toxicity. This study aimed to evaluate the potent protective effects of the ingredients from V. baillonii on Aconitine toxicity on H9c2 cell line. Cell viability was assessed by methylthiazoltetrazolium bromide (MTT). Intracellular Ca2+ concentration alteration and reactive oxygen species (ROS) generation were observed by confocal microscopy and flow cytometry, respectively. Cellular oxidative stress was analyzed by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Mitochondrial membrane potential (ΔΨ) was determined using JC-1 kit. RT-PCR and Hoechst staining techniques were conducted to determine the levels of autophagy/apoptosis. The mRNA levels of dihydropyridine receptor (DHPR), ryanodine receptors (RyR2) and sarcoplasmic reticulum Ca2+-ATPase (SERCA) were measured by RT-PCR. We screened six components from V. baillonii, among which, sweroside exhibited the strongest protective effects on Aconitine-induced cardiac toxicity. Sweroside suppressed the Aconitine-induced mRNA expressions of NaV1.5 (encoded by SCN5A), RyR2 and DHPR, and reversed the Aconitine-induced decrease in mRNA level of SERCA, thus preventing the Aconitine-induced persistent intracellular Ca2+ accumulation and avoiding intracellular Ca2+ overload. We further found that sweroside restabilized the Aconitine-disrupted mitochondrial membrane potential (ΔΨ) and reversed the Aconitine-induced increase in the mRNA levels of cell autophagy-related factors (Beclin-1, Caspase-3, and LC3- II) in H9c2 cells. In the whole-animal experiments, we observed that sweroside (50 mg/kg) alleviated effectively Aconitine-induced arrhythmias by analysis of electrocardiogram (ECG) recording in rats. Our results demonstrate that sweroside may protect cardiomyocytes from Aconitine toxicity by maintaining intracellular Ca2+ homeostasis, restabilizing mitochondrial membrane potential (ΔΨ) and avoiding cell autophagy/apoptosis.

  • sweroside alleviated Aconitine induced cardiac toxicity in h9c2 cardiomyoblast cell line
    Frontiers in Pharmacology, 2018
    Co-Authors: You Yu, Hui Chen, Mei Li, Awais Ihsan, Haiying Tong, Xianju Huang

    Abstract:

    Aconitine is the main bioactive ingredient of Aconitum plants, which are well-known botanical herbs in China. Aconitine is also notorious for its high cardiotoxicity, as it can induce life-threatening ventricular arrhythmias. Unfortunately, there are few effective antidotes to Aconitine toxicity. This study aimed to evaluate the potent protective effects of the ingredients from V. baillonii on Aconitine toxicity on H9c2 cell line. Cell viability was assessed by methylthiazoltetrazolium bromide (MTT). Intracellular Ca2+ concentration alteration and reactive oxygen species (ROS) generation were observed by confocal microscopy and flow cytometry, respectively. Cellular oxidative stress was analyzed by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Mitochondrial membrane potential (ΔΨ) was determined using JC-1 kit. RT-PCR and Hoechst staining techniques were conducted to determine the levels of autophagy/apoptosis. The mRNA levels of dihydropyridine receptor (DHPR), ryanodine receptors (RyR2) and sarcoplasmic reticulum Ca2+-ATPase (SERCA) were measured by RT-PCR. We screened six components from V. baillonii, among which, sweroside exhibited the strongest protective effects on Aconitine-induced cardiac toxicity. Sweroside suppressed the Aconitine-induced mRNA expressions of NaV1.5 (encoded by SCN5A), RyR2 and DHPR, and reversed the Aconitine-induced decrease in mRNA level of SERCA, thus preventing the Aconitine-induced persistent intracellular Ca2+ accumulation and avoiding intracellular Ca2+ overload. We further found that sweroside restabilized the Aconitine-disrupted mitochondrial membrane potential (ΔΨ) and reversed the Aconitine-induced increase in the mRNA levels of cell autophagy-related factors (Beclin-1, Caspase-3, and LC3- II) in H9c2 cells. In the whole-animal experiments, we observed that sweroside (50 mg/kg) alleviated effectively Aconitine-induced arrhythmias by analysis of electrocardiogram (ECG) recording in rats. Our results demonstrate that sweroside may protect cardiomyocytes from Aconitine toxicity by maintaining intracellular Ca2+ homeostasis, restabilizing mitochondrial membrane potential (ΔΨ) and avoiding cell autophagy/apoptosis.