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Actin Antibody

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Rufus W. Burlingame – One of the best experts on this subject based on the ideXlab platform.

Peter E. Braun – One of the best experts on this subject based on the ideXlab platform.

Yunching Huang – One of the best experts on this subject based on the ideXlab platform.

Francisco J B Sampaio – One of the best experts on this subject based on the ideXlab platform.

Guifang Wang – One of the best experts on this subject based on the ideXlab platform.

  • prominent expression of phosphodiesterase 5 in striated muscle of the rat urethra and levator ani
    The Journal of Urology, 2010
    Co-Authors: Yunching Huang, Guifang Wang
    Abstract:

    Purpose: We investigated phosphodiesterase 5 distribution and activity in the urethra.Materials and Methods: Rat tissues were examined for phosphodiesterase 5 and α-smooth muscle Actin expression. Urethral phosphodiesterase 5 activity was examined by tissue bath in the presence of sildenafil (Pfizer, New York, New York).Results: Anti-α-smooth muscle Actin Antibody (Abcam®) stained all known smooth muscles in all tested tissues and revealed a few smooth muscle fibers in the levator ani muscle. Anti-phosphodiesterase 5 Antibody (Abcam) stained smooth muscle in the penis and bladder but not striated leg muscle. However, it stained predominantly striated muscle in the urethra and the levator ani muscle. In the urethra the amount of phosphodiesterase 5 in striated muscle was 6 times that in smooth muscle. In urethral striated muscle phosphodiesterase 5 expression was localized to Z-band striations. Smooth and striated muscle intermingling was clearly visible on the inner and outer rims of the circularly arrang…