The Experts below are selected from a list of 3657 Experts worldwide ranked by ideXlab platform
P Fito - One of the best experts on this subject based on the ideXlab platform.
-
analysis of chemical and structural changes in kiwifruit Actinidia deliciosa cv hayward through the osmotic dehydration
Journal of Food Engineering, 2011Co-Authors: Marta Castrogiraldez, Urszula Tylewicz, P Fito, Dalla M RosaAbstract:Abstract Osmotic dehydration experiments of kiwifruit ( Actinidia deliciosa cv Hayward ) were carried out in order to apply a nonlinear irreversible thermodynamic model. Samples were immersed into 65% (w/w) sucrose aqueous solution at 30 °C during 5, 10, 15, 20, 30, 45, 60, 90, 120, 180, 250, 320, 400, 720, 1440 min. Some physical–chemical parameters were measured in fresh, treated and reposed (24 h at 30 °C) samples. It was possible to apply the enthalpy–entropy compensation coupled to a nonlinear thermodynamic model, obtaining the apparent bulk modulus and explaining the elastic answer of the tissue throughout the osmotic process. The osmotic dehydration also produces losses in the native compounds of kiwifruit such as citric acid and Calcium and Potassium.
D Mariotti - One of the best experts on this subject based on the ideXlab platform.
-
increase of rooting ability in the woody species kiwi Actinidia deliciosa a chev by transformation with agrobacterium rhizogenes rol genes
Plant Cell Reports, 1991Co-Authors: Eddo Rugini, Alessandro Pellegrineschi, Massimo Mencuccini, D MariottiAbstract:The woody species kiwi (Actinidia deliciosa A. Chev.), a male and late flowering clone of the cv Hayward, has been transformed by a T-DNA fragment encompassing rol A, B, C genes of A. rhizogenes. Transgenic plants, regenerated from leaf disc callus, showed the typical “hairy root” phenotype as described in herbaceous species. Explants from these plants (both leaf discs or 3 to 4 node leafy microcuttings) showed an increased ability to produce roots. Since root formation is one of the limiting factors in the vegetative propagation of woody species, the results have been discussed in relation to the use of A. rhizogenes rol genes in improving root morphogenesis in trees.
M. Salomé S. Pais - One of the best experts on this subject based on the ideXlab platform.
-
Somatic embryogenesis in leaves and leaf-derived protoplasts of Actinidia deliciosa var. deliciosa cv. Hayward (kiwifruit)
Plant Cell Reports, 1992Co-Authors: M. Margarida Oliveira, M. Salomé S. PaisAbstract:The obtention of embryogenic competence in Actinidia deliciosa var. deliciosa cv. Hayward is reported. Axillary buds from shoots submitted to cold (4°C) and starvation for 1.5 months, developed leaves with embryogenic competence. These leaves, cultured in darkness for 1.5 months on a medium containing zeatin as a sole growth regulator, originated compact structures from which embryos developed. The plating orientation and sectioning of leaves strongly affected the expression of the embryogenic potential. A selected fraction of the protoplasts isolated from these leaves was able to develop in an embryogenic way. The germination of the embryos is still only occasional.
-
Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit).
Plant Cell Reports, 1991Co-Authors: M. Margarida Oliveira, M. Salomé S. PaisAbstract:Plant regeneration of Actinidia deliciosa var. deliciosa cv. Hayword was obtained from protoplasts isolated from petiole derived long-term callus cultures. Protoplasts were cultured in liquid medium over agarose gelled medium. Regenerated green callus, plated on solid medium, could develop shoots that rooted spontaneously in hormone-less medium. The plants obtained are growing fast in soil and present a normal phenotype.
Asma Nasib - One of the best experts on this subject based on the ideXlab platform.
-
OPTIMIZATION AND ESTABLISHMENT OF Actinidia deliciosa CELL SUSPENSION CULTURE TO STUDY THE BIOTRANSFORMATION FOR THE PRODUCTION OF NOVEL COMPOUNDS
Pakistan Journal of Botany, 2020Co-Authors: Asma Nasib, Saifullah KhanAbstract:A highly efficient protocol for the induction of callus and establishment of cell suspension culture was developed for Actinidia deliciosa. Germination of Actinidia seeds were carried out on full strength MS medium without any growth hormone and the leaves from In vitro grown plantlets were used for the induction of callus. Maximum amount of friable calli were obtained on full strength MS medium supplemented with 1.0 mg/L IBA (Indole-3-butyric acid) and 0.5 mg/L BAP (6-Benzyl amino purine) with the 16 hrs illumination period. Cell suspension cultures were established using MS medium with higher amount of IBA (2.0 mg/L) and BAP (0.5 mg/L), by rotating the culture flasks at 110 rpm on a gyratory shaker. Biotransformation ability of the suspension culture of Actinidia deliciosa was also determined by adding (-)-Ambrox to the cultures as a substrate. Six transforms were isolated after 15 days of incubation while two of them were found novel.
-
an optimized and improved method for the in vitro propagation of kiwifruit Actinidia deliciosa using coconut water
2008Co-Authors: Asma Nasib, Saifullah KhanAbstract:Kiwifruit (Actinidia deliciosa) is grown for its excellent food and nutritional value in many parts of the world. The main objective of this study was to optimize an efficient, reliable and economical protocol for In vitro micropropagation of Kiwifruit. The effect of coconut water along with BAP on shoot proliferation of Kiwifruit (Actinidia deliciosa) has been evaluated. It has been noticed that both the BAP and coconut water had a synergistic effect and none of them was found able to generate the maximum response when used separately. Maximum shoot length (7.2 ± 0.16), number of shoots (11.5 ± 1.5) and number of nodes (4.6 ± 0.22) were achieved on the MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10). The use of coconut water also resulted in the longer sub-culturing time and the production of highly robust plants which were able to survive in the green house conditions. The proliferated shoots were subjected to root induction and half strength MS media with 0.2 mg/L IBA was found optimum for the root formation. The sufficiently rooted plantlets were transferred to green house for hardening. The potting mix comprised of 90% sand and 10% farm yard manure (v/v) and more than 95% of the plants, subjected to acclimatization, survived under green house conditions.
-
biotransformation of ambrox by cell suspension cultures of Actinidia deliciosa
Journal of Natural Products, 2006Co-Authors: Asma Nasib, Syed Ghulam Musharraf, Sajjad Hussain, Saifullah Khan, Shazia Anjum, Attaurrahman Attaurrahman, Iqbal M ChoudharyAbstract:Biotransformation of (−)-ambrox (1) with cell suspension cultures of Actinidia deliciosa (Kiwifruit) yielded the regio- and stereospecific oxygenated products 3-oxoambrox (2), 3β-hydroxyambrox (3), 1α-hydroxyambrox (4), 3β,6β-dihydroxyambrox (5), 1α,6β-dihydroxyambrox (6), and 1α,3β-dihydroxyambrox (7). Metabolites 6 and 7 were found to be new compounds. These metabolites were structurally characterized on the basis of spectroscopic studies. The structure of compound 6 was unambiguously deduced by single-crystal X-ray diffraction techniques. Metabolites 2−7 were evaluated for in vitro inhibitory activity against the thymidine phosphorylase enzyme.
Saifullah Khan - One of the best experts on this subject based on the ideXlab platform.
-
OPTIMIZATION AND ESTABLISHMENT OF Actinidia deliciosa CELL SUSPENSION CULTURE TO STUDY THE BIOTRANSFORMATION FOR THE PRODUCTION OF NOVEL COMPOUNDS
Pakistan Journal of Botany, 2020Co-Authors: Asma Nasib, Saifullah KhanAbstract:A highly efficient protocol for the induction of callus and establishment of cell suspension culture was developed for Actinidia deliciosa. Germination of Actinidia seeds were carried out on full strength MS medium without any growth hormone and the leaves from In vitro grown plantlets were used for the induction of callus. Maximum amount of friable calli were obtained on full strength MS medium supplemented with 1.0 mg/L IBA (Indole-3-butyric acid) and 0.5 mg/L BAP (6-Benzyl amino purine) with the 16 hrs illumination period. Cell suspension cultures were established using MS medium with higher amount of IBA (2.0 mg/L) and BAP (0.5 mg/L), by rotating the culture flasks at 110 rpm on a gyratory shaker. Biotransformation ability of the suspension culture of Actinidia deliciosa was also determined by adding (-)-Ambrox to the cultures as a substrate. Six transforms were isolated after 15 days of incubation while two of them were found novel.
-
an optimized and improved method for the in vitro propagation of kiwifruit Actinidia deliciosa using coconut water
2008Co-Authors: Asma Nasib, Saifullah KhanAbstract:Kiwifruit (Actinidia deliciosa) is grown for its excellent food and nutritional value in many parts of the world. The main objective of this study was to optimize an efficient, reliable and economical protocol for In vitro micropropagation of Kiwifruit. The effect of coconut water along with BAP on shoot proliferation of Kiwifruit (Actinidia deliciosa) has been evaluated. It has been noticed that both the BAP and coconut water had a synergistic effect and none of them was found able to generate the maximum response when used separately. Maximum shoot length (7.2 ± 0.16), number of shoots (11.5 ± 1.5) and number of nodes (4.6 ± 0.22) were achieved on the MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10). The use of coconut water also resulted in the longer sub-culturing time and the production of highly robust plants which were able to survive in the green house conditions. The proliferated shoots were subjected to root induction and half strength MS media with 0.2 mg/L IBA was found optimum for the root formation. The sufficiently rooted plantlets were transferred to green house for hardening. The potting mix comprised of 90% sand and 10% farm yard manure (v/v) and more than 95% of the plants, subjected to acclimatization, survived under green house conditions.
-
biotransformation of ambrox by cell suspension cultures of Actinidia deliciosa
Journal of Natural Products, 2006Co-Authors: Asma Nasib, Syed Ghulam Musharraf, Sajjad Hussain, Saifullah Khan, Shazia Anjum, Attaurrahman Attaurrahman, Iqbal M ChoudharyAbstract:Biotransformation of (−)-ambrox (1) with cell suspension cultures of Actinidia deliciosa (Kiwifruit) yielded the regio- and stereospecific oxygenated products 3-oxoambrox (2), 3β-hydroxyambrox (3), 1α-hydroxyambrox (4), 3β,6β-dihydroxyambrox (5), 1α,6β-dihydroxyambrox (6), and 1α,3β-dihydroxyambrox (7). Metabolites 6 and 7 were found to be new compounds. These metabolites were structurally characterized on the basis of spectroscopic studies. The structure of compound 6 was unambiguously deduced by single-crystal X-ray diffraction techniques. Metabolites 2−7 were evaluated for in vitro inhibitory activity against the thymidine phosphorylase enzyme.
