The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Michael Goodfellow - One of the best experts on this subject based on the ideXlab platform.
-
Actinomadura alkaliterrae sp. nov., isolated from an alkaline soil
Antonie van Leeuwenhoek, 2017Co-Authors: Imen Nouioui, Michael Goodfellow, H-p Klenk, Nevzat Sahin, Maria Carmen Montero-calasanz, Lorena Carro, José M. Igual, Demet Çetin, Kamil IşıkAbstract:A polyphasic study was undertaken to establish the taxonomic status of an Actinomadura strain isolated from the margin of a saline, alkaline lake in Central Anatolia, Turkey. Strain D310AT^T was shown to have chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Actinomadura such as hooked or irregular spiral spore chains, meso -diaminopimelic acid as the major cell wall diaminopimelic acid, and diphosphatidylglycerol and phosphatidylinositol as major polar lipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain D310AT^T is closely, albeit loosely, associated with Actinomadura darangshiensis DLS-70^T with 97.2% sequence similarity, but was readily separated from the latter using diverse phenotypic properties. Consequently, the isolate is considered to represent a new species of Actinomadura for which the name Actinomadura alkaliterrae sp. nov. is proposed, with the type strain D310AT^T (=DSM 101185^T = KCTC 39657^T).
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
-
Numerical phenetic classification of clinically significant aerobic sporoactinomycetes and related organisms
Antonie van Leeuwenhoek, 2003Co-Authors: Martha E. Trujillo, Michael GoodfellowAbstract:Clinically significant aerobic sporoactinomycetes, notably agents of mycetoma, were examined for a balanced set of unit characters and the resultant data analysed using standard numerical taxonomic procedures. All save two of the one hundred and seventy three tested strains were assigned to three multimembered cluster-groups, which encompassed sixteen major (4–7 strains), ten minor (2–3 strains) and forty single membered clusters, in an analysis based on the simple matching coefficient and unweighted pair group method with arithmetic averages algorithm. The three cluster-groups were equated with the genus Actinomadura (including Actinocorallia and Streptomyces somaliensis strains), and the genera Nocardiopsis and Streptomyces , and Thermobifida and Thermomonospora , respectively. In a corresponding principal co-ordinates analysis four multimembered groups corresponding to the genera Actinomadura , Nocardiopsis , Streptomyces , and Thermobifida and Thermomonospora were recognised. The causal agents of actinomycetoma were not only assigned to established taxa, notably, to Actinomadura latina , Actinomadura madurae , Actinomadura pelletieri and Streptomyces somaliensis , but also to additional centres of taxonomic variation which were equated with the rank of species. Most of the streptomycetes isolated from clinical material were assigned to clusters equated with the species Streptomyces albus and Streptomyces anulatus . The numerical taxonomic data were used to generate a frequency matrix designed to facilitate the identification of clinically significant Actinomadura , Nocardiopsis and Streptomyces strains to the species level; rapid enzyme tests accounted for eleven out of the twenty-one diagnostic tests.
-
Actinomadura mexicana sp. nov. and Actinomadura meyerii sp. nov., Two Novel Soil Sporoactinomycetes
Systematic and applied microbiology, 2003Co-Authors: Erika T. Quintana, Martha E. Trujillo, Michael GoodfellowAbstract:Summary The taxonomic position of two soil isolates, strains A288 T and A290 T [provisionally assigned to the genus Actinomadura ] was clarified in a polyphasic study. The organisms showed a combination of chemotaxonomic and morphological properties typical of Actinomadurae. They also formed distinct phyletic lines in the 16S rRNA Actinomadura gene tree; strain A288 T was associated with A. nitritigenes whereas strain A290 T was closely related to a group that consisted of A. citrea , A. coerulea , A. glauciflava , A. luteofluorescens and A. verrucosospora . Strains A288 T and A290 T showed key phenotypic features which readily distinguish them from one another and from representatives of related validly described species of Actinomadura . It is proposed that the two organisms be classified as new species of the genus Actinomadura . The names proposed for the new taxa are Actinomadura mexicana (A290 T = DSM 44485 T = NRRL B-24203 T ), and Actinomadura meyerii (A288 T = DSM 44485 T = NRRL B-24203 T ).
Tiago Domingues Zucchi - One of the best experts on this subject based on the ideXlab platform.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
Byung-yong Kim - One of the best experts on this subject based on the ideXlab platform.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
Avinash Naga Venkata Bonda - One of the best experts on this subject based on the ideXlab platform.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
-
Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Tiago Domingues Zucchi, Byung-yong Kim, Avinash Naga Venkata Bonda, Michael GoodfellowAbstract:The taxonomic position of a soil isolate, strain BK147T, was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Actinomadura . It formed a distinct phyletic line in the phylogenetic tree based on 16S rRNA gene sequences of members of the genus Actinomadura and was most closely, albeit loosely, related to Actinomadura bangladeshensis DSM 45347T, Actinomadura meyerae DSM 44715T and Actinomadura napierensis NRRL B-24319T but was readily distinguished from these strains using a range of phenotypic properties. Based on the combined genotypic and phenotypic data it is proposed that isolate BK147T ( = KACC 20919T = NCIMB 14771T = NRRL B-24852T) be classified as the type strain of a novel species of the genus Actinomadura , for which the name Actinomadura xylanilytica sp. nov. is proposed.
Takuji Kudo - One of the best experts on this subject based on the ideXlab platform.
-
Actinomadura decatromicini sp. nov., isolated from mountain soil in Thailand
The Journal of antibiotics, 2020Co-Authors: Apakorn Songsumanus, Takuji Kudo, Nattakorn Kuncharoen, Masahiro Yuki, Moriya Ohkuma, Yasuhiro Igarashi, Somboon TanasupawatAbstract:A novel actinomycete strain CYP1-5T was isolated from the mountain soil sample collected from Chaiyaphum province, Thailand and its taxonomic position was clarified by using a polyphasic taxonomic approach. The chemotaxonomic properties of strain CYP1-5T were consistent within the genus Actinomadura. Cell-wall peptidoglycan of this strain contained meso-diaminopimelic acid. Galactose, madurose, and ribose were presented as the diagnostic sugars in whole-cell hydrolysates. The major menaquinone was MK-9(H6). Major cellular fatty acids were iso-C16:0 and C16:0. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, and phosphatidylinositol mannoside were observed as predominant phospholipids. Based on the results of phylogenetic analyses of 16S rRNA gene sequence, strain CYP1-5T was constituent with the genus Actinomadura and was closely related to Actinomadura syzygii GKU157T (99.5%) and Actinomadura chibensis IFM 10266T (= JCM 14158T) (98.2%). The draft genome size of strain CYP1-5T was 9.30 Mb with 72.2 mol% of G + C content. Strain CYP1-5T showed ANIb values of 94.9% with A. syzygii GKU157T and 93.2% with A. chibensis JCM 14158T. Phenotypic characteristics, phylogenetic analysis and genome data support that strain CYP1-5T could be discriminated from its closest relatives, representing a novel species of the genus Actinomadura, for which the name Actinomadura decatromicini sp. nov. is proposed. The type strain is CYP1-5T (= JCM 16996T = KCTC 19916T = TISTR 2901T).
-
Actinomadura montaniterrae sp. nov., isolated from mountain soil.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Apakorn Songsumanus, Takuji Kudo, Wongsakorn Phongsopitanun, Moriya Ohkuma, Somboon TanasupawatAbstract:The taxonomic position of the mountain soil actinomycete, strain CYP1-1BT, was clarified by a polyphasic study. The strain produced a single spore, or occasionally a chain of spores, on aerial mycelium. Chemotaxonomic data supported the classification of CYP1-1BT as representing a member of the genus Actinomadura on the basis of the presence of meso-diaminopimelic acid in the peptidoglycan; galactose, glucose, madurose and ribose as whole cell sugars; MK-9(H6), MK-9(H8) and MK-9(H4) as dominant menaquinones; C16 : 0, 10-methylated C18 : 0 and C18 : 1ω9c as the major cellular fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol manosides as the predominant phospholipids. The DNA G+C content was 74.3 mol%. On the basis of the combination of morphological and chemotaxonomic characteristics, CYP1-1BT was identified as representing a member of the genus Actinomadura . On the basis of the results of 16S rRNA gene analysis, CYP1-1BT, was shown to be closely related to Actinomadura nitritigenes DSM 44137T (98.9 %). Phenotypic, genotypic and DNA–DNA hybridization data supported the hypothesis that CYP1-1BT represents a novel species of the genus Actinomadura for which the name Actinomadura montaniterrae sp. nov. is proposed. The type strain is CYP1-1BT (=JCM 16995T=KCTC 39784T=PCU 349T=TISTR 2400T).
-
Actinomadura rayongensis sp. nov., isolated from peat swamp forest soil.
International journal of systematic and evolutionary microbiology, 2014Co-Authors: Wongsakorn Phongsopitanun, Somboon Tanasupawat, Moriya Ohkuma, Khanit Suwanborirux, Takuji KudoAbstract:A novel actinomycete strain RY35-68(T), isolated from a peat swamp forest soil sample in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus Actinomadura based on morphological and chemotaxonomic characteristics. Cell-wall analysis revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan layer. The diagnostic sugar in whole-cell hydrolysates was identified as madurose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major cellular fatty acids were C16 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The genomic DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY35-68(T) was closely related to the species Actinomadura atramentaria JCM 6250(T) (97.5 %). The value of DNA-DNA relatedness between strain RY35-68(T) and A. atramentaria JCM 6250(T) was 37.6-42.6 %. On the basis of its phenotypic characteristics and these results mentioned, this strain could be distinguished from the closely related type strain and represents a novel species of the genus Actinomadura, for which the name Actinomadura rayongensis sp. nov. (type strain RY35-68(T) = JCM 19830(T) = TISTR 2211(T) = PCU 332(T)) is proposed.
-
Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al. 1987) sp. nov., comb. nov.
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Yaowanoot Promnuan, Takuji Kudo, Moriya Ohkuma, Panuwan ChantawannakulAbstract:A Gram-reaction-positive aerobic actinomycete, designated strain IM17-1T, was isolated from a honey bee (Apis mellifera) hive in Chiang Mai Province, Thailand. The strain formed a branched substrate mycelium and mature aerial mycelium bore short chains of arthrospores with warty surfaces. The cell wall contained meso-2,6-diaminopimelic acid (cell-wall type III) and the whole cell sugars were fucose, galactose, glucose, madurose, mannose and ribose. The major isoprenoid quinone was hexahydrogenated menaquinone with nine isoprene units and the predominant cellular fatty acids were C16 : 0 (33.8 %), C18 : 1ω9c (32.7 %), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) (8.7 %) and 10-methyl C18 : 0 (8.2 %). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. These morphological and chemotaxonomic characteristics were consistent with the classification of IM17-1T within the genus Actinomadura. Based on 16S rRNA gene sequence analysis, strain IM17-1T was closely related to the type strains of Actinomadura cremea subsp. cremea (98.1 %) and Actinomadura cremea subsp. rifamycini (98.6 %); however, it represented a distinct phylogenetic lineage from the other species within this genus. The unique genetic characteristics were reaffirmed by low levels of DNA–DNA relatedness between strain IM17-1T and the two most closely related type strains, A. cremea subsp. cremea JCM 3308T (56.5±4.9 %) and A. cremea subsp. rifamycini JCM 3309T (31.0±22.6 %), and further supported the proposal of IM17-1T as a novel species. Strain IM17-1T ( = JCM 16576T = TISTR 1980T) thus represents a novel species of the genus Actinomadura, for which the name Actinomadura apis sp. nov. is proposed. In addition, the genotypic and phenotypic data suggested the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as a separate species, Actinomadura rifamycini sp. nov., comb. nov.
-
Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008Co-Authors: Ismet Ara, Atsuko Matsumoto, M. A. Bakir, Takuji Kudo, Satoshi Omura, Yoko TakahashiAbstract:The taxonomic position of two soil isolates, 3-46-b(3)T and 3-45-a(11)T, was clarified based on data from a polyphasic study. The organisms showed a combination of chemotaxonomic and morphological properties typical of the genus Actinomadura. They formed distinct phyletic lines in the Actinomadura 16S rRNA gene tree and were closely associated with the type strains of Actinomadura meyerae (sequence similarity of 98.3–98.5 %), Actinomadura napierensis (98.1–98.3 %) and Actinomadura latina (96.4–96.8 %). The level of 16S rRNA gene sequence similarity between the new isolates was 99.1 %. The level of DNA–DNA hybridization between strains 3-46-b(3)T and 3-45-a(11)T was 43.6 % and levels of relatedness between the two new isolates and the type strains of A. meyerae and A. napierensis were 21.0–27.3 %. On the basis of phenotypic and genotypic properties, the new isolates could be differentiated from each other and from their closest phylogenetic relatives. It is proposed that the organisms be classified as representing two novel species of the genus Actinomadura. The names proposed for these taxa are Actinomadura bangladeshensis sp. nov. [type strain 3-46-b(3)T =JCM 13933T =MTCC8057T] and Actinomadura chokoriensis sp. nov. [type strain 3-45-a(11)T =JCM 13932T =MTCC8056T].