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Matthew D. Collins - One of the best experts on this subject based on the ideXlab platform.
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Actinomyces dentalis sp nov from a human dental abscess
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Val Hall, Matthew D. Collins, Enevold Falsen, Paul A Lawson, Brian I DuerdenAbstract:A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus Actinomyces, clustering within a group of species that includes Actinomyces Bovis, the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, Actinomyces dentalis sp. nov. The type strain is R18165T (=CCUG 48064T=CIP 108337T).
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Actinomyces oricola sp. nov., from a human dental abscess.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Val Hall, Matthew D. Collins, Roger A Hutson, Elisabeth Inganäs, Enevold Falsen, Brian I DuerdenAbstract:A previously undescribed Actinomyces-like bacterium was isolated from a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a hitherto unknown subline within the genus Actinomyces, clustering within a group of species, which includes Actinomyces Bovis, the type species of the genus. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unknown organism recovered from a dental abscess be classified as a new species, Actinomyces oricola sp. nov. The type strain of Actinomyces oricola is R5292(T) (=CCUG 46090(T)=CIP 107639(T)).
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characterization of Actinomyces isolates from infected root canals of teeth description of Actinomyces radicidentis sp nov
Journal of Clinical Microbiology, 2000Co-Authors: Matthew D. Collins, Lesley Hoyles, Natalia Nikolaitchouk, Sotos Kalfas, Goran Sundquist, Tor Monsen, Enevold FalsenAbstract:Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces Bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence, we propose that the unknown bacterium isolated from human clinical specimens be classified as Actinomyces radicidentis sp. nov. The type strain of Actinomyces radicidentis is CCUG 36733.
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characterization of Actinomyces isolates from samples from the human urogenital tract description of Actinomyces urogenitalis sp nov
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Natalia Nikolaitchouk, Lesley Hoyles, John M Grainger, Enevold Falsen, Matthew D. CollinsAbstract:Three strains of a previously undescribed Actinomyces-like bacterium were isolated from human clinical sources (urine, urethra and vaginal secretion). Biochemical testing and PAGE analysis of whole-cell proteins indicated that the strains were phenotypically homogeneous and distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces Bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium from humans be classified as Actinomyces urogenitalis sp. nov. The type strain of Actinomyces urogenitalis is CCUG 38702T (= CIP 106421T).
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Actinomyces graevenitzii sp nov isolated from human clinical specimens
International Journal of Systematic and Evolutionary Microbiology, 1997Co-Authors: Cristina Pascual Ramos, Berit Sjödén, Eva Akervall, Enevold Falsen, Nerea Alvarez, Matthew D. CollinsAbstract:Four strains of a previously unknown, catalase-negative, facultatively anaerobic, gram-positive, rod-shaped organism originating from humans were characterized by biochemical, chemical, and molecular taxonomic methods. The four strains phenotypically closely resembled one another, and although they possessed characteristics consistent with membership in the genus Actinomyces, they differed from all previously recognized species of this genus. The results of comparative 16S rRNA gene sequencing studies demonstrated that the unknown human bacterium was phylogenetically a member of the genus Actinomyces. Within the genus Actinomyces, the unidentified bacterium formed a loose, but statistically significant, association with a subgroup which included Actinomyces Bovis, the type species of the genus. 16S rRNA sequence divergence values of >6%, however, unequivocally demonstrated that the unidentified bacterium represents a new subline of the genus Actinomyces. A new species, Actinomyces graevenitzii, is proposed for the four new isolates. The type strain of A. graevenitzii is CCUG 27294.
Enevold Falsen - One of the best experts on this subject based on the ideXlab platform.
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Actinomyces dentalis sp nov from a human dental abscess
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Val Hall, Matthew D. Collins, Enevold Falsen, Paul A Lawson, Brian I DuerdenAbstract:A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus Actinomyces, clustering within a group of species that includes Actinomyces Bovis, the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, Actinomyces dentalis sp. nov. The type strain is R18165T (=CCUG 48064T=CIP 108337T).
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Actinomyces oricola sp. nov., from a human dental abscess.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Val Hall, Matthew D. Collins, Roger A Hutson, Elisabeth Inganäs, Enevold Falsen, Brian I DuerdenAbstract:A previously undescribed Actinomyces-like bacterium was isolated from a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a hitherto unknown subline within the genus Actinomyces, clustering within a group of species, which includes Actinomyces Bovis, the type species of the genus. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unknown organism recovered from a dental abscess be classified as a new species, Actinomyces oricola sp. nov. The type strain of Actinomyces oricola is R5292(T) (=CCUG 46090(T)=CIP 107639(T)).
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characterization of Actinomyces isolates from infected root canals of teeth description of Actinomyces radicidentis sp nov
Journal of Clinical Microbiology, 2000Co-Authors: Matthew D. Collins, Lesley Hoyles, Natalia Nikolaitchouk, Sotos Kalfas, Goran Sundquist, Tor Monsen, Enevold FalsenAbstract:Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces Bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence, we propose that the unknown bacterium isolated from human clinical specimens be classified as Actinomyces radicidentis sp. nov. The type strain of Actinomyces radicidentis is CCUG 36733.
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characterization of Actinomyces isolates from samples from the human urogenital tract description of Actinomyces urogenitalis sp nov
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Natalia Nikolaitchouk, Lesley Hoyles, John M Grainger, Enevold Falsen, Matthew D. CollinsAbstract:Three strains of a previously undescribed Actinomyces-like bacterium were isolated from human clinical sources (urine, urethra and vaginal secretion). Biochemical testing and PAGE analysis of whole-cell proteins indicated that the strains were phenotypically homogeneous and distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces Bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium from humans be classified as Actinomyces urogenitalis sp. nov. The type strain of Actinomyces urogenitalis is CCUG 38702T (= CIP 106421T).
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Actinomyces graevenitzii sp nov isolated from human clinical specimens
International Journal of Systematic and Evolutionary Microbiology, 1997Co-Authors: Cristina Pascual Ramos, Berit Sjödén, Eva Akervall, Enevold Falsen, Nerea Alvarez, Matthew D. CollinsAbstract:Four strains of a previously unknown, catalase-negative, facultatively anaerobic, gram-positive, rod-shaped organism originating from humans were characterized by biochemical, chemical, and molecular taxonomic methods. The four strains phenotypically closely resembled one another, and although they possessed characteristics consistent with membership in the genus Actinomyces, they differed from all previously recognized species of this genus. The results of comparative 16S rRNA gene sequencing studies demonstrated that the unknown human bacterium was phylogenetically a member of the genus Actinomyces. Within the genus Actinomyces, the unidentified bacterium formed a loose, but statistically significant, association with a subgroup which included Actinomyces Bovis, the type species of the genus. 16S rRNA sequence divergence values of >6%, however, unequivocally demonstrated that the unidentified bacterium represents a new subline of the genus Actinomyces. A new species, Actinomyces graevenitzii, is proposed for the four new isolates. The type strain of A. graevenitzii is CCUG 27294.
Brian I Duerden - One of the best experts on this subject based on the ideXlab platform.
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Actinomyces dentalis sp nov from a human dental abscess
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Val Hall, Matthew D. Collins, Enevold Falsen, Paul A Lawson, Brian I DuerdenAbstract:A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus Actinomyces, clustering within a group of species that includes Actinomyces Bovis, the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, Actinomyces dentalis sp. nov. The type strain is R18165T (=CCUG 48064T=CIP 108337T).
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Actinomyces oricola sp. nov., from a human dental abscess.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Val Hall, Matthew D. Collins, Roger A Hutson, Elisabeth Inganäs, Enevold Falsen, Brian I DuerdenAbstract:A previously undescribed Actinomyces-like bacterium was isolated from a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a hitherto unknown subline within the genus Actinomyces, clustering within a group of species, which includes Actinomyces Bovis, the type species of the genus. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unknown organism recovered from a dental abscess be classified as a new species, Actinomyces oricola sp. nov. The type strain of Actinomyces oricola is R5292(T) (=CCUG 46090(T)=CIP 107639(T)).
Val Hall - One of the best experts on this subject based on the ideXlab platform.
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Actinomyces dentalis sp nov from a human dental abscess
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Val Hall, Matthew D. Collins, Enevold Falsen, Paul A Lawson, Brian I DuerdenAbstract:A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus Actinomyces, clustering within a group of species that includes Actinomyces Bovis, the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, Actinomyces dentalis sp. nov. The type strain is R18165T (=CCUG 48064T=CIP 108337T).
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Actinomyces oricola sp. nov., from a human dental abscess.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Val Hall, Matthew D. Collins, Roger A Hutson, Elisabeth Inganäs, Enevold Falsen, Brian I DuerdenAbstract:A previously undescribed Actinomyces-like bacterium was isolated from a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a hitherto unknown subline within the genus Actinomyces, clustering within a group of species, which includes Actinomyces Bovis, the type species of the genus. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unknown organism recovered from a dental abscess be classified as a new species, Actinomyces oricola sp. nov. The type strain of Actinomyces oricola is R5292(T) (=CCUG 46090(T)=CIP 107639(T)).
Lydston Rodrigues De ,carvalho - One of the best experts on this subject based on the ideXlab platform.
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Microbiota bacteriana dominante cultivável no trato digestivo do urubu (Coragyps atratus Bechstein 1793)
Sociedade Brasileira de Microbiologia, 2003Co-Authors: Lydston Rodrigues De ,carvalho, Farias, Luiz Macêdo, Nicoli, Jacques Robert, Silva, Maria Clara Fernandes, Corsino, Andrea Teresa Santos Meira, Luiz André De ,lima, Redondo, Rodrigo Aparecido Fernandes, Ferreira, Paulo César Peregrino, Pinto, Maria Elizabeth Bernardes MarguttiAbstract:Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them. The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37 log CFU/g in the distal intestine. Some bacteria were isolated for the first time from the digestive tract of C. atratus: Actinomyces Bovis, Lactobacillus cellobiosus, Micrococcus luteus, Neisseria sicca, Clostridium bifermentans, Enterobacter agglomerans, Peptostreptococcus sp., Sarcina sp., Serratia odorifera, and Shigella flexneri. Associations between microorganisms were observed during isolation on two occasions, one involving A. Bovis and N. sicca, and the other involving A. Bovis and a Gram-negative rod. Hetero-, iso- and autoantagonisms were observed, suggesting the ecological role of these indigenous microorganisms in terms of population auto-control and environmental barrier in the digestive tract of carrion-feeding birds.As bactérias anaeróbias estritas e facultativas cultiváveis do trato digestivo de seis urubus (Coragyps atratus Bechstein 1793) foram isoladas e identificadas. Após a captura, as aves receberam uma alimentação de baixa contaminação durante uma semana para eliminar possíveis microorganismos alóctonos. A seguir, amostras colhidas na língua, estomago e intestinos foram pesadas, submetidas a diluições decimais numa câmara anaeróbia, inoculadas em meios de cultura e incubadas em aerobiose e anaerobiose a 37ºC para enumeração, isolamento e identificação. As bactérias isoladas foram usadas posteriormente como produtoras e reveladoras para detectar possíveis fenômenos de antagonismo. A população bacteriana total ao longo do trato digestivo variou de 3,46 ± 0,39 log UFC/g no estômago até 10,75 ± 0,37 log UFC/g no intestino distal. Algumas bactérias foram isoladas pela primeira vez do trato digestivo de C. atratus: Actinomyces Bovis, Lactobacillus cellobiosus, Micrococcus luteus, Neisseria sicca, Clostridium bifermentans, Enterobacter agglomerans, Peptostreptococcus sp., Sarcina sp., Serratia odorifera, and Shigella flexneri. Associações entre microorganismos foram observadas durante o isolamento em dois casos, um envolvendo A. Bovis e N. sicca, e o outro envolvendo A. Bovis e um bastonete Gram-negativo. Hetero-, iso- e autoantagonismos foram observados, sugerindo um papel ecológico para esses microorganismos em termos de autocontrole populacional e de barreira ambiental no trato digestivo dessas aves
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Dominant culturable bacterial microbiota in the digestive tract of the American black vulture (Coragyps atratus Bechstein 1793) and search for antagonistic substances
Sociedade Brasileira de Microbiologia, 2003Co-Authors: Lydston Rodrigues De ,carvalho, Farias, Luiz Macêdo, Nicoli, Jacques Robert, Silva, Maria Clara Fernandes, Corsino, Andrea Teresa Santos Meira, Luiz André De ,lima, Redondo, Rodrigo Aparecido Fernandes, Ferreira, Paulo César Peregrino, Pinto, Maria Elizabeth Bernardes MarguttiAbstract:Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them. The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37 log CFU/g in the distal intestine. Some bacteria were isolated for the first time from the digestive tract of C. atratus: Actinomyces Bovis, Lactobacillus cellobiosus, Micrococcus luteus, Neisseria sicca, Clostridium bifermentans, Enterobacter agglomerans, Peptostreptococcus sp., Sarcina sp., Serratia odorifera, and Shigella flexneri. Associations between microorganisms were observed during isolation on two occasions, one involving A. Bovis and N. sicca, and the other involving A. Bovis and a Gram-negative rod. Hetero-, iso- and autoantagonisms were observed, suggesting the ecological role of these indigenous microorganisms in terms of population auto-control and environmental barrier in the digestive tract of carrion-feeding birds
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Dominant culturable bacterial microbiota in the digestive tract of the American black vulture (Coragyps atratus Bechstein 1793) and search for antagonistic substances Microbiota bacteriana dominante cultivável no trato digestivo do urubu (Coragyps atratus Bechstein 1793)
Sociedade Brasileira de Microbiologia, 2003Co-Authors: Lydston Rodrigues De ,carvalho, Luiz André De ,lima, Luiz Macêdo Farias, Jacques Robert Nicoli, Maria Clara Fernandes Silva, Andrea Teresa Santos Meira Corsino, Rodrigo Aparecido Fernandes Redondo, Paulo César Peregrino Ferreira, Maria Elizabeth Bernardes Margutti PintoAbstract:Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them. The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37 log CFU/g in the distal intestine. Some bacteria were isolated for the first time from the digestive tract of C. atratus: Actinomyces Bovis, Lactobacillus cellobiosus, Micrococcus luteus, Neisseria sicca, Clostridium bifermentans, Enterobacter agglomerans, Peptostreptococcus sp., Sarcina sp., Serratia odorifera, and Shigella flexneri. Associations between microorganisms were observed during isolation on two occasions, one involving A. Bovis and N. sicca, and the other involving A. Bovis and a Gram-negative rod. Hetero-, iso- and autoantagonisms were observed, suggesting the ecological role of these indigenous microorganisms in terms of population auto-control and environmental barrier in the digestive tract of carrion-feeding birds.As bactérias anaeróbias estritas e facultativas cultiváveis do trato digestivo de seis urubus (Coragyps atratus Bechstein 1793) foram isoladas e identificadas. Após a captura, as aves receberam uma alimentação de baixa contaminação durante uma semana para eliminar possíveis microorganismos alóctonos. A seguir, amostras colhidas na língua, estomago e intestinos foram pesadas, submetidas a diluições decimais numa câmara anaeróbia, inoculadas em meios de cultura e incubadas em aerobiose e anaerobiose a 37ºC para enumeração, isolamento e identificação. As bactérias isoladas foram usadas posteriormente como produtoras e reveladoras para detectar possíveis fenômenos de antagonismo. A população bacteriana total ao longo do trato digestivo variou de 3,46 ± 0,39 log UFC/g no estômago até 10,75 ± 0,37 log UFC/g no intestino distal. Algumas bactérias foram isoladas pela primeira vez do trato digestivo de C. atratus: Actinomyces Bovis, Lactobacillus cellobiosus, Micrococcus luteus, Neisseria sicca, Clostridium bifermentans, Enterobacter agglomerans, Peptostreptococcus sp., Sarcina sp., Serratia odorifera, and Shigella flexneri. Associações entre microorganismos foram observadas durante o isolamento em dois casos, um envolvendo A. Bovis e N. sicca, e o outro envolvendo A. Bovis e um bastonete Gram-negativo. Hetero-, iso- e autoantagonismos foram observados, sugerindo um papel ecológico para esses microorganismos em termos de autocontrole populacional e de barreira ambiental no trato digestivo dessas aves
