The Experts below are selected from a list of 530787 Experts worldwide ranked by ideXlab platform
Peter W Zandstra - One of the best experts on this subject based on the ideXlab platform.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
Joseph Itskovitzeldor - One of the best experts on this subject based on the ideXlab platform.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
Sharon Gerechtnir - One of the best experts on this subject based on the ideXlab platform.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
Stephen Dang - One of the best experts on this subject based on the ideXlab platform.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
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controlled scalable embryonic stem Cell differentiation culture
Stem Cells, 2004Co-Authors: Stephen Dang, Sharon Gerechtnir, Jinny Chen, Joseph Itskovitzeldor, Peter W ZandstraAbstract:Embryonic stem (ES) Cells are of significant interest as a renewable source of therapeutically useful Cells. ES Cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES Cell derivatives. Aggregation between EBs (agglomeration), however, inhibits Cell growth and differentiation in stirred or high-Cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated Cell attachment and followed by Active Cell migration. We report the development of a technology capable of controlling Cell-Cell interactions in scalable culture by the mass encapsulation of ES Cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES Cells can be used to produce scalable quantities of hematopoietic progenitor Cells in a controlled environment.
Wilfried Bursch - One of the best experts on this subject based on the ideXlab platform.
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Active Cell death induced by the anti estrogens tamoxifen and ici 164 384 in human mammary carcinoma Cells mcf 7 in culture the role of autophagy
Carcinogenesis, 1996Co-Authors: Wilfried Bursch, Adolf Ellinger, Harald Kienzl, Ladislaus Torok, Siyaram Pandey, Marianna Sikorska, Roy Walker, Rolf Schulte HermannAbstract:Active Cell death in hormone-dependent Cells was studied using cultured human mammary carcinoma Cells (MCF-7) treated with the anti-estrogens (AEs) tamoxifen (TAM), 4-hydroxy-tamoxifen (OH-TAM) or ICI 164 384 (10 -8 -10 -5 M) as a model. The following results were obtained. (i) In untreated MCF-7 Cells a wave of replication occurred in the first 5 days of culture. All three AEs caused a dose-dependent inhibition of Cell replication. (ii) TAM and OH-TAM at 10 -5 M, but not ICI 164 384, caused lytic Cell death (necrosis) within 24 h, which was not inhibited by estradiol (10 -9 -10 -6 M). (iii) Lower concentrations of TAM or OH-TAM (up to 10 -6 M) or ICI 164 384 induced a more gradual appearance of Cell death beginning at day 3. This type of Cell death was inhibited by estradiol (10 -9 M), indicating its Active nature. (iv) Nuclei showed two distinct patterns of alteration : (a) apoptosis-like condensation and fragmentation of chromatin to crescent masses abutting the nuclear envelope ; (b) condensation of the chromatin to a single, pyknotic mass in the center of the nucleus, detached from the nuclear envelope. Quantitative histological evaluation revealed the predominance of pyknosis. (v) Biochemical DNA analysis revealed that only a relatively small amount of the total DNA was finally degraded into low molecular weight fragments (20 kb and less). (vi) Active Cell death, with both apoptotic and pyknotic nuclear morphology, was associated with extensive formation of autophagic vacuoles (AV). 3-Methyladenine, a known inhibitor of AV formation, partially prevented Cell death as detected by nuclear changes. (vii) ICI 164 384 was about 10 times more effective than TAM or OH-TAM at inhibiting DNA synthesis, but had equal potency in inducing Active Cell death. It is concluded that AEs have anti-proliferative and anti-survival effects on MCF-7 human mammary cancer Cells in culture. These two effects are under separate control because they differ by kinetics, dose dependence and sensitivity to the various AEs. Active Cell death in MCF-7 Cells seems to be initiated by autophagy, in contrast to concepts of apoptosis, and thus corresponds to autophagic/lysosomal or type II death as previously defined. This may be important because of biochemical and molecular differences between these various subtypes of Active Cell death.
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rapid communicationin situ detection of fragmented dna tunel assay fails to discriminate among apoptosis necrosis and autolytic Cell death a cautionary note
Hepatology, 1995Co-Authors: Bettina Graslkraupp, Helga Koudelka, Krystyna Bukowska, Wilfried Bursch, B Ruttkaynedecky, Rolf SchultehermannAbstract:Detection of DNA fragments in situ using the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate Active Cell death (apoptosis). We studied the specificity of the assay in well-defined models of apoptosis and necrosis as well as in postmortem autolysis in rat liver. During involution of liver hyperplasia, which follows stopping treatment with the hepatomitogens cyproterone acetate (CPA) or nafenopin (NAF), numerous apoptotic hepatocytes could be observed with TUNEL-positive chromatin residues. A similar TUNEL-positive reaction appeared in necrotic hepatocytes after a cytotoxic dose of carbon tetrachloride (CC14) or N-nitrosomorpholine (NNM). Also, in insufficiently fixed, autolytic livers TUNEL-positive nuclei were observed. Thus, DNA fragmentation is common to different kinds of Cell death; its detection in situ should not be considered a specific marker of apoptosis.
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In situ detection of fragmented DNA (tunel assay) fails to discriminate among apoptosis, necrosis, and autolytic Cell death: A cautionary note
Hepatology, 1995Co-Authors: Bettina Grasl-kraupp, Helga Koudelka, Krystyna Bukowska, Wilfried Bursch, Branislav Ruttkay-nedecky, Rolf Schulte-hermannAbstract:Detection of DNA fragments in situ using the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate Active Cell death (apoptosis). We studied the specificity of the assay in well-defined models of apoptosis and necrosis as well as in postmortem autolysis in rat liver. During involution of liver hyperplasia, which follows stopping treatment with the hepatomitogens cyproterone acetate (CPA) or nafenopin (NAF), numerous apoptotic hepatocytes could be observed with TUNEL-positive chromatin residues. A similar TUNEL-positive reaction appeared in necrotic hepatocytes after a cytotoxic dose of carbon tetrachloride (CC14) or N-nitrosomorpholine (NNM). Also, in insufficiently fixed, autolytic livers TUNEL-positive nuclei were observed. Thus, DNA fragmentation is common to different kinds of Cell death; its detection in situ should not be considered a specific marker of apoptosis. © 1995.
