The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Julian W. Tang - One of the best experts on this subject based on the ideXlab platform.
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an Adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis a possible failure of contact and aerosol infection control
American Journal of Infection Control, 2016Co-Authors: Elizabeth Hoyle, Joanne C. Erez, Elizabeth Collins, Helen R Kirkgranger, Julian W. TangAbstract:An Adenovirus serotype 4 outbreak was identified on a pediatric ward involving 4 members of the health care staff. Two inpatients on the ward at the time (1 immunocompromised) were shedding this virus from their respiratory tracts and could have acted as independent index cases for the staff infections. Significantly, upon investigation, it was found that staff members were unaware that Adenoviruses are not completely eliminated by alcohol gel handrubs and that soap and water handwashing is also required.
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An Adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis—a possible failure of contact and aerosol infection control
American journal of infection control, 2016Co-Authors: Elizabeth Hoyle, Joanne C. Erez, Helen R. Kirk-granger, Elizabeth Collins, Julian W. TangAbstract:An Adenovirus serotype 4 outbreak was identified on a pediatric ward involving 4 members of the health care staff. Two inpatients on the ward at the time (1 immunocompromised) were shedding this virus from their respiratory tracts and could have acted as independent index cases for the staff infections. Significantly, upon investigation, it was found that staff members were unaware that Adenoviruses are not completely eliminated by alcohol gel handrubs and that soap and water handwashing is also required.
Elizabeth Hoyle - One of the best experts on this subject based on the ideXlab platform.
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an Adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis a possible failure of contact and aerosol infection control
American Journal of Infection Control, 2016Co-Authors: Elizabeth Hoyle, Joanne C. Erez, Elizabeth Collins, Helen R Kirkgranger, Julian W. TangAbstract:An Adenovirus serotype 4 outbreak was identified on a pediatric ward involving 4 members of the health care staff. Two inpatients on the ward at the time (1 immunocompromised) were shedding this virus from their respiratory tracts and could have acted as independent index cases for the staff infections. Significantly, upon investigation, it was found that staff members were unaware that Adenoviruses are not completely eliminated by alcohol gel handrubs and that soap and water handwashing is also required.
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An Adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis—a possible failure of contact and aerosol infection control
American journal of infection control, 2016Co-Authors: Elizabeth Hoyle, Joanne C. Erez, Helen R. Kirk-granger, Elizabeth Collins, Julian W. TangAbstract:An Adenovirus serotype 4 outbreak was identified on a pediatric ward involving 4 members of the health care staff. Two inpatients on the ward at the time (1 immunocompromised) were shedding this virus from their respiratory tracts and could have acted as independent index cases for the staff infections. Significantly, upon investigation, it was found that staff members were unaware that Adenoviruses are not completely eliminated by alcohol gel handrubs and that soap and water handwashing is also required.
Balazs Harrach - One of the best experts on this subject based on the ideXlab platform.
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Crystal structure of the fibre head domain of bovine Adenovirus 4, a ruminant atAdenovirus
Virology Journal, 2015Co-Authors: Thanh H. Nguyen, Balazs Harrach, Mónika Z. Ballmann, Maria Benkő, Márton Z. Vidovszky, Marta Sanz-gaitero, Abhimanyu K. Singh, Monika Z Ballmann, Mark J. Van RaaijAbstract:Background In Adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other Adenovirus capsid proteins and leads to a productive infection. Although many fibre head structures are known for human mastAdenoviruses, not many animal Adenovirus fibre head structures have been determined, especially not from those belonging to Adenovirus genera other than MastAdenovirus . Methods We constructed an expression vector for the fibre head domain from a ruminant atAdenovirus, bovine Adenovirus 4 (BAdV-4), consisting of amino acids 414–535, expressed the protein in Escherichia coli , purified it by metal affinity and cation exchange chromatography and crystallized it. The structure was solved using single isomorphous replacement plus anomalous dispersion of a mercury derivative and refined against native data that extended to 1.2 Å resolution. Results Like in other Adenoviruses, the BAdV-4 fibre head monomer contains a beta-sandwich consisting of ABCJ and GHID sheets. The topology is identical to the fibre head of the other studied atAdenovirus, snake Adenovirus 1 (SnAdV-1), including the alpha-helix in the DG-loop, despite of them having a sequence identity of only 15 %. There are also differences which may have implications for ligand binding. Beta-strands G and H are longer and differences in several surface-loops and surface charge are observed. Conclusions Chimeric Adenovirus fibres have been used to retarget Adenovirus-based anti-cancer and gene therapy vectors. Ovine Adenovirus 7 (OAdV-7), another ruminant atAdenovirus, is intensively tested as a basis for such a vector. Here, we present the high-resolution atomic structure of the BAdV-4 fibre head domain, the second atAdenovirus fibre head structure known and the first of an atAdenovirus that infects a mammalian host. Future research should focus on the receptor-binding properties of these fibre head domains.
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Crystal structure of the fibre head domain of bovine Adenovirus 4, a ruminant atAdenovirus.
Virology Journal, 2015Co-Authors: Thanh H. Nguyen, Balazs Harrach, Mónika Z. Ballmann, Maria Benkő, Márton Z. Vidovszky, Marta Sanz-gaitero, Abhimanyu K. Singh, Monika Z Ballmann, Mark J. Van RaaijAbstract:Background In Adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other Adenovirus capsid proteins and leads to a productive infection. Although many fibre head structures are known for human mastAdenoviruses, not many animal Adenovirus fibre head structures have been determined, especially not from those belonging to Adenovirus genera other than MastAdenovirus.
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complete genome sequences of pigeon Adenovirus 1 and duck Adenovirus 2 extend the number of species within the genus aviAdenovirus
Virology, 2014Co-Authors: Ana Marek, Balazs Harrach, Győző L Kajan, Carolin Kosiol, Christian Schlotterer, Michael HessAbstract:Abstract Complete genomes of the first isolates of pigeon Adenovirus 1 (PiAdV-1) and Muscovy duck Adenovirus (duck Adenovirus 2, DAdV-2) were sequenced. The PiAdV-1 genome is 45,480 bp long, and has a gene organization most similar to turkey Adenovirus 1. Near the left end of the genome, it lacks ORF0, ORF1A, ORF1B and ORF1C, and possesses ORF52, whereas six novel genes were found near the right end. The DAdV-2 genome is 43,734 bp long, and has a gene organization similar to that of goose Adenovirus 4 (GoAdV-4). It lacks ORF51, ORF1C and ORF54, and possesses ORF55A and five other novel genes. PiAdV-1 and DAdV-2 genomes contain two and one fiber genes, respectively. Genome organization, G+C content, molecular phylogeny and host type confirm the need to establish two novel species (Pigeon aviAdenovirus A and Duck aviAdenovirus B) within the genus AviAdenovirus. Phylogenetic data show that DAdV-2 is most closely related to GoAdV-4.
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whole genome sequences of two turkey Adenovirus types reveal the existence of two unknown lineages that merit the establishment of novel species within the genus aviAdenovirus
Journal of General Virology, 2014Co-Authors: Ana Marek, Balazs Harrach, Monika Z Ballmann, Carolin Kosiol, Christian Schlotterer, Michael HessAbstract:There are eight species established for aviAdenoviruses: Fowl Adenovirus A–E, Goose Adenovirus A, Falcon Adenovirus A and Turkey Adenovirus B. The aim of this study was to sequence and analyse the complete genomes of turkey Adenovirus 4 (TAdV-4) and TAdV-5 (strain 1277BT) in addition to almost two-thirds of the genome of another TAdV-5 strain (strain D1648). By applying next-generation sequencing, the full genomes were found to be 42 940 and 43 686 bp and the G+C content was 48.5 and 51.6 mol% for TAdV-4 and TAdV-5, respectively. One fiber gene was identified in TAdV-4, whereas two fiber genes were found in TAdV-5. The genome organization of TAdV-4 resembled that of fowl Adenovirus 5 (FAdV-5), but it had ORF1C near the left end of the genome. TAdV-4 also had five 123 bp tandem repeats followed by five 33 bp tandem repeats, but they occurred before and not after ORF8, as in several fowl Adenoviruses. The genome organization of TAdV-5 was almost the same as that of FAdV-1 but with a possible difference in the splicing pattern of ORF11 and ORF26. Phylogenetic analyses and G+C content showed differences that seem to merit the establishment of two new species within the genus AviAdenovirus: Turkey Adenovirus C (for TAdV-4) and Turkey Adenovirus D (for TAdV-5). Our analyses suggest a common evolutionary origin of TAdV-5 and FAdV-1.
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genome sequence of a waterfowl aviAdenovirus goose Adenovirus 4
Journal of General Virology, 2012Co-Authors: Győző L Kajan, Balazs Harrach, Andrew J. Davison, Vilmos Palya, Maria BenkőAbstract:We present, to our knowledge, the first complete genome sequence of a waterfowl aviAdenovirus, goose Adenovirus (GoAdV) strain P29, and an analysis of its genetic content in comparison with five published aviAdenovirus genome sequences. Of the 35 genes predicted to encode functional proteins, the central region of the genome contains 19 (IVa2 to fiber-2) that were inherited from the ancestor of all known Adenoviruses. Of the remaining genes, nine have orthologues only in aviAdenoviruses and seven lack orthologues in any Adenovirus. We also obtained limited sequence data for a pathogenic GoAdV strain D1036/08. Phylogenetic analyses placed the two GoAdV strains monophyletically in the genus AviAdenovirus. We propose designating strains P29 and D1036/08 as GoAdV-4 and GoAdV-5, respectively.
Éva Nagy - One of the best experts on this subject based on the ideXlab platform.
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Fowl Adenovirus 4 (FAdV-4)-Based Infectious Clone for Vaccine Vector Development and Viral Gene Function Studies
MDPI AG, 2018Co-Authors: Yanlong Pei, Bryan D. Griffin, Juan C. Corredor, Peter J. Krell, Éva NagyAbstract:Fowl Adenovirus 4 (FAdV-4) is associated with economically important poultry diseases. Recent studies of fully sequenced genomes of FAdV-4 isolates suggest potential genomic regions associated with virulence and amenable for manipulation and vector development. Direct manipulation of viral genomes is cumbersome, as opposed to that of infectious clones—viral genomes cloned into plasmid or cosmid vectors. In this work, we generated an infectious clone, pFAdV-4 ON1, containing the entire viral genome of a nonpathogenic FAdV-4 (ON1 isolate). pFAdV-4 ON1 was used for targeted deletion of open reading frames (ORFs) 16 and 17 and replacement with the enhanced green fluorescence protein (EGFP) expression cassette to generate recombinant viruses. These viruses were viable, and EGFP was expressed in infected cells. Their replication, however, was significantly reduced with respect to that of the wild-type virus. These observations suggest the potential utility of FAdV-4 as a vaccine vector and the importance of ORFs 16 and 17 for virus replication at wild-type levels. To our knowledge, this is the first report of an infectious clone based on the FAdV-4 genome, and our results demonstrate its utility for studies of virulence determinants and as a platform for either vaccine or gene delivery vectors
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Coding potential and transcript analysis of fowl Adenovirus 4: insight into upstream ORFs as common sequence features in adenoviral transcripts.
Journal of General Virology, 2011Co-Authors: Bryan D. Griffin, Éva NagyAbstract:Recombinant fowl Adenoviruses (FAdVs) have been successfully used as veterinary vaccine vectors. However, insufficient definitions of the protein-coding and non-coding regions and an incomplete understanding of virus–host interactions limit the progress of next-generation vectors. FAdVs are known to cause several diseases of poultry. Certain isolates of species FAdV-C are the aetiological agent of inclusion body hepatitis/hydropericardium syndrome (IBH/HPS). In this study, we report the complete 45667 bp genome sequence of FAdV-4 of species FAdV-C. Assessment of the protein-coding potential of FAdV-4 was carried out with the Bio-Dictionary-based Gene Finder together with an evaluation of sequence conservation among species FAdV-A and FAdV-D. On this basis, 46 potentially protein-coding ORFs were identified. Of these, 33 and 13 ORFs were assigned high and low protein-coding potential, respectively. Homologues of the ancestral adenoviral genes were, with few exceptions, assigned high protein-coding potential. ORFs that were unique to the FAdVs were differentiated into high and low protein-coding potential groups. Notable putative genes with high protein-coding capacity included the previously unreported fiber 1, hypothetical 10.3K and hypothetical 10.5K genes. Transcript analysis revealed that several of the small ORFs less than 300 nt in length that were assigned low coding potential contributed to upstream ORFs (uORFs) in important mRNAs, including the ORF22 mRNA. Subsequent analysis of the previously reported transcripts of FAdV-1, FAdV-9, human Adenovirus 2 and bovine Adenovirus 3 identified widespread uORFs in AdV mRNAs that have the potential to act as important translational regulatory elements.
Joanne C. Erez - One of the best experts on this subject based on the ideXlab platform.
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an Adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis a possible failure of contact and aerosol infection control
American Journal of Infection Control, 2016Co-Authors: Elizabeth Hoyle, Joanne C. Erez, Elizabeth Collins, Helen R Kirkgranger, Julian W. TangAbstract:An Adenovirus serotype 4 outbreak was identified on a pediatric ward involving 4 members of the health care staff. Two inpatients on the ward at the time (1 immunocompromised) were shedding this virus from their respiratory tracts and could have acted as independent index cases for the staff infections. Significantly, upon investigation, it was found that staff members were unaware that Adenoviruses are not completely eliminated by alcohol gel handrubs and that soap and water handwashing is also required.
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An Adenovirus 4 outbreak amongst staff in a pediatric ward manifesting as keratoconjunctivitis—a possible failure of contact and aerosol infection control
American journal of infection control, 2016Co-Authors: Elizabeth Hoyle, Joanne C. Erez, Helen R. Kirk-granger, Elizabeth Collins, Julian W. TangAbstract:An Adenovirus serotype 4 outbreak was identified on a pediatric ward involving 4 members of the health care staff. Two inpatients on the ward at the time (1 immunocompromised) were shedding this virus from their respiratory tracts and could have acted as independent index cases for the staff infections. Significantly, upon investigation, it was found that staff members were unaware that Adenoviruses are not completely eliminated by alcohol gel handrubs and that soap and water handwashing is also required.