The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Mazen J. Hamadeh - One of the best experts on this subject based on the ideXlab platform.
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Vitamin D3 supplementation at 50x the Adequate Intake attenuates disease pathophysiology in the spinal cord of male, but is toxic in female, G93A mouse model of amyotrophic lateral sclerosis (ALS)
The FASEB Journal, 2015Co-Authors: Elnaz Moghimi, Alexandro Gianforcaro, Jesse Adam Solomon, Sanjeef Thampinathan, Mazen J. HamadehAbstract:Background: Vitamin D3 (D3) at 50x the Adequate Intake (AI) improves paw grip endurance in G93A mice. However, quadriceps apoptosis increases in females, indicating a threshold of toxicity. ALS is ...
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dietary vitamin d3 supplementation at 50 the Adequate Intake decreases calbindin d28k and endoplasmic reticulum stress and increases apoptosis suggesting toxicity in the female transgenic g93a mouse model of amyotrophic lateral sclerosis
The FASEB Journal, 2013Co-Authors: Siavash Taherishalmani, Alexandro Gianforcaro, Jesse Adam Solomon, Shayan Shahsavar, Mazen J. HamadehAbstract:BackgroundWe previously showed that dietary vitamin D3 (D3) at 50× the Adequate Intake increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), with ...
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vitamin d3 at 50x ai attenuates the decline in paw grip endurance but not disease outcomes in the g93a mouse model of als and is toxic in females
PLOS ONE, 2013Co-Authors: Alexandro Gianforcaro, Jesse Adam Solomon, Mazen J. HamadehAbstract:Background We previously demonstrated that dietary vitamin D3 at 10x the Adequate Intake (AI) attenuates the decline in functional capacity in the G93A mouse model of ALS. We hypothesized that higher doses would elicit more robust changes in functional and disease outcomes. Objective To determine the effects of dietary vitamin D3 at 50xAI on functional outcomes (motor performance, paw grip endurance) and disease severity (clinical score), as well as disease onset, disease progression and lifespan in the transgenic G93A mouse model of ALS. Methods Starting at age 25 d, 100 G93A mice (55 M, 45 F) were provided ad libitum with either an Adequate (AI; 1 IU D3/g feed) or high (HiD; 50 IU D3/g feed) vitamin D3 diet. Results HiD females consumed 9% less food corrected for body weight vs. AI females (P = 0.010). HiD mice had a 12% greater paw grip endurance over time between age 60–141 d (P = 0.015), and a 37% greater score during disease progression (P = 0.042) vs. AI mice. Although HiD females had a non-significant 31% greater CS prior to disease onset vs. AI females, they exhibited a significant 20% greater paw grip endurance AUC (P = 0.020) when corrected for clinical score. Conclusion Dietary D3 supplementation at 50x the Adequate Intake attenuated the decline in paw grip endurance, but did not influence age at disease onset, hindlimb paralysis or endpoint in the transgenic G93A mouse model of ALS. Furthermore, females may have reached the threshold for vitamin D3 toxicity as evidence by reduced food Intake and greater disease severity prior to disease onset.
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dietary vitamin d3 supplementation at 10 the Adequate Intake improves functional capacity in the g93a transgenic mouse model of als a pilot study
CNS Neuroscience & Therapeutics, 2012Co-Authors: Alexandro Gianforcaro, Mazen J. HamadehAbstract:SUMMARY Background: Vitamin D has antioxidant, anti-inflammatory, and neuroprotective properties, and may mitigate amyotrophic lateral sclerosis (ALS) pathology. Aims: To determine the effects of dietary vitamin D3 (D3) at 10-fold the Adequate Intake (AI) on functional and disease outcomes and lifespan in the transgenic G93A mouse model of ALS. Methods: Starting at age 40 days, 32 G93A mice (21 M, 11 F) were provided ad libitum with either an Adequate (AI; 1 IU/g feed) or high (HiD; 10 IU/g feed) D3 diet. Differences were considered significant at P ≤ 0.10, as this was a pilot study. Results: For paw grip endurance, HiD mice had a 7% greater score between 60‐133 d versus AI mice (P = 0.074). For motor performance, HiD mice had a 22% greater score between 60‐133 days (P = 0.074) versus AI mice due to changes observed in male mice, where HiD males had a 33% greater score (P = 0.064) versus AI males. There were no significant diet differences in disease onset, disease progression, or lifespan. Conclusion: Although disease outcomes were not affected, D3 supplementation at 10-fold the AI improved paw grip endurance and motor performance in the transgenic G93A mouse model of ALS, specifically in males.
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Dietary vitamin D3 at 50x the Adequate Intake increases apoptosis in the quadriceps of the female G93A mouse model of amyotrophic lateral sclerosis: a pilot study
The FASEB Journal, 2012Co-Authors: Egor Parkhomenko, Angelica Milionis, Alexandro Gianforcaro, Jesse Adam Solomon, Mazen J. HamadehAbstract:BackgroundWe previously showed that dietary vitamin D3 (D3) at 50x the Adequate Intake (AI) increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), ...
Stephen A Wise - One of the best experts on this subject based on the ideXlab platform.
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isotope dilution liquid chromatography mass spectrometry methods for fat and water soluble vitamins in nutritional formulations
Analytical Abstracts, 2011Co-Authors: Karen W Phinney, Catherine A Rimmer, Jeanice Brown M Thomas, Lane C Sander, Katherine E Sharpless, Stephen A WiseAbstract:Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have Adequate Intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient Intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography-mass spectrometry (LC-MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC-MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When possible, stable isotope labeled internal standards were employed for quantification.
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isotope dilution liquid chromatography mass spectrometry methods for fat and water soluble vitamins in nutritional formulations
Analytical Chemistry, 2011Co-Authors: Karen W Phinney, Catherine A Rimmer, Jeanice Brown M Thomas, Lane C Sander, Katherine E Sharpless, Stephen A WiseAbstract:Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have Adequate Intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient Intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography−mass spectrometry (LC−MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC−MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When po...
Alexandro Gianforcaro - One of the best experts on this subject based on the ideXlab platform.
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Vitamin D3 supplementation at 50x the Adequate Intake attenuates disease pathophysiology in the spinal cord of male, but is toxic in female, G93A mouse model of amyotrophic lateral sclerosis (ALS)
The FASEB Journal, 2015Co-Authors: Elnaz Moghimi, Alexandro Gianforcaro, Jesse Adam Solomon, Sanjeef Thampinathan, Mazen J. HamadehAbstract:Background: Vitamin D3 (D3) at 50x the Adequate Intake (AI) improves paw grip endurance in G93A mice. However, quadriceps apoptosis increases in females, indicating a threshold of toxicity. ALS is ...
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dietary vitamin d3 supplementation at 50 the Adequate Intake decreases calbindin d28k and endoplasmic reticulum stress and increases apoptosis suggesting toxicity in the female transgenic g93a mouse model of amyotrophic lateral sclerosis
The FASEB Journal, 2013Co-Authors: Siavash Taherishalmani, Alexandro Gianforcaro, Jesse Adam Solomon, Shayan Shahsavar, Mazen J. HamadehAbstract:BackgroundWe previously showed that dietary vitamin D3 (D3) at 50× the Adequate Intake increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), with ...
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vitamin d3 at 50x ai attenuates the decline in paw grip endurance but not disease outcomes in the g93a mouse model of als and is toxic in females
PLOS ONE, 2013Co-Authors: Alexandro Gianforcaro, Jesse Adam Solomon, Mazen J. HamadehAbstract:Background We previously demonstrated that dietary vitamin D3 at 10x the Adequate Intake (AI) attenuates the decline in functional capacity in the G93A mouse model of ALS. We hypothesized that higher doses would elicit more robust changes in functional and disease outcomes. Objective To determine the effects of dietary vitamin D3 at 50xAI on functional outcomes (motor performance, paw grip endurance) and disease severity (clinical score), as well as disease onset, disease progression and lifespan in the transgenic G93A mouse model of ALS. Methods Starting at age 25 d, 100 G93A mice (55 M, 45 F) were provided ad libitum with either an Adequate (AI; 1 IU D3/g feed) or high (HiD; 50 IU D3/g feed) vitamin D3 diet. Results HiD females consumed 9% less food corrected for body weight vs. AI females (P = 0.010). HiD mice had a 12% greater paw grip endurance over time between age 60–141 d (P = 0.015), and a 37% greater score during disease progression (P = 0.042) vs. AI mice. Although HiD females had a non-significant 31% greater CS prior to disease onset vs. AI females, they exhibited a significant 20% greater paw grip endurance AUC (P = 0.020) when corrected for clinical score. Conclusion Dietary D3 supplementation at 50x the Adequate Intake attenuated the decline in paw grip endurance, but did not influence age at disease onset, hindlimb paralysis or endpoint in the transgenic G93A mouse model of ALS. Furthermore, females may have reached the threshold for vitamin D3 toxicity as evidence by reduced food Intake and greater disease severity prior to disease onset.
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dietary vitamin d3 supplementation at 10 the Adequate Intake improves functional capacity in the g93a transgenic mouse model of als a pilot study
CNS Neuroscience & Therapeutics, 2012Co-Authors: Alexandro Gianforcaro, Mazen J. HamadehAbstract:SUMMARY Background: Vitamin D has antioxidant, anti-inflammatory, and neuroprotective properties, and may mitigate amyotrophic lateral sclerosis (ALS) pathology. Aims: To determine the effects of dietary vitamin D3 (D3) at 10-fold the Adequate Intake (AI) on functional and disease outcomes and lifespan in the transgenic G93A mouse model of ALS. Methods: Starting at age 40 days, 32 G93A mice (21 M, 11 F) were provided ad libitum with either an Adequate (AI; 1 IU/g feed) or high (HiD; 10 IU/g feed) D3 diet. Differences were considered significant at P ≤ 0.10, as this was a pilot study. Results: For paw grip endurance, HiD mice had a 7% greater score between 60‐133 d versus AI mice (P = 0.074). For motor performance, HiD mice had a 22% greater score between 60‐133 days (P = 0.074) versus AI mice due to changes observed in male mice, where HiD males had a 33% greater score (P = 0.064) versus AI males. There were no significant diet differences in disease onset, disease progression, or lifespan. Conclusion: Although disease outcomes were not affected, D3 supplementation at 10-fold the AI improved paw grip endurance and motor performance in the transgenic G93A mouse model of ALS, specifically in males.
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Dietary vitamin D3 at 50x the Adequate Intake increases apoptosis in the quadriceps of the female G93A mouse model of amyotrophic lateral sclerosis: a pilot study
The FASEB Journal, 2012Co-Authors: Egor Parkhomenko, Angelica Milionis, Alexandro Gianforcaro, Jesse Adam Solomon, Mazen J. HamadehAbstract:BackgroundWe previously showed that dietary vitamin D3 (D3) at 50x the Adequate Intake (AI) increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), ...
Karen W Phinney - One of the best experts on this subject based on the ideXlab platform.
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isotope dilution liquid chromatography mass spectrometry methods for fat and water soluble vitamins in nutritional formulations
Analytical Abstracts, 2011Co-Authors: Karen W Phinney, Catherine A Rimmer, Jeanice Brown M Thomas, Lane C Sander, Katherine E Sharpless, Stephen A WiseAbstract:Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have Adequate Intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient Intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography-mass spectrometry (LC-MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC-MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When possible, stable isotope labeled internal standards were employed for quantification.
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isotope dilution liquid chromatography mass spectrometry methods for fat and water soluble vitamins in nutritional formulations
Analytical Chemistry, 2011Co-Authors: Karen W Phinney, Catherine A Rimmer, Jeanice Brown M Thomas, Lane C Sander, Katherine E Sharpless, Stephen A WiseAbstract:Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have Adequate Intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient Intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography−mass spectrometry (LC−MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC−MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When po...
Jesse Adam Solomon - One of the best experts on this subject based on the ideXlab platform.
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Vitamin D3 supplementation at 50x the Adequate Intake attenuates disease pathophysiology in the spinal cord of male, but is toxic in female, G93A mouse model of amyotrophic lateral sclerosis (ALS)
The FASEB Journal, 2015Co-Authors: Elnaz Moghimi, Alexandro Gianforcaro, Jesse Adam Solomon, Sanjeef Thampinathan, Mazen J. HamadehAbstract:Background: Vitamin D3 (D3) at 50x the Adequate Intake (AI) improves paw grip endurance in G93A mice. However, quadriceps apoptosis increases in females, indicating a threshold of toxicity. ALS is ...
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dietary vitamin d3 supplementation at 50 the Adequate Intake decreases calbindin d28k and endoplasmic reticulum stress and increases apoptosis suggesting toxicity in the female transgenic g93a mouse model of amyotrophic lateral sclerosis
The FASEB Journal, 2013Co-Authors: Siavash Taherishalmani, Alexandro Gianforcaro, Jesse Adam Solomon, Shayan Shahsavar, Mazen J. HamadehAbstract:BackgroundWe previously showed that dietary vitamin D3 (D3) at 50× the Adequate Intake increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), with ...
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vitamin d3 at 50x ai attenuates the decline in paw grip endurance but not disease outcomes in the g93a mouse model of als and is toxic in females
PLOS ONE, 2013Co-Authors: Alexandro Gianforcaro, Jesse Adam Solomon, Mazen J. HamadehAbstract:Background We previously demonstrated that dietary vitamin D3 at 10x the Adequate Intake (AI) attenuates the decline in functional capacity in the G93A mouse model of ALS. We hypothesized that higher doses would elicit more robust changes in functional and disease outcomes. Objective To determine the effects of dietary vitamin D3 at 50xAI on functional outcomes (motor performance, paw grip endurance) and disease severity (clinical score), as well as disease onset, disease progression and lifespan in the transgenic G93A mouse model of ALS. Methods Starting at age 25 d, 100 G93A mice (55 M, 45 F) were provided ad libitum with either an Adequate (AI; 1 IU D3/g feed) or high (HiD; 50 IU D3/g feed) vitamin D3 diet. Results HiD females consumed 9% less food corrected for body weight vs. AI females (P = 0.010). HiD mice had a 12% greater paw grip endurance over time between age 60–141 d (P = 0.015), and a 37% greater score during disease progression (P = 0.042) vs. AI mice. Although HiD females had a non-significant 31% greater CS prior to disease onset vs. AI females, they exhibited a significant 20% greater paw grip endurance AUC (P = 0.020) when corrected for clinical score. Conclusion Dietary D3 supplementation at 50x the Adequate Intake attenuated the decline in paw grip endurance, but did not influence age at disease onset, hindlimb paralysis or endpoint in the transgenic G93A mouse model of ALS. Furthermore, females may have reached the threshold for vitamin D3 toxicity as evidence by reduced food Intake and greater disease severity prior to disease onset.
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Dietary vitamin D3 at 50x the Adequate Intake increases apoptosis in the quadriceps of the female G93A mouse model of amyotrophic lateral sclerosis: a pilot study
The FASEB Journal, 2012Co-Authors: Egor Parkhomenko, Angelica Milionis, Alexandro Gianforcaro, Jesse Adam Solomon, Mazen J. HamadehAbstract:BackgroundWe previously showed that dietary vitamin D3 (D3) at 50x the Adequate Intake (AI) increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), ...