The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform
N Fuzzati - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography electrospray ionization mass spectrometry and multiple mass spectrometry studies of hyperforin degradation products
Journal of Chromatography A, 2001Co-Authors: N Fuzzati, B Gabetta, I Strepponi, F VillaAbstract:The alcoholic extract of the aerial parts of Hypericum perforatum L. finds wide application because of its antidepressant activity. The extract contains a number of constituents with documented biological activity including chlorogenic acid, a broad range of flavonoids, naphthodianthrones and phloroglucinols. Hyperforin and Adhyperforin are the major phloroglucinol constituents found in the lipophilic fraction of the extracts. Since the stability of hyperforin has been shown to be limited, an investigation of the hyperforin degradation products using HPLC-electrospray ionization mass spectrometry and multiple mass spectrometry was undertaken.
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identification by high performance liquid chromatography diode array detection mass spectrometry and quantification by high performance liquid chromatography uv absorbance detection of active constituents of hypericum perforatum
Journal of Chromatography A, 1998Co-Authors: M Brolis, N Fuzzati, B Gabetta, R. Pace, F Panzeri, F PeterlongoAbstract:Abstract Hypericum perforatum is a medicinal plant which has been known in traditional medicine as an anti-inflammatory and healing agent. Nowadays, the alcoholic extract of its aerial parts finds wide application for its antidepressant activity. A high-performance liquid chromatography (HPLC) method for the identification of its constituents using a wide pore RP-18 column and a water–methanol–acetonitrile–phosphoric acid mobile phase system was developed. The identification of its flavonoid, naphthodianthrone and phloroglucinol constituents was performed using combined HPLC–diode array detection (DAD) analysis, HPLC–thermospray and HPLC–electrospray mass spectrometry. Chlorogenic acid, quercetin, quercitrin, isoquercitrin, rutin, hyperoside, I3,II8-biapigenin, pseudohypericin, hypericin, hyperforin and Adhyperforin were separated by an aqueous phosphoric acid–acetonitrile–methanol gradient within 50 min. The quantification of the above constituents was performed using rutin as an external standard.
Ioannis P Gerothanassis - One of the best experts on this subject based on the ideXlab platform.
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identification of the major constituents of hypericum perforatum by lc spe nmr and or lc ms
Phytochemistry, 2007Co-Authors: Evangelos C Tatsis, Sjef Boeren, Vassiliki Exarchou, Anastassios N Troganis, Jacques Vervoort, Ioannis P GerothanassisAbstract:Abstract The newly established hyphenated instrumentation of LC/DAD/SPE/NMR and LC/UV/(ESI)MS techniques have been applied for separation and structure verification of the major known constituents present in Greek Hypericum perforatum extracts. The chromatographic separation was performed on a C18 column. Acetonitrile-water was used as a mobile phase. For the on-line NMR detection, the analytes eluted from column were trapped one by one onto separate SPE cartridges, and hereafter transported into the NMR flow-cell. LC/DAD/SPE/NMR and LC/UV/MS allowed the characterization of constituents of Greek H. perforatum, mainly naphtodianthrones (hypericin, pseudohypericin, protohypericin, protopseudohypericin), phloroglucinols (hyperforin, Adhyperforin), flavonoids (quercetin, quercitrin, isoquercitrin, hyperoside, astilbin, miquelianin, I3,II8-biapigenin) and phenolic acids (chlorogenic acid, 3-O-coumaroylquinic acid). Two phloroglucinols (hyperfirin and adhyperfirin) were detected for the first time, which have been previously reported to be precursors in the biosynthesis of hyperforin and Adhyperforin.
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Identification of the major constituents of Hypericum perforatum by LC/SPE/NMR and/or LC/MS.
Phytochemistry, 2006Co-Authors: Evangelos C Tatsis, Sjef Boeren, Vassiliki Exarchou, Anastassios N Troganis, Jacques Vervoort, Ioannis P GerothanassisAbstract:Abstract The newly established hyphenated instrumentation of LC/DAD/SPE/NMR and LC/UV/(ESI)MS techniques have been applied for separation and structure verification of the major known constituents present in Greek Hypericum perforatum extracts. The chromatographic separation was performed on a C18 column. Acetonitrile-water was used as a mobile phase. For the on-line NMR detection, the analytes eluted from column were trapped one by one onto separate SPE cartridges, and hereafter transported into the NMR flow-cell. LC/DAD/SPE/NMR and LC/UV/MS allowed the characterization of constituents of Greek H. perforatum, mainly naphtodianthrones (hypericin, pseudohypericin, protohypericin, protopseudohypericin), phloroglucinols (hyperforin, Adhyperforin), flavonoids (quercetin, quercitrin, isoquercitrin, hyperoside, astilbin, miquelianin, I3,II8-biapigenin) and phenolic acids (chlorogenic acid, 3-O-coumaroylquinic acid). Two phloroglucinols (hyperfirin and adhyperfirin) were detected for the first time, which have been previously reported to be precursors in the biosynthesis of hyperforin and Adhyperforin.
F Villa - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography electrospray ionization mass spectrometry and multiple mass spectrometry studies of hyperforin degradation products
Journal of Chromatography A, 2001Co-Authors: N Fuzzati, B Gabetta, I Strepponi, F VillaAbstract:The alcoholic extract of the aerial parts of Hypericum perforatum L. finds wide application because of its antidepressant activity. The extract contains a number of constituents with documented biological activity including chlorogenic acid, a broad range of flavonoids, naphthodianthrones and phloroglucinols. Hyperforin and Adhyperforin are the major phloroglucinol constituents found in the lipophilic fraction of the extracts. Since the stability of hyperforin has been shown to be limited, an investigation of the hyperforin degradation products using HPLC-electrospray ionization mass spectrometry and multiple mass spectrometry was undertaken.
B Gabetta - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography electrospray ionization mass spectrometry and multiple mass spectrometry studies of hyperforin degradation products
Journal of Chromatography A, 2001Co-Authors: N Fuzzati, B Gabetta, I Strepponi, F VillaAbstract:The alcoholic extract of the aerial parts of Hypericum perforatum L. finds wide application because of its antidepressant activity. The extract contains a number of constituents with documented biological activity including chlorogenic acid, a broad range of flavonoids, naphthodianthrones and phloroglucinols. Hyperforin and Adhyperforin are the major phloroglucinol constituents found in the lipophilic fraction of the extracts. Since the stability of hyperforin has been shown to be limited, an investigation of the hyperforin degradation products using HPLC-electrospray ionization mass spectrometry and multiple mass spectrometry was undertaken.
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identification by high performance liquid chromatography diode array detection mass spectrometry and quantification by high performance liquid chromatography uv absorbance detection of active constituents of hypericum perforatum
Journal of Chromatography A, 1998Co-Authors: M Brolis, N Fuzzati, B Gabetta, R. Pace, F Panzeri, F PeterlongoAbstract:Abstract Hypericum perforatum is a medicinal plant which has been known in traditional medicine as an anti-inflammatory and healing agent. Nowadays, the alcoholic extract of its aerial parts finds wide application for its antidepressant activity. A high-performance liquid chromatography (HPLC) method for the identification of its constituents using a wide pore RP-18 column and a water–methanol–acetonitrile–phosphoric acid mobile phase system was developed. The identification of its flavonoid, naphthodianthrone and phloroglucinol constituents was performed using combined HPLC–diode array detection (DAD) analysis, HPLC–thermospray and HPLC–electrospray mass spectrometry. Chlorogenic acid, quercetin, quercitrin, isoquercitrin, rutin, hyperoside, I3,II8-biapigenin, pseudohypericin, hypericin, hyperforin and Adhyperforin were separated by an aqueous phosphoric acid–acetonitrile–methanol gradient within 50 min. The quantification of the above constituents was performed using rutin as an external standard.
Piergiorgio Pietta - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography electrospray mass spectrometry of hypericum perforatum extracts
Rapid Communications in Mass Spectrometry, 2000Co-Authors: Pierluigi Mauri, Piergiorgio PiettaAbstract:Hypericum perforatum L. (St. John's Wort) is a widely distributed herbaceous perennial plant which has been well known as a medicinal plant since antiquity. In recent years, H. perforatum has received increasing attention for the treatment of depression and other neuralgic disorders. The main constituents of H. perforatum extract include flavonoids, naphthodianthrones, phloroglucinols, essential oils and xanthones. The present work reports the analysis of naphthodianthrones and phloroglucinols in H. perforatum extracts by means of high performance liquid chromatography (HPLC) coupled simultaneously to a diode array detector (DAD) and electrospray mass spectrometry (ESI-MS). Hypericin, pseudohypericin, hyperforin and Adhyperforin were separated and identified on the base of their on-line UV and mass spectra. Quantitative analysis of hypericin derivatives in different extracts of H. perforatum using DAD and MS detectors was performed. In addition, direct infusion ESI-MS of H. perforatum extracts was applied to obtain rapid mass fingerprints of constituents present in the sample.