The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform

Jordi Oliver - One of the best experts on this subject based on the ideXlab platform.

  • Non-tumor Adjacent Tissue of advanced stage from CRC shows activated antioxidant response.
    Free radical biology & medicine, 2018
    Co-Authors: Reyniel Hernández-lópez, Margalida Torrens-mas, Daniel Gabriel Pons, Esther Falcó, Teresa Fernández, Javier M. Ibarra De La Rosa, Jorge Sastre-serra, Jordi Oliver, Pilar Roca
    Abstract:

    Abstract Colorectal cancer (CRC) is a leading cause of malignant cancer-related morbidity and mortality, with a higher incidence in developed countries and a high mortality rate mainly attributable to metastases. The aim of the present study was to determine the metabolic adaptations related to oxidative stress in tumor Tissue from advanced stages (III and IV) of CRC and whether they could be used as potential biomarkers for clinical applications. To tackle this aim, we have analyzed the protein expression levels related to oxidative stress and the enzymatic activities of MnSOD and catalase, comparing samples of non-tumor Adjacent Tissue and tumor Tissue of CRC patients in stages III and IV. The results showed no differences between stage III and IV in tumor Tissues for any of the proteins studied. However, some differences were found between samples of non-tumor Adjacent Tissue and tumor Tissue for some of the antioxidant enzymes. Overwhelmingly, the greatest differences were detected when comparing samples of non-tumor Adjacent Tissue from stage III and stage IV. To the best of our knowledge, this is the first study where differences between the non-tumor Adjacent Tissues of CRC patients from different cancer stages were determined. This study suggests that the parameters analyzed should be evaluated as biomarkers for the evolution of CRC. Furthermore, tumor Tissue status should not be of sole importance for the prognosis of CRC, as the non-tumor Adjacent Tissues could also merit consideration.

  • Differences in antioxidant enzymes in tumour Tissue and non-tumour Adjacent Tissue in colorectal cancer patients at different stages (III and IV)
    Free Radical Biology and Medicine, 2017
    Co-Authors: Reyniel Hernández-lópez, Margalida Torrens-mas, Daniel Gabriel Pons, Esther Falcó, Teresa Fernández, Javier M. Ibarra De La Rosa, Jorge Sastre-serra, Pilar Roca, Jordi Oliver
    Abstract:

    Colorectal Cancer (CRC) is one of the leading causes of death by cancer worldwide; its high mortality rate is mainly attributable to metastases. Oxidative stress has been classically related to cancer due to the harmful effects of free radicals, both in their onset and in their evolution. Recent studies have revealed the influence of non-tumour Adjacent Tissue on the progression and metastasis of CRC. Based on this, we have studied the protein levels of antioxidant enzymes by western blot comparing samples of non-tumour Adjacent Tissue and tumour Tissue of CRC patients in advanced stages (III and IV). In the results obtained, higher levels of antioxidant enzymes have been observed in the tumour Tissue than in the non-tumour Adjacent Tissue, although these differences are shortened in stage IV, due to the fact that stage IV patients present a significant increase in levels of antioxidant enzymes in non-tumour Adjacent Tissue. In addition, higher levels of antioxidant enzymes have been observed in the non-tumour Adjacent Tissue of patients in IV stage than in III This study emphasizes the importance of non-tumour Adjacent Tissue as a prognostic factor in the evolution of CRC cancer patients.

Reyniel Hernández-lópez - One of the best experts on this subject based on the ideXlab platform.

  • Non-tumor Adjacent Tissue of advanced stage from CRC shows activated antioxidant response.
    Free radical biology & medicine, 2018
    Co-Authors: Reyniel Hernández-lópez, Margalida Torrens-mas, Daniel Gabriel Pons, Esther Falcó, Teresa Fernández, Javier M. Ibarra De La Rosa, Jorge Sastre-serra, Jordi Oliver, Pilar Roca
    Abstract:

    Abstract Colorectal cancer (CRC) is a leading cause of malignant cancer-related morbidity and mortality, with a higher incidence in developed countries and a high mortality rate mainly attributable to metastases. The aim of the present study was to determine the metabolic adaptations related to oxidative stress in tumor Tissue from advanced stages (III and IV) of CRC and whether they could be used as potential biomarkers for clinical applications. To tackle this aim, we have analyzed the protein expression levels related to oxidative stress and the enzymatic activities of MnSOD and catalase, comparing samples of non-tumor Adjacent Tissue and tumor Tissue of CRC patients in stages III and IV. The results showed no differences between stage III and IV in tumor Tissues for any of the proteins studied. However, some differences were found between samples of non-tumor Adjacent Tissue and tumor Tissue for some of the antioxidant enzymes. Overwhelmingly, the greatest differences were detected when comparing samples of non-tumor Adjacent Tissue from stage III and stage IV. To the best of our knowledge, this is the first study where differences between the non-tumor Adjacent Tissues of CRC patients from different cancer stages were determined. This study suggests that the parameters analyzed should be evaluated as biomarkers for the evolution of CRC. Furthermore, tumor Tissue status should not be of sole importance for the prognosis of CRC, as the non-tumor Adjacent Tissues could also merit consideration.

  • Differences in antioxidant enzymes in tumour Tissue and non-tumour Adjacent Tissue in colorectal cancer patients at different stages (III and IV)
    Free Radical Biology and Medicine, 2017
    Co-Authors: Reyniel Hernández-lópez, Margalida Torrens-mas, Daniel Gabriel Pons, Esther Falcó, Teresa Fernández, Javier M. Ibarra De La Rosa, Jorge Sastre-serra, Pilar Roca, Jordi Oliver
    Abstract:

    Colorectal Cancer (CRC) is one of the leading causes of death by cancer worldwide; its high mortality rate is mainly attributable to metastases. Oxidative stress has been classically related to cancer due to the harmful effects of free radicals, both in their onset and in their evolution. Recent studies have revealed the influence of non-tumour Adjacent Tissue on the progression and metastasis of CRC. Based on this, we have studied the protein levels of antioxidant enzymes by western blot comparing samples of non-tumour Adjacent Tissue and tumour Tissue of CRC patients in advanced stages (III and IV). In the results obtained, higher levels of antioxidant enzymes have been observed in the tumour Tissue than in the non-tumour Adjacent Tissue, although these differences are shortened in stage IV, due to the fact that stage IV patients present a significant increase in levels of antioxidant enzymes in non-tumour Adjacent Tissue. In addition, higher levels of antioxidant enzymes have been observed in the non-tumour Adjacent Tissue of patients in IV stage than in III This study emphasizes the importance of non-tumour Adjacent Tissue as a prognostic factor in the evolution of CRC cancer patients.

Pilar Roca - One of the best experts on this subject based on the ideXlab platform.

  • Non-tumor Adjacent Tissue of advanced stage from CRC shows activated antioxidant response.
    Free radical biology & medicine, 2018
    Co-Authors: Reyniel Hernández-lópez, Margalida Torrens-mas, Daniel Gabriel Pons, Esther Falcó, Teresa Fernández, Javier M. Ibarra De La Rosa, Jorge Sastre-serra, Jordi Oliver, Pilar Roca
    Abstract:

    Abstract Colorectal cancer (CRC) is a leading cause of malignant cancer-related morbidity and mortality, with a higher incidence in developed countries and a high mortality rate mainly attributable to metastases. The aim of the present study was to determine the metabolic adaptations related to oxidative stress in tumor Tissue from advanced stages (III and IV) of CRC and whether they could be used as potential biomarkers for clinical applications. To tackle this aim, we have analyzed the protein expression levels related to oxidative stress and the enzymatic activities of MnSOD and catalase, comparing samples of non-tumor Adjacent Tissue and tumor Tissue of CRC patients in stages III and IV. The results showed no differences between stage III and IV in tumor Tissues for any of the proteins studied. However, some differences were found between samples of non-tumor Adjacent Tissue and tumor Tissue for some of the antioxidant enzymes. Overwhelmingly, the greatest differences were detected when comparing samples of non-tumor Adjacent Tissue from stage III and stage IV. To the best of our knowledge, this is the first study where differences between the non-tumor Adjacent Tissues of CRC patients from different cancer stages were determined. This study suggests that the parameters analyzed should be evaluated as biomarkers for the evolution of CRC. Furthermore, tumor Tissue status should not be of sole importance for the prognosis of CRC, as the non-tumor Adjacent Tissues could also merit consideration.

  • Differences in antioxidant enzymes in tumour Tissue and non-tumour Adjacent Tissue in colorectal cancer patients at different stages (III and IV)
    Free Radical Biology and Medicine, 2017
    Co-Authors: Reyniel Hernández-lópez, Margalida Torrens-mas, Daniel Gabriel Pons, Esther Falcó, Teresa Fernández, Javier M. Ibarra De La Rosa, Jorge Sastre-serra, Pilar Roca, Jordi Oliver
    Abstract:

    Colorectal Cancer (CRC) is one of the leading causes of death by cancer worldwide; its high mortality rate is mainly attributable to metastases. Oxidative stress has been classically related to cancer due to the harmful effects of free radicals, both in their onset and in their evolution. Recent studies have revealed the influence of non-tumour Adjacent Tissue on the progression and metastasis of CRC. Based on this, we have studied the protein levels of antioxidant enzymes by western blot comparing samples of non-tumour Adjacent Tissue and tumour Tissue of CRC patients in advanced stages (III and IV). In the results obtained, higher levels of antioxidant enzymes have been observed in the tumour Tissue than in the non-tumour Adjacent Tissue, although these differences are shortened in stage IV, due to the fact that stage IV patients present a significant increase in levels of antioxidant enzymes in non-tumour Adjacent Tissue. In addition, higher levels of antioxidant enzymes have been observed in the non-tumour Adjacent Tissue of patients in IV stage than in III This study emphasizes the importance of non-tumour Adjacent Tissue as a prognostic factor in the evolution of CRC cancer patients.

Delphine J Lee - One of the best experts on this subject based on the ideXlab platform.

  • beyond the immediate tumor microenvironment distinct t cell phenotype and function of tumor Adjacent healthy breast Tissue associated with regional metastasis in breast cancer
    Journal for ImmunoTherapy of Cancer, 2014
    Co-Authors: Yi Guo, Alfred A Chan, Ingrid Cely, Jaime Shamonki, Michelle Kinnaird, Maggie Dinome, Begonya Cominanduix, Peter A Sieling, Delphine J Lee
    Abstract:

    While current breast cancer staging includes lymph node involvement as a key indicator of prognosis, little is known about how healthy Tissue surrounding the immediate tumor microenvironment contributes to the loco-regional spread of disease. Previous studies of the role of the immune microenvironment in breast cancer have focused on the study of intratumoral T cells, reporting that increased intratumoral CD8+ T cell proportions with decreased CD4+ T cell proportions are associated with lymph node metastasis and rapid tumor progression. We hypothesized that T cells within the unaffected breast Tissue ("healthy-Adjacent" Tissue) near the tumor (2-5 cm away from the tumor margin) may influence the local/regional spread of tumor cells. Since the amount of Tissue available for study from the excision of primary tumors is limited, we utilized a previously established three-dimensional explant method to expand resident T cells from fresh breast cancer and healthy-Adjacent breast Tissue to investigate their phenotypic and functional qualities. We compared Tissues from patients with LN metastasis (n = 8) and those without LN involvement (n = 7). We defined the surface phenotype of T cells derived from breast cancer and healthy-Adjacent Tissue using multiparameter flow cytometry. We found that healthy-Adjacent Tissue from patients with LN metastases exhibited a 1.6 fold lower percentage of CD4 T cells (p < 0.01) and 3.7 fold higher percentage of CD8 T cells (p < 0.05) than patients with no LN involvement. In contrast, we did not find any statistically significant differences in the number of intratumoral CD4 and CD8 T cells in patients with LN metastases versus those with no LN involvement. We hypothesized that qualitative T cell cytokine response from the surrounding healthy Adjacent Tissue may also be associated with LN metastases. Our preliminary data from the study of breast cancer patients (n = 6) showed some trends of increased Th2 cytokine and decreased pro-inflammatory cytokine associated with nodal metastases. These data suggest that the Tissue extending beyond the tumor microenvironment (healthy-Adjacent Tissue) may contribute to immune surveillance and regional metastases. While this study size is small, it demonstrates the feasibility of using the explant method to interrogate healthy-appearing tumor Adjacent Tissue to investigate the surrounding breast Tissue environment. Our preliminary findings also indicate the potential relevance of investigating the role of T cells in tumor Adjacent healthy-appearing breast Tissue to provide tumor immune surveillance. Further analysis of the T cells present in healthy Adjacent breast Tissue is warranted.

Li Shan - One of the best experts on this subject based on the ideXlab platform.

  • Low claudin-6 expression correlates with poor prognosis in patients with non-small cell lung cancer.
    OncoTargets and therapy, 2015
    Co-Authors: Qiang Wang, Yan Zhang, Tao Zhang, Zhigang Han, Li Shan
    Abstract:

    OBJECTIVE: Claudins are found in junctional complexes mediating cell adhesion and are involved in the attachment of tight junctions to the underlying cytoskeleton. Abnormal claudin-6 expression has been observed for a variety of malignant solid tumors, but the expression of claudin-6 in non-small cell lung cancer (NSCLC) has not yet been characterized. METHODS: Immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and western blot analysis were used to quantify claudin-6 expression in 123 cases of NSCLC and non-cancerous Adjacent Tissue. We analyzed the relationship between claudin-6 expression and clinicopathological features of NSCLC. The Kaplan-Meier method was used to analyze postoperative survival rates, and the log-rank test was used to assess differences in survival rates. The Cox regression model was used to perform multivariate analysis. RESULTS: Claudin-6 expression was low for 61 of 123 (49.6%) NSCLC Tissue samples and for 33 of 123 (26.8%) normal Adjacent Tissue samples. RT-PCR and western blot analyses confirmed the immunohistochemistry results. Claudin-6 expression was associated with lymph node metastasis (P