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Zhu Wang - One of the best experts on this subject based on the ideXlab platform.
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effect of qufeng tongluo recipe on expression of desmin and cd2ap proteins in adriamycin induced Nephropathy rats an experimental research
Chinese journal of integrated traditional and Western medicine, 2014Co-Authors: Zhu Wang, Ruiping Li, Ye WangAbstract:OBJECTIVE: To investigate the effect of Qufeng Tongluo Recipe (QTR) on the expression of desmin and CD2-associated protein (CD2AP) in Adriamycin-Induced Nephropathy rats. METHODS: The Adriamycin-Induced Nephropathy rat model was induced by a disposable intravenous injection of adriamycin. The model was successfully established after 3 weeks. Rats were then randomly divided into the blank control group (Group A, n =12), the model control group (Group B, n = 8), the small, medium, large dose QTR group (Group C, n = 8; Group D, n = 8; Group E, n = 8), and the positive control group (Group F, n = 8). From the fourth week normal saline was given to rats in Group A and Group B, QTR 1.0 g/mL, 2.1 g/mL, and 4.2 g/mL was respectively administered to those in Group C, D, and E. Prednisone 25 mg/kg was given to rats in Group F. All medication was performed by gastrogavage at 10 mL/kg, once daily, for 28 successive days. 24-h urinary protein excretion and sera biochemical indices were determined during medication. At the end of the experiment, ultrastructure was observed, mRNA expression of desmin, mRNA and protein of CD2AP were detected by Real-time PCR and Western blot. RESULTS: (1) Compared with Group B, 24-h urinary protein excretion significantly decreased in Group C, D, E, and F (P < 0.05). (2) Compared with Group B, Alb in Group C, D, and E increased (P < 0.05) and TC significantly decreased (P < 0.05). TG significantly increased in Group F (P < 0.05). (3) Results of electron microscope showed, compared with Group B, the morphology of foot cells was improved to various degrees in Groups D, E, and F, especially the foot process structure and the number of foot processes were significantly improved, which was more obviously shown in Group D and Group E. (4) mRNA expression of desmin, mRNA and protein of CD2AP increased in Adriamycin-Induced Nephropathy rats (P < 0.05). After intervention, when compared with Group B, mRNA expression of desmin and CD2AP were significantly lower in Group C, D, E, and F (P < 0.05). (5) Compared with Group A, expression of desmin and CD2AP significantly increased (P < 0.05). Compared with Group B, the expression of desmin protein were obviously lower in Group C, D, E, and F, and the protein expression of desmin obviously decreased in Group D, E, and F (P < 0.05). The protein expression of desmin and CD2AP gradually decreased in Group C, D, and E (P < 0.05). Compared with Group F, the expression of CD2AP protein obviously increased in Group C and D (P < 0.05); the expression of CD2AP protein obviously decreased in Group E (P < 0.05); the expression of desmin protein was higher in Group C, D, and E (P < 0.05). CONCLUSION: QTR's therapeutic effect on Adriamycin-Induced Nephropathy rats might be achieved through altered expression of desmin and CD2AP.
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effect of qufeng tongluo recipe on expression of desmin and cd2ap proteins in adriamycin induced Nephropathy rats an experimental research
Chinese journal of integrated traditional and Western medicine, 2014Co-Authors: Zhu Wang, Ruiping Li, Ye WangAbstract:OBJECTIVE: To investigate the effect of Qufeng Tongluo Recipe (QTR) on the expression of desmin and CD2-associated protein (CD2AP) in Adriamycin-Induced Nephropathy rats. METHODS: The Adriamycin-Induced Nephropathy rat model was induced by a disposable intravenous injection of adriamycin. The model was successfully established after 3 weeks. Rats were then randomly divided into the blank control group (Group A, n =12), the model control group (Group B, n = 8), the small, medium, large dose QTR group (Group C, n = 8; Group D, n = 8; Group E, n = 8), and the positive control group (Group F, n = 8). From the fourth week normal saline was given to rats in Group A and Group B, QTR 1.0 g/mL, 2.1 g/mL, and 4.2 g/mL was respectively administered to those in Group C, D, and E. Prednisone 25 mg/kg was given to rats in Group F. All medication was performed by gastrogavage at 10 mL/kg, once daily, for 28 successive days. 24-h urinary protein excretion and sera biochemical indices were determined during medication. At the end of the experiment, ultrastructure was observed, mRNA expression of desmin, mRNA and protein of CD2AP were detected by Real-time PCR and Western blot. RESULTS: (1) Compared with Group B, 24-h urinary protein excretion significantly decreased in Group C, D, E, and F (P < 0.05). (2) Compared with Group B, Alb in Group C, D, and E increased (P < 0.05) and TC significantly decreased (P < 0.05). TG significantly increased in Group F (P < 0.05). (3) Results of electron microscope showed, compared with Group B, the morphology of foot cells was improved to various degrees in Groups D, E, and F, especially the foot process structure and the number of foot processes were significantly improved, which was more obviously shown in Group D and Group E. (4) mRNA expression of desmin, mRNA and protein of CD2AP increased in Adriamycin-Induced Nephropathy rats (P < 0.05). After intervention, when compared with Group B, mRNA expression of desmin and CD2AP were significantly lower in Group C, D, E, and F (P < 0.05). (5) Compared with Group A, expression of desmin and CD2AP significantly increased (P < 0.05). Compared with Group B, the expression of desmin protein were obviously lower in Group C, D, E, and F, and the protein expression of desmin obviously decreased in Group D, E, and F (P < 0.05). The protein expression of desmin and CD2AP gradually decreased in Group C, D, and E (P < 0.05). Compared with Group F, the expression of CD2AP protein obviously increased in Group C and D (P < 0.05); the expression of CD2AP protein obviously decreased in Group E (P < 0.05); the expression of desmin protein was higher in Group C, D, and E (P < 0.05). CONCLUSION: QTR's therapeutic effect on Adriamycin-Induced Nephropathy rats might be achieved through altered expression of desmin and CD2AP.
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Effects of Qufengtongluo recipe on proteinuria and glomerular filtration membrane in rats with Adriamycin-Induced Nephropathy
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2011Co-Authors: Wan-sen Sun, Yan-yun Ren, Zhu WangAbstract:OBJECTIVE To assess the therapeutic effect of Qufengtongluo (QFTL) recipe against proteinuria and glomerular filtration membrane damage in rats with Adriamycin-Induced Nephropathy (AN). METHODS Fifty-six SD rats were randomized into normal control (A) and AN model groups. In the AN model group, the rat AN models established by a single intravenous injection of adriamycin via the tail vein were subdivided into model (B), QFTL recipe (C), prednisone (D), and benazepril (E) groups 3 weeks after adriamycin injection. The 24-h urinary protein level was measured and the expression of anionic sites on the filtration membrane was evaluated using electron microscope with PEI staining. Nephrin expression on the glomerular filtration membrane was detected with indirect immunofluorescence assay. RESULTS Compared with group A, the model group showed significantly increased level of 24-h urinary protein (P
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Effect of Qufengtongluo recipe on expression of nephrin mRNA in Adriamycin-Induced Nephropathy in rats
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2009Co-Authors: Yan-yun Ren, Yan-long Zhao, Wan-sen Sun, Zhu WangAbstract:OBJECTIVE To investigate the expression of nephrin mRNA in Adriamycin-Induced Nephropathy (AN) in rats, and to explore the effect of Qufengtongluo recipe on proteinuria in AN rats and on the expression of nephrin mRNA. METHODS Adriamycin Nephropathy was induced by a single tail intravenous injection of adriamycin. We randomly divided 140 rats into a normal control group (n=32) and a Nephropathy model group (n=108). Three weeks later, 90 AN rats were randomly divided into 5 groups: a model group, a qufeng group, a qufeng and prednisone group, a prednisone group, and a benazepri group (18 rats in each group). They were treated respectively, and renal tissue samples were collected at week 0, 3, 5, and 7, respectively. The distribution and expression of nephrin mRNA were examined by indirect immunofluorescence and semi-quantity RT-PCR. RESULTS In the AN rats, the diffuse fusion and effacement of foot processes were observed at week 3. The fluorescence intensity of nephrin and the expression of nephrin mRNA significantly increased in the qufeng group and the prednisone group compared with the model group at the week 7 (P
Ye Wang - One of the best experts on this subject based on the ideXlab platform.
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Vincristine alleviates Adriamycin-Induced Nephropathy through stabilizing actin cytoskeleton.
Cell & Bioscience, 2017Co-Authors: Lei Yin, Ye Wang, Youying Mao, Hejie Song, Wei Zhou, Zhen ZhangAbstract:Antimicrotubule agent vincristine (VCR) has long been known as an alternative treatment for frequent relapse nephrotic syndrome and steroid-dependent nephrotic syndrome (SDNS). However, the mechanism is unknown. Here we found that VCR at a dosage much lower than that as an antimicrotubule agent can alleviate adriamycin (ADR)-induced proteinuria and podocyte foot process effacement. In cultured podocytes, VCR prevents ADR-induced actin fiber disorganization. In both in vitro and in vivo models, VCR suppresses ADR-induced overexpression of α3β1 integrin and focal adhesion kinase (FAK). These data suggest that VCR may relieve ADR-induced Nephropathy through inhibiting injury-induced activation of integrin outside-in signaling to prevent actin cytoskeleton remodeling. Hence, our work reveals a novel role of VCR in regulating actin fiber assembly and provides first evidence on the therapeutic mechanism of VCR on nephrotic syndrome.
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effect of qufeng tongluo recipe on expression of desmin and cd2ap proteins in adriamycin induced Nephropathy rats an experimental research
Chinese journal of integrated traditional and Western medicine, 2014Co-Authors: Zhu Wang, Ruiping Li, Ye WangAbstract:OBJECTIVE: To investigate the effect of Qufeng Tongluo Recipe (QTR) on the expression of desmin and CD2-associated protein (CD2AP) in Adriamycin-Induced Nephropathy rats. METHODS: The Adriamycin-Induced Nephropathy rat model was induced by a disposable intravenous injection of adriamycin. The model was successfully established after 3 weeks. Rats were then randomly divided into the blank control group (Group A, n =12), the model control group (Group B, n = 8), the small, medium, large dose QTR group (Group C, n = 8; Group D, n = 8; Group E, n = 8), and the positive control group (Group F, n = 8). From the fourth week normal saline was given to rats in Group A and Group B, QTR 1.0 g/mL, 2.1 g/mL, and 4.2 g/mL was respectively administered to those in Group C, D, and E. Prednisone 25 mg/kg was given to rats in Group F. All medication was performed by gastrogavage at 10 mL/kg, once daily, for 28 successive days. 24-h urinary protein excretion and sera biochemical indices were determined during medication. At the end of the experiment, ultrastructure was observed, mRNA expression of desmin, mRNA and protein of CD2AP were detected by Real-time PCR and Western blot. RESULTS: (1) Compared with Group B, 24-h urinary protein excretion significantly decreased in Group C, D, E, and F (P < 0.05). (2) Compared with Group B, Alb in Group C, D, and E increased (P < 0.05) and TC significantly decreased (P < 0.05). TG significantly increased in Group F (P < 0.05). (3) Results of electron microscope showed, compared with Group B, the morphology of foot cells was improved to various degrees in Groups D, E, and F, especially the foot process structure and the number of foot processes were significantly improved, which was more obviously shown in Group D and Group E. (4) mRNA expression of desmin, mRNA and protein of CD2AP increased in Adriamycin-Induced Nephropathy rats (P < 0.05). After intervention, when compared with Group B, mRNA expression of desmin and CD2AP were significantly lower in Group C, D, E, and F (P < 0.05). (5) Compared with Group A, expression of desmin and CD2AP significantly increased (P < 0.05). Compared with Group B, the expression of desmin protein were obviously lower in Group C, D, E, and F, and the protein expression of desmin obviously decreased in Group D, E, and F (P < 0.05). The protein expression of desmin and CD2AP gradually decreased in Group C, D, and E (P < 0.05). Compared with Group F, the expression of CD2AP protein obviously increased in Group C and D (P < 0.05); the expression of CD2AP protein obviously decreased in Group E (P < 0.05); the expression of desmin protein was higher in Group C, D, and E (P < 0.05). CONCLUSION: QTR's therapeutic effect on Adriamycin-Induced Nephropathy rats might be achieved through altered expression of desmin and CD2AP.
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effect of qufeng tongluo recipe on expression of desmin and cd2ap proteins in adriamycin induced Nephropathy rats an experimental research
Chinese journal of integrated traditional and Western medicine, 2014Co-Authors: Zhu Wang, Ruiping Li, Ye WangAbstract:OBJECTIVE: To investigate the effect of Qufeng Tongluo Recipe (QTR) on the expression of desmin and CD2-associated protein (CD2AP) in Adriamycin-Induced Nephropathy rats. METHODS: The Adriamycin-Induced Nephropathy rat model was induced by a disposable intravenous injection of adriamycin. The model was successfully established after 3 weeks. Rats were then randomly divided into the blank control group (Group A, n =12), the model control group (Group B, n = 8), the small, medium, large dose QTR group (Group C, n = 8; Group D, n = 8; Group E, n = 8), and the positive control group (Group F, n = 8). From the fourth week normal saline was given to rats in Group A and Group B, QTR 1.0 g/mL, 2.1 g/mL, and 4.2 g/mL was respectively administered to those in Group C, D, and E. Prednisone 25 mg/kg was given to rats in Group F. All medication was performed by gastrogavage at 10 mL/kg, once daily, for 28 successive days. 24-h urinary protein excretion and sera biochemical indices were determined during medication. At the end of the experiment, ultrastructure was observed, mRNA expression of desmin, mRNA and protein of CD2AP were detected by Real-time PCR and Western blot. RESULTS: (1) Compared with Group B, 24-h urinary protein excretion significantly decreased in Group C, D, E, and F (P < 0.05). (2) Compared with Group B, Alb in Group C, D, and E increased (P < 0.05) and TC significantly decreased (P < 0.05). TG significantly increased in Group F (P < 0.05). (3) Results of electron microscope showed, compared with Group B, the morphology of foot cells was improved to various degrees in Groups D, E, and F, especially the foot process structure and the number of foot processes were significantly improved, which was more obviously shown in Group D and Group E. (4) mRNA expression of desmin, mRNA and protein of CD2AP increased in Adriamycin-Induced Nephropathy rats (P < 0.05). After intervention, when compared with Group B, mRNA expression of desmin and CD2AP were significantly lower in Group C, D, E, and F (P < 0.05). (5) Compared with Group A, expression of desmin and CD2AP significantly increased (P < 0.05). Compared with Group B, the expression of desmin protein were obviously lower in Group C, D, E, and F, and the protein expression of desmin obviously decreased in Group D, E, and F (P < 0.05). The protein expression of desmin and CD2AP gradually decreased in Group C, D, and E (P < 0.05). Compared with Group F, the expression of CD2AP protein obviously increased in Group C and D (P < 0.05); the expression of CD2AP protein obviously decreased in Group E (P < 0.05); the expression of desmin protein was higher in Group C, D, and E (P < 0.05). CONCLUSION: QTR's therapeutic effect on Adriamycin-Induced Nephropathy rats might be achieved through altered expression of desmin and CD2AP.
Yan-yun Ren - One of the best experts on this subject based on the ideXlab platform.
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Effects of Qufengtongluo Recipe on podocin mRNA expression and podocyte morphology in rats with Adriamycin-Induced Nephropathy
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2011Co-Authors: Wan-sen Sun, Yan-long Zhao, Yan-yun RenAbstract:OBJECTIVE: To observe the effects of Qufengtongluo Recipe (QFTLR) on the expression of podocin mRNA and podocyte morphology in rats with Adriamycin-Induced Nephropathy (AN), and explore the possible mechanism mediating the therapeutic effect of QFTLR on nephropathic proteinuria. METHODS: SD rats were randomized into normal control group, AN model group (established by a single injection of adriamycin via the tail vein), and 3 intervention groups with QFTLR, prednisone, or benazepril treatment. After the corresponding treatments, the expression of podocin mRNA in the renal tissues was detected by RT-PCR methods, and the morphological changes of the podocytes were examined by electron microscope. RESULTS: Compared with the normal control group, the AN model group showed significantly lowered expressions of podocin mRNA (P
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Effects of Qufengtongluo recipe on proteinuria and glomerular filtration membrane in rats with Adriamycin-Induced Nephropathy
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2011Co-Authors: Wan-sen Sun, Yan-yun Ren, Zhu WangAbstract:OBJECTIVE To assess the therapeutic effect of Qufengtongluo (QFTL) recipe against proteinuria and glomerular filtration membrane damage in rats with Adriamycin-Induced Nephropathy (AN). METHODS Fifty-six SD rats were randomized into normal control (A) and AN model groups. In the AN model group, the rat AN models established by a single intravenous injection of adriamycin via the tail vein were subdivided into model (B), QFTL recipe (C), prednisone (D), and benazepril (E) groups 3 weeks after adriamycin injection. The 24-h urinary protein level was measured and the expression of anionic sites on the filtration membrane was evaluated using electron microscope with PEI staining. Nephrin expression on the glomerular filtration membrane was detected with indirect immunofluorescence assay. RESULTS Compared with group A, the model group showed significantly increased level of 24-h urinary protein (P
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Effects of qufengtongluo recipe on expressions of HSPG mRNA and protein in Adriamycin-Induced Nephropathy rats
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2010Co-Authors: Wan-sen Sun, Yan-yun Ren, Yan-long ZhaoAbstract:OBJECTIVE To investigate the expression of HSPG in glomerular base membrane of Adriamycin-Induced Nephropathy (AN) rats, and the effect of Qufengtongluo recipe on HSPG mRNA expression and proteinuria in AN rats. METHODS One hundred forty rats were used in this study, including 32 rats in normal control group. AN was induced in the left rats by a single tail intravenous injection of adriamycin. Three weeks later, 90 AN rats were randomly divided into five groups; the Nephropathy group (B, n=18), the Qufeng group (C, n=18), Qufeng and prednisone group (D, n=18), prednisone group(E,n=18) and benazepri group (F, n= 18). The rats in these five groups were treated with different combination of Qufeng recipe and prednisone. In each group, renal tissue samples were collected at week 3 and 7. The distribution, expression of HSPG was examined by indirect immunofluorescence, and semi-quantity RT-PCR, respectively. RESULTS (1) In AN rats, the diffuse fusion and effacement of foot processes were observed when model established. (2) Compared with Nephropathy group, the average fluorescence intensity of HSPG dramatically increased in Qufeng group and prednisone group (P < 0.01), similarly, it also increased in D and F groups (P < 0.01). (3) Compared with Nephropathy group, the expression of HSPG mRNA was significantly up-regulated in other groups. (P < 0.01), especially in C and F groups. There was significant negative correlation between the expression of HSPG and quantity of 24-hour proteinuria. CONCLUSION The abnormal expression of HSPG and their altered distributions may be an important molecular mechanism that leads to the occurrence and development of proteinuria in AN rats. The effect of Qufengtongluo recipe on nephrotic syndrome might be related to the alteration of HSPG expression and distribution in glomerulus.
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Effect of Qufengtongluo recipe on expression of nephrin mRNA in Adriamycin-Induced Nephropathy in rats
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2009Co-Authors: Yan-yun Ren, Yan-long Zhao, Wan-sen Sun, Zhu WangAbstract:OBJECTIVE To investigate the expression of nephrin mRNA in Adriamycin-Induced Nephropathy (AN) in rats, and to explore the effect of Qufengtongluo recipe on proteinuria in AN rats and on the expression of nephrin mRNA. METHODS Adriamycin Nephropathy was induced by a single tail intravenous injection of adriamycin. We randomly divided 140 rats into a normal control group (n=32) and a Nephropathy model group (n=108). Three weeks later, 90 AN rats were randomly divided into 5 groups: a model group, a qufeng group, a qufeng and prednisone group, a prednisone group, and a benazepri group (18 rats in each group). They were treated respectively, and renal tissue samples were collected at week 0, 3, 5, and 7, respectively. The distribution and expression of nephrin mRNA were examined by indirect immunofluorescence and semi-quantity RT-PCR. RESULTS In the AN rats, the diffuse fusion and effacement of foot processes were observed at week 3. The fluorescence intensity of nephrin and the expression of nephrin mRNA significantly increased in the qufeng group and the prednisone group compared with the model group at the week 7 (P
Wan-sen Sun - One of the best experts on this subject based on the ideXlab platform.
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Effects of Qufengtongluo Recipe on podocin mRNA expression and podocyte morphology in rats with Adriamycin-Induced Nephropathy
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2011Co-Authors: Wan-sen Sun, Yan-long Zhao, Yan-yun RenAbstract:OBJECTIVE: To observe the effects of Qufengtongluo Recipe (QFTLR) on the expression of podocin mRNA and podocyte morphology in rats with Adriamycin-Induced Nephropathy (AN), and explore the possible mechanism mediating the therapeutic effect of QFTLR on nephropathic proteinuria. METHODS: SD rats were randomized into normal control group, AN model group (established by a single injection of adriamycin via the tail vein), and 3 intervention groups with QFTLR, prednisone, or benazepril treatment. After the corresponding treatments, the expression of podocin mRNA in the renal tissues was detected by RT-PCR methods, and the morphological changes of the podocytes were examined by electron microscope. RESULTS: Compared with the normal control group, the AN model group showed significantly lowered expressions of podocin mRNA (P
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Effects of Qufengtongluo recipe on proteinuria and glomerular filtration membrane in rats with Adriamycin-Induced Nephropathy
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2011Co-Authors: Wan-sen Sun, Yan-yun Ren, Zhu WangAbstract:OBJECTIVE To assess the therapeutic effect of Qufengtongluo (QFTL) recipe against proteinuria and glomerular filtration membrane damage in rats with Adriamycin-Induced Nephropathy (AN). METHODS Fifty-six SD rats were randomized into normal control (A) and AN model groups. In the AN model group, the rat AN models established by a single intravenous injection of adriamycin via the tail vein were subdivided into model (B), QFTL recipe (C), prednisone (D), and benazepril (E) groups 3 weeks after adriamycin injection. The 24-h urinary protein level was measured and the expression of anionic sites on the filtration membrane was evaluated using electron microscope with PEI staining. Nephrin expression on the glomerular filtration membrane was detected with indirect immunofluorescence assay. RESULTS Compared with group A, the model group showed significantly increased level of 24-h urinary protein (P
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Effects of qufengtongluo recipe on expressions of HSPG mRNA and protein in Adriamycin-Induced Nephropathy rats
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2010Co-Authors: Wan-sen Sun, Yan-yun Ren, Yan-long ZhaoAbstract:OBJECTIVE To investigate the expression of HSPG in glomerular base membrane of Adriamycin-Induced Nephropathy (AN) rats, and the effect of Qufengtongluo recipe on HSPG mRNA expression and proteinuria in AN rats. METHODS One hundred forty rats were used in this study, including 32 rats in normal control group. AN was induced in the left rats by a single tail intravenous injection of adriamycin. Three weeks later, 90 AN rats were randomly divided into five groups; the Nephropathy group (B, n=18), the Qufeng group (C, n=18), Qufeng and prednisone group (D, n=18), prednisone group(E,n=18) and benazepri group (F, n= 18). The rats in these five groups were treated with different combination of Qufeng recipe and prednisone. In each group, renal tissue samples were collected at week 3 and 7. The distribution, expression of HSPG was examined by indirect immunofluorescence, and semi-quantity RT-PCR, respectively. RESULTS (1) In AN rats, the diffuse fusion and effacement of foot processes were observed when model established. (2) Compared with Nephropathy group, the average fluorescence intensity of HSPG dramatically increased in Qufeng group and prednisone group (P < 0.01), similarly, it also increased in D and F groups (P < 0.01). (3) Compared with Nephropathy group, the expression of HSPG mRNA was significantly up-regulated in other groups. (P < 0.01), especially in C and F groups. There was significant negative correlation between the expression of HSPG and quantity of 24-hour proteinuria. CONCLUSION The abnormal expression of HSPG and their altered distributions may be an important molecular mechanism that leads to the occurrence and development of proteinuria in AN rats. The effect of Qufengtongluo recipe on nephrotic syndrome might be related to the alteration of HSPG expression and distribution in glomerulus.
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Effect of Qufengtongluo recipe on expression of nephrin mRNA in Adriamycin-Induced Nephropathy in rats
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2009Co-Authors: Yan-yun Ren, Yan-long Zhao, Wan-sen Sun, Zhu WangAbstract:OBJECTIVE To investigate the expression of nephrin mRNA in Adriamycin-Induced Nephropathy (AN) in rats, and to explore the effect of Qufengtongluo recipe on proteinuria in AN rats and on the expression of nephrin mRNA. METHODS Adriamycin Nephropathy was induced by a single tail intravenous injection of adriamycin. We randomly divided 140 rats into a normal control group (n=32) and a Nephropathy model group (n=108). Three weeks later, 90 AN rats were randomly divided into 5 groups: a model group, a qufeng group, a qufeng and prednisone group, a prednisone group, and a benazepri group (18 rats in each group). They were treated respectively, and renal tissue samples were collected at week 0, 3, 5, and 7, respectively. The distribution and expression of nephrin mRNA were examined by indirect immunofluorescence and semi-quantity RT-PCR. RESULTS In the AN rats, the diffuse fusion and effacement of foot processes were observed at week 3. The fluorescence intensity of nephrin and the expression of nephrin mRNA significantly increased in the qufeng group and the prednisone group compared with the model group at the week 7 (P
Lin Qing - One of the best experts on this subject based on the ideXlab platform.
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Experimental Study of Shenkangling on Serum Proteomics of Adriamycin-Induced Nephrotic Rats
Journal of Fujian University of Traditional Chinese Medicine, 2011Co-Authors: Lin QingAbstract:Objective To screen the serum proteomic patterns of rats with Adriamycin-Induced Nephropathy by serum proteomics for discussion of the biomarker of frequent relapse nephrotic syndrome and target point of medication.Methods Surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS) and CM10 protein clips was used to detect the serum proteomic patterns of rats in the five groups,and proteins with differential expression of each group were analyzed by Ciphergen Proteinchip Software 3.2.1 and Biomarker Wizard software.Results Proteins with differential expression were detected in the mass charge ratio range of 2 000-50 000(P0.05),there were 25 proteins between group B and group A,5 proteins between group B and group C,3 proteins decreased progressively from the group B,to the group C,to the group D,group E,and to the group A.Conclusion The serum proteomic patterns of rats with Adriamycin-Induced Nephropathy can be analyzed effectively by SELDI-TOF-MS.The difference of proteomics was significant before and after intervention of Shenkangling.The curative effects of Shenkangling combined with prednisone are superior to those of prednisone alone for the treatment of Adriamycin-Induced nephrotic rats.
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The Empirical Study on Blood Proteomics of Rats with Adriamycin-Induced Nephropathy by Treament of Decoction Shenkangling
Chinese Journal of Integrated Traditional and Western Nephrology, 2010Co-Authors: Lin QingAbstract:Objective:Purpose To analyze the blood proteomic patterns of rats with Adriamycin-Induced Nephropathy by blood proteomics,for discussing the biomarker of Frequent Relapse Nephrotic Syndrome and target point of medication.Methods:Surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)CM10 protein clips was used to detect the blood proteomic patterns of every rat,PBS Ⅱ-C protein clips reading machinewas applied to collect data automaticly,proteins with differential expression between each group were analyzed by Ciphergen Proteinchip Software 3.2.1 and Biomarker Wizard software.Results:About 30 proteins with differential expression were detected in the mass charge ratio range of 2 000～50 000(P0.05),there were 25 proteins between B group and A group,5 proteins between B group and C group,3 proteins decreased progressively from B group→C group→D、E group→A group,they were 8 837.7、15 013.0、15 053.2.Conclusion:The blood proteomic patterns of rats with Adriamycin-Induced Nephropathy can be analyzed in effect by SELDI-TOF-MS,significantly changed by treament of decoction Shenkangling.
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A Urinary Proteomics Mass Spectromentry Detection and Chinese Intervention of Shenkangling
Chinese Journal of Integrated Traditional and Western Nephrology, 2010Co-Authors: Lin QingAbstract:Objective:To screening the urinary proteomic patterns of rats with Adriamycin-Induced Nephropathy by urinary proteomics for discussion of the biomarker of Frequent Relapse Nephrotic Syndrome and target point of medication.Methods:We copied Adriamycin-Induced Nephropathy (AN) rats model with adriamycin (ADR) 5.5 mg/kg.All rats were divided into 5 groups:the normal group(group A),the Adriamycin-Induced Nephropathy group (group B),the prednisone group (group C),the prednisone and the Shenkangling(light concentration) group (group D),the prednisone and Shenkangling (high concentration) group(group E).Surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) CM10 protein clips were used to detect the urinary proteomic patterns of every rat.PBS Ⅱ-C protein clips reading machine was applied to collect data automatically.Proteins with differential expression between each group were analyzed by Ciphergen Proteinchip Software 3.2.1 and Biomarker Wizard software. And changes were observed on urinary protein,serum albumin (Alb),cholesterol (TC),triglyceride (TG),blood urea nitrogen (BUN),serum creatinine (Scr) and renal pathology in each group before and after treatment.Results:About 40 proteins with differential expressions were detected in the mass charge ratio range of 2 000～50 000 (P0.05),there were 38 proteins between group B and group A,3 proteins decreased progressively from the B group,to the C group,the D group to the E group and to the A group.M/Z were 9 321.0、9 393.1、9 477.5.Conclusion:The urinary proteomic patterns of rats with Adriamycin-Induced Nephropathy can be analyzed effectively by SELDI-TOF-MS.The difference of proteomics was significant before and after intervention of decoction Shenkangling.Traditional Chinese medicine Shenkangling combined prednisone was more effectively than prednisone alone in the treatment of AN rats.
