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Thomas A. Horbett – One of the best experts on this subject based on the ideXlab platform.

  • Platelet adhesion to radiofrequency glow-discharge-deposited fluorocarbon polymers preAdsorbed with selectively depleted plasmas show the primary role of Fibrinogen.
    Journal of Biomaterials Science Polymer Edition, 2004
    Co-Authors: Wei-bor Tsai, J. M. Grunkemeier, Q. Shi, Clive D. Mcfarland, Thomas A. Horbett

    Abstract:

    Fluorocarbon radio-frequency glow-discharge (RFGD) treatment has previously been shown to cause decreased platelet adhesion despite the presence of Adsorbed Fibrinogen on the surfaces. In this study platelet adhesion to fluorocarbon RFGD-treated surfaces preAdsorbed with human plasma was further examined. A series of plasma deposited fluorocarbon thin films were made by varying the C3F6/CH4 ratio in the monomer feed. The surfaces were preAdsorbed with plasma, serum, or plasma selectively depleted of fibronectin, vitronectin, or Von Willebrand factor, and platelet adhesion was measured. We also measured Fibrinogen adsorption to the surfaces from plasma, monoclonal antibody binding to Adsorbed Fibrinogen and SDS elutability of the Adsorbed Fibrinogen. The antibodies used bind to the three putative platelet binding sites on Fibrinogen, namely, M1 antibody binds to the dodecapeptide at the C-terminus of the gamma chain, γ (402–411), R1 antibody binds to a sequence in the Aα chain (87–100) which includes RGDF …

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  • Variations in the ability of Adsorbed Fibrinogen to mediate platelet adhesion to polystyrene-based materials: A multivariate statistical analysis of antibody binding to the platelet binding sites of Fibrinogen
    Journal of biomedical materials research. Part A, 2003
    Co-Authors: Wei-bor Tsai, J. M. Grunkemeier, Thomas A. Horbett

    Abstract:

    Platelet adhesion to the surfaces of biomaterials preAdsorbed with plasma previously has been shown to be mediated exclusively by surface-bound Fibrinogen and does not seem to involve the other adhesion proteins in plasma (Tsai et al., J Biomed Mater Res 2002;60:348–359). In this study, the influence of surface-bound Fibrinogen on platelet adhesion to five different types of polystyrene-based microtiter plates preAdsorbed with plasma was analyzed relative to the amount of Adsorbed Fibrinogen and monoclonal antibody binding to the Adsorbed Fibrinogen. There was no significant correlation between platelet adhesion and the absolute amount of Adsorbed Fibrinogen. However, platelet adhesion was positively correlated to the ability of the Adsorbed Fibrinogen to bind three types of monoclonal antibodies. The antibodies used bound to the sites on Fibrinogen thought to be involved in platelet binding (the two γ chain C-terminal dodecapeptides and the RGDF and RGDS sequences in each of the Aα chains). A partial least-squares calibration model was used to analyze the relative importance of these binding sites in Fibrinogen to platelet adhesion. The γ chain C-terminal dodecapeptide was shown to be the most important site in Adsorbed Fibrinogen in mediating platelet adhesion. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1255–1268, 2003

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  • Characterization of the structure of binary and ternary Adsorbed protein films using electron spectroscopy for chemical analysis, time-of-flight secondary ion mass spectrometry, and radiolabeling
    Langmuir, 2003
    Co-Authors: Matthew Scott Wagner, Thomas A. Horbett, David G. Castner

    Abstract:

    In complex Adsorbed protein films, the biological reactivity of the Adsorbed proteins depends on their relative concentrations, organization, conformation, and orientation. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) samples the outermost composition of an Adsorbed protein film, providing biologically relevant information about the organization and availability of the Adsorbed proteins in the film. We have previously shown that ToF-SIMS can quantitatively measure the composition of binary Adsorbed protein films. However, for one particular binary system studied (immunoglobulin G−Fibrinogen), a difference between the ToF-SIMS and 125I-radiolabeled protein adsorption measurements was reported. It was hypothesized that the composition of the protein film at its outermost (and, therefore, most biologically relevant) surface was different than that of the overall protein film due to the protrusion of the Adsorbed Fibrinogen over the Adsorbed immunoglobulin G. This study provides further evidence …

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J. M. Grunkemeier – One of the best experts on this subject based on the ideXlab platform.

  • Platelet adhesion to radiofrequency glow-discharge-deposited fluorocarbon polymers preAdsorbed with selectively depleted plasmas show the primary role of Fibrinogen.
    Journal of Biomaterials Science Polymer Edition, 2004
    Co-Authors: Wei-bor Tsai, J. M. Grunkemeier, Q. Shi, Clive D. Mcfarland, Thomas A. Horbett

    Abstract:

    Fluorocarbon radio-frequency glow-discharge (RFGD) treatment has previously been shown to cause decreased platelet adhesion despite the presence of Adsorbed Fibrinogen on the surfaces. In this study platelet adhesion to fluorocarbon RFGD-treated surfaces preAdsorbed with human plasma was further examined. A series of plasma deposited fluorocarbon thin films were made by varying the C3F6/CH4 ratio in the monomer feed. The surfaces were preAdsorbed with plasma, serum, or plasma selectively depleted of fibronectin, vitronectin, or Von Willebrand factor, and platelet adhesion was measured. We also measured Fibrinogen adsorption to the surfaces from plasma, monoclonal antibody binding to Adsorbed Fibrinogen and SDS elutability of the Adsorbed Fibrinogen. The antibodies used bind to the three putative platelet binding sites on Fibrinogen, namely, M1 antibody binds to the dodecapeptide at the C-terminus of the gamma chain, γ (402–411), R1 antibody binds to a sequence in the Aα chain (87–100) which includes RGDF …

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  • Variations in the ability of Adsorbed Fibrinogen to mediate platelet adhesion to polystyrene-based materials: A multivariate statistical analysis of antibody binding to the platelet binding sites of Fibrinogen
    Journal of biomedical materials research. Part A, 2003
    Co-Authors: Wei-bor Tsai, J. M. Grunkemeier, Thomas A. Horbett

    Abstract:

    Platelet adhesion to the surfaces of biomaterials preAdsorbed with plasma previously has been shown to be mediated exclusively by surface-bound Fibrinogen and does not seem to involve the other adhesion proteins in plasma (Tsai et al., J Biomed Mater Res 2002;60:348–359). In this study, the influence of surface-bound Fibrinogen on platelet adhesion to five different types of polystyrene-based microtiter plates preAdsorbed with plasma was analyzed relative to the amount of Adsorbed Fibrinogen and monoclonal antibody binding to the Adsorbed Fibrinogen. There was no significant correlation between platelet adhesion and the absolute amount of Adsorbed Fibrinogen. However, platelet adhesion was positively correlated to the ability of the Adsorbed Fibrinogen to bind three types of monoclonal antibodies. The antibodies used bound to the sites on Fibrinogen thought to be involved in platelet binding (the two γ chain C-terminal dodecapeptides and the RGDF and RGDS sequences in each of the Aα chains). A partial least-squares calibration model was used to analyze the relative importance of these binding sites in Fibrinogen to platelet adhesion. The γ chain C-terminal dodecapeptide was shown to be the most important site in Adsorbed Fibrinogen in mediating platelet adhesion. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1255–1268, 2003

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  • Co-Adsorbed Fibrinogen and von Willebrand factor augment platelet procoagulant activity and spreading.
    Journal of Biomaterials Science Polymer Edition, 2001
    Co-Authors: J. M. Grunkemeier, Wei-bor Tsai, Thomas A. Horbett

    Abstract:

    Previously we observed that platelets adherent to surfaces preAdsorbed with blood plasma exhibited 1.3 to 2.4 times greater procoagulant activity than platelets on surfaces Adsorbed with Fibrinogen (Fg) only. These observations suggested that the adhesion proteins Adsorbed from plasma may activate platelets in a cooperative, or synergistic manner. In the present study, polystyrene surfaces Adsorbed with both Fg and vWF induced up to three times greater procoagulant activity than surfaces Adsorbed with Fg or vWF only. The amounts of Fg and vWF Adsorbed from binary mixtures that resulted in increased procoagulant activity were found to be similar to the amounts that Adsorbed to PS from 100% plasma. The effect of Adsorbed adhesion proteins on platelet spreading was also investigated. The proportion of fully spread platelets increased, depending on the adhesion protein preAdsorbed to the surface, in the following order: vWF < Fg < Fn < (vWF + Fg) < Vn < plasma.

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Nan Huang – One of the best experts on this subject based on the ideXlab platform.

  • mussel inspired one step adherent coating rich in amine groups for covalent immobilization of heparin hemocompatibility growth behaviors of vascular cells and tissue response
    ACS Applied Materials & Interfaces, 2014
    Co-Authors: Ying Yang, Manfred F. Maitz, Pengkai Qi, Xiangyang Li, Zhilu Yang, Ru Shen, Qiufen Tu, Nan Huang

    Abstract:

    Heparin, an important polysaccharide, has been widely used for coatings of cardiovascular devices because of its multiple biological functions including anticoagulation and inhibition of intimal hyperplasia. In this study, surface heparinization of a commonly used 316L stainless steel (SS) was explored for preparation of a multifunctional vascular stent. Dip-coating of the stents in an aqueous solution of dopamine and hexamethylendiamine (HD) (PDAM/HD) was presented as a facile method to form an adhesive coating rich in primary amine groups, which was used for covalent heparin immobilization via active ester chemistry. A heparin grafting density of about 900 ng/cm2 was achieved with this method. The retained bioactivity of the immobilized heparin was confirmed by a remarkable prolongation of the activated partial thromboplastin time (APTT) for about 15 s, suppression of platelet adhesion, and prevention of the denaturation of Adsorbed Fibrinogen. The Hep-PDAM/HD also presented a favorable microenvironment…

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  • Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation
    Chinese Journal of Chemical Physics, 2014
    Co-Authors: Ansha Zhao, Manfred F. Maitz, Zhao Wang, Xiao-hua Zhu, Nan Huang

    Abstract:

    The adsorption of Fibrinogen can be used as a quick indicator of surface haemocompatibility because of its prominent role in coagulation and platelet adhesion. In this work the molecular interaction between Fibrinogen and a modified titanium oxide surface/platelet has been studied by quartz crystal microbalance with dissipation (QCM-D) in situ. In order to further characterize the conformation of Adsorbed Fibrinogen, αC and γ-chain antibody were used to check the orientation and denaturation of Fibrinogen on solid surface. QCM-D investigations revealed the Fibrinogen have the trend to adsorb on hydrophilic surface in a side-on orientation by positively charged αC domains, which would reduce the exposure of platelet bonding site on γ chain and enable less platelet adhesion and be activated. These observations suggest that certain conformations of Adsorbed Fibrinogen are less platelet adhesive than others, which opens a possibility for creating a non-platelet adhesive substrates.

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