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Djurdjina Ružic – One of the best experts on this subject based on the ideXlab platform.

  • ploidy level stability of Adventitious Shoots of sour cherry cacanski rubin and gisela 5 cherry rootstock
    Plant Cell Tissue and Organ Culture, 2012
    Co-Authors: Tatjana Vujovic, Radosav Cerovic, Djurdjina Ružic
    Abstract:

    The capacity of regeneration of Adventitious Shoots from leaf explants was studied in sour chercherry ‘Cacanski Rubin’ (Prunus cerasus L.) and cherry rootstock Gisela 5 (P. cerasus × P. canescens). Regeneration assay included thirty different combinations of plant growth regulators. 6-benzyladenine (BA) and thidiazuron (TDZ) were applied either individually or each combined with different concentrations of indole-3-butyric acid, α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). ‘Cacanski Rubin’ showed higher regeneration capacity in comparison with Gisela 5 regarding the total number of treatments inducing regeneration as well as the highest frequency of regeneration achieved. In both genotypes, 8.9 μM BA was more effective than both 4.5 and 9.0 μM TDZ in inducing Adventitious regeneration, but only when combined with auxins. The highest frequency of regeneration (20.8 %) in ‘Cacanski Rubin’ was achieved on medium supplemented with 8.9 μM BA combined with 5.4 μM NAA, while in Gisela 5 the highest value (8.3 %) was obtained when BA was combined with 4.5 μM 2,4-D. Flow cytometry combined with 4′-6-diamidino-2-phenylindole staining was employed to estimate DNA ploidy levels and relative nuclear DNA content in Adventitious regeneration-derived Shoots, in vitro Shoots of axillary origin and in vivo control plants from open field. No significant differences in nuclear DNA content were detected among plants of different origin. Chromosome counting in root tip meristems also showed normal tetraploid chromosome number (2n = 4x = 32) in ‘Cacanski Rubin’ Shoots and normal triploid chromosome number (2n = 3x = 24) in Gisela 5 Shoots regenerated in vitro. The results obtained suggest that no major genetic instability occurred during Adventitious regeneration under the described experimental conditions.

Djurdjina Ružić – One of the best experts on this subject based on the ideXlab platform.

  • Ploidy level stability of Adventitious Shoots of sour cherry ‘Čačanski Rubin’ and Gisela 5 cherry rootstock
    Plant Cell Tissue and Organ Culture (PCTOC), 2012
    Co-Authors: Tatjana Vujović, Radosav Cerović, Djurdjina Ružić
    Abstract:

    The capacity of regeneration of Adventitious Shoots from leaf explants was studied in sour chercherry ‘Čačanski Rubin’ ( Prunus cerasus L.) and cherry rootstock Gisela 5 ( P. cerasus  ×  P. canescens ). Regeneration assay included thirty different combinations of plant growth regulators. 6-benzyladenine (BA) and thidiazuron (TDZ) were applied either individually or each combined with different concentrations of indole-3-butyric acid, α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). ‘Čačanski Rubin’ showed higher regeneration capacity in comparison with Gisela 5 regarding the total number of treatments inducing regeneration as well as the highest frequency of regeneration achieved. In both genotypes, 8.9 μM BA was more effective than both 4.5 and 9.0 μM TDZ in inducing Adventitious regeneration, but only when combined with auxins. The highest frequency of regeneration (20.8 %) in ‘Čačanski Rubin’ was achieved on medium supplemented with 8.9 μM BA combined with 5.4 μM NAA, while in Gisela 5 the highest value (8.3 %) was obtained when BA was combined with 4.5 μM 2,4-D. Flow cytometry combined with 4′-6-diamidino-2-phenylindole staining was employed to estimate DNA ploidy levels and relative nuclear DNA content in Adventitious regeneration-derived Shoots, in vitro Shoots of axillary origin and in vivo control plants from open field. No significant differences in nuclear DNA content were detected among plants of different origin. Chromosome counting in root tip meristems also showed normal tetraploid chromosome number (2 n  = 4 x  = 32) in ‘Čačanski Rubin’ Shoots and normal triploid chromosome number (2 n  = 3 x  = 24) in Gisela 5 Shoots regenerated in vitro. The results obtained suggest that no major genetic instability occurred during Adventitious regeneration under the described experimental conditions.

Jaime Teixeira A Da Silva – One of the best experts on this subject based on the ideXlab platform.

  • somatic embryogenesis and shoot organogenesis from leaf explants of primulina tabacum
    Plant Cell Tissue and Organ Culture, 2012
    Co-Authors: Xingyu Yang, Jaime Teixeira A Da Silva
    Abstract:

    Primulina tabacum is a rare and endangered species that is endemic to China. Establishing an efficient regeneration system is necessary for its conservation and reintroduction. In this study, when leaf explants collected from plants grown in four ecotypes in China are incubated on Murashige and Skoog (MS) medium containing 5.0 μM thidiazuron (TDZ) for 30 days, then transferred to medium containing 5.0 μM 6-benzyladenine (BA), Adventitious Shoots are then observed. Conversely, when leaf explants are incubated on medium containing 5.0 μM BA for 30 days, then transferred to medium containing 5.0 μM TDZ, somatic embryogenesis is induced. This indicates that somatic embryogenesis and shoot organogenesis could be switched simply by changing the order of two cytokinins supplemented in the culture medium. Histological investigation has revealed that embryogenic cells are induced within 30 days following incubation of explants in medium containing TDZ. Only if embryogenic cells were induced, TDZ could enhance somatic embryogenesis and BA could stimulate shoot organogenesis. When comparing explants from different ecotypes, leaf explants from Zixiadong in Hunan Province could induce low numbers (1–2) of either somatic embryos or Adventitious Shoots on medium containing either 5.0 μM TDZ or 5.0 μM BA, respectively. Whereas, leaf explants from plants collected from the other three ecological habitats could induce 50–70 somatic embryos/Adventitious Shoots per explant. Moreover, somatic embryos could induce secondary somatic embryogenesis and Adventitious Shoots on different media. All regenerated Shoots developed Adventitious roots when these are transferred to rooting medium, and over 95% of plantlets have survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite).

  • vegetative propagation of primulina tabucum hance by petiole cuttings
    Scientia Horticulturae, 2012
    Co-Authors: Jaime Teixeira A Da Silva
    Abstract:

    Abstract Primulina tabucum Hance is a first grade rare and endangered monospecific genus endemic to China. Petioles derived from one-year old potted plants (originally derived from in vitro culture) were used to study the effect of substrate and plant growth regulators on the induction of Adventitious Shoots and roots. Sand and perlite (1:1) was the most suitable substrate. Petiole explants dipped in 4.9 μM indole-3-butyric acid for 30 min could induce the highest rooting percentage (90%) and an average of 8.8 Adventitious Shoots per petiole, establishing thus a simple, quick and efficient propagation pathway. This simple protocol would allow for mass production of ex vivo– or in vitro-derived germplasm conservation.

  • shoot organogenesis and plant regeneration in metabriggsia ovalifolia
    Plant Cell Tissue and Organ Culture, 2011
    Co-Authors: Jaime Teixeira A Da Silva, Xinhua Zhang, Jietang Zhao
    Abstract:

    An efficient propagation and regeneration system via direct shoot organogenesis for an endangered species, Metabriggsia ovalifolia, was established. High activity cytokinins [6-benzyladeneine (BA) and thidiazuron (TDZ)] and low activity auxins [α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA)] could directly induce Adventitious Shoots from leaf or petiole explants within 5 weeks. Cytokinins (TDZ or BA) combined with auxin (NAA) in the induction media induced more Adventitious Shoots than when auxins or cytokinins were used alone. Adventitious Shoots could be induced and also mass-propagated on media containing 2.5–5.0 μM TDZ (or BA) and 0.25–0.5 μM NAA. Adventitious roots differentiated at the proximal end of Shoots on rooting media containing half-strength MS salts and 0.5 μM IBA, 0.5 μM NAA, 0.1% activated charcoal or no plant growth regulators. Over 90% of plantlets survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite) in basins.

K. Miyoshi – One of the best experts on this subject based on the ideXlab platform.

  • The efficient induction of tetraploid plants via Adventitious Shoots in Antirrhinum majus L. by in vitro seed treatment with amiprophos-methyl
    Plant Cell Tissue and Organ Culture (PCTOC), 2020
    Co-Authors: The Su Hlaing, H. Kondo, A. Deguchi, K. Miyoshi
    Abstract:

    APM induced Adventitious Shoots on the hypocotyl of seedling by seed treatment and the induced-Adventitious Shoots were identified to be tetraploids. Osmopriming accelerated the frequency of tetraploid induction. Tetraploid plants of Antirrhinum majus L. were obtained from the seeds of the F_1 variety ‘Maryland True Pink’ by amiprophos-methyl (APM) treatment and subsequent in vitro culture. Seeds were treated with 0, 10 and 20 mgl^−1 APM for 1, 3, or 7 d (day) with or without osmopriming. Overall, the seedlings established from the seeds treated with 10 or 20 mgl^−1 APM for 3–7 d exhibited malformation with rudimentary roots and swollen hypocotyls that eventually produced Adventitious Shoots. The highest Adventitious shoot formation frequency reached 71.6% in non-primed seeds treated with 10 mgl^−1 APM for 7 d; this decreased to 54.4% in osmoprimed seeds that received the same APM treatment. The plantlets from the Adventitious Shoots were identified as tetraploids at frequencies of 51.4% and 90.4%, respectively. Tetraploid frequency increased with higher concentration and/or longer duration of APM treatment and ranged from 0 to 65.9% in the non-primed seeds and 10.3% to 100% in the osmoprimed seeds. Osmopriming of seeds accelerated the induction of tetraploids by APM treatment at the same concentration and duration by 10.3–39.0%, except in the case of treatment with 20 mgl^−1 APM for 3 d treatment. In the osmoprimed seeds treated with 20 mgl^−1 APM for 7 d, all the plantlets from the Adventitious Shoots were revealed to be tetraploids. This study revealed that APM has dual effects, namely the inhibitory effect on the growth of seedlings and the stimulatory effect on the induction of Adventitious Shoots on the hypocotyl of seedlings.

  • The efficient induction of tetraploid plants via Adventitious Shoots in Antirrhinum majus L. by in vitro seed treatment with amiprophos-methyl
    Plant Cell Tissue and Organ Culture (PCTOC), 2020
    Co-Authors: H. Kondo, A. Deguchi, K. Miyoshi
    Abstract:

    Tetraploid plants of Antirrhinum majus L. were obtained from the seeds of the F1 variety ‘Maryland True Pink’ by amiprophos-methyl (APM) treatment and subsequent in vitro culture. Seeds were treated with 0, 10 and 20 mgl−1 APM for 1, 3, or 7 d (day) with or without osmopriming. Overall, the seedlings established from the seeds treated with 10 or 20 mgl−1 APM for 3–7 d exhibited malformation with rudimentary roots and swollen hypocotyls that eventually produced Adventitious Shoots. The highest Adventitious shoot formation frequency reached 71.6% in non-primed seeds treated with 10 mgl−1 APM for 7 d; this decreased to 54.4% in osmoprimed seeds that received the same APM treatment. The plantlets from the Adventitious Shoots were identified as tetraploids at frequencies of 51.4% and 90.4%, respectively. Tetraploid frequency increased with higher concentration and/or longer duration of APM treatment and ranged from 0 to 65.9% in the non-primed seeds and 10.3% to 100% in the osmoprimed seeds. Osmopriming of seeds accelerated the induction of tetraploids by APM treatment at the same concentration and duration by 10.3–39.0%, except in the case of treatment with 20 mgl−1 APM for 3 d treatment. In the osmoprimed seeds treated with 20 mgl−1 APM for 7 d, all the plantlets from the Adventitious Shoots were revealed to be tetraploids. This study revealed that APM has dual effects, namely the inhibitory effect on the growth of seedlings and the stimulatory effect on the induction of Adventitious Shoots on the hypocotyl of seedlings. APM induced Adventitious Shoots on the hypocotyl of seedling by seed treatment and the induced-Adventitious Shoots were identified to be tetraploids. Osmopriming accelerated the frequency of tetraploid induction.

Xinhua Zhang – One of the best experts on this subject based on the ideXlab platform.

  • Shoot organogenesis and somatic embryogenesis from leaf and root explants of Scaevola sericea.
    Scientific reports, 2020
    Co-Authors: Hanzhi Liang, Xinhua Zhang, Yuping Xiong, Beiyi Guo, Haifeng Yan, Shuguang Jian, Hai Ren, Songjun Zeng
    Abstract:

    An efficient regeneration system via shoot organogenesis and somatic embryogenesis from in vitro leaf and root explants was established for Scaevola sericea for the first time. The highest axillary shoot proliferation coefficient (4.8) was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzyladenine (BA) and 0.1 mg/L α-naphthaleneacetic acid (NAA) every 45 days. Young in vitro leaves and roots, which were used as explants, were cultured onto medium supplemented with different plant growth regulators. Our results showed that only cytokinins BA and thidiazuron (TDZ), could induce Adventitious Shoots and somatic embryos from leaf and root explants. The optimal medium to achieve this was MS medium supplemented with 2.5 mg/L BA and which induced most Adventitious Shoots (2.7) and somatic embryos (17.3) from leaf explants within 30 days. From root explants, 1.1 Adventitious Shoots and 7.6 somatic embryos could be induced on MS medium supplemented with 2.5 mg/L TDZ. Histological observation showed that both somatic embryos and Adventitious Shoots were originated from homogeneous parenchyma and the development of somatic embryos was visible. Maximum rooting percentage (99.0%) was achieved on half-strength MS medium supplemented with 2.5 mg/L NAA. Well-rooted plantlets, which were transplanted into a substrate of pure river sand, displayed a high survival percentage of 91.7% after transplanting for 45 days while the best substrate for plantlet growth was river sand: coral sand (1:1).

  • Adventitious Shoot Induction from Internode and Root Explants in a Semiparasitic Herb Monochasma savatieri Franch ex Maxim
    Journal of Plant Growth Regulation, 2017
    Co-Authors: Yueya Zhang, Yulu Chen, Xinhua Zhang, Jaime A. Teixeira Da Silva, Guohua Ma
    Abstract:

    A highly efficient protocol for the induction of Adventitious Shoots from young internode and root explants of a semiparasitic medicinal herb Monochasma savatieri Franch ex Maxim was developed. MS basal medium supplemented with 5 µM thidiazuron (TDZ) induced 32 Adventitious Shoots/explant, which was double the number obtained using the same concentration of 6-benzyladenine (BA). Hyperhydric Shoots were observed when 10 µM of any cytokinin was added to MS media. Use of any cytokinin at 2.5 µM produced an average of 14–21 Adventitious Shoots/root explant. Shoots formed roots in vitro more effectively than α-naphthaleneacetic acid when indole-3-butyric acid and indole-3-acetic acid were used at 1.0 µM. Two-month-old rooted plantlets were transplanted to vermiculite and 70% survived after 4 months.

  • shoot organogenesis and plant regeneration in metabriggsia ovalifolia
    Plant Cell Tissue and Organ Culture, 2011
    Co-Authors: Jaime Teixeira A Da Silva, Xinhua Zhang, Jietang Zhao
    Abstract:

    An efficient propagation and regeneration system via direct shoot organogenesis for an endangered species, Metabriggsia ovalifolia, was established. High activity cytokinins [6-benzyladeneine (BA) and thidiazuron (TDZ)] and low activity auxins [α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA)] could directly induce Adventitious Shoots from leaf or petiole explants within 5 weeks. Cytokinins (TDZ or BA) combined with auxin (NAA) in the induction media induced more Adventitious Shoots than when auxins or cytokinins were used alone. Adventitious Shoots could be induced and also mass-propagated on media containing 2.5–5.0 μM TDZ (or BA) and 0.25–0.5 μM NAA. Adventitious roots differentiated at the proximal end of Shoots on rooting media containing half-strength MS salts and 0.5 μM IBA, 0.5 μM NAA, 0.1% activated charcoal or no plant growth regulators. Over 90% of plantlets survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite) in basins.