The Experts below are selected from a list of 114 Experts worldwide ranked by ideXlab platform

Jose Luis Martinez Vidal - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg(-1), and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg(-1), and Aflatoxin G2 at 50 μg kg(-1). Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 5 μg kg(-1) (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 10 μg kg(-1) (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    Abstract A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg−1, and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg−1, and Aflatoxin G2 at 50 μg kg−1. Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg−1 (for Aflatoxins B1, B2 and G1) to 5 μg kg−1 (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg−1 (for Aflatoxins B1, B2 and G1) to 10 μg kg−1 (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

Antonia Garrido Frenich - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg(-1), and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg(-1), and Aflatoxin G2 at 50 μg kg(-1). Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 5 μg kg(-1) (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 10 μg kg(-1) (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    Abstract A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg−1, and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg−1, and Aflatoxin G2 at 50 μg kg−1. Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg−1 (for Aflatoxins B1, B2 and G1) to 5 μg kg−1 (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg−1 (for Aflatoxins B1, B2 and G1) to 10 μg kg−1 (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

Maria Luz Gomezperez - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg(-1), and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg(-1), and Aflatoxin G2 at 50 μg kg(-1). Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 5 μg kg(-1) (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 10 μg kg(-1) (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    Abstract A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg−1, and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg−1, and Aflatoxin G2 at 50 μg kg−1. Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg−1 (for Aflatoxins B1, B2 and G1) to 5 μg kg−1 (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg−1 (for Aflatoxins B1, B2 and G1) to 10 μg kg−1 (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

Roberto Romerogonzalez - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg(-1), and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg(-1), and Aflatoxin G2 at 50 μg kg(-1). Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 5 μg kg(-1) (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg(-1) (for Aflatoxins B1, B2 and G1) to 10 μg kg(-1) (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

  • multi mycotoxin analysis in eggs using a quechers based extraction procedure and ultra high pressure liquid chromatography coupled to triple quadrupole mass spectrometry
    Journal of Chromatography A, 2011
    Co-Authors: Antonia Garrido Frenich, Roberto Romerogonzalez, Maria Luz Gomezperez, Jose Luis Martinez Vidal
    Abstract:

    Abstract A reliable and rapid method has been developed for the determination of 10 mycotoxins (beauvericin, enniatin A, A1, B1, citrinin, Aflatoxin B1, B2, G1, G2 and ochratoxin A) in eggs at trace levels. Ultra-high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) has been used for the analysis of these compounds in less than 7 min. Mycotoxins have been extracted from egg samples using a QuEChERS-based extraction procedure (Quick, Easy, Cheap, Effective, Rugged and Safe) without applying any further clean-up step. Extraction, chromatographic and detection conditions were optimised in order to increase sample throughput and sensitivity. Matrix-matched calibration was used for quantification. Blank samples were fortified at 10, 25, 50 and 100 μg kg−1, and recoveries ranged from 70% to 110%, except for ochratoxin A and Aflatoxin G1 at 10 μg kg−1, and Aflatoxin G2 at 50 μg kg−1. Relative standard deviations were lower than 25% in all the cases. Limits of detection ranged from 0.5 μg kg−1 (for Aflatoxins B1, B2 and G1) to 5 μg kg−1 (for enniatin A, citrinin and ochratoxin A) and limits of quantification ranged from 1 μg kg−1 (for Aflatoxins B1, B2 and G1) to 10 μg kg−1 (for enniatin A, citrinin and ochratoxin A). Seven samples were analyzed and Aflatoxins B1, B2, G1, G2, and beauvericin were detected at trace levels.

C Van Peteghem - One of the best experts on this subject based on the ideXlab platform.

  • development of a liquid chromatography tandem mass spectrometry method for the simultaneous determination of 16 mycotoxins on cellulose filters and in fungal cultures
    Rapid Communications in Mass Spectrometry, 2006
    Co-Authors: Barbara Delmulle, S De Saeger, An Adams, N De Kimpe, C Van Peteghem
    Abstract:

    The development of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of 16 mycotoxins possibly related to the ‘Sick Building Syndrome’ on filters and in fungal cultures is described. Fungi-surface sampling as regards the ‘Sick Building Syndrome’ preferably happens by scraping off fungal material and vacuuming onto cellulose filters. Therefore, these two media were used as samples. They were spiked with nivalenol, deoxynivalenol, zearalenone, diacetoxyscirpenol, T-2 toxin, verrucarol, verrucarin A, neosolaniol, sterigmatocystin, roridin A, ochratoxin A, Aflatoxin B1, Aflatoxin B2, Aflatoxin G1 and Aflatoxin G2, which can be produced by isolates from fungi-damaged buildings. Deepoxy-deoxynivalenol was used as internal standard. Samples were extracted with organic solvents and the different mycotoxins were separated by high-performance liquid chromatography (HPLC) using a C18 reversed-phase SunFire analytical column and a mobile phase of variable mixtures of ammonium acetate (10 mM) and sodium acetate (20 µM) in water (solvent A) and in methanol (solvent B). The samples were run on-line with a Micromass Quattro Micro triple quadrupole mass spectrometer in positive electrospray ionisation mode using multiple reaction monitoring (MRM). The detection limits of the procedure varied from 50 to 0.009 pg/µL for filter samples and from 75 to 0.04 pg/µL for fungal culture samples. As the method includes few and non-labourious sample treatment steps, it should allow for a high throughput of samples. Copyright © 2006 John Wiley & Sons, Ltd.