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Johannes A. Jehle – One of the best experts on this subject based on the ideXlab platform.

  • Agrotis segetum nucleopolyhedrovirus but not Agrotis segetum granulovirus replicate in AiE1611T cell line of Agrotisipsilon.
    Journal of invertebrate pathology, 2017
    Co-Authors: Gianpiero Gueli Alletti, Eric B. Carstens, Birgit Weihrauch, Johannes A. Jehle
    Abstract:

    Abstract Both Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV) belong to a cluster of four baculoviruses that are infective for different Agrotis species. Belonging further to different baculovirus genera, namely Alphabaculovirus and Betabaculovirus, respectively, AgseNPV-B and AgseGV are candidates to investigate virus interactions in co-infections. However, for the investigation of virus interactions on a cellular level, permissive insect cell-lines are needed. The cell line AiE1611T deriving from Agrotis ipsilon eggs has been shown to be permissive for several Alphabaculovirus isolates. In this study, virus replication was followed based on microscopic analysis of infected and transfected cells, as well as on a molecular level by PCR of DNA and cDNA of selected baculovirus transcripts. While the permissivity was not verified for AgseGV, AgseNPV-B produced occlusion bodies in both infection with hemolymph of infected larvae and Lipofectamin transfection with AgseNPV-B genomic DNA. In addition to the possibility to investigate virus interaction of AgseNPV-B with other alphabaculoviruses, the permissivity of AiE1611T for AgseNPV-B further offers the possibility a biological selection to separate AgseNPV-B from AgseGV.

  • The genome sequence of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) reveals a new baculovirus species within the Agrotis baculovirus complex
    Virus Genes, 2015
    Co-Authors: Jörg T. Wennmann, Gianpiero Gueli Alletti, Johannes A. Jehle
    Abstract:

    The genome of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) was completely sequenced and compared with whole genome sequences of the Agrotis segetum nucleopolyhedrovirus A (AgseNPV-A) and Agrotis ipsilon nucleopolyhedrovirus (AgipNPV). The AgseNPV-B genome is 148,981 bp in length and encodes 150 putative open reading frames. AgseNPV-B contains two copies of the gene viral enhancing factor ( vef ), making the Agrotis nucleopolyhedroviruses and A. segetum granulovirus (AgseGV) very rich in vef in comparison to other baculoviruses. Genome alignments of AgseNPV-B, AgseNPV-A and AgipNPV showed a very high genome co-linearity interspersed with variable regions, which are considered as putative sites of genomic recombination. Phylogenetic analyses revealed that all three viruses are distinct. However, AgseNPV-B is more closely related to AgipNPV suggesting that both viruses are at an early stage of phylogenetic divergence. It is proposed that AgseNPV-B belongs to a third Alphabaculovirus species of the Agrotis baculovirus complex. The Agrotis exclamationis nucleopolyhedrovirus (AgexNPV) shared high nucleotide sequence identities with AgseNPV-B, suggesting it is actually an AgseNPV-B isolate.

  • Mortality of cutworm larvae is not enhanced by Agrotis segetum granulovirus and Agrotis segetum nucleopolyhedrovirus B coinfection relative to single infection by either virus.
    Applied and environmental microbiology, 2015
    Co-Authors: Jörg T. Wennmann, Gianpiero Gueli Alletti, Tim Köhler, Johannes A. Jehle
    Abstract:

    Mixed infections of insect larvae with different baculoviruses are occasionally found. They are of interest from an evolutionary as well as from a practical point of view when baculoviruses are applied as biocontrol agents. Here, we report mixed-infection studies of neonate larvae of the common cutworm, Agrotis segetum, with two baculoviruses, Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV). By applying quantitative PCR (qPCR) analysis, coinfections of individual larvae were demonstrated, and occlusion body (OB) production within singly infected and coinfected larvae was determined in individual larvae. Mixtures of viruses did not lead to changes in mortality rates compared with rates of single-virus treatments, indicating an independent action within host larvae under our experimental conditions. AgseNPV-B-infected larvae showed an increase in OB production during 2 weeks of infection, whereas the number of AgseGV OBs did not change from the first week to the second week. Fewer OBs of both viruses were produced in coinfections than in singly infected larvae, suggesting a competition of the two viruses for larval resources. Hence, no functional or economic advantage could be inferred from larval mortality and OB production from mixed infections of A. segetum larvae with AgseNPV-B and AgseGV.

Germán San Blas – One of the best experts on this subject based on the ideXlab platform.

  • A morphological phylogeny of Agrotis Ochsenheimer (Lepidoptera, Noctuidae), with emphasis on the South American species
    Zoologica Scripta, 2014
    Co-Authors: Germán San Blas
    Abstract:

    The monophyly of Agrotis Ochsenheimer in addition to the relationships among the South American species of the genus and the relationship between Agrotis and Feltia Walker are examined. A cladistic analysis was performed based on 45 terminals, with 28 ingroup and 17 outgroup taxa, and 126 characters (two continuous and 124 discrete) from adult morphology, including male and female genitalia. Parsimony analyses were performed under equal and implied weighting. Results support Agrotis as a monophyletic group, sister to the South American species of Feltia (Feltia tent.), and the latter being more closely related to Agrotis than to the ‘true’ Feltia. Species of Agrotis that branched off first (including the type species) have characters shared with both Feltia and Agrotis. South American species of Agrotis (with some proviso) form a clade that branched off later, with some weakly supported species groups that had been proposed in previous works. Biogeographical aspects of the group are discussed after optimizing species distributions on the tree. Trees obtained after analyses using implied weights show similar relationships to those under equal weights, particularly regarding Feltia tent., showing Feltia tent. plus Agrotis forming a monophyletic group, sister to the ‘true’ Feltia.

  • Agrotis Ochsenheimer (Lepidoptera, Noctuidae): a systematic analysis of South American species.
    Zootaxa, 2014
    Co-Authors: Germán San Blas
    Abstract:

    The genus Agrotis Ochsenheimer, 1816 (Lepidoptera, Noctuidae) contains about 300 described species distributed worldwide, excepting the Poles. For South America 93 species have been described. Different diagnostic characters have been proposed for species from the northern Hemisphere, mostly from male genitalia. Recently, numerous South American species of the genus have been transferred to other genera. In this work, a systematic revision was undertaken of the South American species of Agrotis , restricting to 20 the number of species of this genus for the region and transferring the other species to different genera and/or synonymizing with other species. Based on a detailed study of the external morphology and genitalia of both sexes, several nomen clatural changes are proposed. New generic synonymy: Mesembreuxoa Hampson = Feltia Walker. New Agrotis synonymies include: Scotia forsteri Kohler = A. propriens (Dyar); Agrotis peruviana hampsoni Draudt, RhizAgrotis triclava Draudt, and Euxoa andina Kohler = A. peruviana (Hampson); Lycophotia achromatica Hampson, Feltia malefida patagiata Aurivillius, Prout and Meyrick, Agrotis psammophila Kohler, and Scotia ( Feltia ) canietensis Kohler = A. malefida Guenee; ChorizAgrotis benefida Draudt = A. experta (Walker); Agrotis livens Kohler and Agrotis capayana Kohler = A. araucaria (Hampson). Species transferred to Feltia Walker tent. include: Scotia aspersula Kohler, n. comb.; PorosAgrotis brachystria Hampson, n. comb.; Agrotis carrascoi Kohler, n. comb.; Mesembreuxoa chilensis Hampson, n. comb.; Euxoa clavisigna Dognin, n. comb.; Euxoa conifrons Draudt, n. comb.; Agrotis consternans Hayes, n. comb.; Euxoa coquimbensis Hampson, n. comb.; Mesembreuxoa fasicola Dyar, n. comb.; ChorizAgrotis forasmicans Kohler, n. comb.; Agrotis giselae Leon, n. comb.; Agrotis gypaetina Guenee, n. comb.; Agrotis hispidula Guenee, n. comb.; Euxoa incarum Cockerell, n. comb.; Agrotis india Kohler, n. comb.; Scotia mansa Kohler, n. comb.; Scotia picata Kohler, n. comb.; Agrotis rondanelli Leon, n. comb.; Euxoa senta Draudt, n. comb.; and Agrotis submontana Kohler, n. comb. New Feltia tent. synonymies include: Agrotis daguerrei Kohler, PorosAgrotis atricentrica Hampson, and Agrotis llanoi Kohler = F. brachystria ; Lycophotia baeckstroemi Aurivillius = F. deprivata (Walker); Agrotis raveni Kohler = F. fasicola ; Agrotis gentilii Kohler = F. forasmicans ; Scotia nyei Kohler, Euxoa australis Kohler, and Scotia liniclinans Kohler = F. hispidula ; Euxoa bosqui Kohler and Euxoa griseosparsa Kohler = F. lutescens (Blanchard); Euxoa praeocupata Kohler, Agrotis andinicola Kohler, and Scotia songoensis Kohler = F. subandina (Kohler); Agrotis maldonadoi Kohler = F. submontana . New combinations of Agrotis species: Anicla albiorbis (Dyar), n. comb.; Noctubourgognea chimaera (Kohler), n. comb.; Noctubourgognea dissociata (Staudinger), n. comb.; Pseudoleucania nigrocollaris (Kohler), n. comb.; and P. wittmeri (Kohler), n. comb. One lectotype and one paralectotype are designated for A. edmondsi Butler. The 20 species of South American Agrotis are redescribed using characters of color pattern, external morphology of head and thorax, and internal morphology of male (including vesica) and female genitalia. This is the first time complete eversions and description of male genitalia (aedeagus and vesica) and female genitalia are done for almost all the South American species. Images of all described characters and dichotomous keys to identify South American species of Agrotis are included.

  • Figures 94 – 99 In Agrotis Ochsenheimer (Lepidoptera, Noctuidae): A Systematic Analysis Of South American Species
    , 2014
    Co-Authors: Germán San Blas
    Abstract:

    FIGURES 94 – 99. Distribution maps of Agrotis species, edmondsi – group. 94, A. steniptera. 95, A. propriens. 96, A. bistrigata. 97, A. dispar. 98, A. peruviana. 99, A. elegans.

Gianpiero Gueli Alletti – One of the best experts on this subject based on the ideXlab platform.

  • Agrotis segetum nucleopolyhedrovirus but not Agrotis segetum granulovirus replicate in AiE1611T cell line of Agrotisipsilon.
    Journal of invertebrate pathology, 2017
    Co-Authors: Gianpiero Gueli Alletti, Eric B. Carstens, Birgit Weihrauch, Johannes A. Jehle
    Abstract:

    Abstract Both Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV) belong to a cluster of four baculoviruses that are infective for different Agrotis species. Belonging further to different baculovirus genera, namely Alphabaculovirus and Betabaculovirus, respectively, AgseNPV-B and AgseGV are candidates to investigate virus interactions in co-infections. However, for the investigation of virus interactions on a cellular level, permissive insect cell-lines are needed. The cell line AiE1611T deriving from Agrotis ipsilon eggs has been shown to be permissive for several Alphabaculovirus isolates. In this study, virus replication was followed based on microscopic analysis of infected and transfected cells, as well as on a molecular level by PCR of DNA and cDNA of selected baculovirus transcripts. While the permissivity was not verified for AgseGV, AgseNPV-B produced occlusion bodies in both infection with hemolymph of infected larvae and Lipofectamin transfection with AgseNPV-B genomic DNA. In addition to the possibility to investigate virus interaction of AgseNPV-B with other alphabaculoviruses, the permissivity of AiE1611T for AgseNPV-B further offers the possibility a biological selection to separate AgseNPV-B from AgseGV.

  • Mortality of cutworm larvae is not enhanced by Agrotis segetum granulovirus and Agrotis segetum nucleopolyhedrovirus B coinfection relative to single infection by either virus.
    Applied and environmental microbiology, 2015
    Co-Authors: Jörg T. Wennmann, Gianpiero Gueli Alletti, Tim Köhler, Johannes A. Jehle
    Abstract:

    Mixed infections of insect larvae with different baculoviruses are occasionally found. They are of interest from an evolutionary as well as from a practical point of view when baculoviruses are applied as biocontrol agents. Here, we report mixed-infection studies of neonate larvae of the common cutworm, Agrotis segetum, with two baculoviruses, Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV). By applying quantitative PCR (qPCR) analysis, coinfections of individual larvae were demonstrated, and occlusion body (OB) production within singly infected and coinfected larvae was determined in individual larvae. Mixtures of viruses did not lead to changes in mortality rates compared with rates of single-virus treatments, indicating an independent action within host larvae under our experimental conditions. AgseNPV-B-infected larvae showed an increase in OB production during 2 weeks of infection, whereas the number of AgseGV OBs did not change from the first week to the second week. Fewer OBs of both viruses were produced in coinfections than in singly infected larvae, suggesting a competition of the two viruses for larval resources. Hence, no functional or economic advantage could be inferred from larval mortality and OB production from mixed infections of A. segetum larvae with AgseNPV-B and AgseGV.

  • The Agrotis baculovirus complex: multiple viruses for multiple pests
    , 2011
    Co-Authors: Jörg T. Wennmann, Gianpiero Gueli Alletti, Wael El-menofy, Waly Essam, Naglaa A. Abdallah, Johannes A. Jehle
    Abstract:

    Larvae of the genus Agrotis (Lepidoptera: Noctuidae) are known to be severe soil pests on a wide range of field crops and vegetables in Europe, Asia and Africa. Agrotis spec. are highly susceptible for a broad number of baculoviruses and in the past, two Alphabaculoviruses (AgseNPV-A (Polish strain), AgseNPV-B (Oxford strain)) and one Betabaculovirus (AgseGV) were isolated from the common cutworm A. segetum. From larvae of the black cutworm A. ipsilon another Alphabaculovirus, Agrotis ipsilon nucleopolyhedrovirus (AgipNPV, Illinois strain), was isolated. Bioassay analysis demonstrated the cross-infectivity of all four baculoviruses for both hosts, which made them potential biocontrol agents for the control of cutworms. Especially in terms of resistance management the usage of a combination of different baculoviruses is regarded to be useful. In order to develop methods for identification of the different viruses we developed a multiplex polymerase chain reaction (PCR) and quantitative PCR (qPCR) based method. The genome of AgseNPV-B was completely sequenced and a comparative genome analysis of AgseNPV-B, AgseNPV-A and AgipNPV was conducted. Phylogenetic analysis confirmed the close relationship of AgseNPV-B and AgipNPV by a high sequence similarity, although the genome length and number of open reading frames (ORF) of AgseNPV-B and AgseNPV-A were more alike. For biological characterization bioassays and the determination of the median lethlethal dose (LC50) of AgipNPV and AgseNPV for their common host A. segetum, were performed. This work is the basis to analyze the molecular and cellular interaction of these viruses in mixed infections and to optimize the application of these viruses for Agrotis

Otto Vostrowsky – One of the best experts on this subject based on the ideXlab platform.

Just M. Vlak – One of the best experts on this subject based on the ideXlab platform.

  • Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland.
    Journal of invertebrate pathology, 2005
    Co-Authors: Agata K. Jakubowska, Monique M. Van Oers, Jadwiga Ziemnicka, Jerzy J. Lipa, Just M. Vlak
    Abstract:

    The turnip moth, Agrotis segetum (Lepidoptera, Noctuidae), is an important pest insect in Europe, Asia, and Africa. We have genetically characterized and classified a nucleopolyhedrovirus isolated from A. segetum larvae in Poland (AgseNPV-P). The restriction pattern of AgseNPV-P was distinct from an isolate from England/France (AgseNPV-UK and AgseNPV-F). Sequence analysis of three conserved baculovirus genes, polyhedrin, lef-8 and pif-2, revealed that AgseNPV-P differs substantially from the already described NPVs isolated from A. segetum and possibly represents a new NPV species. Phylogenetic analysis placed AgseNPV-P among group II NPVs and showed the closest relationship to Agrotis ipsilon (Agip) NPV and Spodoptera exigua (Se) MNPV.

  • Short communication Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland
    , 2005
    Co-Authors: Agata K. Jakubowska, Monique M. Van Oers, Jadwiga Ziemnicka, Jerzy J. Lipa, Just M. Vlak
    Abstract:

    Abstract The turnip moth, Agrotis segetum (Lepidoptera, Noctuidae), is an important pest insect in Europe, Asia, and Africa. We havegenetically characterized and classiWed a nucleopolyhedrovirus isolated from A. segetum larvae in Poland (AgseNPV-P). The restric-tion pattern of AgseNPV-P was distinct from an isolate from England/France (AgseNPV-UK and AgseNPV-F). Sequence analysisof three conserved baculovirus genes, polyhedrin , lef-8 and pif-2 , revealed that AgseNPV-P diVers substantially from the alreadydescribed NPVs isolated from A. segetum and possibly represents a new NPV species. Phylogenetic analysis placed AgseNPV-Pamong group II NPVs and showed the closest relationship to Agrotis ipsilon (Agip) NPV and Spodoptera exigua (Se) MNPV. 2005 Elsevier Inc. All rights reserved. Keywords: Agrotis segetum ; Nucleopolyhedrovirus; AsMNPV; Phylogeny; Taxonomy The turnip moth Agrotis (Scotia) segetum Dennisand SchiVenm:ller (Lepidoptera, Noctuidae) is a seriouspest occurring throughout Europe, Asia, and part ofAfrica. The larvae belong to cutworms (noctuids spend-ing a large portion of their time in the soil, Bourner andCory, 2004) and feed on many vegetable and Weld cropsincluding corn, rape, beet, potatoes, cabbage, cereals,tobacco, vine, and many others (IgnoVo and Garcia,1979).