The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform
Xianliang Zheng - One of the best experts on this subject based on the ideXlab platform.
-
a novel and robust recombinant pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a thermomyces lanuginosus lipase preparation characterization and application in biodiesel production
Bioresource Technology, 2014Co-Authors: Jinyong Yan, Xianliang ZhengAbstract:A novel and robust recombinant Pichia pastoris yeast whole cell catalyst (WCC) with functional intracellular expression of Thermomyces lanuginosus lipase (Tll) was constructed and characterized for biodiesel production from waste cooking oils. This permeabilized WCC was able to convert waste cooking oils to biodiesel with 82% yield within 84 h at 6% dosage whole cells. The WCC showed two fold catalytic activity of 0.73 U/mg DCW compared to its commercial counterpart Lipozyme TLIM (immobilized Tll). Short chain Alcohol Tolerance of this WCC was significantly improved compared to Lipozyme TLIM. This beneficial property enabled it to catalyze biodiesel production efficiently with one step addition of methanol. The reusability of this biocatalyst retained 78% activity after three batch cycles. This easily prepared and cost-effective WCC showed better catalytic performance than Lipozyme TLIM with respect to biodiesel yield and productivity, thus suggesting a promising cost-effective biocatalyst for biodiesel production.
-
a novel and robust recombinant pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a thermomyces lanuginosus lipase preparation characterization and application in biodiesel production
Bioresource Technology, 2014Co-Authors: Xianliang Zheng, Shengying LiAbstract:A novel and robust recombinant Pichia pastoris yeast whole cell catalyst (WCC) with functional intracellular expression of Thermomyces lanuginosus lipase (Tll) was constructed and characterized for biodiesel production from waste cooking oils. This permeabilized WCC was able to convert waste cooking oils to biodiesel with 82% yield within 84 h at 6% dosage whole cells. The WCC showed two fold catalytic activity of 0.73 U/mg DCW compared to its commercial counterpart Lipozyme TLIM (immobilized Tll). Short chain Alcohol Tolerance of this WCC was significantly improved compared to Lipozyme TLIM. This beneficial property enabled it to catalyze biodiesel production efficiently with one step addition of methanol. The reusability of this biocatalyst retained 78% activity after three batch cycles. This easily prepared and cost-effective WCC showed better catalytic performance than Lipozyme TLIM with respect to biodiesel yield and productivity, thus suggesting a promising cost-effective biocatalyst for biodiesel production. (C) 2013 Elsevier Ltd. All rights reserved.
Rebecca Shannon Spicer - One of the best experts on this subject based on the ideXlab platform.
-
highway crash costs in the united states by driver age blood Alcohol level victim age and restraint use
Accident Analysis & Prevention, 1998Co-Authors: Ted R Miller, Diane C Lestina, Rebecca Shannon SpicerAbstract:This paper estimates 1993 U.S. highway crash incidence and costs by driver age, Alcohol use, victim age, occupant status, and restraint use. Notable findings are: (1) crash costs of novice drivers are high enough to yield preliminary benefit-cost ratios around 4-8 for a provisional licensing system that restricts driving after midnight and 11 for zero Alcohol Tolerance for young drivers with violators receiving a 6-month suspension; (2) the costs to people other than the intoxicated driver per mile driven at BACs of 0.08-0.099% exceed the value of driver mobility; (3) the safety costs of drunk driving appear to exceed $5.80 per mile, compared with $2.50 per mile driven at BACs of 0.08-0.099%, and $0.11 per mile driven sober; (4) highway crashes cause an estimated 3.2% of U.S. medical spending, including more than 14% of medical spending for ages 15-24; (5) ignoring crash-involved occupants whose restraint use is unknown, the 13% of occupants who police reported were traveling unrestrained accounted for an estimated 42% of the crash costs; and (6) if these unrestrained occupants buckled up, the medical costs of crashes would decline by an estimated 18% (almost $4 billion annually) and the comprehensive costs by 24%.
-
highway crash costs in the united states by driver age blood Alcohol level victim age and restraint use
PROCEEDINGS OF THE 40TH ANNUAL CONFERENCE OF THE ASSOCIATION FOR THE ADVANCEMENT OF AUTOMOTIVE MEDICINE VANCOUVER BRITISH COLUMBIA CANADA OCTOBER 7-9 , 1996Co-Authors: Ted R Miller, Diane C Lestina, Rebecca Shannon SpicerAbstract:This paper estimates 1993 United States highway crash incidence and costs by driver age, Alcohol use, victim age, occupant status, and restraint use. It appears that crash costs of novice drivers are high enough to yield preliminary benefit-cost ratios around 10 to 17 for a provisional licensing system that restricts driving after midnight, and 25 for zero Alcohol Tolerance for young drivers with violators receiving a 6-month suspension.The costs to people other than the intoxicated driver per mile driven at blood Alcohol contents (BACs) of .08-.099% exceed the value of driver mobility. The safety costs of drunk driving appear to exceed 5.50 dollars per mile, compared with 2.40 dollars per mile driven at BACs of .08-.099%, and .11 dollar per mile driven sober. It also appears that highway crashes cause an estimated 3.4% of USA medical spending, including more than 12% of medical spending for ages 15-24. Ignoring crash-involved occupants whose restraint use is unknown, the 13% of occupants who police reported were travelling unrestrained accounted for an estimated 41% of the crash costs.If these unrestrained occupants buckled up, the medical costs of crashes would decline by an estimated 20% (almost 4 billion dollars annually) and the comprehensive costs by 27%. (A) For the covering abstract of the conference see IRRD 893891.
Steven N Treistman - One of the best experts on this subject based on the ideXlab platform.
-
bk channels mediators and models for Alcohol Tolerance
Trends in Neurosciences, 2009Co-Authors: Steven N Treistman, Gilles E MartinAbstract:Enhanced acute Tolerance predicts Alcohol abuse. We describe work on the role of the calcium- and voltage-gated BK channel in Alcohol Tolerance, highlighting the lipid environment, BK protein isoform selection and auxiliary BK channel proteins. We show how ethanol, which had the reputation of a nonspecific membrane perturbant, is now being examined at realistic concentrations with cutting-edge techniques, providing novel molecular targets for therapeutic approaches to Alcoholism. Addictive disorders impact our emotional, physical and financial status, and burden our healthcare system. Although Alcohol is the focus of this review, it is highly probable, given the common neural and biochemical pathways used by drugs of abuse, that the findings described here will also apply to other drugs.
-
posttranscriptional regulation of bk channel splice variant stability by mir 9 underlies neuroadaptation to Alcohol
Neuron, 2008Co-Authors: Andrzej Z Pietrzykowski, Gilles E Martin, Ryan M Friesen, Sylvie I Puig, Cheryl L Nowak, Patricia M Wynne, Hava T Siegelmann, Steven N TreistmanAbstract:Tolerance represents a critical component of addiction. The large-conductance calcium- and voltage-activated potassium channel (BK) is a well-established Alcohol target, and an important element in behavioral and molecular Alcohol Tolerance. We tested whether microRNA, a newly discovered class of gene expression regulators, plays a role in the development of Tolerance. We show that in adult mammalian brain, Alcohol upregulates microRNA miR-9 and mediates posttranscriptional reorganization in BK mRNA splice variants by miR-9-dependent destabilization of BK mRNAs containing 3'UTRs with a miR-9 Recognition Element (MRE). Different splice variants encode BK isoforms with different Alcohol sensitivities. Computational modeling indicates that this miR-9-dependent mechanism contributes to Alcohol Tolerance. Moreover, this mechanism can be extended to include regulation of additional miR-9 targets relevant to Alcohol abuse. Our results describe a mechanism of multiplex regulation of stability of alternatively spliced mRNA by microRNA in drug adaptation and neuronal plasticity.
-
acute Alcohol Tolerance is intrinsic to the bkca protein but is modulated by the lipid environment
Journal of Biological Chemistry, 2008Co-Authors: Chunbo Yuan, Andrzej Z Pietrzykowski, Robert J Oconnell, Andrew Wilson, Steven N TreistmanAbstract:Abstract Ethanol Tolerance, in which exposure leads to reduced sensitivity, is an important component of Alcohol abuse and addiction. The molecular mechanisms underlying this process remain poorly understood. The BKCa channel plays a central role in the behavioral response to ethanol in Caenorhabditis elegans ( Davies, A. G., Pierce-Shimomura, J. T., Kim, H., VanHoven, M. K., Thiele, T. R., Bonci, A., Bargmann, C. I., and McIntire, S. L. (2003) Cell 115, 655-666) and Drosophila (Cowmeadow, R. B., Krishnan, H. R., and Atkinson, N. S. (2005) Alcohol. Clin. Exp. Res. 29, 1777-1786) . In neurons, ethanol Tolerance in BKCa channels has two components: a reduced number of membrane channels and decreased potentiation of the remaining channels (Pietrzykowski, A. Z., Martin, G. E., Puig, S. I., Knott, T. K., Lemos, J. R., and Treistman, S. N. (2004) J. Neurosci. 24, 8322-8332) . Here, heterologous expression coupled with planar bilayer techniques examines two additional aspects of Tolerance in human BKCa channels. 1) Is acute Tolerance observed in a single channel protein complex within a lipid environment reduced to only two lipids? 2) Does lipid bilayer composition affect the appearance of acute Tolerance? We found that Tolerance was observable in BKCa channels in membrane patches pulled from HEK cells and when they are placed into reconstituted 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylserine membranes. Furthermore, altering bilayer thickness by incorporating the channel into lipid mixtures of 1,2-dioleoyl-3-phosphatidylethanolamine with phosphatidylcholines of increasing chain length, or with sphingomyelin, strongly affected the sensitivity of the channel, as well as the time course of the acute response. Ethanol sensitivity changed from a strong potentiation in thin bilayers to inhibition in thick sphingomyelin/1,2-dioleoyl-3-phosphatidylethanolamine bilayers. Thus, Tolerance can be an intrinsic property of the channel protein-lipid complex, and bilayer thickness plays an important role in shaping the pattern of response to ethanol. As a consequence of these findings the protein-lipid complex should be treated as a unit when studying ethanol action.
-
Alcohol Tolerance in Large-Conductance, Calcium-Activated Potassium Channels of CNS Terminals Is Intrinsic and Includes Two Components: Decreased Ethanol Potentiation and Decreased Channel Density
2004Co-Authors: Andrzej Z Pietrzykowski, Gilles E Martin, Sylvie I Puig, Thomas K. Knott, Jose R. Lemos, Steven N TreistmanAbstract:Tolerance is an important element of drug addiction and provides a model for understanding neuronal plasticity. The hypothalamic– neurohypophysial system (HNS) is an established preparation in which to study the actions of Alcohol. Acute application of Alcohol to the rat neurohypophysis potentiates large-conductance calcium-sensitive potassium channels (BK), contributing to inhibition of hormone secretion. A cultured HNS explant from adult rat was used to explore the molecular mechanisms of BK Tolerance after prolonged Alcohol exposure. Ethanol Tolerance was intrinsic to the HNS and consisted of: (1) decreased BK potentiation by ethanol, complete within 12 min of exposure, and (2) decreased current density, which was not complete until 24 hr after exposure, indicating that the two components of Tolerance represent distinct processes. Single-channel properties were not affected by chronic exposure, suggesting that decreased current density resulted from downregulation of functional channels in the membrane. Indeed, we observed decreased immunolabeling against the BK �-subunit on the surface of tolerant terminals. Analysis using confocal microscopy revealed a reduction of BK channel clustering, likely associated with the internalization of the channel. Key words: addiction; Alcohol; ethanol; explant; Tolerance; BK channel; terminal
Shengying Li - One of the best experts on this subject based on the ideXlab platform.
-
a novel and robust recombinant pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a thermomyces lanuginosus lipase preparation characterization and application in biodiesel production
Bioresource Technology, 2014Co-Authors: Xianliang Zheng, Shengying LiAbstract:A novel and robust recombinant Pichia pastoris yeast whole cell catalyst (WCC) with functional intracellular expression of Thermomyces lanuginosus lipase (Tll) was constructed and characterized for biodiesel production from waste cooking oils. This permeabilized WCC was able to convert waste cooking oils to biodiesel with 82% yield within 84 h at 6% dosage whole cells. The WCC showed two fold catalytic activity of 0.73 U/mg DCW compared to its commercial counterpart Lipozyme TLIM (immobilized Tll). Short chain Alcohol Tolerance of this WCC was significantly improved compared to Lipozyme TLIM. This beneficial property enabled it to catalyze biodiesel production efficiently with one step addition of methanol. The reusability of this biocatalyst retained 78% activity after three batch cycles. This easily prepared and cost-effective WCC showed better catalytic performance than Lipozyme TLIM with respect to biodiesel yield and productivity, thus suggesting a promising cost-effective biocatalyst for biodiesel production. (C) 2013 Elsevier Ltd. All rights reserved.
Ted R Miller - One of the best experts on this subject based on the ideXlab platform.
-
highway crash costs in the united states by driver age blood Alcohol level victim age and restraint use
Accident Analysis & Prevention, 1998Co-Authors: Ted R Miller, Diane C Lestina, Rebecca Shannon SpicerAbstract:This paper estimates 1993 U.S. highway crash incidence and costs by driver age, Alcohol use, victim age, occupant status, and restraint use. Notable findings are: (1) crash costs of novice drivers are high enough to yield preliminary benefit-cost ratios around 4-8 for a provisional licensing system that restricts driving after midnight and 11 for zero Alcohol Tolerance for young drivers with violators receiving a 6-month suspension; (2) the costs to people other than the intoxicated driver per mile driven at BACs of 0.08-0.099% exceed the value of driver mobility; (3) the safety costs of drunk driving appear to exceed $5.80 per mile, compared with $2.50 per mile driven at BACs of 0.08-0.099%, and $0.11 per mile driven sober; (4) highway crashes cause an estimated 3.2% of U.S. medical spending, including more than 14% of medical spending for ages 15-24; (5) ignoring crash-involved occupants whose restraint use is unknown, the 13% of occupants who police reported were traveling unrestrained accounted for an estimated 42% of the crash costs; and (6) if these unrestrained occupants buckled up, the medical costs of crashes would decline by an estimated 18% (almost $4 billion annually) and the comprehensive costs by 24%.
-
highway crash costs in the united states by driver age blood Alcohol level victim age and restraint use
PROCEEDINGS OF THE 40TH ANNUAL CONFERENCE OF THE ASSOCIATION FOR THE ADVANCEMENT OF AUTOMOTIVE MEDICINE VANCOUVER BRITISH COLUMBIA CANADA OCTOBER 7-9 , 1996Co-Authors: Ted R Miller, Diane C Lestina, Rebecca Shannon SpicerAbstract:This paper estimates 1993 United States highway crash incidence and costs by driver age, Alcohol use, victim age, occupant status, and restraint use. It appears that crash costs of novice drivers are high enough to yield preliminary benefit-cost ratios around 10 to 17 for a provisional licensing system that restricts driving after midnight, and 25 for zero Alcohol Tolerance for young drivers with violators receiving a 6-month suspension.The costs to people other than the intoxicated driver per mile driven at blood Alcohol contents (BACs) of .08-.099% exceed the value of driver mobility. The safety costs of drunk driving appear to exceed 5.50 dollars per mile, compared with 2.40 dollars per mile driven at BACs of .08-.099%, and .11 dollar per mile driven sober. It also appears that highway crashes cause an estimated 3.4% of USA medical spending, including more than 12% of medical spending for ages 15-24. Ignoring crash-involved occupants whose restraint use is unknown, the 13% of occupants who police reported were travelling unrestrained accounted for an estimated 41% of the crash costs.If these unrestrained occupants buckled up, the medical costs of crashes would decline by an estimated 20% (almost 4 billion dollars annually) and the comprehensive costs by 27%. (A) For the covering abstract of the conference see IRRD 893891.