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Abal Inti, Henrys Alain - One of the best experts on this subject based on the ideXlab platform.
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Determinación de Aldicarb por cromatografia líquida acoplada a masas LC/MS en fresas obtenidas de los principales mercados de abasto de Lima Metropolitana
'Universidad Inca Garcilaso de la Vega', 2019Co-Authors: Dietz Maravi, Robert Anthony, Abal Inti, Henrys AlainAbstract:The research was aimed at determining the levels of Aldicarb in strawberries in the main supply markets of Lima. To carry out the determination of Aldicarb, were the samples collected at different centres of supplies in Lima, we obtained 10 random samples which each of them contained 1kg for each sample, from the provinces of Huaral and Lima; then, after obtaining the samples, they were stored at 0 – 8 ° C, as requirement of cold chain, where they remained for a week at the same temperature. After the storage, were the samples by means of cold chain to the laboratories of SENASA, where we proceeded to determine the concentrations of Aldicarb in the 10 samples of strawberries using the technique of liquid chromatography coupled to mass LC/MS, where no detectable levels of Aldicarb surpluses, concluding, therefore, that the use of Aldicarb in strawberry crops and agriculture is located within the ranges allowed by the Supreme Decree Nº015-2015-MINAM, it establishes the modification of National Environmental Quality Standards for water and establishes complementary provisions for their application.TesisLa investigación realizada tuvo como objetivo determinar los niveles de Aldicarb en fresas en los principales mercados de abastos de Lima. Para llevar a cabo la determinación de Aldicarb, se llevaron las muestras recolectadas en distintos centros de abastos de Lima, se obtuvo 10 muestras aleatorias de las cuales cada una de ellas contenían 1kg por cada muestra, provenientes de las provincias de Huaral y de Huaraz; seguidamente, luego de obtener las muestras, se almacenaron a 0 – 8ºC, como requerimiento de cadena de frio, donde se mantuvieron durante una semana a la misma temperatura. Luego del almacenamiento, se llevaron las muestras mediante cadena de frío a los laboratorios de SENASA, donde se procedió a determinar las concentraciones de Aldicarb en las 10 muestras de fresas mediante la técnica de cromatografía líquida acoplada a masas LC/MS, donde no se detectaron niveles de Aldicarb excedentes, concluyendo, por lo tanto, que el uso de Aldicarb en los cultivos de fresas y agricultura se encuentra dentro de los rangos permitidos por el Decreto Supremo Nº015-2015-MINAM, el cual establece la modificación de los estándares nacionales de calidad ambiental para agua y establecen disposiciones complementarias para su aplicación
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Determinación de Aldicarb por cromatografia líquida acoplada a masas LC/MS en fresas obtenidas de los principales mercados de abasto de Lima Metropolitana
'Universidad Inca Garcilaso de la Vega', 2019Co-Authors: Abal Inti, Henrys Alain, Dietz Maravi, Robert AnthonyAbstract:La investigación realizada tuvo como objetivo determinar los niveles de Aldicarb en fresas en los principales mercados de abastos de Lima. Para llevar a cabo la determinación de Aldicarb, se llevaron las muestras recolectadas en distintos centros de abastos de Lima, se obtuvo 10 muestras aleatorias de las cuales cada una de ellas contenían 1kg por cada muestra, provenientes de las provincias de Huaral y de Huaraz; seguidamente, luego de obtener las muestras, se almacenaron a 0 – 8ºC, como requerimiento de cadena de frio, donde se mantuvieron durante una semana a la misma temperatura. Luego del almacenamiento, se llevaron las muestras mediante cadena de frío a los laboratorios de SENASA, donde se procedió a determinar las concentraciones de Aldicarb en las 10 muestras de fresas mediante la técnica de cromatografía líquida acoplada a masas LC/MS, donde no se detectaron niveles de Aldicarb excedentes, concluyendo, por lo tanto, que el uso de Aldicarb en los cultivos de fresas y agricultura se encuentra dentro de los rangos permitidos por el Decreto Supremo Nº015-2015-MINAM, el cual establece la modificación de los estándares nacionales de calidad ambiental para agua y establecen disposiciones complementarias para su aplicación.The research was aimed at determining the levels of Aldicarb in strawberries in the main supply markets of Lima. To carry out the determination of Aldicarb, were the samples collected at different centres of supplies in Lima, we obtained 10 random samples which each of them contained 1kg for each sample, from the provinces of Huaral and Lima; then, after obtaining the samples, they were stored at 0 – 8 ° C, as requirement of cold chain, where they remained for a week at the same temperature. After the storage, were the samples by means of cold chain to the laboratories of SENASA, where we proceeded to determine the concentrations of Aldicarb in the 10 samples of strawberries using the technique of liquid chromatography coupled to mass LC/MS, where no detectable levels of Aldicarb surpluses, concluding, therefore, that the use of Aldicarb in strawberry crops and agriculture is located within the ranges allowed by the Supreme Decree Nº015-2015-MINAM, it establishes the modification of National Environmental Quality Standards for water and establishes complementary provisions for their application
Dietz Maravi, Robert Anthony - One of the best experts on this subject based on the ideXlab platform.
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Determinación de Aldicarb por cromatografia líquida acoplada a masas LC/MS en fresas obtenidas de los principales mercados de abasto de Lima Metropolitana
'Universidad Inca Garcilaso de la Vega', 2019Co-Authors: Dietz Maravi, Robert Anthony, Abal Inti, Henrys AlainAbstract:The research was aimed at determining the levels of Aldicarb in strawberries in the main supply markets of Lima. To carry out the determination of Aldicarb, were the samples collected at different centres of supplies in Lima, we obtained 10 random samples which each of them contained 1kg for each sample, from the provinces of Huaral and Lima; then, after obtaining the samples, they were stored at 0 – 8 ° C, as requirement of cold chain, where they remained for a week at the same temperature. After the storage, were the samples by means of cold chain to the laboratories of SENASA, where we proceeded to determine the concentrations of Aldicarb in the 10 samples of strawberries using the technique of liquid chromatography coupled to mass LC/MS, where no detectable levels of Aldicarb surpluses, concluding, therefore, that the use of Aldicarb in strawberry crops and agriculture is located within the ranges allowed by the Supreme Decree Nº015-2015-MINAM, it establishes the modification of National Environmental Quality Standards for water and establishes complementary provisions for their application.TesisLa investigación realizada tuvo como objetivo determinar los niveles de Aldicarb en fresas en los principales mercados de abastos de Lima. Para llevar a cabo la determinación de Aldicarb, se llevaron las muestras recolectadas en distintos centros de abastos de Lima, se obtuvo 10 muestras aleatorias de las cuales cada una de ellas contenían 1kg por cada muestra, provenientes de las provincias de Huaral y de Huaraz; seguidamente, luego de obtener las muestras, se almacenaron a 0 – 8ºC, como requerimiento de cadena de frio, donde se mantuvieron durante una semana a la misma temperatura. Luego del almacenamiento, se llevaron las muestras mediante cadena de frío a los laboratorios de SENASA, donde se procedió a determinar las concentraciones de Aldicarb en las 10 muestras de fresas mediante la técnica de cromatografía líquida acoplada a masas LC/MS, donde no se detectaron niveles de Aldicarb excedentes, concluyendo, por lo tanto, que el uso de Aldicarb en los cultivos de fresas y agricultura se encuentra dentro de los rangos permitidos por el Decreto Supremo Nº015-2015-MINAM, el cual establece la modificación de los estándares nacionales de calidad ambiental para agua y establecen disposiciones complementarias para su aplicación
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Determinación de Aldicarb por cromatografia líquida acoplada a masas LC/MS en fresas obtenidas de los principales mercados de abasto de Lima Metropolitana
'Universidad Inca Garcilaso de la Vega', 2019Co-Authors: Abal Inti, Henrys Alain, Dietz Maravi, Robert AnthonyAbstract:La investigación realizada tuvo como objetivo determinar los niveles de Aldicarb en fresas en los principales mercados de abastos de Lima. Para llevar a cabo la determinación de Aldicarb, se llevaron las muestras recolectadas en distintos centros de abastos de Lima, se obtuvo 10 muestras aleatorias de las cuales cada una de ellas contenían 1kg por cada muestra, provenientes de las provincias de Huaral y de Huaraz; seguidamente, luego de obtener las muestras, se almacenaron a 0 – 8ºC, como requerimiento de cadena de frio, donde se mantuvieron durante una semana a la misma temperatura. Luego del almacenamiento, se llevaron las muestras mediante cadena de frío a los laboratorios de SENASA, donde se procedió a determinar las concentraciones de Aldicarb en las 10 muestras de fresas mediante la técnica de cromatografía líquida acoplada a masas LC/MS, donde no se detectaron niveles de Aldicarb excedentes, concluyendo, por lo tanto, que el uso de Aldicarb en los cultivos de fresas y agricultura se encuentra dentro de los rangos permitidos por el Decreto Supremo Nº015-2015-MINAM, el cual establece la modificación de los estándares nacionales de calidad ambiental para agua y establecen disposiciones complementarias para su aplicación.The research was aimed at determining the levels of Aldicarb in strawberries in the main supply markets of Lima. To carry out the determination of Aldicarb, were the samples collected at different centres of supplies in Lima, we obtained 10 random samples which each of them contained 1kg for each sample, from the provinces of Huaral and Lima; then, after obtaining the samples, they were stored at 0 – 8 ° C, as requirement of cold chain, where they remained for a week at the same temperature. After the storage, were the samples by means of cold chain to the laboratories of SENASA, where we proceeded to determine the concentrations of Aldicarb in the 10 samples of strawberries using the technique of liquid chromatography coupled to mass LC/MS, where no detectable levels of Aldicarb surpluses, concluding, therefore, that the use of Aldicarb in strawberry crops and agriculture is located within the ranges allowed by the Supreme Decree Nº015-2015-MINAM, it establishes the modification of National Environmental Quality Standards for water and establishes complementary provisions for their application
Patiño-reyes Nancy - One of the best experts on this subject based on the ideXlab platform.
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Intoxicación letal con Aldicarb: análisis de sangre post mortem mediante LC-ESI-MS/MS
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina, 2015Co-Authors: Mariño-gaviria, Diana Jazmín, Patiño-reyes NancyAbstract:Antecedentes. El Aldicarb es un plaguicida carbamato de alta toxicidad asociado a intoxicaciones agudas fatales en el ser humano. Su mecanismo de acción consiste en la inhibición de la enzima acetilcolinesterasa (AChE) que ocasiona la acumulación del neurotransmisor acetilcolina en la hendidura sináptica. Esta acumulación provoca síntomas colinérgicos y, dependiendo de la dosis de exposición, puede paralizar los sistemas respiratorio y nervioso hasta llegar a la muerte.Objetivo. Determinar el nivel de Aldicarb en sangre post mortem en casos de intoxicación aguda.Materiales y métodos. Investigación de tipo experimental empleando un cromatógrafo líquido con espectrometría de masas, con ionización electrospray y análisis en modo tándem (LC-ESI-MS/MS). Los estándares de Aldicarb y el Aldicarb-d3 fueron comprados de Dr. Ehrenstorfer GmbH.El método consiste en una precipitación de proteínas de la sangre y su posterior análisis por LC-ESI-MS/MS, utilizando el Aldicarb-d3 como estándar interno. El método fue aplicado a siete casos de intoxicación letal por presunta acción del Aldicarb.Resultados. El Aldicarb se encontró en la sangre de seis de los casos estudiados, en niveles desde 0.12 a 1.90 µg/mL. Solo en uno de los casos no se detectó Aldicarb. En cuanto la presunta manera de muerte, en seis de los casos analizados fue el suicidio y en un caso se clasificó como muerte en estudio.Conclusiones. Los resultados obtenidos con la metodología analítica y la técnica LC-ESI-MS/MS son satisfactorios en términos de la determinación cuantitativa de Aldicarb en sangre total post mortem. La aplicación de la metodología descrita en toxicología forense evidencia el empleo de este plaguicida en actos suicidas.Background. Aldicarb is a high toxicity carbamate pesticide associated to human fatal acute intoxications. Its mechanism of action consists of the inhibition of the acetylcholinesterase enzyme (AChE), which induces the accumulation of the neurotransmitter acetylcholine in the synaptic cleft. This accumulation induces cholinergic symptoms and, depending on the exposition dose, it can paralyze the respiratory and nervous systems, leading to death.Objective. To determine Aldicarb levels in post mortem blood in cases of acute intoxication.Materials and methods. An experimental research was conducted using liquid chromatography tandem-mass spectrometry (LC-ESI-MS/MS) with electrospray ionization. The Aldicarb and Aldicarb-d3 standards were purchased from Dr. Ehrenstorfer GmbH corporation.This method carries out a protein precipitation of blood and its analysis using LC-ESI-MS/MS, using Aldicarb-d3 as internal standard. This method was applied to seven cases of fatal intoxication by presumable action of Aldicarb.Results. Aldicarb was found in six of the studied cases on levels between 0.12 and 1.90 µg/mL. Aldicarb was not detected in blood only in one case. Six of the cases were associated to suicide as a manner of death and in one of them it remained under study.Conclusions. The results obtained with the analytical methodology and the use of the LC-ESI-MS/MS technique are satisfactory in terms of the quantitative determination of Aldicarb in post mortem total blood. The application of the described methodology in forensic toxicology evidences the use of this pesticide in suicidal practices
Nancy Patiño Reyes - One of the best experts on this subject based on the ideXlab platform.
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Intoxicación letal con Aldicarb: análisis de sangre post mortem mediante LC-ESI-MS/MS
Universidad Nacional de Colombia, 2015Co-Authors: Diana Jazmín Mariño Gaviria, Nancy Patiño ReyesAbstract:El Aldicarb es un plaguicida carbamato de alta toxicidad asociado a intoxicaciones agudas fatales en Colombia. El presente estudio describe un método analítico para la determinación cuantitativa de Aldicarb en sangre postmortem. Básicamente el método consiste en una precipitación de proteínas de la sangre para luego ser analizada por LC-ESI-MS/MS utilizando el Aldicarb-d3 como estándar interno. El método desarrollado en este trabajo fue aplicado a siete casos de intoxicación letal presuntamente con este plaguicida. El Aldicarb se encontró en sangre en niveles desde 0,10 y 2,5 µg/mL. De los resultados obtenidos no sólo se expone la utilidad de emplear LC-ESI-MS/MS en el análisis de Aldicarb en sangre para su aplicación en toxicología forense con el propósito de contribuir a la búsqueda de la causa de muerte, sino que además evidencia el empleo de este plaguicida en actos suicidas
Adriana De Siqueira - One of the best experts on this subject based on the ideXlab platform.
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poisoning of cats and dogs by the carbamate pesticides Aldicarb and carbofuran
Research in Veterinary Science, 2015Co-Authors: Adriana De Siqueira, Fernanda Auciello Salvagni, Alberto Soiti Yoshida, Vagner Goncalvesjunior, Atilio Sersun Calefi, Andre Rinaldi Fukushima, Helenice De Souza Spinosa, Paulo Cesar MaiorkaAbstract:The intentional and accidental poisoning of animals and people is a threat to public health and safety worldwide. Necropsies and histopathological examinations of 26 cats and 10 dogs poisoned by the carbamates Aldicarb and carbofuran, confirmed by thin layer chromatography (TLC) and high performance liquid chromatography with diode-array detector (HPLC-DAD) were analysed, with variable post mortem interval and conservation of the carcass. Biological matrices were collected for toxicological and histopathological analyses. High performance liquid chromatography with diode-array detector (HPLC-DAD) was utilized to detect Aldicarb and its metabolites, Aldicarb sulphoxide and Aldicarb sulphone, and carbofuran. The variable post mortem interval and the method of conservation of the carcass may be harmful to toxicological, necroscopic and histopathological analyses, that should be performed in order to provide reliable evidences to investigate possible poisoning of animals, which is cruel crime, and are usually linked to domestic or social conflict.
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Evaluation of the toxic effects of carbamates: I. Experimental model in Wistar rats and comparison with the intentional poisoning of cats and dogs; II. Analysis of the stability of the compounds and their effects in the immediate post mortem and in exhumed animals
Universidade de São Paulo, 2015Co-Authors: Adriana De SiqueiraAbstract:A intoxicação intencional de animais e de pessoas é uma ameaça à saúde e à segurança públicas em todo o mundo. No Brasil, os pesticidas da classe dos carbamatos Aldicarbe e carbofurano são facilmente obtidos, e uma única dose letal daqueles é facilmente misturada a alimentos palatáveis e iscas. Foram analisadas as necropsias de 26 gatos e 10 cães, da rotina do Serviço de Patologia Animal da FMVZ-USP, com variados intervalos post mortem e tipos de conservação de carcaça, intoxicados pelos carbamatos Aldicarbe e carbofurano, confirmados pela cromatografia de camada delgada (CCD) e cromatografia líquida de alta performance com detector de arranjo de diodos (CLAE-DAD). Foram colhidas as matrizes biológicas para histopatologia e análise toxicológica. CLAE-DAD foi utilizada para detectar o Aldicarbe e seus metabólitos, Aldicarbe sulfóxido e Aldicarbe sulfona, e carbofurano e seu metabólito 3-hidroxicarbofurano. As análises macroscópica e histopatológica revelaram predominantemente achados congestivos e hemorrágicos, o que pode ser resultante dos efeitos tóxicos dos carbamatos e seus metabólitos. Foram feitos 2 experimentos envolvendo exposição a uma dose única de carbofurano e de Aldicarbe via gavagem em ratos Wistar. No primeiro, no qual foram avaliados os efeitos no post mortem imediato, 30 animais forma separados nos seguintes grupos: Aldicarbe (n=10), carbofurano (n=10) e grupo controle (n=10). Os animais foram filmados para avaliar as alterações clínicas na intoxicação aguda e, quando foram sedados, foi colhido sangue por punção cardíaca para fazer o hemograma, bioquímica sérica, colinesterase e exame toxicológico e, após a eutanásia, foi feita a necropsia e colhido material para o exame histopatológico. Em todos os animais experimentais foi possível ser feita a detecção e a quantificação do Aldicarbe e do carbofurano, além das alterações microscópicas congestivas e hemorrágicas, com alterações em parâmetros hematológicos e bioquímicos. No segundo experimento, foram utilizados 30 animais, e o protocolo experimental foi idêntico ao experimento anterior, exceto que, após o óbito, os animais foram colocados em uma caixa com terra nos seguintes grupos: 1 dia, 3 dias, 5 dias, 7 dias e 10 dias, e em cada dia foram agrupados 2 animais intoxicados e 1 controle, sendo que as necropsias foram feitas nestes intervalos. Foram colhidos cérebro, pulmão, fígado e rim ao exame histopatológico e globo ocular, conteúdo gástrico, fígado e músculo esquelético ao exame toxicológico. Foram observadas as alterações tanatológicas e, em todas as matrizes analisadas, puderam ser quantificados o Aldicarbe e/ou seus metabólitos Aldicarbe sulfóxido e Aldicarbe sulfona, bem como o carbofurano e seu metabólito 3-hidroxicarbofurano. Estes estudos mostram a importância da necropsia e da coleta de matrizes diversas ao exame toxicológico, que são complementares e cruciais na investigação de óbitos decorrentes de intoxicações por praguicidas, tanto no post mortem imediato quanto em exumaçõesThe intentional poisoning of animals and people is a threat to public health and safety worldwide. In Brazil, the carbamate pesticides Aldicarb and carbofuran are easily obtained. A single lethal dose of these carbamates is easily mixed with palatable food or baits. Necropsies and histopathological examinations of 26 cats and 10 dogs poisoned by the carbamates Aldicarb and carbofuran were performed in the Service of Animal Pathology of FMVZ-USP, and the poisoning was confirmed by thin layer chromatography (TLC) and high performance liquid chromatography with diode-array detector (HPLC-DAD), with variable post mortem interval and conservation of the carcass. Biological matrices were collected for histopathological and toxicological analysis. HPLC-DAD was utilised to detect Aldicarb and its metabolites, Aldicarb sulphoxide and Aldicarb sulphone, and carbofuran and its metabolite 3-hydroxycarbofuran. Gross and histopathological evaluations showed mainly congestive and haemorrhagic findings, which may result from the toxic effects of the carbamates and their metabolites. Two experimental protocols were performed in Wistar rats by exposing them to a single oral gavage dose to the carbamates Aldicarb and carbofuran. The first experiment was performed to evaluate the immediate post mortem effects of carbamates, and 30 rats were divided into three groups: Aldicarb (n-=10), carbofuran (n=10) and control (n=10). The animals were filmed to evaluate the clinical signs of poisoning, and they were sedated to collect blood by heart puncture, to perform CBC, serum biochemistry, and to measure serum cholinesterase. After euthanasia, tissues were collected for histopathological evaluation. Aldicarb and carbofuran were detected and quantified in the blood of all experimental groups, and the most common histological changes observed were congestion and haemorrhage. In the second experiment, the experimental protocol was identical to the first one, but the animals were buried in a plastic box with garden soil within 5 groups of 3 animals, two exposed to the same pesticide and 1 control, as follows: day 1, day 3, day 5, day 7 and day 10. The exhumations were performed within these days (1, 3, 5, 7 and 10). We collected brain, lung, liver and kidney to examine the thanatological changes, and the eyeballs, gastric content, liver and skeletal muscle to perform the toxicological screening. Thanatological changes could be observed within and between the groups, and, in all the matrices we could detect Aldicarb and its metabolites Aldicarb sulphoxide and Aldicarb sulphone, and carbofuran and its metabolite 3-hydroxycarbofuran. The studies have shown the importance of the necropsy and the collection of diverse matrices to the toxicological screening, because both are complementary and crucial to the investigation of deaths by pesticides poisoning, even in exhumation, since we could evaluate the thanatological changes and correlate them to the action of the pesticides in the tissue