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Marín Sánchez José - One of the best experts on this subject based on the ideXlab platform.
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Producción de semilla de tomate de cáscara (Physalis ixocarpa Brot.) libre de virus mediante el manejo de insectos vectores.
Colegio de Postgraduados (COLPOS), 2010Co-Authors: Marín Sánchez JoséAbstract:El tomate de cáscara ocupa el cuarto lugar dentro de las hortalizas en México, sin embargo la investigación en esta especie es escasa, particularmente en el proceso de producción de semilla. Este trabajo se realizó en el Campo Experimental del Colegio de Postgraduados-Campus Montecillo y en un invernadero de la Universidad Autónoma Chapingo. Este último se mantuvo libre de áfidos sembrado con tomate de cáscara (Physalis ixocarpa Brot.) variedad CHF1-Chapingo. Los objetivos de este trabajo fueron identificar los virus transmitidos por semilla, conocer el porcentaje de semillas infectadas de plantas asintomáticas mediante el ensayo serológico ligado a enzimas en doble sándwich (DAS-ELISA), así como para evaluar diferentes tratamientos en el manejo de insectos vectores de virus de esta hortaliza, para producir semilla con calidad física, fisiológica, genética y sanitaria libre de los virus Alfalfa mosaic Alfamovirus (virus mosaico de la alfalfa, AMV), Cucumber mosaic cucumovirus (mosaico del pepino, CMV) y Tobacco etch potyvirus (virus jaspeado del tabaco, TEV). Los tratamientos aplicados fueron: 1. Combate convencional de plagas; 2. Manejo biorracional de plagas; 3. Manejo convencional de plagas más aplicaciones de miel al 2 %; 4. Obtención de semilla en invernadero libre de insectos y 5.Testigo. Los resultados indican la presencia de los virus Alfalfa mosaic Alfamovirus (virus mosaico de la alfalfa, AMV), Cucumber mosaic cucumovirus (virus mosaico del pepino, CMV) y Tobacco etch potyvirus (virus jaspeado del tabaco, TEV) en muestras tomadas de plantas con síntomas de virosis, así como en semilla de plantas asintomáticas. Por otra parte, en el invernadero libre de insectos vectores, se logró obtener semilla con mayor calidad genética, física, fisiológica y sanitaria libre de estos virus. _______________ SEED PRODUCTION OF HUSK TOMATO (Physalis ixocarpa Brot.) FREE OF VIRUSES THROUGH OF MANAGEMENT OF INSECT VECTORS. ABSTRACT: The husk tomato occupies the fourth place among the horticultural species. The investigation in this species is still scarce, particularly about the processes of seed production. The experiment was conducted in an experimental plot at Colegio de Postgraduados and at the Universidad Autonoma Chapingo in a greenhouse free of aphids, with husk tomato (Physalis ixocarpa Brot.) Variety CHF1-Chapingo. The purpose of this research was to determine if viruses were transmitted by seeds and to know the transmission through seed of asymptomatic plants and evaluate different treatments to control physical, physiological, and genetic traits, as well as virus-free plants regarding Alfalfa mosaic Alfamovirus (AMV), Cucumber mosaic cucumovirus (CMV) and Tobacco etch potyvirus (TEV). The treatments were: 1. Conventional pest control, 2. Bio rational control, 3. Conventional pest management with a 2 % honey spraying, 4. Seed production in an insect free greenhouse, and 5. Control. The detection of viruses was carried out with double sandwich enzyme-linked immunoadsorbent assay (DAS-ELISA) serological test. The serological analyses indicated presence of mixed infections with the virus Alfalfa mosaic Alfamovirus, Cucumbre mosaic cucumovirus and Tobacco etch potyvirus, in plants with symptoms of viruses and in seeds obtained from asymptomatic plants. The transmission of TEV is reported for the first time by seed in husk tomato. However, in the vector-free greenhouse, it was able to obtain better quality seed genetics, physical, physiological and virus-free plants
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Producción de semilla de tomate de cáscara (Physalis ixocarpa Brot.) libre de virus mediante el manejo de insectos vectores.
Colegio de Postgraduados (COLPOS), 2010Co-Authors: Marín Sánchez JoséAbstract:El tomate de cáscara ocupa el cuarto lugar dentro de las hortalizas en México, sin embargo la investigación en esta especie es escasa, particularmente en el proceso de producción de semilla. Este trabajo se realizó en el Campo Experimental del Colegio de Postgraduados-Campus Montecillo y en un invernadero de la Universidad Autónoma Chapingo. Este último se mantuvo libre de áfidos sembrado con tomate de cáscara (Physalis ixocarpa Brot.) variedad CHF1-Chapingo. Los objetivos de este trabajo fueron identificar los virus transmitidos por semilla, conocer el porcentaje de semillas infectadas de plantas asintomáticas mediante el ensayo serológico ligado a enzimas en doble sándwich (DAS-ELISA), así como para evaluar diferentes tratamientos en el manejo de insectos vectores de virus de esta hortaliza, para producir semilla con calidad física, fisiológica, genética y sanitaria libre de los virus Alfalfa mosaic Alfamovirus (virus mosaico de la alfalfa, AMV), Cucumber mosaic cucumovirus (mosaico del pepino, CMV) y Tobacco etch potyvirus (virus jaspeado del tabaco, TEV). Los tratamientos aplicados fueron: 1. Combate convencional de plagas; 2. Manejo biorracional de plagas; 3. Manejo convencional de plagas más aplicaciones de miel al 2 %; 4. Obtención de semilla en invernadero libre de insectos y 5.Testigo. Los resultados indican la presencia de los virus Alfalfa mosaic Alfamovirus (virus mosaico de la alfalfa, AMV), Cucumber mosaic cucumovirus (virus mosaico del pepino, CMV) y Tobacco etch potyvirus (virus jaspeado del tabaco, TEV) en muestras tomadas de plantas con síntomas de virosis, así como en semilla de plantas asintomáticas. Por otra parte, en el invernadero libre de insectos vectores, se logró obtener semilla con mayor calidad genética, física, fisiológica y sanitaria libre de estos virus. _______________ SEED PRODUCTION OF HUSK TOMATO (Physalis ixocarpa Brot.) FREE OF VIRUSES THROUGH OF MANAGEMENT OF INSECT VECTORS. ABSTRACT: The husk tomato occupies the fourth place among the horticultural species. The investigation in this species is still scarce, particularly about the processes of seed production. The experiment was conducted in an experimental plot at Colegio de Postgraduados and at the Universidad Autonoma Chapingo in a greenhouse free of aphids, with husk tomato (Physalis ixocarpa Brot.) Variety CHF1-Chapingo. The purpose of this research was to determine if viruses were transmitted by seeds and to know the transmission through seed of asymptomatic plants and evaluate different treatments to control physical, physiological, and genetic traits, as well as virus-free plants regarding Alfalfa mosaic Alfamovirus (AMV), Cucumber mosaic cucumovirus (CMV) and Tobacco etch potyvirus (TEV). The treatments were: 1. Conventional pest control, 2. Bio rational control, 3. Conventional pest management with a 2 % honey spraying, 4. Seed production in an insect free greenhouse, and 5. Control. The detection of viruses was carried out with double sandwich enzyme-linked immunoadsorbent assay (DAS-ELISA) serological test. The serological analyses indicated presence of mixed infections with the virus Alfalfa mosaic Alfamovirus, Cucumbre mosaic cucumovirus and Tobacco etch potyvirus, in plants with symptoms of viruses and in seeds obtained from asymptomatic plants. The transmission of TEV is reported for the first time by seed in husk tomato. However, in the vector-free greenhouse, it was able to obtain better quality seed genetics, physical, physiological and virus-free plants.Tesis (Doctorado en Ciencias, especialista en Entomología y Acarología).- Colegio de Postgraduados, 2010.Consejo Nacional de Ciencia y Tecnología (CONACYT)
J R Diazruiz - One of the best experts on this subject based on the ideXlab platform.
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double stranded rna mediated interference with plant virus infection
Journal of Virology, 2001Co-Authors: Francisco Tenllado, J R DiazruizAbstract:Double-stranded RNA (dsRNA) has been shown to play a key role as an inducer of different interference phenomena occurring in both the plant and animal kingdoms. Here, we show that dsRNA derived from viral sequences can interfere with virus infection in a sequence-specific manner by directly delivering dsRNA to leaf cells either by mechanical inoculation or via an Agrobacterium-mediated transient-expression assay. We have successfully interfered with the infection of plants by three viruses belonging to the tobamovirus, potyvirus, and Alfamovirus groups, demonstrating the reliability of the approach. We suggest that the effect mediated by dsRNA in plant virus infection resembles the analogous phenomenon of RNA interference observed in animals. The interference observed is sequence specific, is dose dependent, and is triggered by dsRNA but not singlestranded RNA. Our results support the view that a dsRNA intermediate in virus replication acts as efficient initiator of posttranscriptional gene silencing (PTGS) in natural virus infections, triggering the initiation step of PTGS that targets viral RNA for degradation. Gene sequences derived from different plant viruses have been introduced into a wide variety of plant species to produce transgenic plants protected against virus infection. In a number of cases, it is known that the mechanism of resistance is a posttranscriptional, RNA-mediated process that targets both the viral RNA and the transgene mRNA for degradation in a sequence-specific manner (11, 19). RNA-mediated virus resistance is a manifestation of posttranscriptional gene silencing (PTGS), a more general phenomenon which was first described as a coordinated and reciprocal inactivation of host gene and transgenes encoding the same sense RNA (reviewed in references 33 and 41). More recently, three components in the dynamics of PTGS have been proposed: initiation, propa
Francisco Tenllado - One of the best experts on this subject based on the ideXlab platform.
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double stranded rna mediated interference with plant virus infection
Journal of Virology, 2001Co-Authors: Francisco Tenllado, J R DiazruizAbstract:Double-stranded RNA (dsRNA) has been shown to play a key role as an inducer of different interference phenomena occurring in both the plant and animal kingdoms. Here, we show that dsRNA derived from viral sequences can interfere with virus infection in a sequence-specific manner by directly delivering dsRNA to leaf cells either by mechanical inoculation or via an Agrobacterium-mediated transient-expression assay. We have successfully interfered with the infection of plants by three viruses belonging to the tobamovirus, potyvirus, and Alfamovirus groups, demonstrating the reliability of the approach. We suggest that the effect mediated by dsRNA in plant virus infection resembles the analogous phenomenon of RNA interference observed in animals. The interference observed is sequence specific, is dose dependent, and is triggered by dsRNA but not singlestranded RNA. Our results support the view that a dsRNA intermediate in virus replication acts as efficient initiator of posttranscriptional gene silencing (PTGS) in natural virus infections, triggering the initiation step of PTGS that targets viral RNA for degradation. Gene sequences derived from different plant viruses have been introduced into a wide variety of plant species to produce transgenic plants protected against virus infection. In a number of cases, it is known that the mechanism of resistance is a posttranscriptional, RNA-mediated process that targets both the viral RNA and the transgene mRNA for degradation in a sequence-specific manner (11, 19). RNA-mediated virus resistance is a manifestation of posttranscriptional gene silencing (PTGS), a more general phenomenon which was first described as a coordinated and reciprocal inactivation of host gene and transgenes encoding the same sense RNA (reviewed in references 33 and 41). More recently, three components in the dynamics of PTGS have been proposed: initiation, propa
Kenneth C. Eastwell - One of the best experts on this subject based on the ideXlab platform.
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the complete nucleotide sequenceof prune dwarf ilarvirus rna 1
Archives of Virology, 1997Co-Authors: Christoph Rampitsch, Kenneth C. EastwellAbstract:The sequence of prune dwarf ilarvirus (PDV) RNA-1 has been determined; it consists of 3 374 nucleotides and contains a single open reading frame of 3 168 nucleotides. The putative translation product is 1 055 amino acids in length with a calculated molecular mass of 118.9 kDa. Both the nucleic acid and the translated amino acid sequences show stronger homology to the corresponding RNA-1 and ORF-1 of apple mosaic ilarvirus and alfalfa mosaic Alfamovirus than to spinach latent mosaic ilarvirus or citrus leaf rugose ilarvirus. These findings are consistent with the inclusion of alfalfa mosaic virus in the ilarvirus genus. The reported sequence of PDV RNA-1 and its single ORF conform to the genomic organization typical of the Bromoviridae family.
Christoph Rampitsch - One of the best experts on this subject based on the ideXlab platform.
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the complete nucleotide sequenceof prune dwarf ilarvirus rna 1
Archives of Virology, 1997Co-Authors: Christoph Rampitsch, Kenneth C. EastwellAbstract:The sequence of prune dwarf ilarvirus (PDV) RNA-1 has been determined; it consists of 3 374 nucleotides and contains a single open reading frame of 3 168 nucleotides. The putative translation product is 1 055 amino acids in length with a calculated molecular mass of 118.9 kDa. Both the nucleic acid and the translated amino acid sequences show stronger homology to the corresponding RNA-1 and ORF-1 of apple mosaic ilarvirus and alfalfa mosaic Alfamovirus than to spinach latent mosaic ilarvirus or citrus leaf rugose ilarvirus. These findings are consistent with the inclusion of alfalfa mosaic virus in the ilarvirus genus. The reported sequence of PDV RNA-1 and its single ORF conform to the genomic organization typical of the Bromoviridae family.