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Leonid M Zakharchuk - One of the best experts on this subject based on the ideXlab platform.
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The dependence of intracellular ATP level on the nutrition mode of the acidophilic bacteria Sulfobacillus thermotolerans and Alicyclobacillus tolerans
Microbiology, 2007Co-Authors: I A Tsaplina, T I Bogdanova, E. N. Krasil’nikova, Leonid M Zakharchuk, A. E. Zhuravleva, A. D. Ismailov, Grigorii I KaravaikoAbstract:The dynamics of the ATP pool in the aerobic spore-forming acidothermophilic mixotrophic bacteria Sulfobacillus thermotolerans Kr1^T and Alicyclobacillus tolerans K1^T were studied in the course of their chemolithoheterotrophic, chemoorganoheterotrophic, and chemolithoautotrophic growth. It was established that, during mixotrophic growth, the maximum ATP concentrations in the cells of S. thermotolerans Kr1 and A. tolerans K1 were 3.8 and 0.6 nmol/mg protein, respectively. The ATP concentrations in sulfobacilli and alicyclobacilli during organotrophic growth were 2.2 and 3.1 nmol/mg protein, respectively. In the cells of the obligately heterotrophic bacterium Alicyclobacillus cycloheptanicus 4006^T, the maximum ATP concentration was several times higher and reached 12.3 nmol/mg protein. During lithotrophic growth, the maximum values of the ATP concentration in the cells of S. thermotolerans Kr1 and A. tolerans K1 were 0.3 and
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I A Tsaplina, T I Bogdanova, Tat'yana P Tourova, E. N. Krasil’nikova, Marya A Egorova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA–DNA hybridization data and phenotypic properties revealed that ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99·6 %), Alicyclobacillus sp. 5C (98·9 %) and Alicyclobacillus sp. CLG (98·6 %) and bacterium GSM (99·1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92·1–94·6 %, and for S. thermosulfidooxidans VKM B-1269T the value was 87·7 %. Sulfobacillus disulfidooxidans SD-11T was also phylogenetically related to strain K1 (92·6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11T (ω-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11T to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11T from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48·7±0·6 mol%; that of strain SD-11T was 53±1 mol%. DNA–DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11T, and even lower relatedness (3–5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009T, DSM 446T. DNA reassociation of strains K1 and SD-11T with Alicyclobacillus cycloheptanicus DSM 4006T gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11T, Alicyclobacillus tolerans sp. nov. (type strain, K1T=VKM B-2304T=DSM 16297T) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11T=ATCC 51911T=DSM 12064T) are proposed.
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I A Tsaplina, Tat'yana I Bogdanova, Tat'yana P Tourova, Marya A Egorova, Elena N Krasil'nikova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA-DNA hybridization data and phenotypic properties revealed that 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99.6 %), Alicyclobacillus sp. 5C (98.9 %) and Alicyclobacillus sp. CLG (98.6 %) and bacterium GSM (99.1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92.1-94.6 %, and for S. thermosulfidooxidans VKM B-1269(T) the value was 87.7 %. Sulfobacillus disulfidooxidans SD-11(T) was also phylogenetically related to strain K1 (92.6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11(T) (omega-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11(T) to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11(T) from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48.7+/-0.6 mol%; that of strain SD-11(T) was 53+/-1 mol%. DNA-DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11(T), and even lower relatedness (3-5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009(T), DSM 446(T). DNA reassociation of strains K1 and SD-11(T) with Alicyclobacillus cycloheptanicus DSM 4006(T) gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11(T), Alicyclobacillus tolerans sp. nov. (type strain, K1(T)=VKM B-2304(T)=DSM 16297(T)) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11(T)=ATCC 51911(T)=DSM 12064(T)) are proposed.
Grigorii I Karavaiko - One of the best experts on this subject based on the ideXlab platform.
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The dependence of intracellular ATP level on the nutrition mode of the acidophilic bacteria Sulfobacillus thermotolerans and Alicyclobacillus tolerans
Microbiology, 2007Co-Authors: I A Tsaplina, T I Bogdanova, E. N. Krasil’nikova, Leonid M Zakharchuk, A. E. Zhuravleva, A. D. Ismailov, Grigorii I KaravaikoAbstract:The dynamics of the ATP pool in the aerobic spore-forming acidothermophilic mixotrophic bacteria Sulfobacillus thermotolerans Kr1^T and Alicyclobacillus tolerans K1^T were studied in the course of their chemolithoheterotrophic, chemoorganoheterotrophic, and chemolithoautotrophic growth. It was established that, during mixotrophic growth, the maximum ATP concentrations in the cells of S. thermotolerans Kr1 and A. tolerans K1 were 3.8 and 0.6 nmol/mg protein, respectively. The ATP concentrations in sulfobacilli and alicyclobacilli during organotrophic growth were 2.2 and 3.1 nmol/mg protein, respectively. In the cells of the obligately heterotrophic bacterium Alicyclobacillus cycloheptanicus 4006^T, the maximum ATP concentration was several times higher and reached 12.3 nmol/mg protein. During lithotrophic growth, the maximum values of the ATP concentration in the cells of S. thermotolerans Kr1 and A. tolerans K1 were 0.3 and
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I A Tsaplina, T I Bogdanova, Tat'yana P Tourova, E. N. Krasil’nikova, Marya A Egorova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA–DNA hybridization data and phenotypic properties revealed that ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99·6 %), Alicyclobacillus sp. 5C (98·9 %) and Alicyclobacillus sp. CLG (98·6 %) and bacterium GSM (99·1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92·1–94·6 %, and for S. thermosulfidooxidans VKM B-1269T the value was 87·7 %. Sulfobacillus disulfidooxidans SD-11T was also phylogenetically related to strain K1 (92·6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11T (ω-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11T to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11T from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48·7±0·6 mol%; that of strain SD-11T was 53±1 mol%. DNA–DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11T, and even lower relatedness (3–5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009T, DSM 446T. DNA reassociation of strains K1 and SD-11T with Alicyclobacillus cycloheptanicus DSM 4006T gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11T, Alicyclobacillus tolerans sp. nov. (type strain, K1T=VKM B-2304T=DSM 16297T) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11T=ATCC 51911T=DSM 12064T) are proposed.
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I A Tsaplina, Tat'yana I Bogdanova, Tat'yana P Tourova, Marya A Egorova, Elena N Krasil'nikova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA-DNA hybridization data and phenotypic properties revealed that 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99.6 %), Alicyclobacillus sp. 5C (98.9 %) and Alicyclobacillus sp. CLG (98.6 %) and bacterium GSM (99.1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92.1-94.6 %, and for S. thermosulfidooxidans VKM B-1269(T) the value was 87.7 %. Sulfobacillus disulfidooxidans SD-11(T) was also phylogenetically related to strain K1 (92.6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11(T) (omega-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11(T) to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11(T) from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48.7+/-0.6 mol%; that of strain SD-11(T) was 53+/-1 mol%. DNA-DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11(T), and even lower relatedness (3-5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009(T), DSM 446(T). DNA reassociation of strains K1 and SD-11(T) with Alicyclobacillus cycloheptanicus DSM 4006(T) gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11(T), Alicyclobacillus tolerans sp. nov. (type strain, K1(T)=VKM B-2304(T)=DSM 16297(T)) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11(T)=ATCC 51911(T)=DSM 12064(T)) are proposed.
Bin Yao - One of the best experts on this subject based on the ideXlab platform.
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identification of an acidic α amylase from Alicyclobacillus sp a4 and assessment of its application in the starch industry
Food Chemistry, 2012Co-Authors: Yingguo Bai, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Kun Meng, Peilong Yang, Yukun Feng, Wei Zhang, Bin YaoAbstract:Abstract An acidic α-amylase was purified from thermoacidophilic Alicyclobacillus sp. A4 by ion exchange chromatography with 22% recovery, and showed a molecular mass of 64 kDa by SDS–PAGE. Its amino acid sequence was determined by sequencing three internal peptides and the complete genome of strain A4, and shared highest identity (64%) with Alicyclobacillus acidocaldarius α-amylase. Compared with other reported α-amylases, the purified enzyme had some distinct characteristics. The optimal activity was found to occur at 75 °C and pH 4.2, similar to the glucamylase widely used in the starch industry. The enzyme was Ca2+ independent, and had strong ability to digest raw starch (96.71%) with commercial glucamylase in one step. These properties of the purified enzyme make up the deficiency of the commercial α-amylases currently used and avoid repeated adjustment of pH and temperature in double-enzymatic sugar-making process. The purified enzyme will be commercially valuable in the starch industry.
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Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
Microbial cell factories, 2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the nutrient bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4F, both of which affected the expression efficiency. The physical properties of various forms of CelA4 as they affected enzymatic activity were characterized.
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from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background: Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the nutrient bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4 F , both of which affected the expression efficiency. The physical
I A Tsaplina - One of the best experts on this subject based on the ideXlab platform.
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The dependence of intracellular ATP level on the nutrition mode of the acidophilic bacteria Sulfobacillus thermotolerans and Alicyclobacillus tolerans
Microbiology, 2007Co-Authors: I A Tsaplina, T I Bogdanova, E. N. Krasil’nikova, Leonid M Zakharchuk, A. E. Zhuravleva, A. D. Ismailov, Grigorii I KaravaikoAbstract:The dynamics of the ATP pool in the aerobic spore-forming acidothermophilic mixotrophic bacteria Sulfobacillus thermotolerans Kr1^T and Alicyclobacillus tolerans K1^T were studied in the course of their chemolithoheterotrophic, chemoorganoheterotrophic, and chemolithoautotrophic growth. It was established that, during mixotrophic growth, the maximum ATP concentrations in the cells of S. thermotolerans Kr1 and A. tolerans K1 were 3.8 and 0.6 nmol/mg protein, respectively. The ATP concentrations in sulfobacilli and alicyclobacilli during organotrophic growth were 2.2 and 3.1 nmol/mg protein, respectively. In the cells of the obligately heterotrophic bacterium Alicyclobacillus cycloheptanicus 4006^T, the maximum ATP concentration was several times higher and reached 12.3 nmol/mg protein. During lithotrophic growth, the maximum values of the ATP concentration in the cells of S. thermotolerans Kr1 and A. tolerans K1 were 0.3 and
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I A Tsaplina, T I Bogdanova, Tat'yana P Tourova, E. N. Krasil’nikova, Marya A Egorova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA–DNA hybridization data and phenotypic properties revealed that ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99·6 %), Alicyclobacillus sp. 5C (98·9 %) and Alicyclobacillus sp. CLG (98·6 %) and bacterium GSM (99·1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92·1–94·6 %, and for S. thermosulfidooxidans VKM B-1269T the value was 87·7 %. Sulfobacillus disulfidooxidans SD-11T was also phylogenetically related to strain K1 (92·6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11T (ω-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11T to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11T from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48·7±0·6 mol%; that of strain SD-11T was 53±1 mol%. DNA–DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11T, and even lower relatedness (3–5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009T, DSM 446T. DNA reassociation of strains K1 and SD-11T with Alicyclobacillus cycloheptanicus DSM 4006T gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11T, Alicyclobacillus tolerans sp. nov. (type strain, K1T=VKM B-2304T=DSM 16297T) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11T=ATCC 51911T=DSM 12064T) are proposed.
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Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended descri
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Grigorii I Karavaiko, Tamara F. Kondrat'eva, I A Tsaplina, Tat'yana I Bogdanova, Tat'yana P Tourova, Marya A Egorova, Elena N Krasil'nikova, Leonid M ZakharchukAbstract:Comparative analysis of 16S rRNA gene sequences, DNA-DNA hybridization data and phenotypic properties revealed that 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99.6 %), Alicyclobacillus sp. 5C (98.9 %) and Alicyclobacillus sp. CLG (98.6 %) and bacterium GSM (99.1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92.1-94.6 %, and for S. thermosulfidooxidans VKM B-1269(T) the value was 87.7 %. Sulfobacillus disulfidooxidans SD-11(T) was also phylogenetically related to strain K1 (92.6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11(T) (omega-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11(T) to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11(T) from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48.7+/-0.6 mol%; that of strain SD-11(T) was 53+/-1 mol%. DNA-DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11(T), and even lower relatedness (3-5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009(T), DSM 446(T). DNA reassociation of strains K1 and SD-11(T) with Alicyclobacillus cycloheptanicus DSM 4006(T) gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11(T), Alicyclobacillus tolerans sp. nov. (type strain, K1(T)=VKM B-2304(T)=DSM 16297(T)) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11(T)=ATCC 51911(T)=DSM 12064(T)) are proposed.
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determination of the phylogenetic position of sulfobacillus thermosulfidooxidans on the basis of analysis of the 5s and 16s ribosomal rna
Microbiology, 1995Co-Authors: T P Turova, A B Poltoraus, I A Lebedeva, E S Bulygina, I A Tsaplina, T I Bogdanova, G I KaravaĭkoAbstract:: The nucleotide sequence of the 16S rRNA gene of the type strain Sulfobacillus thermosulfidooxidans VKM-1269 was determined. This microorganism belongs to the group of thermophilic acidophilic chemolithotrophic bacteria capable of utilizing elemental sulfur and iron ions as the sole energy sources. The phylogenetic position of the bacterium studied among Gram-positive bacteria was estimated by a comparative analysis of 16S rRNA nucleotide sequences. It was shown that Sulfobacillus forms a coherent group with species of a new genus Alicyclobacillus. The Sulfobacillus-Alicyclobacillus group is suggested to represent a separate evolutionary branch within the clostridial subdivision of Gram-positive bacteria.
Tianli Yue - One of the best experts on this subject based on the ideXlab platform.
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targeting the vanillic acid decarboxylase gene for Alicyclobacillus acidoterrestris quantification and guaiacol assessment in apple juices using real time pcr
International Journal of Food Microbiology, 2021Co-Authors: Zhouli Wang, Yahong Yuan, Tianli Yue, Yanchen Zhang, Zhenpeng Gao, Rui CaiAbstract:Abstract Alicyclobacillus spp. has recently received much attention due to its implication in the spoilage of pasteurized fruit juices, which is characterized by the formation of guaiacol. Previous researches indicate that not all Alicyclobacillus spp. are able to produce guaiacol. The aim of this study was to identify possible differences in the vanillic acid decarboxylase gene involved in guaiacol biosynthesis and then develop specific detection methods for guaiacol producing Alicyclobacillus. Agarose gel electrophoresis results showed that the partial vdcC gene was present in all the guaiacol producing Alicyclobacillus, but absent in non-guaicaol producing strains apart from A. fastidiosus DSM 17978. On the basis of the vdcC gene sequence, a primer pair specific to A. acidoterrestris was designed; then a SYBR Green I real time PCR was established for the direct quantification of A. acidoterrestris in apple juice, and the detection limit was 2.6 × 101 CFU/mL. The developed real time PCR system was used to detect A. acidoterrestris in 36 artificially contaminated apple juice samples and guaiacol production in the sample was also analyzed by GC–MS. The Gompertz model was employed to describe the relationship between A. acidoterrestris cell concentration and guaiacol content, and the value of R2 was 0.854. This work provides an alternative to conventional methods of guaiacol quantification and A. acidoterrestris detection and could be very useful for the early recognition of A. acidoterrestris contamination in fruit juices.
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Bacteriocin assisted food functional membrane for simultaneous exclusion and inactivation of Alicyclobacillus acidoterrestris in apple juice
Journal of Membrane Science, 2021Co-Authors: Jinye Zhang, Tianli Yue, Wenjing Wang, Jianfei Pei, Jiawei Zhang, Wirote YouravongAbstract:Abstract Alicyclobacillus acidoterrestris is a thermophilic and acidophilic bacilli species which causes a significant decay in quality of apple juice and it cannot be efficiently treated by conventional sterilization procedures in food processing. In order to efficiently remove and inactivate Alicyclobacillus acidoterrestris in apple juice, a food functional microfiltration membrane was fabricated by a multi-stage modification including polydopamine coating and nisin grafting. Attenuated total reflectance-fourier transform infrared spectrometer and scanning electron microscopy images confirmed the success of immobilizing polydopamine and nisin microspheres onto the polyvinylidene difluoride membrane surface. In comparison with pristine polyvinylidene difluoride membrane, the modified membrane showed promoted performance in terms of hydrophilicity, water flux and the rejection of Alicyclobacillus acidoterrestris. A simultaneous exclusion and inactivation of Alicyclobacillus acidoterrestris in apple juice was achieved by modified microfiltration membrane. The modified membrane also exhibited reliable stability under different membrane cleaning procedures. The present study provides an option for the development of tailored food functional membrane for specific applications in food processing.
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synthesis of multifunctional fluorescent magnetic nanoparticles for the detection of Alicyclobacillus spp in apple juice
Food Research International, 2018Co-Authors: Zhouli Wang, Yahong Yuan, Rui Cai, Yuejuan Zhao, Tianli YueAbstract:Abstract An approach based on multifunctional fluorescent magnetic nanoparticles was proposed for the enrichment and identification of Alicyclobacillus spp. in apple juice simultaneously. The prepared Fe3O4 magnetic particles (MNPs) were modified by the sol-gel process and a silica shell was formed to improve the reactivity, and then the obtained MNPs@SiO2-SH nanoparticles were conjugated with Thioglycolic acid functionalized CdTe/CdS QDs via thiols chemistry. The characteristic evaluation results indicated that the MNPs-QD nanocomposites exhibited good magnetic properties and optical characterization. The polyclonal anti-Alicyclobacillus IgG antibody was immobilized onto the surface of MNPs-QD materials via esterification reactions. The maximum antibody immobilization capacity was 119.62 μg/mg and the adsorption reaction could be accomplished in 60 min. The adsorption process could be represented by Langmuir model and pseudo-second order kinetics equation, respectively. Based on the high immunocapture efficiency and sensitive fluorescence characteristics, the obtained MNPs-QDs-antibody conjugates could be applied to recognize the contamination of Alicyclobacillus spp. and a quantitative analysis method was established for target cells detection. The minimum quantitative limit was 104 CFU (colony forming unit)/mL and the testing process could be completed in 90 min. The results indicated that the MNPs-QDs-antibody conjugates can be successfully applied for immunocapture and detection of Alicyclobacillus spp. in apple juice. That is to say, the developed MNPs-QDs-antibody conjugates have exhibit more attractive and great potential for the immunocapture and recognition of target bacteria, fully demonstrated a new method for enrichment and rapid detection of Alicyclobacillus spp. in fruit juices.
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Effective Adsorption of Patulin from Apple Juice by Using Non-Cytotoxic Heat-Inactivated Cells and Spores of Alicyclobacillus Strains
MDPI AG, 2018Co-Authors: Marina Sajid, Yahong Yuan, Chen Niu, Sajid Mehmood, Tianli YueAbstract:Patulin (PAT) is a major threat to many food products, especially apple and apple products, causing human health risks and economic losses. The aim of this study was to remove PAT from apple juice by using the heat-inactivated (HI) cells and spores of seven Alicyclobacillus strains under controlled conditions. The HI cells and spores of seven strains adsorbed PAT effectively, and the HI cells and spores of Alicyclobacillus acidocaldarius DSM 451 (A51) showed maximum PAT adsorption capacity of up to 12.621 μg/g by HI cells and 11.751 μg/g by HI spores at 30 °C and pH 4.0 for 24 h. Moreover, the PAT adsorption process followed the pseudo-first order kinetic model and the Freundlich isotherm model; thermodynamic parameters revealed that PAT adsorption is a spontaneous exothermic physisorption process. The results also indicated that PAT adsorption is strain-specific. The HI cells and spores of Alicyclobacillus strains are non-cytotoxic, and the bioadsorption of PAT did not affect the quality of the juice. Furthermore, the cell wall surface plays an important role in the adsorption process
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detection of Alicyclobacillus spp in fruit juice by combination of immunomagnetic separation and a sybr green i real time pcr assay
PLOS ONE, 2015Co-Authors: Rui Cai, Yahong Yuan, Zhouli Wang, Bin Liu, Ling Wang, Tianli YueAbstract:An approach based on immunomagnetic separation (IMS) and SYBR Green I real-time PCR (real-time PCR) with species-specific primers and melting curve analysis was proposed as a rapid and effective method for detecting Alicyclobacillus spp. in fruit juices. Specific primers targeting the 16S rDNA sequences of Alicyclobacillus spp. were designed and then confirmed by the amplification of DNA extracted from standard strains and isolates. Spiked samples containing known amounts of target bacteria were used to obtain standard curves; the correlation coefficient was greater than 0.986 and the real-time PCR amplification efficiencies were 98.9%- 101.8%. The detection limit of the testing system was 2.8×101 CFU/mL. The coefficient of variation for intra-assay and inter-assay variability were all within the acceptable limit of 5%. Besides, the performance of the IMS-real-time PCR assay was further investigated by detecting naturally contaminated kiwi fruit juice; the sensitivity, specificity and accuracy were 91.7%, 95.9% and 95.3%, respectively. The established IMS-real-time PCR procedure provides a new method for identification and quantitative detection of Alicyclobacillus spp. in fruit juice.
