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En Tao Wang - One of the best experts on this subject based on the ideXlab platform.
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two distinctive rhizobium genospecies nodulating vicia villosa roth in Alkaline Soils of northwest china
Plant and Soil, 2020Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Yimin Shang, Chunzeng Liu, Shanshan Peng, Chen Guo, Pei Hou, Brigitte BrunelAbstract:To estimate the relative abundance, diversity and distribution of rhizobia associated with Vicia villosa Roth in Soils of Northwest China. Rhizobia were trapped with V. villosa Roth plants from Soils at seven sites and identified into genotypes by PCR-RFLP of IGS genes. They were further characterized by phylogenetic analyses of 16S rRNA, housekeeping genes (atpD, recA, glnII) and symbiotic genes (nodC). Soil physicochemical characteristics were recorded and Canonical Correlation Analysis was performed to correlate soil features and the distribution of IGS genotypes. 253 rhizobial strains were obtained from Soils with pH 7.1–7.9. They were discriminated into 27 IGS types and further affiliated by phylogenetic analyses of 16S rRNA and multilocus sequence analysis (MLSA) into two clusters in Rhizobium. One corresponds to Rhizobium sophorae covering 228 strains in 25 IGS types and the other includes a novel genospecies containing two IGS types only detected in Shanxi Province, with higher soil pH and intermediate available phosphate and potassium contents. Representative strains of the novel genospecies show a specific phylogenetic nodC lineage, which may reflect differences in symbiosis properties. Furthermore, the Shanxi site was the less IGS diversified, based on Shannon diversity indices varying from 0.96 to 2.19 along the sampled area. As the first study on diversity of rhizobia nodulating V. villosa Roth in China, R. sophorae and a novel Rhizobium genospecies with specific symbiotic genes were identified; and biogeographic patterns were found, mainly directed by soil pH, organic matter and soil salinity, including available phosphorus salts.
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genetic diversity and community structure of rhizobia nodulating sesbania cannabina in saline Alkaline Soils
Systematic and Applied Microbiology, 2016Co-Authors: Yajing Liu, En Tao Wang, Chenggang Ren, Wei Liu, Nan Jiang, Xiaoli Zhang, Zhi Hong XieAbstract:Sesbania cannabina is a plant that grows naturally along the seashores in Rudong County, China (RDC) and it has been introduced into the Yellow River Delta (YRD) as a pioneer plant to improve the saline–Alkaline Soils. In order to investigate the diversity of S. cannabina rhizobia in these Soils, a total of 198 rhizobial isolates were characterized and phylogenetic trees were constructed based on data from multilocus sequence analysis (MLSA) of the housekeeping genes recA, atpD and glnII, as well as 16S rRNA. Symbiotic features were also studied by establishing the phylogeny of the symbiotic genes nodA and nifH, and by performing nodulation assays. The isolates had highly conserved symbiotic genes and were classified into nine genospecies belonging to the genera Ensifer, Agrobacterium, Neorhizobium and Rhizobium. A unique community structure was detected in the rhizobia associated with S. cannabina in the saline–Alkaline Soils that was characterized by five novel genospecies and four defined species. In addition, Ensifer sp. I was the predominant rhizobia in YRD, whereas Ensifer meliloti and Neorhizobium huautlense were the dominant species in RDC. Therefore, the study demonstrated for the first time that this plant strongly selected the symbiotic gene background but not the genomic background of its microsymbionts. In addition, biogeographic patterns existed in the rhizobial populations associated with S. cannabina, which were mainly correlated with pH and salinity, as well as the mineral nutrient contents. This study provided novel information concerning the interaction between soil conditions, host plant and rhizobia, in addition to revealing the diversity of S. cannabina rhizobia in saline–Alkaline Soils.
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association of white clover trifolium repens l with rhizobia of sv trifolii belonging to three genomic species in Alkaline Soils in north and east china
Plant and Soil, 2016Co-Authors: Jun Jie Zhang, Wen-feng Chen, Xiao Yan Jing, Philippe De Lajudie, Raghvendra Singh, En Tao WangAbstract:Rhizobia associated with white clover (Trifolium repens) grown in Alkaline Soils of China have never been investigated. This is the first survey to report of their genetic and biogeographical diversity. Nodule bacteria were isolated from white clover grown in Alkaline Soils (pH 8.18–8.99) in North and East China and were characterized by multilocus sequence analysis (MLSA) of the housekeeping genes (atpD, recA, and glnII), and phylogenies of 16S rRNA gene and symbiotic genes nodC and nifH. The biogeographic distribution of rhizobial species was analyzed in relation to the soil factors. A total of 83 new strains could be affiliated to Rhizobium that shared 100 % sequence similarity of 16S rRNA gene with R. leguminosarum, R. acidisoli, R. anhuiense, R. indigoferae, R. sophorae, and R. laguerreae. Three genospecies were further distinguished based on the housekeeping gene analysis among these new strains: R. anhuiense, R. leguminosarum, and a hypothetical novel Rhizobium genospecies. Highly conserved symbiotic genes corresponding to those of symbiovar trifolii in R. leguminosarum were observed among all the new strains. Unique rhizobial communities associated with white clover were detected in the tested Alkaline Soils, and soil characteristics such as pH and nutrient levels were estimated as the determinant factors. White clover established symbiosis with three Rhizobium genospecies harboring similar symbiotic genes in Alkaline Soils in China. Biogeographic pattern exists in clover rhizobia that was determined by the soil pH and nutrient levels.
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microsymbionts of phaseolus vulgaris in acid and Alkaline Soils of mexico
Systematic and Applied Microbiology, 2014Co-Authors: Myrthala M Verasteguivaldes, Yujing Zhang, Flor N Riveraorduna, Haiping Cheng, Xing Hua Sui, En Tao WangAbstract:In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and Alkaline Soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid Soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from Alkaline Soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred Alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.
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genotypic alteration and competitive nodulation of mesorhizobium muleiense against exotic chickpea rhizobia in Alkaline Soils
Systematic and Applied Microbiology, 2014Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Tao Yu, Wen Xin ChenAbstract:Abstract Mesorhizobium muleiense , Mesorhizobium mediterraneum and Mesorhizobium ciceri are chickpea ( Cicer arietinum L.) rhizobia that share a high similarity of the symbiotic genes nodC and nifH , but they have different geographic distributions. M. muleiense has been isolated and found only in Alkaline Soils of Xinjiang, China, whereas the other two strains have been found in the Mediterranean and India. To investigate the species stability of M. muleiense during natural evolution and its capability of competitive nodulation against the other two exotic species, re-sampling of nodules in the field and competition experiments between the three species were conducted. The results showed that the predominant microsymbiont associated with chickpea grown in Xinjiang was still M. muleiense , but the predominant genotypes of M. muleiense had changed significantly during the four years since a previous survey. The data also showed that M. mediterraneum and M. ciceri were more competitive than the residential strain of M. muleiense CCBAU 83963 T in sterilized vermiculite or Soils from Xinjiang. However, in non-sterilized Soils, M. muleiense was the predominant nodule occupier. These results indicated that natural or adapting evolution of M. muleiense was occurring in fields subjected to changing environmental factors. In addition, the biogeography and symbiotic associations of rhizobia with their host legumes were also influenced by biological factors in the soil, such as indigenous rhizobia and other organisms.
Jun Jie Zhang - One of the best experts on this subject based on the ideXlab platform.
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two distinctive rhizobium genospecies nodulating vicia villosa roth in Alkaline Soils of northwest china
Plant and Soil, 2020Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Yimin Shang, Chunzeng Liu, Shanshan Peng, Chen Guo, Pei Hou, Brigitte BrunelAbstract:To estimate the relative abundance, diversity and distribution of rhizobia associated with Vicia villosa Roth in Soils of Northwest China. Rhizobia were trapped with V. villosa Roth plants from Soils at seven sites and identified into genotypes by PCR-RFLP of IGS genes. They were further characterized by phylogenetic analyses of 16S rRNA, housekeeping genes (atpD, recA, glnII) and symbiotic genes (nodC). Soil physicochemical characteristics were recorded and Canonical Correlation Analysis was performed to correlate soil features and the distribution of IGS genotypes. 253 rhizobial strains were obtained from Soils with pH 7.1–7.9. They were discriminated into 27 IGS types and further affiliated by phylogenetic analyses of 16S rRNA and multilocus sequence analysis (MLSA) into two clusters in Rhizobium. One corresponds to Rhizobium sophorae covering 228 strains in 25 IGS types and the other includes a novel genospecies containing two IGS types only detected in Shanxi Province, with higher soil pH and intermediate available phosphate and potassium contents. Representative strains of the novel genospecies show a specific phylogenetic nodC lineage, which may reflect differences in symbiosis properties. Furthermore, the Shanxi site was the less IGS diversified, based on Shannon diversity indices varying from 0.96 to 2.19 along the sampled area. As the first study on diversity of rhizobia nodulating V. villosa Roth in China, R. sophorae and a novel Rhizobium genospecies with specific symbiotic genes were identified; and biogeographic patterns were found, mainly directed by soil pH, organic matter and soil salinity, including available phosphorus salts.
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association of white clover trifolium repens l with rhizobia of sv trifolii belonging to three genomic species in Alkaline Soils in north and east china
Plant and Soil, 2016Co-Authors: Jun Jie Zhang, Wen-feng Chen, Xiao Yan Jing, Philippe De Lajudie, Raghvendra Singh, En Tao WangAbstract:Rhizobia associated with white clover (Trifolium repens) grown in Alkaline Soils of China have never been investigated. This is the first survey to report of their genetic and biogeographical diversity. Nodule bacteria were isolated from white clover grown in Alkaline Soils (pH 8.18–8.99) in North and East China and were characterized by multilocus sequence analysis (MLSA) of the housekeeping genes (atpD, recA, and glnII), and phylogenies of 16S rRNA gene and symbiotic genes nodC and nifH. The biogeographic distribution of rhizobial species was analyzed in relation to the soil factors. A total of 83 new strains could be affiliated to Rhizobium that shared 100 % sequence similarity of 16S rRNA gene with R. leguminosarum, R. acidisoli, R. anhuiense, R. indigoferae, R. sophorae, and R. laguerreae. Three genospecies were further distinguished based on the housekeeping gene analysis among these new strains: R. anhuiense, R. leguminosarum, and a hypothetical novel Rhizobium genospecies. Highly conserved symbiotic genes corresponding to those of symbiovar trifolii in R. leguminosarum were observed among all the new strains. Unique rhizobial communities associated with white clover were detected in the tested Alkaline Soils, and soil characteristics such as pH and nutrient levels were estimated as the determinant factors. White clover established symbiosis with three Rhizobium genospecies harboring similar symbiotic genes in Alkaline Soils in China. Biogeographic pattern exists in clover rhizobia that was determined by the soil pH and nutrient levels.
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genotypic alteration and competitive nodulation of mesorhizobium muleiense against exotic chickpea rhizobia in Alkaline Soils
Systematic and Applied Microbiology, 2014Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Tao Yu, Wen Xin ChenAbstract:Abstract Mesorhizobium muleiense , Mesorhizobium mediterraneum and Mesorhizobium ciceri are chickpea ( Cicer arietinum L.) rhizobia that share a high similarity of the symbiotic genes nodC and nifH , but they have different geographic distributions. M. muleiense has been isolated and found only in Alkaline Soils of Xinjiang, China, whereas the other two strains have been found in the Mediterranean and India. To investigate the species stability of M. muleiense during natural evolution and its capability of competitive nodulation against the other two exotic species, re-sampling of nodules in the field and competition experiments between the three species were conducted. The results showed that the predominant microsymbiont associated with chickpea grown in Xinjiang was still M. muleiense , but the predominant genotypes of M. muleiense had changed significantly during the four years since a previous survey. The data also showed that M. mediterraneum and M. ciceri were more competitive than the residential strain of M. muleiense CCBAU 83963 T in sterilized vermiculite or Soils from Xinjiang. However, in non-sterilized Soils, M. muleiense was the predominant nodule occupier. These results indicated that natural or adapting evolution of M. muleiense was occurring in fields subjected to changing environmental factors. In addition, the biogeography and symbiotic associations of rhizobia with their host legumes were also influenced by biological factors in the soil, such as indigenous rhizobia and other organisms.
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distinctive mesorhizobium populations associated with cicer arietinum l in Alkaline Soils of xinjiang china
Plant and Soil, 2012Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Kai Lou, Xiang Jin, Pei Hong Mao, Chang Fu Tian, Xin Hua Sui, Wen Xin ChenAbstract:Rhizobia associated with chickpea in the main chickpea production zone of Xinjiang, China have never been investigated. Here, we present the first systematic investigation of these rhizobia’s genetic diversity and symbiotic interactions with their host plant. Ninety-five isolates obtained from chickpea nodules in eight Alkaline-saline (pH 8.24–8.45) sites in Xinjiang were characterized by nodulation test, symbiotic gene analysis, PCR-based restriction fragment length polymorphism (RFLP) of the 16S rRNA gene and 16S–23S rRNA intergenic spacer (IGS), BOX-PCR, phylogenies of 16S rRNA and housekeeping genes (atpD, recA and glnII), multilocus sequence analysis (MLSA) and DNA–DNA hybridization. All 95 isolates were identified within the genus of Mesorhizobium. Similarities less than 96.5% in MLSA and DNA–DNA hybridization values ( 98%) of symbiotic genes (nodC and nifH) with those of the well studied chickpea microsymbioints Mesorhizobium ciceri and Mesorhizobium mediterraneum were found. Chickpea rhizobia in Alkaline-saline Soils of Xinjiang, China, form a population distinct from the defined Mesorhizobium species. All these chickpea rhizobia in Xinjiang harbored symbiotic genes highly similar to the type strains of two well-studied chickpea rhizobia, M. ciceri and M. mediterraneum, evidencing the possible lateral transfer of symbiotic genes among these different rhizobial species. On the other hand, chickpea may strongly select rhizobia with a unique symbiotic gene background.
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distinctive mesorhizobium populations associated with cicer arietinum l in Alkaline Soils of xinjiang china
Plant and Soil, 2012Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Chang Fu Tian, Wen Xin ChenAbstract:Background and aims Rhizobia associated with chickpea in the main chickpea production zone of Xinjiang, China have never been investigated. Here, we present the first systematic investigation of these rhizobia’s genetic diversity and symbiotic interactions with their host plant.
Brigitte Brunel - One of the best experts on this subject based on the ideXlab platform.
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two distinctive rhizobium genospecies nodulating vicia villosa roth in Alkaline Soils of northwest china
Plant and Soil, 2020Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Yimin Shang, Chunzeng Liu, Shanshan Peng, Chen Guo, Pei Hou, Brigitte BrunelAbstract:To estimate the relative abundance, diversity and distribution of rhizobia associated with Vicia villosa Roth in Soils of Northwest China. Rhizobia were trapped with V. villosa Roth plants from Soils at seven sites and identified into genotypes by PCR-RFLP of IGS genes. They were further characterized by phylogenetic analyses of 16S rRNA, housekeeping genes (atpD, recA, glnII) and symbiotic genes (nodC). Soil physicochemical characteristics were recorded and Canonical Correlation Analysis was performed to correlate soil features and the distribution of IGS genotypes. 253 rhizobial strains were obtained from Soils with pH 7.1–7.9. They were discriminated into 27 IGS types and further affiliated by phylogenetic analyses of 16S rRNA and multilocus sequence analysis (MLSA) into two clusters in Rhizobium. One corresponds to Rhizobium sophorae covering 228 strains in 25 IGS types and the other includes a novel genospecies containing two IGS types only detected in Shanxi Province, with higher soil pH and intermediate available phosphate and potassium contents. Representative strains of the novel genospecies show a specific phylogenetic nodC lineage, which may reflect differences in symbiosis properties. Furthermore, the Shanxi site was the less IGS diversified, based on Shannon diversity indices varying from 0.96 to 2.19 along the sampled area. As the first study on diversity of rhizobia nodulating V. villosa Roth in China, R. sophorae and a novel Rhizobium genospecies with specific symbiotic genes were identified; and biogeographic patterns were found, mainly directed by soil pH, organic matter and soil salinity, including available phosphorus salts.
Wen-feng Chen - One of the best experts on this subject based on the ideXlab platform.
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two distinctive rhizobium genospecies nodulating vicia villosa roth in Alkaline Soils of northwest china
Plant and Soil, 2020Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Yimin Shang, Chunzeng Liu, Shanshan Peng, Chen Guo, Pei Hou, Brigitte BrunelAbstract:To estimate the relative abundance, diversity and distribution of rhizobia associated with Vicia villosa Roth in Soils of Northwest China. Rhizobia were trapped with V. villosa Roth plants from Soils at seven sites and identified into genotypes by PCR-RFLP of IGS genes. They were further characterized by phylogenetic analyses of 16S rRNA, housekeeping genes (atpD, recA, glnII) and symbiotic genes (nodC). Soil physicochemical characteristics were recorded and Canonical Correlation Analysis was performed to correlate soil features and the distribution of IGS genotypes. 253 rhizobial strains were obtained from Soils with pH 7.1–7.9. They were discriminated into 27 IGS types and further affiliated by phylogenetic analyses of 16S rRNA and multilocus sequence analysis (MLSA) into two clusters in Rhizobium. One corresponds to Rhizobium sophorae covering 228 strains in 25 IGS types and the other includes a novel genospecies containing two IGS types only detected in Shanxi Province, with higher soil pH and intermediate available phosphate and potassium contents. Representative strains of the novel genospecies show a specific phylogenetic nodC lineage, which may reflect differences in symbiosis properties. Furthermore, the Shanxi site was the less IGS diversified, based on Shannon diversity indices varying from 0.96 to 2.19 along the sampled area. As the first study on diversity of rhizobia nodulating V. villosa Roth in China, R. sophorae and a novel Rhizobium genospecies with specific symbiotic genes were identified; and biogeographic patterns were found, mainly directed by soil pH, organic matter and soil salinity, including available phosphorus salts.
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association of white clover trifolium repens l with rhizobia of sv trifolii belonging to three genomic species in Alkaline Soils in north and east china
Plant and Soil, 2016Co-Authors: Jun Jie Zhang, Wen-feng Chen, Xiao Yan Jing, Philippe De Lajudie, Raghvendra Singh, En Tao WangAbstract:Rhizobia associated with white clover (Trifolium repens) grown in Alkaline Soils of China have never been investigated. This is the first survey to report of their genetic and biogeographical diversity. Nodule bacteria were isolated from white clover grown in Alkaline Soils (pH 8.18–8.99) in North and East China and were characterized by multilocus sequence analysis (MLSA) of the housekeeping genes (atpD, recA, and glnII), and phylogenies of 16S rRNA gene and symbiotic genes nodC and nifH. The biogeographic distribution of rhizobial species was analyzed in relation to the soil factors. A total of 83 new strains could be affiliated to Rhizobium that shared 100 % sequence similarity of 16S rRNA gene with R. leguminosarum, R. acidisoli, R. anhuiense, R. indigoferae, R. sophorae, and R. laguerreae. Three genospecies were further distinguished based on the housekeeping gene analysis among these new strains: R. anhuiense, R. leguminosarum, and a hypothetical novel Rhizobium genospecies. Highly conserved symbiotic genes corresponding to those of symbiovar trifolii in R. leguminosarum were observed among all the new strains. Unique rhizobial communities associated with white clover were detected in the tested Alkaline Soils, and soil characteristics such as pH and nutrient levels were estimated as the determinant factors. White clover established symbiosis with three Rhizobium genospecies harboring similar symbiotic genes in Alkaline Soils in China. Biogeographic pattern exists in clover rhizobia that was determined by the soil pH and nutrient levels.
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genotypic alteration and competitive nodulation of mesorhizobium muleiense against exotic chickpea rhizobia in Alkaline Soils
Systematic and Applied Microbiology, 2014Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Tao Yu, Wen Xin ChenAbstract:Abstract Mesorhizobium muleiense , Mesorhizobium mediterraneum and Mesorhizobium ciceri are chickpea ( Cicer arietinum L.) rhizobia that share a high similarity of the symbiotic genes nodC and nifH , but they have different geographic distributions. M. muleiense has been isolated and found only in Alkaline Soils of Xinjiang, China, whereas the other two strains have been found in the Mediterranean and India. To investigate the species stability of M. muleiense during natural evolution and its capability of competitive nodulation against the other two exotic species, re-sampling of nodules in the field and competition experiments between the three species were conducted. The results showed that the predominant microsymbiont associated with chickpea grown in Xinjiang was still M. muleiense , but the predominant genotypes of M. muleiense had changed significantly during the four years since a previous survey. The data also showed that M. mediterraneum and M. ciceri were more competitive than the residential strain of M. muleiense CCBAU 83963 T in sterilized vermiculite or Soils from Xinjiang. However, in non-sterilized Soils, M. muleiense was the predominant nodule occupier. These results indicated that natural or adapting evolution of M. muleiense was occurring in fields subjected to changing environmental factors. In addition, the biogeography and symbiotic associations of rhizobia with their host legumes were also influenced by biological factors in the soil, such as indigenous rhizobia and other organisms.
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distinctive mesorhizobium populations associated with cicer arietinum l in Alkaline Soils of xinjiang china
Plant and Soil, 2012Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Kai Lou, Xiang Jin, Pei Hong Mao, Chang Fu Tian, Xin Hua Sui, Wen Xin ChenAbstract:Rhizobia associated with chickpea in the main chickpea production zone of Xinjiang, China have never been investigated. Here, we present the first systematic investigation of these rhizobia’s genetic diversity and symbiotic interactions with their host plant. Ninety-five isolates obtained from chickpea nodules in eight Alkaline-saline (pH 8.24–8.45) sites in Xinjiang were characterized by nodulation test, symbiotic gene analysis, PCR-based restriction fragment length polymorphism (RFLP) of the 16S rRNA gene and 16S–23S rRNA intergenic spacer (IGS), BOX-PCR, phylogenies of 16S rRNA and housekeeping genes (atpD, recA and glnII), multilocus sequence analysis (MLSA) and DNA–DNA hybridization. All 95 isolates were identified within the genus of Mesorhizobium. Similarities less than 96.5% in MLSA and DNA–DNA hybridization values ( 98%) of symbiotic genes (nodC and nifH) with those of the well studied chickpea microsymbioints Mesorhizobium ciceri and Mesorhizobium mediterraneum were found. Chickpea rhizobia in Alkaline-saline Soils of Xinjiang, China, form a population distinct from the defined Mesorhizobium species. All these chickpea rhizobia in Xinjiang harbored symbiotic genes highly similar to the type strains of two well-studied chickpea rhizobia, M. ciceri and M. mediterraneum, evidencing the possible lateral transfer of symbiotic genes among these different rhizobial species. On the other hand, chickpea may strongly select rhizobia with a unique symbiotic gene background.
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distinctive mesorhizobium populations associated with cicer arietinum l in Alkaline Soils of xinjiang china
Plant and Soil, 2012Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Chang Fu Tian, Wen Xin ChenAbstract:Background and aims Rhizobia associated with chickpea in the main chickpea production zone of Xinjiang, China have never been investigated. Here, we present the first systematic investigation of these rhizobia’s genetic diversity and symbiotic interactions with their host plant.
Wen Xin Chen - One of the best experts on this subject based on the ideXlab platform.
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genotypic alteration and competitive nodulation of mesorhizobium muleiense against exotic chickpea rhizobia in Alkaline Soils
Systematic and Applied Microbiology, 2014Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Tao Yu, Wen Xin ChenAbstract:Abstract Mesorhizobium muleiense , Mesorhizobium mediterraneum and Mesorhizobium ciceri are chickpea ( Cicer arietinum L.) rhizobia that share a high similarity of the symbiotic genes nodC and nifH , but they have different geographic distributions. M. muleiense has been isolated and found only in Alkaline Soils of Xinjiang, China, whereas the other two strains have been found in the Mediterranean and India. To investigate the species stability of M. muleiense during natural evolution and its capability of competitive nodulation against the other two exotic species, re-sampling of nodules in the field and competition experiments between the three species were conducted. The results showed that the predominant microsymbiont associated with chickpea grown in Xinjiang was still M. muleiense , but the predominant genotypes of M. muleiense had changed significantly during the four years since a previous survey. The data also showed that M. mediterraneum and M. ciceri were more competitive than the residential strain of M. muleiense CCBAU 83963 T in sterilized vermiculite or Soils from Xinjiang. However, in non-sterilized Soils, M. muleiense was the predominant nodule occupier. These results indicated that natural or adapting evolution of M. muleiense was occurring in fields subjected to changing environmental factors. In addition, the biogeography and symbiotic associations of rhizobia with their host legumes were also influenced by biological factors in the soil, such as indigenous rhizobia and other organisms.
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distinctive mesorhizobium populations associated with cicer arietinum l in Alkaline Soils of xinjiang china
Plant and Soil, 2012Co-Authors: Jun Jie Zhang, En Tao Wang, Wen-feng Chen, Kai Lou, Xiang Jin, Pei Hong Mao, Chang Fu Tian, Xin Hua Sui, Wen Xin ChenAbstract:Rhizobia associated with chickpea in the main chickpea production zone of Xinjiang, China have never been investigated. Here, we present the first systematic investigation of these rhizobia’s genetic diversity and symbiotic interactions with their host plant. Ninety-five isolates obtained from chickpea nodules in eight Alkaline-saline (pH 8.24–8.45) sites in Xinjiang were characterized by nodulation test, symbiotic gene analysis, PCR-based restriction fragment length polymorphism (RFLP) of the 16S rRNA gene and 16S–23S rRNA intergenic spacer (IGS), BOX-PCR, phylogenies of 16S rRNA and housekeeping genes (atpD, recA and glnII), multilocus sequence analysis (MLSA) and DNA–DNA hybridization. All 95 isolates were identified within the genus of Mesorhizobium. Similarities less than 96.5% in MLSA and DNA–DNA hybridization values ( 98%) of symbiotic genes (nodC and nifH) with those of the well studied chickpea microsymbioints Mesorhizobium ciceri and Mesorhizobium mediterraneum were found. Chickpea rhizobia in Alkaline-saline Soils of Xinjiang, China, form a population distinct from the defined Mesorhizobium species. All these chickpea rhizobia in Xinjiang harbored symbiotic genes highly similar to the type strains of two well-studied chickpea rhizobia, M. ciceri and M. mediterraneum, evidencing the possible lateral transfer of symbiotic genes among these different rhizobial species. On the other hand, chickpea may strongly select rhizobia with a unique symbiotic gene background.
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distinctive mesorhizobium populations associated with cicer arietinum l in Alkaline Soils of xinjiang china
Plant and Soil, 2012Co-Authors: Jun Jie Zhang, Wen-feng Chen, En Tao Wang, Chang Fu Tian, Wen Xin ChenAbstract:Background and aims Rhizobia associated with chickpea in the main chickpea production zone of Xinjiang, China have never been investigated. Here, we present the first systematic investigation of these rhizobia’s genetic diversity and symbiotic interactions with their host plant.
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unique community structure and biogeography of soybean rhizobia in the saline Alkaline Soils of xinjiang china
Plant and Soil, 2009Co-Authors: Lili Han, En Tao Wang, Wen-feng Chen, Xin Hua Sui, Tian Xu Han, Jie Liu, Wen Xin ChenAbstract:To investigate the community composition and biogeography of soybean rhizobia in Xinjiang, a total of 151 strains were investigated with RFLP and phylogenetic analyses of 16S rRNA gene, 16S–23S intergenic spacer, three housekeeping genes (atpD, glnII and recA), and two symbiotic genes (nifH and nodC), as well as cross-nodulation. Two rhizobial species, Bradyrhizobium liaoningense and Sinorhizobium fredii, were found as dominant groups in communities of soybean rhizobia in Xinjiang, whereas three Rhizobium genomic species, B. yuanmingense and B. japonicum, were minor groups. These genomic species showed clear correlations with eco-regions, and their symbiotic genes were identical or very similar to those of the reference strains for the corresponding species. Conclusively, the dominant soybean rhizobia S. fredii and B. liaoningense in Xinjiang might be introduced from other Chinese regions, but they have been selected as the rhizobia adapted to the saline-Alkaline Soils. The high pH, salinity, and phosphate concentration in soil might be the environmental factors determining the biogeography of these bacteria. It is worth mentioning that a novel Rhizobium species that may have acquired the symbiotic genes from a Bradyrhizobium lineage was identified.