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Peidu Chen - One of the best experts on this subject based on the ideXlab platform.
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development of intron targeting it markers specific for chromosome arm 4vs of haynaldia Villosa by chromosome sorting and next generation sequencing
BMC Genomics, 2017Co-Authors: Haiyan Wang, Peidu Chen, Shouzhong Zhang, Jin Xiao, Aizhong Cao, Keli Dai, Chunxia Yuan, Renhui Zhao, Jaroslav Doležel, Xiue WangAbstract:Haynaldia Villosa (L.) Schur (syn. Dasypyrum villosum L. Candargy, 2n = 14, genome VV) is the tertiary gene pool of wheat, and thus a potential resource of genes for wheat improvement. Among other, wheat yellow mosaic (WYM) resistance gene Wss1 and a take-all resistance gene were identified on the short arm of chromosome 4 V (4VS) of H. Villosa. We had obtained introgressions on 4VS chromosome arm, with the objective of utilizing the target genes. However, monitoring these introgressions has been a daunting task because of inadequate knowledge as to H.Villosa genome, as reflected by the lack of specific markers. This study aims to develop 4VS-specific markers by combination of chromosome sorting and next-generation sequencing. The short arm of chromosome 4VS of H.Villosa was flow-sorted using a FACSVantage SE flow cytometer and sorter, and then sequenced by Illumina sequencing. The sequence of H. Villosa 4VS was assembled by the software Hecate, and then was compared with the sequence assemblies of wheat chromosome arms 4AL, 4BS and 4DS and Ae. tauschii 4DS, with the objectives of identifying exon-exon junctions and localizing introns on chromosome 4VS of H. Villosa. The intron length polymorphisms suitable for designing H. Villosa primers were evaluated with criteria. Consequently, we designed a total of 359 intron targeting (IT) markers, among which 232 (64.62%) markers were specific for tracing the 4VS chromatin in the wheat background. The combination of chromosome sorting and next-generation sequencing to develop specific IT markers for 4VS of H. Villosa has high success rate and specificity, thus being applicable for the development of chromosome-specific markers for alien chromatin in wheat breeding.
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radiation induced translocations with reduced haynaldia Villosa chromatin at the pm21 locus for powdery mildew resistance in wheat
Molecular Breeding, 2013Co-Authors: Peidu Chen, Bo Zhou, Aizhong Cao, Chunfang You, Shengwei Chen, Xiue WangAbstract:Haynaldia Villosa Schur. (syn. Dasypyrum villosum Candargy, 2n = 2x = 14, genome VV), a species related to wheat, is highly resistant to powdery mildew. The powdery mildew resistance gene Pm21 from H. Villosa was introduced into common wheat by means of a translocation line T6VS·6AL, where the 6VS chromosome arm of H. Villosa was joined at the centromere with wheat chromosome arm 6AL. To develop small alien translocations, especially interstitial translocations of small alien chromosome segments, we irradiated mature female gametes of a T6VS·6AL translocation line with gamma rays. More than 20 new translocations and deletions of 6V chromatin were obtained and subsequently used to map Pm21. Pm21 was located in a small region (FL 0.45–0.58) by genomic in situ hybridization, molecular marker analysis, and powdery mildew response. Two homozygous translocation lines with small H. Villosa chromosome fragments carrying Pm21 were identified by fluorescence in situ hybridization and molecular marker analysis: an interstitial translocation in which a small fragment of 6VS is inserted into chromosome 4B and a terminal translocation with a small fragment of 6VS inserted into 1A. These small alien translocations are being transferred into an adapted elite wheat background by backcrossing to allow their easy use in breeding programs.
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distribution of highly repeated dna sequences in haynaldia Villosa and its application in the identification of alien chromatin
Chinese Science Bulletin, 2013Co-Authors: Wei Zhang, Ruiqi Zhang, Yigao Feng, Tongde Bie, Peidu ChenAbstract:Haynaldia Villosa (L.) is a wild relative species of common wheat that possesses many beneficial genes that can be used for wheat improvement. The accurate detection of H. Villosa chromosomes in the genetic background of wheat is critical for transferring its beneficial genes to common wheat by chromosome engineering. The aim of the present study was to investigate the distribution patterns of two repeated DNA sequences, pSc119.2 and pAs1, as well as two rDNA multigene family sequences, 45S rDNA and 5S rDNA, in the individual chromosomes of H. Villosa for the future precise identification of alien chromatin in germplasm development and breeding programs. A set of common wheat-H. Villosa disomic addition 1V-7V lines was used to determine these specific signals on individual chromosomes of H. Villosa. The results showed that two rDNA probes, pTa71 (45S rDNA) and pTa794 (5S rDNA), were located on 1VS and 5VS, respectively, and the signal could be discriminated exclusively in the common wheat background as effective markers of 1VS and 5VS. Furthermore, all seven chromosomes of H. Villosa could be distinguished clearly by fluorescence in situ hybridization using pSc119.2 and pAs1 as probes in combination. The utilization of these cytogenetic markers of repetitive sequences, combined with other molecular markers sometimes, will make it possible for a precise identification of alien chromosomes with high efficiency.
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establishment of an effective virus induced gene silencing system with bsmv in haynaldia Villosa
Molecular Biology Reports, 2010Co-Authors: Xiaoyun Wang, Xiue Wang, Aizhong Cao, Daowen Wang, Peidu ChenAbstract:In recent years, virus-induced gene-silencing (VIGS) has shown to be a powerful reverse genetics tool for gene function study. In this paper, an effective and persistent virus-induced gene silencing (VIGS) system was established with barley stripe mosaic virus (BSMV) for Haynaldia Villosa. Examination of GFP gene expression showed that the BSMV vector moved systemically from leaf to leaf in inoculated H. Villosa plants. This vector, expressing a cDNA fragment of phytoene desaturase (PDS), suppressed the transcript level of endogenous Hv-PDS gene as early as 6 days after inoculation, and caused photobleaching symptoms mainly on the newly developed upper leaves. Moreover, PDS gene silencing phenotype persisted through the whole growing period in H. Villosa. With this established VIGS system, function analysis of a powdery mildew resistance related gene Hv-LRR was successfully performed. This is the first report that BSMV can be used for VIGS in a wild relative species of wheat. The established VIGS system will be a powerful reverse genetics tool for gene function study in H. Villosa, an important genetic resource for wheat breeding.
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screening and application of est based pcr markers specific to individual chromosomes of haynaldia Villosa
Acta Agronomica Sinica, 2009Co-Authors: Aizhong Cao, Yaping Cao, Xiue Wang, Peidu ChenAbstract:Abstract In our previous studies, a series of wheat– Haynaldia Villosa alien chromosome lines including translocation lines were developed through pollen-irradiation induction. To identify H. Villosa chromosomes or chromosome segments in these lines, 240 sequence tagged site (STS) primer pairs were designed based on expressed sequence tags (ESTs) of rice ( Oryza sativa L.) and wheat, and 34 of them amplified specific polymorphic bands between T. aestivum cv. Chinese Spring and H. Villosa . These 34 STS primer pairs were further used for screening markers specific to individual chromosomes of H. Villosa using a complete set of disomic addition lines. Marker CINAU32 −300 could be used for tracing chromosome 1V, markers CINAU33 −280 , CINAU34 −510 , CINAU35 −1100 , CINAU36 −380 , and CINAU37 −400 for 2V, marker CINAU38 −250 for 3V, markers CINAU39 −950 , and CINAU40 −800 for 4V, markers CINAU41 −745 and CINAU42 −1050 for 5V, and markers CINAU44 −765 and CINAU45 −495 for 7V. Chromosomes and chromosome segments from H. Villosa in the backcrossed generations derived from pollen irradiation were characterized using these EST-STS markers combined with two 6V specific markers that developed in earlier studies. A complete set of 1V to 7V addition lines and 18 translocation lines involving different H. Villosa chromosomes were identified. Therefore, these chromosome-specific EST-STS markers are useful for detecting H. Villosa chromosomes and chromosome segments in common wheat background.
Miguel Ángel Cantamutto - One of the best experts on this subject based on the ideXlab platform.
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vicia Villosa ssp Villosa roth field emergence model in a semiarid agroecosystem
Grass and Forage Science, 2018Co-Authors: Juan P. Renzi, Guillermo R. Chantre, Miguel Ángel CantamuttoAbstract:Hairy vetch (Vicia Villosa Roth) is a winter annual legume cultivated for pasture and hay with the capability for natural reseeding. Vicia Villosa increases N concentrations in the soil, thus contributing to the sustainability of semiarid regions. However, under rotations of 1–2 years of pasture followed by 1 year of crop (1:1–2:1), hairy vetch could become a problematic volunteer weed in the winter cereal crop phase. This study aimed to develop a mechanistic model for hairy vetch seedling emergence in order to (i) estimate the natural reseeding of hairy vetch in the pasture phase of the field rotation, or (ii) develop control strategies considering hairy vetch as a volunteer weed in the winter cereal phase. The proposed model simulates the pattern of field emergence of hairy vetch after natural seed dispersal by integrating four submodels: (i) physical (PY) dormancy release dynamics, (ii) physiological dormancy (PD) release and germination thermal requirements, (iii) hydro-time requirements for germination, and (iv) pre-emergence growth respectively. The developed field emergence model was validated with independent field emergence data during 2013, 2014 and 2015. The model adequately predicted the timing and magnitude of field emergence flushes (RMSE < 10.1) despite the environmental variability among years. The additive effect of each submodel clearly improved the explanatory capacity of the field emergence patterns. The alleviation of PD synchronizes the timing for hairy vetch germination, while the PY determines the seedbank persistence. These outcomes suggest the potential applicability of the proposed modelling approach within management decision support systems.
Guillermo R. Chantre - One of the best experts on this subject based on the ideXlab platform.
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vicia Villosa ssp Villosa roth field emergence model in a semiarid agroecosystem
Grass and Forage Science, 2018Co-Authors: Juan P. Renzi, Guillermo R. Chantre, Miguel Ángel CantamuttoAbstract:Hairy vetch (Vicia Villosa Roth) is a winter annual legume cultivated for pasture and hay with the capability for natural reseeding. Vicia Villosa increases N concentrations in the soil, thus contributing to the sustainability of semiarid regions. However, under rotations of 1–2 years of pasture followed by 1 year of crop (1:1–2:1), hairy vetch could become a problematic volunteer weed in the winter cereal crop phase. This study aimed to develop a mechanistic model for hairy vetch seedling emergence in order to (i) estimate the natural reseeding of hairy vetch in the pasture phase of the field rotation, or (ii) develop control strategies considering hairy vetch as a volunteer weed in the winter cereal phase. The proposed model simulates the pattern of field emergence of hairy vetch after natural seed dispersal by integrating four submodels: (i) physical (PY) dormancy release dynamics, (ii) physiological dormancy (PD) release and germination thermal requirements, (iii) hydro-time requirements for germination, and (iv) pre-emergence growth respectively. The developed field emergence model was validated with independent field emergence data during 2013, 2014 and 2015. The model adequately predicted the timing and magnitude of field emergence flushes (RMSE < 10.1) despite the environmental variability among years. The additive effect of each submodel clearly improved the explanatory capacity of the field emergence patterns. The alleviation of PD synchronizes the timing for hairy vetch germination, while the PY determines the seedbank persistence. These outcomes suggest the potential applicability of the proposed modelling approach within management decision support systems.
Aizhong Cao - One of the best experts on this subject based on the ideXlab platform.
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development of intron targeting it markers specific for chromosome arm 4vs of haynaldia Villosa by chromosome sorting and next generation sequencing
BMC Genomics, 2017Co-Authors: Haiyan Wang, Peidu Chen, Shouzhong Zhang, Jin Xiao, Aizhong Cao, Keli Dai, Chunxia Yuan, Renhui Zhao, Jaroslav Doležel, Xiue WangAbstract:Haynaldia Villosa (L.) Schur (syn. Dasypyrum villosum L. Candargy, 2n = 14, genome VV) is the tertiary gene pool of wheat, and thus a potential resource of genes for wheat improvement. Among other, wheat yellow mosaic (WYM) resistance gene Wss1 and a take-all resistance gene were identified on the short arm of chromosome 4 V (4VS) of H. Villosa. We had obtained introgressions on 4VS chromosome arm, with the objective of utilizing the target genes. However, monitoring these introgressions has been a daunting task because of inadequate knowledge as to H.Villosa genome, as reflected by the lack of specific markers. This study aims to develop 4VS-specific markers by combination of chromosome sorting and next-generation sequencing. The short arm of chromosome 4VS of H.Villosa was flow-sorted using a FACSVantage SE flow cytometer and sorter, and then sequenced by Illumina sequencing. The sequence of H. Villosa 4VS was assembled by the software Hecate, and then was compared with the sequence assemblies of wheat chromosome arms 4AL, 4BS and 4DS and Ae. tauschii 4DS, with the objectives of identifying exon-exon junctions and localizing introns on chromosome 4VS of H. Villosa. The intron length polymorphisms suitable for designing H. Villosa primers were evaluated with criteria. Consequently, we designed a total of 359 intron targeting (IT) markers, among which 232 (64.62%) markers were specific for tracing the 4VS chromatin in the wheat background. The combination of chromosome sorting and next-generation sequencing to develop specific IT markers for 4VS of H. Villosa has high success rate and specificity, thus being applicable for the development of chromosome-specific markers for alien chromatin in wheat breeding.
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a disulphide isomerase gene pdi v from haynaldia Villosa contributes to powdery mildew resistance in common wheat
Scientific Reports, 2016Co-Authors: Muhammad Faheem, Zongkuan Wang, Haiyan Wang, Jin Xiao, Aizhong Cao, Muhammad Arshad, Cheng Jiangyue, Zhao Jia, Liping Xing, Ruiqi ZhangAbstract:In this study, we report the contribution of a PDI-like gene from wheat wild relative Haynaldia Villosa in combating powdery mildew. PDI-V protein contains two conserved thioredoxin (TRX) active domains (a and a') and an inactive domain (b). PDI-V interacted with E3 ligase CMPG1-V protein, which is a positive regulator of powdery mildew response. PDI-V was mono-ubiquitinated by CMPG1-V without degradation being detected. PDI-V was located on H. Villosa chromosome 5V and encoded for a protein located in the endoplasmic reticulum. Bgt infection in leaves of H. Villosa induced PDI-V expression. Virus induced gene silencing of PDIs in a T. durum-H. Villosa amphiploid compromised the resistance. Single cell transient over-expression of PDI-V or a truncated version containing the active TXR domain a decreased the haustorial index in moderately susceptible wheat cultivar Yangmai 158. Stable transgenic lines over-expressing PDI-V in Yangmai 158 displayed improved powdery mildew resistance at both the seedling and adult stages. By contrast over-expression of point-mutated PDI-V(C57A) did not increase the level of resistance in Yangmai 158. The above results indicate a pivotal role of PDI-V in powdery mildew resistance and showed that conserved TRX domain a is critical for its function.
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e3 ubiquitin ligase gene cmpg1 v from haynaldia Villosa l contributes to powdery mildew resistance in common wheat triticum aestivum l
Plant Journal, 2015Co-Authors: Yanfei Zhu, Zongkuan Wang, Haiyan Wang, Aizhong Cao, Liping Xing, Fei Fei, Wei Wang, Yuan Liu, Shuang Han, Wei ChenAbstract:Powdery mildew is one of the most devastating wheat fungal diseases. A diploid wheat relative, Haynaldia Villosa L., is highly resistant to powdery mildew, and its genetic resource of resistances, such as the Pm21 locus, is now widely used in wheat breeding. Here we report the cloning of a resistance gene from H. Villosa, designated CMPG1-V, that encodes a U-box E3 ubiquitin ligase. Expression of the CMPG1-V gene was induced in the leaf and stem of H. Villosa upon inoculation with Blumeria graminis f. sp. tritici (Bgt) fungus, and the presence of Pm21 is essential for its rapid induction of expression. CMPG1-V has conserved key residues for E3 ligase, and possesses E3 ligase activity in vitro and in vivo. CMPG1-V is localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans-Golgi network/early endosome vesicles. Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid-responsive genes, H2 O2 accumulation, and cell-wall protein cross-linking at the Bgt infection sites, and the expression of CMPG1-V in H. Villosa was increased when treated with salicylic acid, abscisic acid and H2 O2 . These results indicate the involvement of E3 ligase in defense responses to Bgt fungus in wheat, particularly in broad-spectrum disease resistance, and suggest association of reactive oxidative species and the phytohormone pathway with CMPG1-V-mediated powdery mildew resistance.
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radiation induced translocations with reduced haynaldia Villosa chromatin at the pm21 locus for powdery mildew resistance in wheat
Molecular Breeding, 2013Co-Authors: Peidu Chen, Bo Zhou, Aizhong Cao, Chunfang You, Shengwei Chen, Xiue WangAbstract:Haynaldia Villosa Schur. (syn. Dasypyrum villosum Candargy, 2n = 2x = 14, genome VV), a species related to wheat, is highly resistant to powdery mildew. The powdery mildew resistance gene Pm21 from H. Villosa was introduced into common wheat by means of a translocation line T6VS·6AL, where the 6VS chromosome arm of H. Villosa was joined at the centromere with wheat chromosome arm 6AL. To develop small alien translocations, especially interstitial translocations of small alien chromosome segments, we irradiated mature female gametes of a T6VS·6AL translocation line with gamma rays. More than 20 new translocations and deletions of 6V chromatin were obtained and subsequently used to map Pm21. Pm21 was located in a small region (FL 0.45–0.58) by genomic in situ hybridization, molecular marker analysis, and powdery mildew response. Two homozygous translocation lines with small H. Villosa chromosome fragments carrying Pm21 were identified by fluorescence in situ hybridization and molecular marker analysis: an interstitial translocation in which a small fragment of 6VS is inserted into chromosome 4B and a terminal translocation with a small fragment of 6VS inserted into 1A. These small alien translocations are being transferred into an adapted elite wheat background by backcrossing to allow their easy use in breeding programs.
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establishment of an effective virus induced gene silencing system with bsmv in haynaldia Villosa
Molecular Biology Reports, 2010Co-Authors: Xiaoyun Wang, Xiue Wang, Aizhong Cao, Daowen Wang, Peidu ChenAbstract:In recent years, virus-induced gene-silencing (VIGS) has shown to be a powerful reverse genetics tool for gene function study. In this paper, an effective and persistent virus-induced gene silencing (VIGS) system was established with barley stripe mosaic virus (BSMV) for Haynaldia Villosa. Examination of GFP gene expression showed that the BSMV vector moved systemically from leaf to leaf in inoculated H. Villosa plants. This vector, expressing a cDNA fragment of phytoene desaturase (PDS), suppressed the transcript level of endogenous Hv-PDS gene as early as 6 days after inoculation, and caused photobleaching symptoms mainly on the newly developed upper leaves. Moreover, PDS gene silencing phenotype persisted through the whole growing period in H. Villosa. With this established VIGS system, function analysis of a powdery mildew resistance related gene Hv-LRR was successfully performed. This is the first report that BSMV can be used for VIGS in a wild relative species of wheat. The established VIGS system will be a powerful reverse genetics tool for gene function study in H. Villosa, an important genetic resource for wheat breeding.
Xiue Wang - One of the best experts on this subject based on the ideXlab platform.
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development of intron targeting it markers specific for chromosome arm 4vs of haynaldia Villosa by chromosome sorting and next generation sequencing
BMC Genomics, 2017Co-Authors: Haiyan Wang, Peidu Chen, Shouzhong Zhang, Jin Xiao, Aizhong Cao, Keli Dai, Chunxia Yuan, Renhui Zhao, Jaroslav Doležel, Xiue WangAbstract:Haynaldia Villosa (L.) Schur (syn. Dasypyrum villosum L. Candargy, 2n = 14, genome VV) is the tertiary gene pool of wheat, and thus a potential resource of genes for wheat improvement. Among other, wheat yellow mosaic (WYM) resistance gene Wss1 and a take-all resistance gene were identified on the short arm of chromosome 4 V (4VS) of H. Villosa. We had obtained introgressions on 4VS chromosome arm, with the objective of utilizing the target genes. However, monitoring these introgressions has been a daunting task because of inadequate knowledge as to H.Villosa genome, as reflected by the lack of specific markers. This study aims to develop 4VS-specific markers by combination of chromosome sorting and next-generation sequencing. The short arm of chromosome 4VS of H.Villosa was flow-sorted using a FACSVantage SE flow cytometer and sorter, and then sequenced by Illumina sequencing. The sequence of H. Villosa 4VS was assembled by the software Hecate, and then was compared with the sequence assemblies of wheat chromosome arms 4AL, 4BS and 4DS and Ae. tauschii 4DS, with the objectives of identifying exon-exon junctions and localizing introns on chromosome 4VS of H. Villosa. The intron length polymorphisms suitable for designing H. Villosa primers were evaluated with criteria. Consequently, we designed a total of 359 intron targeting (IT) markers, among which 232 (64.62%) markers were specific for tracing the 4VS chromatin in the wheat background. The combination of chromosome sorting and next-generation sequencing to develop specific IT markers for 4VS of H. Villosa has high success rate and specificity, thus being applicable for the development of chromosome-specific markers for alien chromatin in wheat breeding.
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radiation induced translocations with reduced haynaldia Villosa chromatin at the pm21 locus for powdery mildew resistance in wheat
Molecular Breeding, 2013Co-Authors: Peidu Chen, Bo Zhou, Aizhong Cao, Chunfang You, Shengwei Chen, Xiue WangAbstract:Haynaldia Villosa Schur. (syn. Dasypyrum villosum Candargy, 2n = 2x = 14, genome VV), a species related to wheat, is highly resistant to powdery mildew. The powdery mildew resistance gene Pm21 from H. Villosa was introduced into common wheat by means of a translocation line T6VS·6AL, where the 6VS chromosome arm of H. Villosa was joined at the centromere with wheat chromosome arm 6AL. To develop small alien translocations, especially interstitial translocations of small alien chromosome segments, we irradiated mature female gametes of a T6VS·6AL translocation line with gamma rays. More than 20 new translocations and deletions of 6V chromatin were obtained and subsequently used to map Pm21. Pm21 was located in a small region (FL 0.45–0.58) by genomic in situ hybridization, molecular marker analysis, and powdery mildew response. Two homozygous translocation lines with small H. Villosa chromosome fragments carrying Pm21 were identified by fluorescence in situ hybridization and molecular marker analysis: an interstitial translocation in which a small fragment of 6VS is inserted into chromosome 4B and a terminal translocation with a small fragment of 6VS inserted into 1A. These small alien translocations are being transferred into an adapted elite wheat background by backcrossing to allow their easy use in breeding programs.
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establishment of an effective virus induced gene silencing system with bsmv in haynaldia Villosa
Molecular Biology Reports, 2010Co-Authors: Xiaoyun Wang, Xiue Wang, Aizhong Cao, Daowen Wang, Peidu ChenAbstract:In recent years, virus-induced gene-silencing (VIGS) has shown to be a powerful reverse genetics tool for gene function study. In this paper, an effective and persistent virus-induced gene silencing (VIGS) system was established with barley stripe mosaic virus (BSMV) for Haynaldia Villosa. Examination of GFP gene expression showed that the BSMV vector moved systemically from leaf to leaf in inoculated H. Villosa plants. This vector, expressing a cDNA fragment of phytoene desaturase (PDS), suppressed the transcript level of endogenous Hv-PDS gene as early as 6 days after inoculation, and caused photobleaching symptoms mainly on the newly developed upper leaves. Moreover, PDS gene silencing phenotype persisted through the whole growing period in H. Villosa. With this established VIGS system, function analysis of a powdery mildew resistance related gene Hv-LRR was successfully performed. This is the first report that BSMV can be used for VIGS in a wild relative species of wheat. The established VIGS system will be a powerful reverse genetics tool for gene function study in H. Villosa, an important genetic resource for wheat breeding.
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screening and application of est based pcr markers specific to individual chromosomes of haynaldia Villosa
Acta Agronomica Sinica, 2009Co-Authors: Aizhong Cao, Yaping Cao, Xiue Wang, Peidu ChenAbstract:Abstract In our previous studies, a series of wheat– Haynaldia Villosa alien chromosome lines including translocation lines were developed through pollen-irradiation induction. To identify H. Villosa chromosomes or chromosome segments in these lines, 240 sequence tagged site (STS) primer pairs were designed based on expressed sequence tags (ESTs) of rice ( Oryza sativa L.) and wheat, and 34 of them amplified specific polymorphic bands between T. aestivum cv. Chinese Spring and H. Villosa . These 34 STS primer pairs were further used for screening markers specific to individual chromosomes of H. Villosa using a complete set of disomic addition lines. Marker CINAU32 −300 could be used for tracing chromosome 1V, markers CINAU33 −280 , CINAU34 −510 , CINAU35 −1100 , CINAU36 −380 , and CINAU37 −400 for 2V, marker CINAU38 −250 for 3V, markers CINAU39 −950 , and CINAU40 −800 for 4V, markers CINAU41 −745 and CINAU42 −1050 for 5V, and markers CINAU44 −765 and CINAU45 −495 for 7V. Chromosomes and chromosome segments from H. Villosa in the backcrossed generations derived from pollen irradiation were characterized using these EST-STS markers combined with two 6V specific markers that developed in earlier studies. A complete set of 1V to 7V addition lines and 18 translocation lines involving different H. Villosa chromosomes were identified. Therefore, these chromosome-specific EST-STS markers are useful for detecting H. Villosa chromosomes and chromosome segments in common wheat background.
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a sequence specific pcr marker linked with pm21 distinguishes chromosomes 6as 6bs 6ds of triticum aestivum and 6vs of haynaldia Villosa
Plant Breeding, 2006Co-Authors: Aizhong Cao, Xiue Wang, Yaping Chen, X W Zou, Peidu ChenAbstract:To develop markers linked with Pm21 located on chromosome 6VS of Haynaldia Villosa, a pair of primers (NAU/xibaol5F and NAU/ xibaol5R) were designed according to the sequence of a serine/ threonine kinase gene (Contigl7515), whose expression was induced by Blumeria graminis and selected from the gene expression experiment using the Barley GeneChip. Using genomic DNA of various genetic stocks including the wheat variety 'Yangmai#5', H. Villosa, Triticum durum-H. Villosa amphiploid, seven T. aestivum-H. Villosa addition lines involving chromosomes 1V-7V, the translocation line T6VS-6AL, and 21 nullisomic-tetrasomic and eight deletion lines of T. aestivum 'Chinese Spring' as templates, four amplicons specific for 6VS, 6AS, 6BS and 6DS, respectively, were produced. F 2 individuals derived from the cross of 'Yangmai#5' x T6VS·6AL were analysed, and data indicate that NAU/xibao15 902 could be used as a co-dominant marker for selecting Pm21 located on 6VS.
