Allethrin

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Erik Perdana Putra - One of the best experts on this subject based on the ideXlab platform.

  • pengaruh air kelapa muda cocos nucifera terhadap kualitas spermatozoa mencit mus musculus galur balb c yang terpapar Allethrin sebagai sumber belajar pada mata kuliah fisiologi hewan erik perdana putra
    SKRIPSI Mahasiswa UM, 2019
    Co-Authors: Erik Perdana Putra
    Abstract:

    ABSTRAK Putra, Erik. P. 2012. Pengaruh Air Kelapa Muda (Cocos nucifera) Terhadap Kualitas Spermatozoa Mencit (Mus musculus) Galur Balb C Yang Terpapar Allethrin Sebagai Sumber Belajar Pada Mata Kuliah Fisiologi Hewan. Tesis, Program Studi Pendidikan Biologi, Program Pascasarjana, Universitas Negeri Malang. Pembimbing: (I) Dr. Umie Lestari, M.Si dan (II) Dr. Abdul Ghofur, M.Si Kata Kunci: Air kelapa muda, spermatozoa, Allethrin Tanaman dan hewan menjadi objek yang sering dimanfaatkan oleh masyarakat Indonesia sebagai obat-obatan tradisional untuk penyembuhan berbagai macam penyakit, salah satu contoh tanaman tersebut adalah kelapa. Khususnya buah kelapa muda, airnya memiliki banyak khasiat yang dapat digunakan sebagai obat. Air kelapa muda mengandung vitamin C 2,2-3,4 mg/100 ml, sejumlah mineral nitrogen, fosfor, kalium, magnesium, klorin, sulfur, dan besi. Selain itu, juga terkandung micronutrien lain seperti thiamin, riboflavin, asam folat dan selenium. Vitamin C dan mineral-mineral tersebut berperan sebagai antioksidan untuk menangkal dan menetralisir radikal bebas seperti senyawa allethtrin dari obat nyamuk bakar maupun elektrik. Allethrin adalah senyawa aktif berupa produk sintetik dari bahan kimia yang digunakan dalam insektisida komersial yang berasal dari insektisida alami pyrethrum yang disintesis dari bunga krisan (Chrysanthenim cinerariafolium). Penelitian ini bertujuan: (1) untuk menganalisis pengaruh pemberian air kelapa muda (Cocos nucifera) terhadap kualitas spermatozoa mencit (Mus musculus) galur balb c yang terpapar Allethrin, (2) untuk mengevaluasi dan menghasilkan modul berbasis riset yang berisikan hasil penelitian pengaruh air kelapa muda (Cocos nucifera) terhadap kualitas spermatozoa mencit (Mus musculus) galur balb c yang terpapar Allethrin yang dapat dipergunakan sebagai sumber belajar pada mata kuliah Fisiologi Hewan. Penelitian ini menggunakan pendekatan kuantitatif eksperimen dengan model rancangan acak lengkap (RAL). Terdapat 5 kelompok perlakuan dan masing-masing 5 ulangan, kelompok eksperimen diberikan konsentrasi air kelapa muda yang berbeda yaitu 25%, 50%, 75% dan 100% dan kelompok kontrol dengan aquades selama 36 hari. Setiap kelompok perlakuan sebelumnya juga diberikan paparan Allethrin dari obat nyamuk elektrik selama 8 jam per hari. Pengambilan data dilakukan dengan cara mencacah kauda epididimis di dalam NaCl 0,9% sampai terbentuk suspensi, kemudian diamati kualitas spermatozoa yang meliputi konsentrasi, morfologi, viabilitas dan motilitas. Data yang diperoleh dianalisis dengan menggunakan analisis varian satu jalur (One Way ANOVA). Hasil penelitian menunjukkan adanya pengaruh air kelapa muda terhadap kualitas spermatozoa mencit yang sebelumnya diberikan paparan Allethrin. Air kelapa muda pada konsentrasi 100% menunjukkan pengaruh yang signifikan dalam meningkatkan konsentrasi spermatozoa. Pada indikator morfologi, viabilitas dan motilitas, air kelapa muda pada konsentrasi 50% mulai berpengaruh secara signifikan dalam meningkatkan kualitas spermatozoa mencit. Berdasarkan hasil penelitian ini dan kajian pustaka yang relevan, disusun sebuah bahan ajar berupa modul bagi mahasiswa pada mata kuliah Fisiologi Hewan pada pokok bahasan Sistem Reproduksi.

  • pengaruh air kelapa muda cocos nucifera terhadap kualitas spermatozoa mencit mus musculus galur balb c yang terpapar Allethrin sebagai sumber belajar pada mata kuliah fisiologi hewan tesis
    DISERTASI dan TESIS Program Pascasarjana UM, 2013
    Co-Authors: Erik Perdana Putra, Umie Lestari, Abdul Ghofur
    Abstract:

    Plants and animals are often the object used by the people of Indonesia as a traditional medicine to cure various diseases , one example is the coconut . Especially young coconuts , its water has many benefits that can be used as medicine . Young coconut water contains vitamin C 2.2 to 3.4 mg/100 ml , a number of mineral nitrogen , phosphorus , potassium , magnesium , chlorine , sulfur , and iron . Moreover, it also contained micronutrien such as thiamin , riboflavin , folic acid and selenium . Vitamin C and the minerals act as antioxidants to counteract and neutralize free radicals such as allethtrin of mosquito coils and electric. Allethrin is the active compound in the form of synthetic chemical products used in a commercial insecticide derived from natural insecticide pyrethrum which is synthesized from chrysanthemums ( Chrysanthenim cinerariafolium ). This study aims to : (1) to analyze the effects of coconut water ( Cocos nucifera ) on the quality of spermatozoa of mice ( Mus musculus ) strains balb c were exposed to Allethrin , (2) to evaluate and generate research -based module that contains the results of research on the effect of young coconut water ( Cocos nucifera ) on the quality of spermatozoa of mice ( Mus musculus ) strain balb c were exposed to Allethrin that can be used as a learning resource on Animal Physiology Subject . This study uses a quantitative experiment approach the design followed randomized completely design . There are 5 treatment groups and each of the 5 repetition, the experimental group was given the different concentration of coconut water is 25 %, 50% , 75% and 100 % and the control group with aquades for 36 days . Each treatment group had also given exposure to Allethrin of electrical coils for 8 hours per day. Data retrieval is done by chopping cauda epididymis in 0.9% NaCl to form a suspension , and then observed the quality of spermatozoa which includes concentration , morphology , viability and motility . Data were analyzed using one-way analysis of variance ( One Way ANOVA ). The results showed that the effect of coconut water on the quality of spermatozoa of mice previously given exposure to Allethrin . The young coconut water at a concentration of 100% indicates a significant effect in increasing the concentration of spermatozoa . At indicators morphology , viability and motility , young coconut water at a concentration of 50% began to significantly improve the quality of spermatozoa in mice . Based on the results of this study and the relevant literature , compiled a module in the form of teaching materials for the college students in Animal Physiology courses on the subject of Reproductive System . Tanaman dan hewan menjadi objek yang sering dimanfaatkan oleh masyarakat Indonesia sebagai obat-obatan tradisional untuk penyembuhan berbagai macam penyakit, salah satu contoh tanaman tersebut adalah kelapa. Khususnya buah kelapa muda, airnya memiliki banyak khasiat yang dapat digunakan sebagai obat. Air kelapa muda mengandung vitamin C 2,2-3,4 mg/100 ml, sejumlah mineral nitrogen, fosfor, kalium, magnesium, klorin, sulfur, dan besi. Selain itu, juga terkandung micronutrien lain seperti thiamin , riboflavin, asam folat dan selenium. Vitamin C dan mineral-mineral tersebut berperan sebagai antioksidan untuk menangkal dan menetralisir radikal bebas seperti senyawa allethtrin dari obat nyamuk bakar maupun elektrik. Allethrin adalah senyawa aktif berupa produk sintetik dari bahan kimia yang digunakan dalam insektisida komersial yang berasal dari insektisida alami pyrethrum yang disintesis dari bunga krisan ( Chrysanthenim cinerariafolium ). Penelitian ini bertujuan: (1) untuk menganalisis pengaruh pemberian air kelapa muda ( Cocos nucifera ) terhadap kualitas spermatozoa mencit ( Mus musculus ) galur balb c yang terpapar Allethrin , (2) untuk mengevaluasi dan menghasilkan modul berbasis riset yang berisikan hasil penelitian pengaruh air kelapa muda ( Cocos nucifera ) terhadap kualitas spermatozoa mencit ( Mus musculus ) galur balb c yang terpapar Allethrin yang dapat dipergunakan sebagai sumber belajar pada mata kuliah Fisiologi Hewan. Penelitian ini menggunakan pendekatan kuantitatif eksperimen dengan model rancangan acak lengkap (RAL). Terdapat 5 kelompok perlakuan dan masing-masing 5 ulangan, kelompok eksperimen diberikan konsentrasi air kelapa muda yang berbeda yaitu 25%, 50%, 75% dan 100% dan kelompok kontrol dengan aquades selama 36 hari. Setiap kelompok perlakuan sebelumnya juga diberikan paparan Allethrin dari obat nyamuk elektrik selama 8 jam per hari. Pengambilan data dilakukan dengan cara mencacah kauda epididimis di dalam NaCl 0,9% sampai terbentuk suspensi, kemudian diamati kualitas spermatozoa yang meliputi konsentrasi, morfologi, viabilitas dan motilitas. Data yang diperoleh dianalisis dengan menggunakan analisis varian satu jalur ( One Way ANOVA) . Hasil penelitian menunjukkan adanya pengaruh air kelapa muda terhadap kualitas spermatozoa mencit yang sebelumnya diberikan paparan Allethrin . Air kelapa muda pada konsentrasi 100% menunjukkan pengaruh yang signifikan dalam meningkatkan konsentrasi spermatozoa. Pada indikator morfologi, viabilitas dan motilitas, air kelapa muda pada konsentrasi 50% mulai berpengaruh secara signifikan dalam meningkatkan kualitas spermatozoa mencit. Berdasarkan hasil penelitian ini dan kajian pustaka yang relevan, disusun sebuah bahan ajar berupa modul bagi mahasiswa pada mata kuliah Fisiologi Hewan pada pokok bahasan Sistem Reproduksi. Keyword: young coconut water, spermatozoa, Allethrin

Suresh Yenugu - One of the best experts on this subject based on the ideXlab platform.

  • exposure to Allethrin based mosquito coil smoke during gestation and postnatal development affects reproductive function in male offspring of rat
    Inhalation Toxicology, 2017
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    AbstractThe threat of zika virus looms throughout the world and the use of Allethrin-based mosquito coils to prevent mosquito bites during and postpregnancy is on the rise. The aim of this study was to analyze the toxic effects of exposure to Allethrin-based mosquito coil smoke in rats under conditions that reflect human settings. Pregnant female rats were exposed to mosquito coil smoke and same was continued to the male pups up to 111 days postparturition (21-day weaning plus up to 90 days postweaning). Increased oxidative stress, distorted antioxidant enzyme status, downregulation of genes involved in spermatogenesis, sperm maturation and steroidogenesis was observed. Daily sperm production, total sperm count and acrosome reaction was compromised. Results of our study indicate the toxic effects of exposure to Allethrin-based mosquito coil smoke in male offspring and calls for preventing mosquito coil use during pregnancy and postnatal development. Community-based programs that will encourage general pop...

  • Allethrin toxicity causes reproductive dysfunction in male rats
    Environmental Toxicology, 2017
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Pyrethroids are widely used for domestic and agricultural purposes and their use is increasing, especially in developing countries. Uncontrolled use of these insecticides resulted in their entry into the food chain thereby causing toxicity to different organ systems. Allethrin is one of the widely used pyrethroids, but its toxicological effects are underreported when compared to other pyrethroids. Further, its effects on the male reproductive tract remain uncharacterized. In this study, its toxicity on the male reproductive tract was evaluated by administering 25–150 mg/kg body weight Allethrin to adult rats for 60 days. The mRNA expression of factors that are important in spermatogenesis (Scf, c-Kit, Hsf2, Ovol1, Brdt, Kdm3A, Ybx-2, and Grth) and steroidogenesis (StAR, 3β-HSD, 17β-HSD) was significantly downregulated. Decreased levels of testosterone, reduced sperm count and daily sperm production was also observed due to Allethrin toxicity. However, sperm quality parameters assessed by computer-assisted sperm analyzer were not affected. Spermatozoa obtained from Allethrin-treated rats failed to undergo acrosome reaction. Results of this study indicate that Allethrin affects spermatogenesis and sperm function, thus lending further support to the growing evidence of its toxicity.

  • Allethrin induces oxidative stress apoptosis and calcium release in rat testicular carcinoma cells lc540
    Toxicology in Vitro, 2014
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Abstract Over the years, pyrethroids, including D-Allethrin, are widely used for domestic and agricultural purposes and are found to be toxic to many organ systems including the male reproductive system. However, the molecular mechanisms of Allethrin toxicity are not well understood. In this study, we demonstrate that Allethrin exhibited a dose-dependent cytotoxicity on Leydig cell carcinoma cells (LC540) and isolated primary Leydig cells with an IC50 of 125 μM and 59 μM respectively. Cytotoxicity was associated with generation of reactive oxygen species, increased lipid peroxidation and alterations in antioxidant enzyme status. Morphological analyses of LC540 cells treated with Allethrin revealed the presence of apoptotic bodies. Using flow cytometry, we observed that the number of cells that displayed early apoptotic features and entering into G0 phase of cell cycle increased significantly with loss of mitochondrial membrane potential. The levels of p53 mRNA and cleaved PARP-1 protein were increased, whereas BCL-2, pro-Caspase-3 and PARP-1 were decreased. Allethrin induced apoptosis was associated with voltage gated calcium channel mediated intracellular calcium release. Results of our study demonstrate that Allethrin toxicity in the male reproductive tract may involve Leydig cell apoptotic death.

  • Allethrin induced toxicity in the male reproductive tract of rats contributes to disruption in the transcription of genes involved in germ cell production
    Environmental Toxicology, 2013
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Pyrethroids are known to be neurotoxic. However, their toxic effects including that of Allethrin on the male reproductive tract are not elucidated. Adult male rats were treated orally with 25, 50, 100, and 150 mg/kg body weight Allethrin every day for 60 days. Lipid peroxidation was increased (p < 0.001) in the caput, cauda, and testes. Nitric oxide production was increased (p < 0.001) in the caput, but unaltered in the cauda and testes. The activities of catalase, glutathione peroxidase, glutathione-S-transferase, and superoxide dismutase were decreased in the caput and cauda where as a decrease was observed in the testis obtained from Allethrin treated rats. In the epididymides and testes, damage to tubular architecture, congestion, degeneration of epithelial cell lining, intestinal edema, and presence of dead or degenerating spermatids were observed in a dose dependent manner. The expression profile of genes involved in spermatogenesis (Tgf-beta1), sperm maturation (Spag11e), and sperm function (Defb22) were reduced (p < 0.001) in Allethrin rats. The expression of p53 gene was decreased and increased phosphorylation of MAPK (p42/p44) expression was observed the male reproductive tract tissues of Allethrin treated rats. Although earlier studies have reported the effects of Allethrin inhalation because of the use of mosquito coils and vaporizers, our results for the first time prove that oral exposure to Allethrin could affect fertility and may contribute to deregulation of cell cycle in the male reproductive tract. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 1330–1345, 2014.

  • Allethrin induced toxicity in the male reproductive tract of rats contributes to disruption in the transcription of genes involved in germ cell production
    Environmental Toxicology, 2013
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Pyrethroids are known to be neurotoxic. However, their toxic effects including that of Allethrin on the male reproductive tract are not elucidated. Adult male rats were treated orally with 25, 50, 100, and 150 mg/kg body weight Allethrin every day for 60 days. Lipid peroxidation was increased (p 

Abdul Ghofur - One of the best experts on this subject based on the ideXlab platform.

  • pengaruh air kelapa muda cocos nucifera terhadap kualitas spermatozoa mencit mus musculus galur balb c yang terpapar Allethrin sebagai sumber belajar pada mata kuliah fisiologi hewan tesis
    DISERTASI dan TESIS Program Pascasarjana UM, 2013
    Co-Authors: Erik Perdana Putra, Umie Lestari, Abdul Ghofur
    Abstract:

    Plants and animals are often the object used by the people of Indonesia as a traditional medicine to cure various diseases , one example is the coconut . Especially young coconuts , its water has many benefits that can be used as medicine . Young coconut water contains vitamin C 2.2 to 3.4 mg/100 ml , a number of mineral nitrogen , phosphorus , potassium , magnesium , chlorine , sulfur , and iron . Moreover, it also contained micronutrien such as thiamin , riboflavin , folic acid and selenium . Vitamin C and the minerals act as antioxidants to counteract and neutralize free radicals such as allethtrin of mosquito coils and electric. Allethrin is the active compound in the form of synthetic chemical products used in a commercial insecticide derived from natural insecticide pyrethrum which is synthesized from chrysanthemums ( Chrysanthenim cinerariafolium ). This study aims to : (1) to analyze the effects of coconut water ( Cocos nucifera ) on the quality of spermatozoa of mice ( Mus musculus ) strains balb c were exposed to Allethrin , (2) to evaluate and generate research -based module that contains the results of research on the effect of young coconut water ( Cocos nucifera ) on the quality of spermatozoa of mice ( Mus musculus ) strain balb c were exposed to Allethrin that can be used as a learning resource on Animal Physiology Subject . This study uses a quantitative experiment approach the design followed randomized completely design . There are 5 treatment groups and each of the 5 repetition, the experimental group was given the different concentration of coconut water is 25 %, 50% , 75% and 100 % and the control group with aquades for 36 days . Each treatment group had also given exposure to Allethrin of electrical coils for 8 hours per day. Data retrieval is done by chopping cauda epididymis in 0.9% NaCl to form a suspension , and then observed the quality of spermatozoa which includes concentration , morphology , viability and motility . Data were analyzed using one-way analysis of variance ( One Way ANOVA ). The results showed that the effect of coconut water on the quality of spermatozoa of mice previously given exposure to Allethrin . The young coconut water at a concentration of 100% indicates a significant effect in increasing the concentration of spermatozoa . At indicators morphology , viability and motility , young coconut water at a concentration of 50% began to significantly improve the quality of spermatozoa in mice . Based on the results of this study and the relevant literature , compiled a module in the form of teaching materials for the college students in Animal Physiology courses on the subject of Reproductive System . Tanaman dan hewan menjadi objek yang sering dimanfaatkan oleh masyarakat Indonesia sebagai obat-obatan tradisional untuk penyembuhan berbagai macam penyakit, salah satu contoh tanaman tersebut adalah kelapa. Khususnya buah kelapa muda, airnya memiliki banyak khasiat yang dapat digunakan sebagai obat. Air kelapa muda mengandung vitamin C 2,2-3,4 mg/100 ml, sejumlah mineral nitrogen, fosfor, kalium, magnesium, klorin, sulfur, dan besi. Selain itu, juga terkandung micronutrien lain seperti thiamin , riboflavin, asam folat dan selenium. Vitamin C dan mineral-mineral tersebut berperan sebagai antioksidan untuk menangkal dan menetralisir radikal bebas seperti senyawa allethtrin dari obat nyamuk bakar maupun elektrik. Allethrin adalah senyawa aktif berupa produk sintetik dari bahan kimia yang digunakan dalam insektisida komersial yang berasal dari insektisida alami pyrethrum yang disintesis dari bunga krisan ( Chrysanthenim cinerariafolium ). Penelitian ini bertujuan: (1) untuk menganalisis pengaruh pemberian air kelapa muda ( Cocos nucifera ) terhadap kualitas spermatozoa mencit ( Mus musculus ) galur balb c yang terpapar Allethrin , (2) untuk mengevaluasi dan menghasilkan modul berbasis riset yang berisikan hasil penelitian pengaruh air kelapa muda ( Cocos nucifera ) terhadap kualitas spermatozoa mencit ( Mus musculus ) galur balb c yang terpapar Allethrin yang dapat dipergunakan sebagai sumber belajar pada mata kuliah Fisiologi Hewan. Penelitian ini menggunakan pendekatan kuantitatif eksperimen dengan model rancangan acak lengkap (RAL). Terdapat 5 kelompok perlakuan dan masing-masing 5 ulangan, kelompok eksperimen diberikan konsentrasi air kelapa muda yang berbeda yaitu 25%, 50%, 75% dan 100% dan kelompok kontrol dengan aquades selama 36 hari. Setiap kelompok perlakuan sebelumnya juga diberikan paparan Allethrin dari obat nyamuk elektrik selama 8 jam per hari. Pengambilan data dilakukan dengan cara mencacah kauda epididimis di dalam NaCl 0,9% sampai terbentuk suspensi, kemudian diamati kualitas spermatozoa yang meliputi konsentrasi, morfologi, viabilitas dan motilitas. Data yang diperoleh dianalisis dengan menggunakan analisis varian satu jalur ( One Way ANOVA) . Hasil penelitian menunjukkan adanya pengaruh air kelapa muda terhadap kualitas spermatozoa mencit yang sebelumnya diberikan paparan Allethrin . Air kelapa muda pada konsentrasi 100% menunjukkan pengaruh yang signifikan dalam meningkatkan konsentrasi spermatozoa. Pada indikator morfologi, viabilitas dan motilitas, air kelapa muda pada konsentrasi 50% mulai berpengaruh secara signifikan dalam meningkatkan kualitas spermatozoa mencit. Berdasarkan hasil penelitian ini dan kajian pustaka yang relevan, disusun sebuah bahan ajar berupa modul bagi mahasiswa pada mata kuliah Fisiologi Hewan pada pokok bahasan Sistem Reproduksi. Keyword: young coconut water, spermatozoa, Allethrin

Golla Madhubabu - One of the best experts on this subject based on the ideXlab platform.

  • exposure to Allethrin based mosquito coil smoke during gestation and postnatal development affects reproductive function in male offspring of rat
    Inhalation Toxicology, 2017
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    AbstractThe threat of zika virus looms throughout the world and the use of Allethrin-based mosquito coils to prevent mosquito bites during and postpregnancy is on the rise. The aim of this study was to analyze the toxic effects of exposure to Allethrin-based mosquito coil smoke in rats under conditions that reflect human settings. Pregnant female rats were exposed to mosquito coil smoke and same was continued to the male pups up to 111 days postparturition (21-day weaning plus up to 90 days postweaning). Increased oxidative stress, distorted antioxidant enzyme status, downregulation of genes involved in spermatogenesis, sperm maturation and steroidogenesis was observed. Daily sperm production, total sperm count and acrosome reaction was compromised. Results of our study indicate the toxic effects of exposure to Allethrin-based mosquito coil smoke in male offspring and calls for preventing mosquito coil use during pregnancy and postnatal development. Community-based programs that will encourage general pop...

  • Allethrin toxicity causes reproductive dysfunction in male rats
    Environmental Toxicology, 2017
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Pyrethroids are widely used for domestic and agricultural purposes and their use is increasing, especially in developing countries. Uncontrolled use of these insecticides resulted in their entry into the food chain thereby causing toxicity to different organ systems. Allethrin is one of the widely used pyrethroids, but its toxicological effects are underreported when compared to other pyrethroids. Further, its effects on the male reproductive tract remain uncharacterized. In this study, its toxicity on the male reproductive tract was evaluated by administering 25–150 mg/kg body weight Allethrin to adult rats for 60 days. The mRNA expression of factors that are important in spermatogenesis (Scf, c-Kit, Hsf2, Ovol1, Brdt, Kdm3A, Ybx-2, and Grth) and steroidogenesis (StAR, 3β-HSD, 17β-HSD) was significantly downregulated. Decreased levels of testosterone, reduced sperm count and daily sperm production was also observed due to Allethrin toxicity. However, sperm quality parameters assessed by computer-assisted sperm analyzer were not affected. Spermatozoa obtained from Allethrin-treated rats failed to undergo acrosome reaction. Results of this study indicate that Allethrin affects spermatogenesis and sperm function, thus lending further support to the growing evidence of its toxicity.

  • Allethrin induces oxidative stress apoptosis and calcium release in rat testicular carcinoma cells lc540
    Toxicology in Vitro, 2014
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Abstract Over the years, pyrethroids, including D-Allethrin, are widely used for domestic and agricultural purposes and are found to be toxic to many organ systems including the male reproductive system. However, the molecular mechanisms of Allethrin toxicity are not well understood. In this study, we demonstrate that Allethrin exhibited a dose-dependent cytotoxicity on Leydig cell carcinoma cells (LC540) and isolated primary Leydig cells with an IC50 of 125 μM and 59 μM respectively. Cytotoxicity was associated with generation of reactive oxygen species, increased lipid peroxidation and alterations in antioxidant enzyme status. Morphological analyses of LC540 cells treated with Allethrin revealed the presence of apoptotic bodies. Using flow cytometry, we observed that the number of cells that displayed early apoptotic features and entering into G0 phase of cell cycle increased significantly with loss of mitochondrial membrane potential. The levels of p53 mRNA and cleaved PARP-1 protein were increased, whereas BCL-2, pro-Caspase-3 and PARP-1 were decreased. Allethrin induced apoptosis was associated with voltage gated calcium channel mediated intracellular calcium release. Results of our study demonstrate that Allethrin toxicity in the male reproductive tract may involve Leydig cell apoptotic death.

  • Allethrin induced toxicity in the male reproductive tract of rats contributes to disruption in the transcription of genes involved in germ cell production
    Environmental Toxicology, 2013
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Pyrethroids are known to be neurotoxic. However, their toxic effects including that of Allethrin on the male reproductive tract are not elucidated. Adult male rats were treated orally with 25, 50, 100, and 150 mg/kg body weight Allethrin every day for 60 days. Lipid peroxidation was increased (p < 0.001) in the caput, cauda, and testes. Nitric oxide production was increased (p < 0.001) in the caput, but unaltered in the cauda and testes. The activities of catalase, glutathione peroxidase, glutathione-S-transferase, and superoxide dismutase were decreased in the caput and cauda where as a decrease was observed in the testis obtained from Allethrin treated rats. In the epididymides and testes, damage to tubular architecture, congestion, degeneration of epithelial cell lining, intestinal edema, and presence of dead or degenerating spermatids were observed in a dose dependent manner. The expression profile of genes involved in spermatogenesis (Tgf-beta1), sperm maturation (Spag11e), and sperm function (Defb22) were reduced (p < 0.001) in Allethrin rats. The expression of p53 gene was decreased and increased phosphorylation of MAPK (p42/p44) expression was observed the male reproductive tract tissues of Allethrin treated rats. Although earlier studies have reported the effects of Allethrin inhalation because of the use of mosquito coils and vaporizers, our results for the first time prove that oral exposure to Allethrin could affect fertility and may contribute to deregulation of cell cycle in the male reproductive tract. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 1330–1345, 2014.

  • Allethrin induced toxicity in the male reproductive tract of rats contributes to disruption in the transcription of genes involved in germ cell production
    Environmental Toxicology, 2013
    Co-Authors: Golla Madhubabu, Suresh Yenugu
    Abstract:

    Pyrethroids are known to be neurotoxic. However, their toxic effects including that of Allethrin on the male reproductive tract are not elucidated. Adult male rats were treated orally with 25, 50, 100, and 150 mg/kg body weight Allethrin every day for 60 days. Lipid peroxidation was increased (p 

Yulia Nur Hasanah - One of the best experts on this subject based on the ideXlab platform.

  • perbedaan daya hidup nyamuk aedes aegypti setelah dipapar lc50 ekstrak bangle zingiber purpureum dan anti nyamuk cair berbahan aktif d Allethrin dan transflutrin
    Jurnal Kesehatan Masyarakat (e-Journal), 2015
    Co-Authors: Yulia Nur Hasanah, Nur Endah Wahyuningsih, Yusniar D Hanani
    Abstract:

    Dengue hemorrhagic fever is a disease caused by the dengue virus and transmitted by Aedes aegypti. Mostly, mosquito control uses synthetic insecticides that can cause resistance in mosquitoes and pollution in the environment so we can choose alternative natural insecticides from plants such as bangle (Zingiber purpureum) which contain essential oils, saponins, flavonoids, tannins and resins. This study aimed to analyze the difference vitality of Aedes aegypti L. after exposed LC 50 bangle extract (zingiber purpureum Roxb.) and liquid mosquito insecticides d-Allethrin and transflutrin. This study was a true experiment with post test only group design used Aedes aegypti population with aged 2-5 days are reared in B2P2VRP Laboratory in Salatiga, Central Java and use 900 mosquitoes for samples. Results of probit analysis LC 50 of bangle extract was 660.000 ppm, d-Allethrin was 36 ppm and transfluthrin was 27 ppm. The study showed difference of longevity Aedes aegypti among control and exposed group bangle, bangle and d-Allethrin, bangle and transfluthrin (p = 0.0001)(Post Hoc, Tukey). There was no difference of  Aedes aegypti longevity among the control, d-Allethrin (p = 0.074) and transfluthrin (p = 0.999), moreover there was also no difference of Aedes aegypti longevity between  exposed group d-Allethrin and transfluthrin (p = 0.094). The result showed no difference the number of surviving Aedes aegypti among control, exposed group of bangle, d-Allethrin and transfluthrin (p = 0,607) (Kruskal Wallis).

  • perbedaan daya hidup nyamuk aedes aegypti l setelah dipapar lc50 ekstrak bangle zingiber purpureum roxb dan anti nyamuk cair berbahan aktif d Allethrin dan transfluthrin
    2014
    Co-Authors: Yulia Nur Hasanah
    Abstract:

    Demam Berdarah Dengue merupakan penyakit yang disebabkan oleh virus dengue dan disebarkan oleh nyamuk Aedes aegypti. Pengendalian nyamuk paling banyak menggunakan insektisida sintetis yang menyebabkan resistensi pada nyamuk dan pencemaran lingkungan. Alternatif lain adalah penggunaan insektisida nabati yang berasal dari tumbuhan seperti bangle (Zingiber purpureum) yang mengandung minyak atsiri, saponin, flavonoid, tanin dan damar. Penelitian ini bertujuan menganalisis perbedaan daya hidup nyamuk Aedes aegypti setelah dipapar LC50 ekstrak bangle dan anti nyamuk cair berbahan aktif d-Allethrin dan transfluthrin. Penelitian ini merupakan true experiment dengan post test only group design menggunakan populasi nyamuk Aedes aegypti berumur 2-5 hari yang dipelihara di Laboratorium B2P2VRP Salatiga, Jawa Tengah dengan jumlah sampel sebanyak 900 ekor nyamuk. Hasil analisis probit diperoleh LC50 sebesar 660.000 ppm untuk ekstrak bangle, 36 ppm untuk d-Allethrin dan 27 ppm untuk transfluthrin. Hasil penelitian menunjukkan adanya perbedaan bermakna lama hidup nyamuk Aedes aegypti antara kelompok kontrol dengan kelompok bangle, kelompok bangle dengan kelompok d-Allethrin dan kelompok bangle dengan kelompok transfluthrin (0,0001).(Post hoc, Tukey) Lama hidup nyamuk Aedes aegypti /i> pada kelompok kontrol tidak ada perbedaan bermakna dengan lama hidup nyamuk Aedes aegypti pada kelompok d-Allethrin (p = 0,074) dan transfluthrin (p = 0,999). Selain itu tidak ada perbedaan bermakna juga pada lama hidup nyamuk Aedes aegypti pada kelompok d-Allethrin dan transfluthrin (p = 0,094). Hasil uji beda jumlah nyamuk survive menunjukkan tidak ada beda jumlah nyamuk Aedes aegypti survive pada kelompok kontrol dan kelompok terpapar LC50 ekstrak bangle, obat nyamuk cair d-Allethrin dan transfluthrin (p = 0,607) (Kruskal Wallis). Kata Kunci: Aedes aegypti, ekstrak bangle, d-Allethrin, transfluthrin, daya hidup

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