The Experts below are selected from a list of 114 Experts worldwide ranked by ideXlab platform
Taigyu Kim - One of the best experts on this subject based on the ideXlab platform.
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comprehensive analysis of cd4 t cell responses to cmv pp65 antigen restricted by single hla dr dq and dp Allotype within an individual
Frontiers in Immunology, 2021Co-Authors: Youseok Hyun, Yonghun Lee, Sunmi Kim, Incheol Baek, Hyunjung Sohn, Hyunil Cho, Taigyu KimAbstract:Within an individual, six different HLA class II heterodimers are expressed co-dominantly by two alleles of HLA-DR, -DQ, and -DP loci. However, it remained unclear which HLA Allotypes were used in T cell responses to a given antigen. For the measurement of the CD4+ T cell responses restricted by a single HLA Allotype, we established a panel of artificial antigen-presenting cells (aAPCs) expressing each single HLA allele of 20 HLA-DRB1, 16 HLA-DQ, and 13 HLA-DP alleles. CD4+ T cell responses to cytomegalovirus (CMV) pp65 restricted by single HLA class II Allotype defined in 45 healthy volunteers. The average magnitude of CD4+ T cell responses by HLA-DR Allotypes was higher than HLA-DQ and HLA-DP Allotypes. CD4+ T cell responses by DRA*01:01/DRB1*04:06, DQA1*01:02/DQB1*06:02, DPA1*02:02/DPB1*05:01 were higher among the other alleles in each HLA-DR, -DQ, and -DP locus. Interestingly, the frequencies of HLA-DR alleles and the positivity of specific Allotypes showed an inverse correlation. One Allotype within individuals is dominantly used in CD4+ T cell response in 49% of donors, and two Allotypes showed that in 7% of donors, and any positive response was detected in 44% of donors. Even if one individual had several dominant alleles, CD4+ T cell responses tended to be responded by only one of them. Furthermore, CD8+ and CD4+ T cell responses by HLA class I and class II were correlated. Our results demonstrate that the CD4+ T cell immune response to pp65 preferentially uses a few dominant HLA class II Allotypes within individuals similar to HLA class I.
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comprehensive analysis of cytomegalovirus pp65 antigen specific cd8 t cell responses according to human leukocyte antigen class i Allotypes and intraindividual dominance
Frontiers in Immunology, 2017Co-Authors: Seungjoo Hyun, Hyunjung Sohn, Hyunil Cho, Hyunjoo Lee, Seonduk Lee, Sueon Kim, Daehee Sohn, Cheolhwa Hong, Haeyoun Choi, Taigyu KimAbstract:To define whether individual human leukocyte antigen (HLA) class I Allotypes are used preferentially in human cytomegalovirus (CMV)-specific cytotoxic T lymphocyte responses, CD8+ T cell responses restricted by up to six HLA class I Allotypes in an individual were measured in parallel using K562-based artificial antigen-presenting cells expressing both CMV pp65 antigen and one of 32 HLA class I Allotypes (7 HLA-A, 14 HLA-B, and 11 HLA-C) present in 50 healthy Korean donors. The CD8+ T cell responses to pp65 in the HLA-C Allotypes were lower than responses to those in HLA-A and -B Allotypes and there was no difference between the HLA-A and HLA-B loci. HLA-A*02:01, -B*07:02, and -C*08:01 showed the highest magnitude and frequency of immune responses to pp65 at each HLA class I locus. However, HLA-A*02:07, -B*59:01, -B*58:01, -B*15:11, -C*03:02, and -C*02:02 did not show any immune responses. Although each individual has up to six different HLA Allotypes, 46% of the donors showed one Allotype, 24% showed two Allotypes, and 2% showed three Allotypes that responded to pp65. Interestingly, the frequencies of HLA-A alleles were significantly correlated with the positivity of specific Allotypes. Our results demonstrate that specific HLA class I Allotypes are preferentially used in the CD8+ T cell immune response to pp65 and that a hierarchy among HLA class I Allotypes is present in an individual.
Masaki Takeuchi - One of the best experts on this subject based on the ideXlab platform.
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a single endoplasmic reticulum aminopeptidase 1 protein Allotype is a strong risk factor for behcet s disease in hla b 51 carriers
Annals of the Rheumatic Diseases, 2016Co-Authors: Masaki Takeuchi, Michael J Ombrello, Yohei Kirino, Burak Erer, Ilknur Tugaltutkun, Emire Seyahi, Yilmaz Ozyazgan, Norman R Watts, Ahmet GulAbstract:Introduction Endoplasmic reticulum aminopeptidase-1 (ERAP1) protein is highly polymorphic with numerous missense amino acid variants. We sought to determine the naturally occurring ERAP1 protein Allotypes and their contribution to Behcet9s disease. Methods Genotypes of all reported missense ERAP1 gene variants with 1000 Genomes Project EUR superpopulation frequency >1% were determined in 1900 Behcet9s disease cases and 1779 controls from Turkey. ERAP1 protein Allotypes and their contributions to Behcet9s disease risk were determined by haplotype identification and disease association analyses. Results One ERAP1 protein Allotype with five non-ancestral amino acids was recessively associated with disease (p=3.13×10 −6 , OR 2.55, 95% CI 1.70 to 3.82). The ERAP1 association was absent in individuals who lacked HLA-B*51. Individuals who carry HLA-B*51 and who are also homozygous for the haplotype had an increased disease odds compared with those with neither risk factor (p=4.80×10 −20 , OR 10.96, 95% CI 5.91 to 20.32). Discussion The Behcet9s disease-associated ERAP1 protein Allotype was previously shown to have poor peptide trimming activity. Combined with its requirement for HLA-B*51, these data suggest that a hypoactive ERAP1 Allotype contributes to Behcet9s disease risk by altering the peptides available for binding to HLA-B*51.
Niels Morling - One of the best experts on this subject based on the ideXlab platform.
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plasma vitamin d binding protein gc factors immunoglobulin g heavy chain gm Allotypes and immunoglobulin kappa light chain km1 Allotype in patients with sarcoidosis and in healthy control subjects
Sarcoidosis Vasculitis and Diffuse Lung Diseases, 2002Co-Authors: Nils Milman, Mariann Thymann, Niels Graudal, Niels MorlingAbstract:BACKGROUND AND AIM: Sarcoidosis is an immune disease with abnormalities in the production of vitamin D and immunoglobulins. The aim was to examine whether the distribution of plasma vitamin D-binding protein = group-specific component (GC) Allotypes, immunoglobulin G heavy chain (GM) Allotypes and immunoglobulin kappa light chain (KM) Allotype differed significantly from the distribution in healthy subjects. METHODS: GC 1S, 1F, 2 Allotypes, GM 1, 2, 5 Allotypes, and KM1 Allotype were assessed in 44 patients with sarcoidosis and in healthy control subjects. RESULTS: There were no significant differences between the frequencies of the GC, GM and KM Allotypes in sarcoidosis patients and in control subjects. Furthermore, there was no relationship between the presentation or course of the sarcoid disease and GC, GM or KM Allotypes. CONCLUSIONS: GC, GM and KMI Allotypes do not appear to play any major role in the pathogenesis of sarcoidosis.
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plasma vitamin d binding protein gc factors immunoglobulin g heavy chain gm Allotypes and immunoglobulin kappa light chain km1 Allotype in patients with sarcoidosis and in healthy control subjects
Sarcoidosis Vasculitis and Diffuse Lung Diseases, 2002Co-Authors: Nils Milman, Mariann Thymann, Niels Graudal, Niels MorlingAbstract:BACKGROUND AND AIM Sarcoidosis is an immune disease with abnormalities in the production of vitamin D and immunoglobulins. The aim was to examine whether the distribution of plasma vitamin D-binding protein = group-specific component (GC) Allotypes, immunoglobulin G heavy chain (GM) Allotypes and immunoglobulin kappa light chain (KM) Allotype differed significantly from the distribution in healthy subjects. METHODS GC 1S, 1F, 2 Allotypes, GM 1, 2, 5 Allotypes, and KM1 Allotype were assessed in 44 patients with sarcoidosis and in healthy control subjects. RESULTS There were no significant differences between the frequencies of the GC, GM and KM Allotypes in sarcoidosis patients and in control subjects. Furthermore, there was no relationship between the presentation or course of the sarcoid disease and GC, GM or KM Allotypes. CONCLUSIONS GC, GM and KMI Allotypes do not appear to play any major role in the pathogenesis of sarcoidosis.
Janardan P. Pandey - One of the best experts on this subject based on the ideXlab platform.
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Genetic interactions of KIR and G1M immunoglobulin Allotypes differ in obese from non-obese individuals with type 2 diabetes.
Molecular immunology, 2008Co-Authors: Viviana Romero, Janardan P. Pandey, Joaquín Zúñiga, Jose Azocar, Olga P. Clavijo, Daniel Terreros, Hassan Kidwai, Edmond J. YunisAbstract:We analyzed the natural killer cell immunoglobulin-like receptor (KIR) genes and immunoglobulin Allotypes in the development of type 2 diabetes (T2D) based on body mass index (BMI) measurements (obese vs. non-obese) in Puerto Rican Americans. Genetic interactions between the KIR haplotype A homozygotes (HAH) and its fraction containing two inhibitory receptors 2DL3 and 2DL1 and the activating receptor 2DS4 with immunoglobulin Allotypes were studied. We found a significant association between the HAH and T2D (p = 0.002; OR = 7.97) and its interaction with the immunoglobulin Allotype z: GM f/f (−) (p =
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Immunoglobulin GM and KM Allotypes and vaccine immunity.
Vaccine, 2000Co-Authors: Janardan P. PandeyAbstract:Abstract Immunoglobulin (Ig) GM and KM Allotypes are genetic markers of γ and κ-type light chains, respectively. The striking qualitative and quantitative differences in the distribution of these determinants among different races raise questions concerning the nature of the evolutionary selective mechanism that maintains this variation. Associations between Ig Allotypes and specific antibody responses could be a selective force for the maintenance of various haplotypes and their frequencies. Data from several studies reporting significant associations between certain GM and KM Allotypes and immune responsiveness to polysaccharide vaccines and to particular infectious pathogens support this hypothesis. Possible ways in which constant (C)-region Allotypes could contribute to the antibody specificity include the following: (i) certain alleles coding for Allotypes may be in linkage disequilibrium with particular variable (V)-region determinants associated with immune responsiveness; (ii) they could directly contribute to the formation of specific idiotypes, as shown for the T15 system in mice; and (iii) Allotype-associated structural variability in the C-region could modulate the kinetic competence of the antigen binding sites.
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immunoglobulin Allotype gene polymorphisms in systemic sclerosis interactive effect of mhc class ii and km genes on anticentromere antibody production
Annals of the Rheumatic Diseases, 1998Co-Authors: Hideto Kameda, Janardan P. Pandey, Junichi Kaburaki, Hidetoshi Inoko, Masataka KuwanaAbstract:Objective—To examine potential interactions between immunoglobulin (Ig)Allotype gene polymorphisms and susceptibility to systemic sclerosis (SSc) as well as serological expression in SSc patients. Methods—IgG heavy chain Allotypes G1M(f, z), G2M(n+, n-), G3M(b, g) and Ig light chain Allotype KM(1, (1, 2), 3) were genotyped in 105 Japanese SSc patients and 47 race matched normal controls using polymerase chain reaction (PCR) based methods. Associations of each Ig Allotype with SSc related antinuclear antibodies were examined in combination with or without MHC class II alleles. Results—GM/KM genotypic and allelic frequencies were similar in SSc patients and in normal controls. Frequencies of G1M(f) and G2M(n+) were significantly decreased in anticentromere antibody (ACA) positive SSc patients compared with ACA negative SSc patients (p = 0.04 and 0.02, respectively). Conversely, the presence of DQB1*0501 and KM(1, 2) significantly increased the risk of ACA positivity. Conclusion—Ig Allotype gene polymorphisms were not associated with susceptibility to SSc.Instead,the results suggested that MHC class II and KM genes are associated with autoimmune responses by interactively promoting the production of ACA. (Ann Rheum Dis 1998;57:366‐370)
Ahmet Gul - One of the best experts on this subject based on the ideXlab platform.
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a single endoplasmic reticulum aminopeptidase 1 protein Allotype is a strong risk factor for behcet s disease in hla b 51 carriers
Annals of the Rheumatic Diseases, 2016Co-Authors: Masaki Takeuchi, Michael J Ombrello, Yohei Kirino, Burak Erer, Ilknur Tugaltutkun, Emire Seyahi, Yilmaz Ozyazgan, Norman R Watts, Ahmet GulAbstract:Introduction Endoplasmic reticulum aminopeptidase-1 (ERAP1) protein is highly polymorphic with numerous missense amino acid variants. We sought to determine the naturally occurring ERAP1 protein Allotypes and their contribution to Behcet9s disease. Methods Genotypes of all reported missense ERAP1 gene variants with 1000 Genomes Project EUR superpopulation frequency >1% were determined in 1900 Behcet9s disease cases and 1779 controls from Turkey. ERAP1 protein Allotypes and their contributions to Behcet9s disease risk were determined by haplotype identification and disease association analyses. Results One ERAP1 protein Allotype with five non-ancestral amino acids was recessively associated with disease (p=3.13×10 −6 , OR 2.55, 95% CI 1.70 to 3.82). The ERAP1 association was absent in individuals who lacked HLA-B*51. Individuals who carry HLA-B*51 and who are also homozygous for the haplotype had an increased disease odds compared with those with neither risk factor (p=4.80×10 −20 , OR 10.96, 95% CI 5.91 to 20.32). Discussion The Behcet9s disease-associated ERAP1 protein Allotype was previously shown to have poor peptide trimming activity. Combined with its requirement for HLA-B*51, these data suggest that a hypoactive ERAP1 Allotype contributes to Behcet9s disease risk by altering the peptides available for binding to HLA-B*51.
