The Experts below are selected from a list of 36783 Experts worldwide ranked by ideXlab platform

Claudia Soto - One of the best experts on this subject based on the ideXlab platform.

  • effect of silymarin on kidneys of rats suffering from alloxan induced Diabetes Mellitus
    Phytomedicine, 2010
    Co-Authors: Claudia Soto, Julia Perez, V Garcia, E Uria, M Vadillo, L Raya
    Abstract:

    Oxidative stress contributes to the pathogenesis of Diabetes Mellitus and its sequelae nephropathy. The kidneys are especially prone to damage by free radicals. We therefore tested the effect of the flavonoid mixture silymarin, a free radical scavenger, on the activity and gene expression of superoxide dismutase, glutathione peroxidase and catalase, as well as on renal tissue morphology in rats with Alloxan-Induced Diabetes Mellitus. Alloxan-intoxicated rats were treated with silymarin 20 days after alloxan administration for 9 weeks. Alloxan-Induced tissue damage and decreased the activity of the three enzymes, SOD (U/mg prot.): 14.4±1.75 vs 112±6.45 control, p<0.05, n=6; GSHPx (μM NADPH/min/mg prot.): 0.02±0.002 vs 0.121±0.01 control, p<0.05, n=6; CAT (k/seg/mg prot.): 0.022±0.003 vs 0.044±0.002 control, p<0.05, n=6. Silymarin treatment prevented tissue damage and restored the activity (SOD: 110.7±12.9U/mg prot.; GSHPx: 0.329±0.031 μM NADPH/min/mg prot.; CAT: 0.054±0.002 k/seg/mg prot., n=6) and gene expression of the three antioxidant enzymes after 20 days of alloxan administration (SOD: 12.00±0.57 control, 9.00±0.1 diabetic p<0.05, 11.00±0.20 silymarin treated; GSHPx: 6.01±0.78 control, 9.03±0.3 diabetic p<0.05, 7.02±0.07 silymarin treated; CAT: 9.03±1.07 control, 12.02±0.60 diabetic p<0.05, 8.06±0.31 silymarin treated, n=6). It is suggested in this study that recuperative effect of silymarin on the renal tissue damage induced by alloxan may be related to an increase in the activity and recovery of gene expression of antioxidant enzymes which in addition to the glutathione system constitute some of the most important defense mechanisms against free radicals damage. As these results show, silymarin may be considered potentially in the treatment of diabetic nephropathy.

  • effect of silymarin on kidneys of rats suffering from alloxan induced Diabetes Mellitus
    Phytomedicine, 2010
    Co-Authors: Claudia Soto, Julia Perez, V Garcia, E Uria, M Vadillo, L Raya
    Abstract:

    Abstract Oxidative stress contributes to the pathogenesis of Diabetes Mellitus and its sequelae nephropathy. The kidneys are especially prone to damage by free radicals. We therefore tested the effect of the flavonoid mixture silymarin, a free radical scavenger, on the activity and gene expression of superoxide dismutase, glutathione peroxidase and catalase, as well as on renal tissue morphology in rats with Alloxan-Induced Diabetes Mellitus. Alloxan-intoxicated rats were treated with silymarin 20 days after alloxan administration for 9 weeks. Alloxan-Induced tissue damage and decreased the activity of the three enzymes, SOD (U/mg prot.): 14.4 ± 1.75 vs 112 ± 6.45 control, p n  = 6; GSHPx (μM NADPH/min/mg prot.): 0.02 ± 0.002 vs 0.121 ± 0.01 control, p n  = 6; CAT (k/seg/mg prot.): 0.022 ± 0.003 vs 0.044 ± 0.002 control, p n  = 6. Silymarin treatment prevented tissue damage and restored the activity (SOD: 110.7 ± 12.9 U/mg prot.; GSHPx: 0.329 ± 0.031 μM NADPH/min/mg prot.; CAT: 0.054 ± 0.002 k/seg/mg prot., n  = 6) and gene expression of the three antioxidant enzymes after 20 days of alloxan administration (SOD: 12.00 ± 0.57 control, 9.00 ± 0.1 diabetic p p p n  = 6). It is suggested in this study that recuperative effect of silymarin on the renal tissue damage induced by alloxan may be related to an increase in the activity and recovery of gene expression of antioxidant enzymes which in addition to the glutathione system constitute some of the most important defense mechanisms against free radicals damage. As these results show, silymarin may be considered potentially in the treatment of diabetic nephropathy.

  • silymarin increases antioxidant enzymes in alloxan induced Diabetes in rat pancreas
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2003
    Co-Authors: Claudia Soto, Rosa Recoba, Hector Barron, Carlos Angulo Alvarez, Liliana Favari
    Abstract:

    The aim of this study was to analyze the effect of the flavonoid silymarin, a free radical scavenger that prevents lipoperoxidation, on the pancreatic activity of superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase (CAT) in rats with Alloxan-Induced Diabetes Mellitus. Alloxan intoxicated rats were treated with silymarin in two manners, simultaneously (four or eight doses) or 20 days after alloxan administration for 9 weeks. Alloxan elicited a transient increase in the activity of the three enzymes, which decreased after 5 days of treatment. On its own, silymarin significantly increased the activity of these enzymes. Simultaneous treatment with alloxan and silymarin also induced an increment in the activity of the enzymes followed by a delayed decrease (four doses). However, a longer treatment with silymarin (eight doses) induced a more sustained effect. Interestingly, silymarin treatment recovered to control values for the activity of the three-antioxidant enzymes that were significantly diminished after 20 days of alloxan administration. It is suggested that the protective effect of silymarin on pancreatic damage induced by alloxan may be due to an increase in the activity of antioxidant enzymes that, in addition to the glutathione system, constitute the more important defense mechanisms against damage by free radicals.

L Raya - One of the best experts on this subject based on the ideXlab platform.

  • effect of silymarin on kidneys of rats suffering from alloxan induced Diabetes Mellitus
    Phytomedicine, 2010
    Co-Authors: Claudia Soto, Julia Perez, V Garcia, E Uria, M Vadillo, L Raya
    Abstract:

    Abstract Oxidative stress contributes to the pathogenesis of Diabetes Mellitus and its sequelae nephropathy. The kidneys are especially prone to damage by free radicals. We therefore tested the effect of the flavonoid mixture silymarin, a free radical scavenger, on the activity and gene expression of superoxide dismutase, glutathione peroxidase and catalase, as well as on renal tissue morphology in rats with Alloxan-Induced Diabetes Mellitus. Alloxan-intoxicated rats were treated with silymarin 20 days after alloxan administration for 9 weeks. Alloxan-Induced tissue damage and decreased the activity of the three enzymes, SOD (U/mg prot.): 14.4 ± 1.75 vs 112 ± 6.45 control, p n  = 6; GSHPx (μM NADPH/min/mg prot.): 0.02 ± 0.002 vs 0.121 ± 0.01 control, p n  = 6; CAT (k/seg/mg prot.): 0.022 ± 0.003 vs 0.044 ± 0.002 control, p n  = 6. Silymarin treatment prevented tissue damage and restored the activity (SOD: 110.7 ± 12.9 U/mg prot.; GSHPx: 0.329 ± 0.031 μM NADPH/min/mg prot.; CAT: 0.054 ± 0.002 k/seg/mg prot., n  = 6) and gene expression of the three antioxidant enzymes after 20 days of alloxan administration (SOD: 12.00 ± 0.57 control, 9.00 ± 0.1 diabetic p p p n  = 6). It is suggested in this study that recuperative effect of silymarin on the renal tissue damage induced by alloxan may be related to an increase in the activity and recovery of gene expression of antioxidant enzymes which in addition to the glutathione system constitute some of the most important defense mechanisms against free radicals damage. As these results show, silymarin may be considered potentially in the treatment of diabetic nephropathy.

  • effect of silymarin on kidneys of rats suffering from alloxan induced Diabetes Mellitus
    Phytomedicine, 2010
    Co-Authors: Claudia Soto, Julia Perez, V Garcia, E Uria, M Vadillo, L Raya
    Abstract:

    Oxidative stress contributes to the pathogenesis of Diabetes Mellitus and its sequelae nephropathy. The kidneys are especially prone to damage by free radicals. We therefore tested the effect of the flavonoid mixture silymarin, a free radical scavenger, on the activity and gene expression of superoxide dismutase, glutathione peroxidase and catalase, as well as on renal tissue morphology in rats with Alloxan-Induced Diabetes Mellitus. Alloxan-intoxicated rats were treated with silymarin 20 days after alloxan administration for 9 weeks. Alloxan-Induced tissue damage and decreased the activity of the three enzymes, SOD (U/mg prot.): 14.4±1.75 vs 112±6.45 control, p<0.05, n=6; GSHPx (μM NADPH/min/mg prot.): 0.02±0.002 vs 0.121±0.01 control, p<0.05, n=6; CAT (k/seg/mg prot.): 0.022±0.003 vs 0.044±0.002 control, p<0.05, n=6. Silymarin treatment prevented tissue damage and restored the activity (SOD: 110.7±12.9U/mg prot.; GSHPx: 0.329±0.031 μM NADPH/min/mg prot.; CAT: 0.054±0.002 k/seg/mg prot., n=6) and gene expression of the three antioxidant enzymes after 20 days of alloxan administration (SOD: 12.00±0.57 control, 9.00±0.1 diabetic p<0.05, 11.00±0.20 silymarin treated; GSHPx: 6.01±0.78 control, 9.03±0.3 diabetic p<0.05, 7.02±0.07 silymarin treated; CAT: 9.03±1.07 control, 12.02±0.60 diabetic p<0.05, 8.06±0.31 silymarin treated, n=6). It is suggested in this study that recuperative effect of silymarin on the renal tissue damage induced by alloxan may be related to an increase in the activity and recovery of gene expression of antioxidant enzymes which in addition to the glutathione system constitute some of the most important defense mechanisms against free radicals damage. As these results show, silymarin may be considered potentially in the treatment of diabetic nephropathy.

Anand Kar - One of the best experts on this subject based on the ideXlab platform.

  • protective role of three vegetable peels in alloxan induced Diabetes Mellitus in male mice
    Plant Foods for Human Nutrition, 2010
    Co-Authors: Yamini Dixit, Anand Kar
    Abstract:

    The hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated. In a preliminary study, effects of ethanolic extracts of Cucurbita pepo, Cucumis sativus and Praecitrullus fistulosus peels were studied at 250 and 500 mg kg−1 d−1 for 15 days in the alterations in serum glucose and in hepatic lipid peroxidation (LPO) in male mice. In the pilot experiment, the effective and safe concentration of each peel was administered (p.o.) for 10 consecutive days and then on 11th and 12th days alloxan was administered along with peel extracts. The treatment was continued up to 15th day. At the end, alterations in serum glucose, insulin, triiodothyronine, thyroxine, total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, very low density lipoprotein, hepatic lipid peroxidation, superoxide dismutase and catalase were studied. All the three peel extracts nearly reversed most of these changes induced by alloxan suggesting their possible role in ameliorating Diabetes Mellitus and related changes in serum lipids. However, Cucurbita pepo peel was found to be the most effective. Total polyphenols, flavonoids and ascorbic acid contents of the test peels were also estimated, which appear to be associated with the observed antidiabetic and antioxidative potentials.

  • antidiabetic potential of citrus sinensis and punica granatum peel extracts in alloxan treated male mice
    Biofactors, 2007
    Co-Authors: Hamendra Singh Parmar, Anand Kar
    Abstract:

    An investigation on the effects of four different concentrations of peel extract from Citrus sinensis (CS) or Punica granatum (PG) in male mice revealed the maximum glucose lowering and antiperoxidative activities at 25 mg/kg of CS and 200 mg/kg of PG. In a separate experiment their potential was evaluated with respect to the regulation of alloxan induced Diabetes Mellitus. While a single dose of alloxan (120 mg/kg) increased the serum levels of glucose and α-amylase activity, rate of water consumption and lipid peroxidation (LPO) in hepatic, cardiac and renal tissues with a parallel decrease in serum insulin level, administration of 25 mg/kg of CS or 200 mg/kg of PG was found to normalize all the adverse changes induced by alloxan, revealing the antidiabetic and anti peroxidative potential of test fruit peel extracts. Subsequent phytochemical analysis indicated that the high content of total polyphenols in the test peels might be related to the antidiabetic and antiperoxidative effects of the test peels.

Liliana Favari - One of the best experts on this subject based on the ideXlab platform.

  • silymarin increases antioxidant enzymes in alloxan induced Diabetes in rat pancreas
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2003
    Co-Authors: Claudia Soto, Rosa Recoba, Hector Barron, Carlos Angulo Alvarez, Liliana Favari
    Abstract:

    The aim of this study was to analyze the effect of the flavonoid silymarin, a free radical scavenger that prevents lipoperoxidation, on the pancreatic activity of superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase (CAT) in rats with Alloxan-Induced Diabetes Mellitus. Alloxan intoxicated rats were treated with silymarin in two manners, simultaneously (four or eight doses) or 20 days after alloxan administration for 9 weeks. Alloxan elicited a transient increase in the activity of the three enzymes, which decreased after 5 days of treatment. On its own, silymarin significantly increased the activity of these enzymes. Simultaneous treatment with alloxan and silymarin also induced an increment in the activity of the enzymes followed by a delayed decrease (four doses). However, a longer treatment with silymarin (eight doses) induced a more sustained effect. Interestingly, silymarin treatment recovered to control values for the activity of the three-antioxidant enzymes that were significantly diminished after 20 days of alloxan administration. It is suggested that the protective effect of silymarin on pancreatic damage induced by alloxan may be due to an increase in the activity of antioxidant enzymes that, in addition to the glutathione system, constitute the more important defense mechanisms against damage by free radicals.

  • prevention of alloxan induced Diabetes Mellitus in the rat by silymarin
    Comparative Biochemistry and Physiology Part C: Pharmacology Toxicology and Endocrinology, 1998
    Co-Authors: Claudia P Soto, Liliana Favari, Blanca Estela Lopez Perez, Jose L Reyes
    Abstract:

    Abstract Silymarin is a free-radical scavenger and a membrane stabilizer which prevents lipoperoxidation and its associated cell damage in some experimental models. It has been proposed that lipid peroxidation caused by free radicals may be involved in Alloxan-Induced Diabetes Mellitus. Alloxan elicits pancreatic lipid peroxidation which precedes the appearance of hyperglycemia in mice. We studied the effects of silymarin on rat pancreas, the effect of this flavonoid on pancreatic, hepatic and blood glutathione (GSH) together with the pancreatic malondialdehyde concentrations in response to alloxan. On its own, silymarin increases pancreatic and blood GSH without changes in either hepatic GSH or in blood glucose. Silymarin prevents the increase in lipid peroxidation produced by alloxan. It also blunts the sustained increment in plasma glucose induced by alloxan. We suggest that silymarin has a protective effect on the pancreatic damage in experimental Diabetes Mellitus. This may be related to its antioxidative properties and to the increase in concentrations of plasma and pancreatic glutathione.

Wan G Shin - One of the best experts on this subject based on the ideXlab platform.

  • pharmacokinetics and pharmacodynamics of azosemide after intravenous and oral administration to rats with alloxan induced Diabetes Mellitus
    Journal of Pharmacy and Pharmacology, 1996
    Co-Authors: Kwang J Park, Woo H Yoon, Wan G Shin
    Abstract:

    Because physiological changes occurring in Diabetes Mellitus patients could alter the pharmacokinetics and pharmacodynamics of the drugs used to treat the disease, the pharmacokinetics and pharmacodynamics of azosemide were investigated after intravenous and oral administration of the drug (10 mg kg -1 ) to control and Alloxan-Induced Diabetes Mellitus rats (AIDRs). After intravenous administration of azosemide to the AIDRs, the area under the plasma concentration-time curve (AUC) increased considerably (3120 compared with 2520 μg min mL -1 ; P < 0.135) and the total body clearance decreased considerably (3.20 compared with 3.96 mL min -1 kg -1 ; P < 0.0593). The considerable reduction in time-averaged total body clearance in the AIDRs was a result of the significant decrease in renal clearance (1.01 compared with 1.55 mL min -1 kg -1 ) in the AIDRs, the non-renal clearance being comparable between the two groups of rats. After intravenous administration, the 8-h urinary excretion of azosemide (29.5 compared with 40% of intravenous dose ; P < 0.0883) and one of its metabolites, M1 (2.15 compared with 2.60% of intravenous dose, expressed in terms of azosemide ; P < 0.05) decreased in the AIDRs because of the impaired kidney function. The diuretic, natriuretic, kaliuretic and chloruretic efficiencies increased significantly in the AIDRs. After oral administration of azosemide, AUC decreased significantly in the AIDRs (115 compared with 215 μg min mL -1 ) possibly because of the reduced gastrointestinal absorption of azosemide in the AIDRs. After oral administration of azosemide, the 8-h urine output decreased significantly in the AIDRs (9.32 compared with 16.1 mL per 100 g body weight) because of the significantly reduced 8-h urinary excretion of azosemide (3.00 compared with 9.14% of oral dose). After both intravenous and oral administration some pharmacokinetic and pharmacodynamic parameters of azosemide were significantly different in AIDRs.