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Isabel C.f.r. Ferreira - One of the best experts on this subject based on the ideXlab platform.
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Effects of gamma radiation on the bioactivity of medicinal and aromatic plants: Mentha × piperita L., Thymus vulgaris L. and Aloysia citrodora Paláu as case studies.
Food & function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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effects of gamma radiation on the bioactivity of medicinal and aromatic plants mentha piperita l thymus vulgaris l and Aloysia citrodora palau as case studies
Food & Function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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exploring reserve lots of cymbopogon citratus Aloysia citrodora and thymus citriodorus as improved sources of phenolic compounds
Food Chemistry, 2018Co-Authors: Ingride Rita, Carla Pereira, Lillian Barros, Isabel C.f.r. FerreiraAbstract:Abstract Given the increasing consumers demand for novelty, tea companies have been presenting new added value products such as reserve lots of aromatic plants. Herein, infusions from different lots of three aromatic plants were assessed in terms of phenolic composition (HPLC-DAD-ESI/MS) and antioxidant properties (reducing power, free radical scavenging and lipid peroxidation inhibition capacity). Cymbopogon citratus (C. citratus; main compound 5-O-caffeoylquinic acid) and Aloysia citrodora (A. citrodora; prevalence of verbascoside) reserve lots revealed higher phenolic compounds concentration than the respective standard lots. Thymus × citriodorus (T. citriodorus; main compound rosmarinic acid) standard lot presented higher amounts of phenolic acids than the reserve lot, nonetheless, total flavonoids and phenolic compounds were not significantly different. The differences between both lots antioxidant activity were more noticeable in C. citratus, with the reserve lot presenting the highest activity. This study provides evidence of the differences between these plants chemical composition and bioactivity depending on the harvesting conditions.
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Evaluation of gamma-irradiated aromatic herbs: Chemometric study of samples submitted to extended storage periods.
Food research international (Ottawa Ont.), 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, João C.m. Barreira, M. Beatriz P.p. Oliveira, Isabel C.f.r. FerreiraAbstract:Abstract The preserving capacity of gamma radiation (10 kGy) on the chemical, nutritional and antioxidant components of Aloysia citrodora Palau, Melissa officinalis L., Melittis melissophyllum L. and Mentha piperita L., stored for 12 and 18 months, was evaluated. Despite the maintenance of the main characteristics during the first 12 months of storage, the additional 6 months induced several significant changes in individual compounds. In general, the analyzed species reacted dissimilarly throughout time, but it was possible to verify that the fatty acids, tocopherols and antioxidant capacity presented the most significant changes after 18 months of storage, inclusively in samples submitted to gamma radiation. In fact, the applied treatment (10 kGy) did not seem to be effective to prevent the decrease of free sugars, organic acids and tocopherols, especially considering the 18 months period. On the other hand, the evolution in color parameters indicated a greener color (yet slightly more yellow) among irradiated samples. Likewise, gamma radiation had a positive effect on oleic acid, β-carotene bleaching inhibition (in infusions), DPPH scavenging activity and reducing power (in methanolic extracts). Nevertheless, it might be generally concluded that gamma radiation is less suitable than electron-beam to maintain the characteristics of dried herbs during extended storage periods.
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Exploring reserve lots of Cymbopogon citratus, Aloysia citrodora and Thymus × citriodorus as improved sources of phenolic compounds.
Food chemistry, 2018Co-Authors: Ingride Rita, Carla Pereira, Lillian Barros, Isabel C.f.r. FerreiraAbstract:Abstract Given the increasing consumers demand for novelty, tea companies have been presenting new added value products such as reserve lots of aromatic plants. Herein, infusions from different lots of three aromatic plants were assessed in terms of phenolic composition (HPLC-DAD-ESI/MS) and antioxidant properties (reducing power, free radical scavenging and lipid peroxidation inhibition capacity). Cymbopogon citratus (C. citratus; main compound 5-O-caffeoylquinic acid) and Aloysia citrodora (A. citrodora; prevalence of verbascoside) reserve lots revealed higher phenolic compounds concentration than the respective standard lots. Thymus × citriodorus (T. citriodorus; main compound rosmarinic acid) standard lot presented higher amounts of phenolic acids than the reserve lot, nonetheless, total flavonoids and phenolic compounds were not significantly different. The differences between both lots antioxidant activity were more noticeable in C. citratus, with the reserve lot presenting the highest activity. This study provides evidence of the differences between these plants chemical composition and bioactivity depending on the harvesting conditions.
Ferreira, Isabel C.f.r. - One of the best experts on this subject based on the ideXlab platform.
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Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants
2018Co-Authors: Pereira Eliana, Antonio, Amilcar L., Cabo Verde Sandra, Barros Lillian, Pimenta Andreia, Calhelha, Ricardo C., Santos-buelga Celestino, Ferreira, Isabel C.f.r.Abstract:The consumers' interest for a healthier diet and the use of natural products in the prevention and/or treatment of health problems has triggered an intensive search for bioactive plant compounds. Plants are a good source of phenolic compounds, terpenoids, alkaloids, quinones, carotenoids, sterols, glucosinolates and other compounds, which provide health benefits due to their wide range of biological properties. This work aimed to evaluate the effects of gamma radiation on the phenolic composition, cytotoxicity, virucidal and antimicrobial properties of Aloysia citrodora L. and Mentha x piperita L. infusions. Gamma radiation treatment was performed in a 60Co chamber, applying the doses of 1 and 10 kGy and the results were compared with the control sample (0 kGy). The phenolic compounds profile was obtained by HPLC-DAD-ESI/MS and the cytotoxicity was evaluated in human tumour cell lines and in a primary cell culture. For virucidal efficacy evaluation, MNV-1 and HAdV-5 viral titers were determined by plaque assay in Raw264.7 and A549 cell lines, respectively. The antibacterial activity was evaluated against Gram-negative and Gram-positive bacteria. Radiation treatment has been shown to cause different effects on phenolic concentrations and virucidal efficacy, depending on the plant species studied, the virus and the absorbed dose. In cytotoxicity assay A. citrodora did not reveal significant statistically differences considering the different doses applied, however M. piperita at 10 kGy stood out with the highest cytotoxic potential in all tested cell lines. Regarding antibacterial assays, S. aureus was the only bacteria that was sensible to the infusions of non-irradiated and 1 kGy irradiated in both species. Thus, this study contribute to a wider knowledge of the effects of gamma irradiation on several relevant bioactive potentials of some aromatic and medicinal plants and showed that gamma radiation is a technique recommended for these matrices.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and CTN (RECI/AAG-TEC/0400/2012). The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
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Effects of gamma radiation on phenolic composition of medicinal plants: Aloysia citrodora L. and Mentha X Piperita L. Infusions
2018Co-Authors: Pereira Eliana, Antonio, Amilcar L., Cabo Verde Sandra, Barros Lillian, Pimenta Andreia, Calhelha, Ricardo C., Santos-buelga Celestino, Ferreira, Isabel C.f.r.Abstract:Irradiation is a food processing technique supported by several globally recognized organizations (FAO, WHO, IAEA), being suitable for several food matrices, prolonging not only the shelf life of the food, but also providing microbiological decontamination. This work aimed to evaluate the effects of gamma radiation on the phenolic composition of Aloysia citrodora L. and Mentha x piperita L. infusions. Gamma radiation treatment was performed in a 60Co chamber, applying doses between 1 and 10 kGy and the results were compared with the control sample (non-irradiated, 0 kGy). The phenolic profile was determined and identified by HPLC-DAD-ESI/MS. Radiation treatment (at a dose of 10 kGy) caused statistically significant effects on the phenolic profile in both infusions, evidencing an increase in most of detected compounds, namely the majority ones. The same irradiation dose also induced an increase on the content of total phenolic acids and total phenolic compounds [1]. The results of this study can contribute to a wider knowledge of the effects of gamma irradiation on relevant bioactive compounds of several aromatic and medicinal plants, being recommended its application in these matrices.The authors are grateful FCT, Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and C2TN (UID/Multi/04349/2013); and to FEDER Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
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Exploring reserve lots of Cymbopogon citratus, Aloysia citrodora and Thymus x citriodorus as improved sources of phenolic compounds
'Elsevier BV', 2018Co-Authors: Rita Íngride, Barros Lillian, Pereira Carla, Ferreira, Isabel C.f.r.Abstract:Given the increasing consumers demand for novelty, tea companies have been presenting new added value products such as reserve lots of aromatic plants. Herein, infusions from different lots of three aromatic plants were assessed in terms of phenolic composition (HPLC-DAD-ESI/MS) and antioxidant properties (reducing power, free radical scavenging and lipid peroxidation inhibition capacity). Cymbopogon citratus (C. citratus; main compound 5-O-caffeoylquinic acid) and Aloysia citrodora (A. citrodora; prevalence of verbascoside) reserve lots revealed higher phenolic compounds concentration than the respective standard lots. Thymus×citriodorus (T. citriodorus; main compound rosmarinic acid) standard lot presented higher amounts of phenolic acids than the reserve lot, nonetheless, total flavonoids and phenolic compounds were not significantly different. The differences between both lots antioxidant activity were more noticeable in C. citratus, with the reserve lot presenting the highest activity. This study provides evidence of the differences between these plants chemical composition and bioactivity depending on the harvesting conditions.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013); to FCT for L. Barros research contract; to FCT for the grant attributed to C. Pereira (SFRH/BPD/122650/2016) through the Programa Operacional Capital Humano (POCH) supported by the European Social Fund and National Funds of MCTES (Ministério da Ciência, Tecnologia, e Ensino Superior). The authors are grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
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Avaliação do perfil fenólico de duas plantas comummente utilizadas na medicina tradicional, após aplicação de irradiação ionizante
2018Co-Authors: Pereira Eliana, Antonio, Amilcar L., Cabo Verde Sandra, Barros Lillian, Pimenta Andreia, Calhelha, Ricardo C., Santos-buelga Celestino, Mandim Filipa, Ferreira, Isabel C.f.r.Abstract:Os compostos fenólicos têm sido associados a diversos efeitos benéficos nomeadamente, na prevenção de algumas doenças, tais como, diabetes, cancro, doenças cardiovasculares, obesidade, doenças neuro-degenerativas, entre outras. São várias as matrizes que representam uma boa fonte deste tipo de compostos, principalmente no reino vegetal. Assim, este estudo pretendeu avaliar os efeitos da radiação gama na composição fenólica de extratos aquosos, obtidos por infusão de duas plantas comummente apreciadas pelo consumidor e usadas na medicina tradicional - Aloysia citrodora L. (limonete) e Mentha x piperita L. (hortelã-pimenta). O tratamento de irradiação foi realizado através de uma câmara de 60Co, aplicando as doses de 1 e 10 kGy e os resultados foram comparados com amostras controlo (0 kGy). O perfil fenólico foi obtido por HPLC-DAD-ESI/MS. Foram identificados 11 e 14 compostos fenólicos (derivados de flavonoides e ácidos fenólicos) nas espécies A. citrodora e M. piperita, respetivamente. Relativamente aos resultados obtidos após a aplicação deste tipo de processamento, foi evidente que as amostras submetidas a uma dose de irradiação de 10 kGy, revelaram diferenças estatisticamente significativas no perfil fenólico de ambos os extratos estudados, comparativamente com a amostra controlo (0 kGy), evidenciando um aumento na maioria dos compostos, como também nos teores de ácidos fenólicos totais e compostos fenólicos totais. Assim, este estudo contribuiu para um conhecimento mais amplo dos efeitos da irradiação gama nestas moléculas, revelando também que este tipo de processamento pode ser aplicado em plantas aromáticas e medicinais.FCT, Portugal e ao FEDER no âmbito do programa PT2020 pelo apoio financeiro ao CIMO (UID/AGR/00690/2013) e CTN (RECI/AAG-TEC/0400/2012) e contracto de L. Barros; Programa FEDER-Interreg España-Portugal pelo apoio financeiro através do projeto 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
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Effects of gamma radiation on the bioactivity of medicinal and aromatic plants: Mentha × piperita L., Thymus vulgaris L. and Aloysia citrodora Paláu as case studies
'Royal Society of Chemistry (RSC)', 2018Co-Authors: Pereira Eliana, Antonio, Amilcar L., Cabo Verde Sandra, Barros Lillian, Pimenta Andreia, Calhelha, Ricardo C., Ferreira, Isabel C.f.r.Abstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Paláu (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and the FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), C2TN (UID/Multi/04349/2013), L. Barros and R. C. Calhelha contracts. The authors are grateful to the FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
Lillian Barros - One of the best experts on this subject based on the ideXlab platform.
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Effects of gamma radiation on the bioactivity of medicinal and aromatic plants: Mentha × piperita L., Thymus vulgaris L. and Aloysia citrodora Paláu as case studies.
Food & function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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effects of gamma radiation on the bioactivity of medicinal and aromatic plants mentha piperita l thymus vulgaris l and Aloysia citrodora palau as case studies
Food & Function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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exploring reserve lots of cymbopogon citratus Aloysia citrodora and thymus citriodorus as improved sources of phenolic compounds
Food Chemistry, 2018Co-Authors: Ingride Rita, Carla Pereira, Lillian Barros, Isabel C.f.r. FerreiraAbstract:Abstract Given the increasing consumers demand for novelty, tea companies have been presenting new added value products such as reserve lots of aromatic plants. Herein, infusions from different lots of three aromatic plants were assessed in terms of phenolic composition (HPLC-DAD-ESI/MS) and antioxidant properties (reducing power, free radical scavenging and lipid peroxidation inhibition capacity). Cymbopogon citratus (C. citratus; main compound 5-O-caffeoylquinic acid) and Aloysia citrodora (A. citrodora; prevalence of verbascoside) reserve lots revealed higher phenolic compounds concentration than the respective standard lots. Thymus × citriodorus (T. citriodorus; main compound rosmarinic acid) standard lot presented higher amounts of phenolic acids than the reserve lot, nonetheless, total flavonoids and phenolic compounds were not significantly different. The differences between both lots antioxidant activity were more noticeable in C. citratus, with the reserve lot presenting the highest activity. This study provides evidence of the differences between these plants chemical composition and bioactivity depending on the harvesting conditions.
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Evaluation of gamma-irradiated aromatic herbs: Chemometric study of samples submitted to extended storage periods.
Food research international (Ottawa Ont.), 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, João C.m. Barreira, M. Beatriz P.p. Oliveira, Isabel C.f.r. FerreiraAbstract:Abstract The preserving capacity of gamma radiation (10 kGy) on the chemical, nutritional and antioxidant components of Aloysia citrodora Palau, Melissa officinalis L., Melittis melissophyllum L. and Mentha piperita L., stored for 12 and 18 months, was evaluated. Despite the maintenance of the main characteristics during the first 12 months of storage, the additional 6 months induced several significant changes in individual compounds. In general, the analyzed species reacted dissimilarly throughout time, but it was possible to verify that the fatty acids, tocopherols and antioxidant capacity presented the most significant changes after 18 months of storage, inclusively in samples submitted to gamma radiation. In fact, the applied treatment (10 kGy) did not seem to be effective to prevent the decrease of free sugars, organic acids and tocopherols, especially considering the 18 months period. On the other hand, the evolution in color parameters indicated a greener color (yet slightly more yellow) among irradiated samples. Likewise, gamma radiation had a positive effect on oleic acid, β-carotene bleaching inhibition (in infusions), DPPH scavenging activity and reducing power (in methanolic extracts). Nevertheless, it might be generally concluded that gamma radiation is less suitable than electron-beam to maintain the characteristics of dried herbs during extended storage periods.
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Exploring reserve lots of Cymbopogon citratus, Aloysia citrodora and Thymus × citriodorus as improved sources of phenolic compounds.
Food chemistry, 2018Co-Authors: Ingride Rita, Carla Pereira, Lillian Barros, Isabel C.f.r. FerreiraAbstract:Abstract Given the increasing consumers demand for novelty, tea companies have been presenting new added value products such as reserve lots of aromatic plants. Herein, infusions from different lots of three aromatic plants were assessed in terms of phenolic composition (HPLC-DAD-ESI/MS) and antioxidant properties (reducing power, free radical scavenging and lipid peroxidation inhibition capacity). Cymbopogon citratus (C. citratus; main compound 5-O-caffeoylquinic acid) and Aloysia citrodora (A. citrodora; prevalence of verbascoside) reserve lots revealed higher phenolic compounds concentration than the respective standard lots. Thymus × citriodorus (T. citriodorus; main compound rosmarinic acid) standard lot presented higher amounts of phenolic acids than the reserve lot, nonetheless, total flavonoids and phenolic compounds were not significantly different. The differences between both lots antioxidant activity were more noticeable in C. citratus, with the reserve lot presenting the highest activity. This study provides evidence of the differences between these plants chemical composition and bioactivity depending on the harvesting conditions.
Sandra Cabo Verde - One of the best experts on this subject based on the ideXlab platform.
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Target specific post-harvest treatment by gamma radiation for the microbial safety of dried Melissa officinalis and Aloysia citrodora.
Applied radiation and isotopes : including data instrumentation and methods for use in agriculture industry and medicine, 2020Co-Authors: Márcia Meneses, Amilcar L. Antonio, Sandra Cabo VerdeAbstract:This study aimed to assess a specific gamma radiation dose to be applied as a post-harvest process to guarantee the microbial safety of two medicinal plants, Melissa officinalis and Aloysia citrodora. Dried plants treated with gamma radiation indicated that a dose of 5 kGy could be applied as a post-harvest treatment process of M. officinalis and A. citrodora, assuring the microbial safety of dried medicinal plants and lowering the potentiality of deleterious effects on plants' quality attributes. This will enhance the safety and quality of the dried plants to be used as raw materials in industrial applications.
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Effects of gamma radiation on the bioactivity of medicinal and aromatic plants: Mentha × piperita L., Thymus vulgaris L. and Aloysia citrodora Paláu as case studies.
Food & function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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effects of gamma radiation on the bioactivity of medicinal and aromatic plants mentha piperita l thymus vulgaris l and Aloysia citrodora palau as case studies
Food & Function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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Evaluation of gamma-irradiated aromatic herbs: Chemometric study of samples submitted to extended storage periods.
Food research international (Ottawa Ont.), 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, João C.m. Barreira, M. Beatriz P.p. Oliveira, Isabel C.f.r. FerreiraAbstract:Abstract The preserving capacity of gamma radiation (10 kGy) on the chemical, nutritional and antioxidant components of Aloysia citrodora Palau, Melissa officinalis L., Melittis melissophyllum L. and Mentha piperita L., stored for 12 and 18 months, was evaluated. Despite the maintenance of the main characteristics during the first 12 months of storage, the additional 6 months induced several significant changes in individual compounds. In general, the analyzed species reacted dissimilarly throughout time, but it was possible to verify that the fatty acids, tocopherols and antioxidant capacity presented the most significant changes after 18 months of storage, inclusively in samples submitted to gamma radiation. In fact, the applied treatment (10 kGy) did not seem to be effective to prevent the decrease of free sugars, organic acids and tocopherols, especially considering the 18 months period. On the other hand, the evolution in color parameters indicated a greener color (yet slightly more yellow) among irradiated samples. Likewise, gamma radiation had a positive effect on oleic acid, β-carotene bleaching inhibition (in infusions), DPPH scavenging activity and reducing power (in methanolic extracts). Nevertheless, it might be generally concluded that gamma radiation is less suitable than electron-beam to maintain the characteristics of dried herbs during extended storage periods.
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infusions of gamma irradiated Aloysia citrodora l and mentha x piperita l effects on phenolic composition cytotoxicity antibacterial and virucidal activities
Industrial Crops and Products, 2017Co-Authors: Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Eliana Pereira, Andreia I. Pimenta, Ricardo C. Calhelha, Celestino Santosbuelga, Isabel C.f.r. FerreiraAbstract:Abstract The incorporation of plants in industry requires high standards of hygiene, being the raw materials obligatorily decontaminated. Irradiation is increasingly applied as a decontamination technique in several countries. This work aimed to evaluate the effects of gamma radiation on the phenolic composition, cytotoxicity, virucidal and antimicrobial properties of Aloysia citrodora L. (lemon verbena) and Mentha x piperita L. (peppermint) infusions. Phenolic compounds profile was obtained by HPLC-DAD-ESI/MS and cytotoxicity was evaluated in human tumour and non-tumour cell lines; in virucidal efficacy, MNV-1 and HAdV-5 viral titers were determined by plaque assay in Raw264.7 and A549 cell lines, respectively; and the antibacterial potential was evaluated against Gram-negative and Gram-positive bacteria. Radiation treatment caused a significant increase in total phenolic compounds of both plants, while the virucidal efficacy was depend on the plant, the virus, and absorbed dose. S. aureus , with MIC and MBC values of 5 mg/mL, was the only bacteria sensible to the infusions of non-irradiated and 1 kGy irradiated lemon verbena and peppermint samples; gamma radiation appears to cause no alteration in the antibacterial potential of the studied plants. Peppermint infusion (irradiated at 10 kGy) showed the highest cytotoxic potential in all tumour cell lines.
Eliana Pereira - One of the best experts on this subject based on the ideXlab platform.
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effects of gamma radiation on the bioactivity of medicinal and aromatic plants mentha piperita l thymus vulgaris l and Aloysia citrodora palau as case studies
Food & Function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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Effects of gamma radiation on the bioactivity of medicinal and aromatic plants: Mentha × piperita L., Thymus vulgaris L. and Aloysia citrodora Paláu as case studies.
Food & function, 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Andreia I. Pimenta, Ricardo C. Calhelha, Isabel C.f.r. FerreiraAbstract:Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial activities) of Aloysia citrodora Palau (lemon verbena), Mentha × piperita L. (peppermint) and Thymus vulgaris L. (thyme). The observed cytotoxic activity varied with the plant and with the applied dose, being higher in Thymus vulgaris irradiated with 10 kGy. The virucidal activity was also dependent on the radiation dose, but was preserved with irradiation treatment. Gamma rays had no effect on the antimicrobial activity of the studied plants. Otherwise, the effects of gamma radiation on the phenolic profile were heterogeneous, with an increase in some compounds and decrease in others, depending on the species and on the radiation dose.
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Evaluation of gamma-irradiated aromatic herbs: Chemometric study of samples submitted to extended storage periods.
Food research international (Ottawa Ont.), 2018Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, João C.m. Barreira, M. Beatriz P.p. Oliveira, Isabel C.f.r. FerreiraAbstract:Abstract The preserving capacity of gamma radiation (10 kGy) on the chemical, nutritional and antioxidant components of Aloysia citrodora Palau, Melissa officinalis L., Melittis melissophyllum L. and Mentha piperita L., stored for 12 and 18 months, was evaluated. Despite the maintenance of the main characteristics during the first 12 months of storage, the additional 6 months induced several significant changes in individual compounds. In general, the analyzed species reacted dissimilarly throughout time, but it was possible to verify that the fatty acids, tocopherols and antioxidant capacity presented the most significant changes after 18 months of storage, inclusively in samples submitted to gamma radiation. In fact, the applied treatment (10 kGy) did not seem to be effective to prevent the decrease of free sugars, organic acids and tocopherols, especially considering the 18 months period. On the other hand, the evolution in color parameters indicated a greener color (yet slightly more yellow) among irradiated samples. Likewise, gamma radiation had a positive effect on oleic acid, β-carotene bleaching inhibition (in infusions), DPPH scavenging activity and reducing power (in methanolic extracts). Nevertheless, it might be generally concluded that gamma radiation is less suitable than electron-beam to maintain the characteristics of dried herbs during extended storage periods.
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infusions of gamma irradiated Aloysia citrodora l and mentha x piperita l effects on phenolic composition cytotoxicity antibacterial and virucidal activities
Industrial Crops and Products, 2017Co-Authors: Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Eliana Pereira, Andreia I. Pimenta, Ricardo C. Calhelha, Celestino Santosbuelga, Isabel C.f.r. FerreiraAbstract:Abstract The incorporation of plants in industry requires high standards of hygiene, being the raw materials obligatorily decontaminated. Irradiation is increasingly applied as a decontamination technique in several countries. This work aimed to evaluate the effects of gamma radiation on the phenolic composition, cytotoxicity, virucidal and antimicrobial properties of Aloysia citrodora L. (lemon verbena) and Mentha x piperita L. (peppermint) infusions. Phenolic compounds profile was obtained by HPLC-DAD-ESI/MS and cytotoxicity was evaluated in human tumour and non-tumour cell lines; in virucidal efficacy, MNV-1 and HAdV-5 viral titers were determined by plaque assay in Raw264.7 and A549 cell lines, respectively; and the antibacterial potential was evaluated against Gram-negative and Gram-positive bacteria. Radiation treatment caused a significant increase in total phenolic compounds of both plants, while the virucidal efficacy was depend on the plant, the virus, and absorbed dose. S. aureus , with MIC and MBC values of 5 mg/mL, was the only bacteria sensible to the infusions of non-irradiated and 1 kGy irradiated lemon verbena and peppermint samples; gamma radiation appears to cause no alteration in the antibacterial potential of the studied plants. Peppermint infusion (irradiated at 10 kGy) showed the highest cytotoxic potential in all tumour cell lines.
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Is Gamma Radiation Suitable to Preserve Phenolic Compounds and to Decontaminate Mycotoxins in Aromatic Plants? A Case-Study with Aloysia citrodora Paláu
Molecules (Basel Switzerland), 2017Co-Authors: Eliana Pereira, Amilcar L. Antonio, Sandra Cabo Verde, Lillian Barros, Isabel C.f.r. Ferreira, Celestino Santos-buelga, Paula RodriguesAbstract:This study aimed to determine the effect of gamma radiation on the preservation of phenolic compounds and on decontamination of dry herbs in terms of ochratoxin A (OTA) and aflatoxin B1 (AFB1), using Aloysia citrodora Palau as a case study. For this purpose, artificially contaminated dry leaves were submitted to gamma radiation at different doses (1, 5, and 10 kGy; at dose rate of 1.7 kGy/h). Phenolic compounds were analysed by HPLC-DAD-ESI/MS and mycotoxin levels were determined by HPLC-fluorescence. Eleven phenolic compounds were identified in the samples and despite the apparent degradation of some compounds (namely verbasoside), 1 and 10 kGy doses point to a preservation of the majority of the compounds. The mean mycotoxin reduction varied between 5.3% and 9.6% for OTA and from 4.9% to 5.2% for AFB1. It was not observed a significant effect of the irradiation treatments on mycotoxin levels, and a slight degradation of the phenolic compounds in the irradiated samples was observed.
