The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
Shengjun Mao - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of Alpha Asarone on the rats model of cerebral ischemia reperfusion stroke via ameliorating glial activation and autophagy
Neuroscience, 2021Co-Authors: Kun Zhang, Qi Liu, Lijun Luo, Xinqian Feng, Xiangyu Fan, Shengjun MaoAbstract:Abstract Alpha-Asarone, a major active component isolated from Acorus gramineus, can affect brain functions and behaviors by multiple mechanisms. However, the effect of Alpha-Asarone on cerebral ischemia–reperfusion (CIR) stroke has not been reported. The present study aimed to investigate the neuroprotective effect of Alpha-Asarone and the involved mechanisms against CIR stroke. Rats were subjected to middle cerebral occlusion (MCAO) for 2 h. Then the drug or drug-free vehicle was intravenously injected to corresponding groups. After reperfusion for 24 h, the infarct volume was evaluated by Triphenyl Tetrazolium Chloride (TTC) staining. The neurofunctional recovery and post-stroke epilepsy were evaluated. Nissl and Hematoxylin–Eosin (H&E) staining were used for histological observation. We investigated the protective mechanism of Alpha-Asarone against the stroke. The results showed that Alpha-Asarone exhibited a desirable neuroprotective effect, manifested as reducing infarct volume and post-stroke epilepsy and improving neurological function. Histological and flow cytometry analysis revealed that Alpha-Asarone treatment alleviated cell injury and apoptosis in vivo and in vitro. Furthermore, Alpha-Asarone decreased GFAP, Iba-1, and LC3II/LC3I expression and increased the expression of p62. These results suggested that Alpha-Asarone attenuated the CIR stroke injury via ameliorating glial activation and autophagy.
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Alpha Asarone improves cognitive function of app ps1 mice and reducing aβ42 p tau and neuroinflammation and promoting neuron survival in the hippocampus
Neuroscience, 2021Co-Authors: Lili Zeng, Kun Zhang, Qi Liu, Di Zhang, Jian Zhang, Xiaofeng Gao, Qiantao Wang, Yongxiang Zheng, Shengjun MaoAbstract:Alzheimer's disease (AD) is a progressive neurodegenerative disease most often characterized by memory impairment and cognitive decline. Alpha-Asarone has been reported to have the potential to treat AD. Our previous studies have found that Alpha-Asarone improves aged rats' cognitive function by alleviating neuronal excitotoxicity via type A gamma-aminobutyric acid (GABA) receptors. GABA level's change, neuroinflammation, and dysfunctional autophagy are found to be associated with AD. However, the effect of Alpha-Asarone on cognitive function of APP/PS1 transgenic mice and its underlying mechanism in terms of aggregation of amyloid-β42 (Aβ42) and phosphorylated tau (p-tau), glutamic acid decarboxylase (GAD) level, neuroinflammation, and autophagy are unclear. Accordingly, we attempted to explore whether Alpha-Asarone improves AD mice's cognitive function and alleviates pathological symptoms by regulating GAD level, inhibiting neuroinflammation, or restore autophagy. We found that Alpha-Asarone enhanced spatial learning memory and decreased Aβ42 and p-tau levels without influencing the GAD level in APP/PS1 transgenic mice. Also, it decreased the GFAP expression and reduced pro-inflammatory cytokines levels, thus alleviating neuroinflammation. Furthermore, Alpha-Asarone decreased the excess number of autophagosomes and promoted hippocampal neurons' survival. In conclusion, the results confirmed the therapeutic effect of Alpha-Asarone on AD-related astrogliosis, dysfunctional autophagy, and neuronal damage, which indicates its great potential to treat AD.
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Alpha Asarone improves cognitive function of aged rats by alleviating neuronal excitotoxicity via gabaa receptors
Neuropharmacology, 2020Co-Authors: Yu Chen, Kun Zhang, Qi Liu, Lili Zeng, Di Zhang, Xiaofeng Gao, Qiantao Wang, Huixi Zou, Shengjun MaoAbstract:Alzheimer's disease (AD), the most common form of dementia, still lacks effective treatment at present. Alpha-Asarone (ASA) is the major compound isolated from the Chinese medicinal herb Acorus gramineus. It has been reported to enhance cognitive function in rodent models, yet its mechanism was not fully understood. In this work, we demonstrated that ASA improved the spatial memory, reduced the neuronal injury, and decreased the level of Aβ1-42 in the hippocampus of aged rats. The results also showed that ASA had the neuroprotective effects against glutamate toxicity and decreased cytoplasmic calcium level in primary hippocampal neurons. By comparing the multiple properties of ASA and propofol (PPF) via computer modelling, we speculated that ASA may bind to the PPF binding site of type A gamma (γ)-aminobutyric acid receptors (GABAARs). This was further supported by the whole-cell patch-clamp recording. Our results suggested that ASA, as a GABAAR positive allosteric modulator (PAM), can improve cognitive function of aged rats by alleviating the neuronal overexcitation. Furthermore, the binding mode of ASA on GABAAR may lay a foundation for structure-based drug design in AD therapy.
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comparison of kinetic behavior in both plasma and tissue after intravenous administration of Alpha Asarone in lipid emulsion and aqueous solution in rats and mice
China journal of Chinese materia medica, 2008Co-Authors: Dandan Guo, Shengjun Mao, Shixiang Hou, Rongli ZhaoAbstract:OBJECTIVE To compare the pharmacokinetics and tissue distribution of Alpha-Asarone in lipid emulsion and aqueous solution for injection and study the feasibility of lipid emulsion of Alpha-Asarone as the parenteral drug delivery system. METHOD HPLC was used to determine the drug concentration in rat plasma and mice tissues after intravenous (i.v.) administration of lipid emulsion and aqueous solution of Alpha-Asarone at a single dose (40 mg x kg(-1)), respectively. RESULT The plasma concentration-time profiles of lipid emulsion and aqueous solution of Alpha-Asarone after intravenous administration of them are similar and the drug concentration-time data were fitted to a two-compartment open model. The results of tissues distribution showed that distribution contents of Alpha-Asarone from lipid emulsion and aqueous solution in vivo are similar in lungs but lipid emulsion increased the uptake in livers and spleens, and decreased the uptake in hearts and kidneys for Alpha-Asarone. CONCLUSION The plasma concentration-time profiles of Alpha-Asarone in lipid emulsion and aqueous solution are similar, but lipid emulsion significantly altered the tissue distribution of Alpha-Asarone, which may be beneficial to decrease its potential toxicity to heart and kidney.
Kun Zhang - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of Alpha Asarone on the rats model of cerebral ischemia reperfusion stroke via ameliorating glial activation and autophagy
Neuroscience, 2021Co-Authors: Kun Zhang, Qi Liu, Lijun Luo, Xinqian Feng, Xiangyu Fan, Shengjun MaoAbstract:Abstract Alpha-Asarone, a major active component isolated from Acorus gramineus, can affect brain functions and behaviors by multiple mechanisms. However, the effect of Alpha-Asarone on cerebral ischemia–reperfusion (CIR) stroke has not been reported. The present study aimed to investigate the neuroprotective effect of Alpha-Asarone and the involved mechanisms against CIR stroke. Rats were subjected to middle cerebral occlusion (MCAO) for 2 h. Then the drug or drug-free vehicle was intravenously injected to corresponding groups. After reperfusion for 24 h, the infarct volume was evaluated by Triphenyl Tetrazolium Chloride (TTC) staining. The neurofunctional recovery and post-stroke epilepsy were evaluated. Nissl and Hematoxylin–Eosin (H&E) staining were used for histological observation. We investigated the protective mechanism of Alpha-Asarone against the stroke. The results showed that Alpha-Asarone exhibited a desirable neuroprotective effect, manifested as reducing infarct volume and post-stroke epilepsy and improving neurological function. Histological and flow cytometry analysis revealed that Alpha-Asarone treatment alleviated cell injury and apoptosis in vivo and in vitro. Furthermore, Alpha-Asarone decreased GFAP, Iba-1, and LC3II/LC3I expression and increased the expression of p62. These results suggested that Alpha-Asarone attenuated the CIR stroke injury via ameliorating glial activation and autophagy.
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Alpha Asarone improves cognitive function of app ps1 mice and reducing aβ42 p tau and neuroinflammation and promoting neuron survival in the hippocampus
Neuroscience, 2021Co-Authors: Lili Zeng, Kun Zhang, Qi Liu, Di Zhang, Jian Zhang, Xiaofeng Gao, Qiantao Wang, Yongxiang Zheng, Shengjun MaoAbstract:Alzheimer's disease (AD) is a progressive neurodegenerative disease most often characterized by memory impairment and cognitive decline. Alpha-Asarone has been reported to have the potential to treat AD. Our previous studies have found that Alpha-Asarone improves aged rats' cognitive function by alleviating neuronal excitotoxicity via type A gamma-aminobutyric acid (GABA) receptors. GABA level's change, neuroinflammation, and dysfunctional autophagy are found to be associated with AD. However, the effect of Alpha-Asarone on cognitive function of APP/PS1 transgenic mice and its underlying mechanism in terms of aggregation of amyloid-β42 (Aβ42) and phosphorylated tau (p-tau), glutamic acid decarboxylase (GAD) level, neuroinflammation, and autophagy are unclear. Accordingly, we attempted to explore whether Alpha-Asarone improves AD mice's cognitive function and alleviates pathological symptoms by regulating GAD level, inhibiting neuroinflammation, or restore autophagy. We found that Alpha-Asarone enhanced spatial learning memory and decreased Aβ42 and p-tau levels without influencing the GAD level in APP/PS1 transgenic mice. Also, it decreased the GFAP expression and reduced pro-inflammatory cytokines levels, thus alleviating neuroinflammation. Furthermore, Alpha-Asarone decreased the excess number of autophagosomes and promoted hippocampal neurons' survival. In conclusion, the results confirmed the therapeutic effect of Alpha-Asarone on AD-related astrogliosis, dysfunctional autophagy, and neuronal damage, which indicates its great potential to treat AD.
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Alpha Asarone improves cognitive function of aged rats by alleviating neuronal excitotoxicity via gabaa receptors
Neuropharmacology, 2020Co-Authors: Yu Chen, Kun Zhang, Qi Liu, Lili Zeng, Di Zhang, Xiaofeng Gao, Qiantao Wang, Huixi Zou, Shengjun MaoAbstract:Alzheimer's disease (AD), the most common form of dementia, still lacks effective treatment at present. Alpha-Asarone (ASA) is the major compound isolated from the Chinese medicinal herb Acorus gramineus. It has been reported to enhance cognitive function in rodent models, yet its mechanism was not fully understood. In this work, we demonstrated that ASA improved the spatial memory, reduced the neuronal injury, and decreased the level of Aβ1-42 in the hippocampus of aged rats. The results also showed that ASA had the neuroprotective effects against glutamate toxicity and decreased cytoplasmic calcium level in primary hippocampal neurons. By comparing the multiple properties of ASA and propofol (PPF) via computer modelling, we speculated that ASA may bind to the PPF binding site of type A gamma (γ)-aminobutyric acid receptors (GABAARs). This was further supported by the whole-cell patch-clamp recording. Our results suggested that ASA, as a GABAAR positive allosteric modulator (PAM), can improve cognitive function of aged rats by alleviating the neuronal overexcitation. Furthermore, the binding mode of ASA on GABAAR may lay a foundation for structure-based drug design in AD therapy.
Germán Chamorro - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of Alpha Asarone on spatial memory and nitric oxide levels in rats injected with amyloid β 25 35
Neuroscience Letters, 2009Co-Authors: Ilhuicamina Daniel Limon, Germán Chamorro, Liliana Mendieta, Alfonso Diaz, Blanca Espinosa, Edgar Zenteno, Jorge GuevaraAbstract:Abstract The chemical α-Asarone is an important active substance of the Acori graminei rhizome (AGR). It has pharmacological effects that include antihyperlipidemic, antiinflammatory, and antioxidant activity. Our aim was to study the effects α-Asarone on nitric oxide (NO) levels in the hippocampus and temporal cortex of the rat after injection of the fraction 25–35 from amyloid-β (Aβ(25–35)). In addition we examined the working spatial memory in an eight-arm radial maze. Our results showed a significant increase of nitrites in the hippocampus and temporal cortex of Aβ(25–35)-treated rats. Other evidence of neuronal damage was the expression of a glial-fibrillar-acid protein and a silver staining. There were impairments in the spatial memory evaluated in the eight-arm radial maze. We wanted to determine whether α-Asarone improves the memory correlated with NO overproduction and neuronal damage caused by the injection of Aβ(25–35) into rats. Then animals received a 16-day treatment of α-Asarone before the Aβ(25–35) injection. Our results show a significant decrease of nitrite levels in the hippocampus and temporal cortex, without astrocytosis and silver-staining cells, which correlates with memory improvement in the α-Asarone-treated group. Our results suggest that α-Asarone may protect neurons against Aβ(25–35)–caused neurotoxicity by inhibiting the effects of NO overproduction in the hippocampus and temporal cortex.
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effect of α Asarone and a derivative on lipids bile flow and na k atpase in ethinyl estradiol induced cholestasis in the rat
Fundamental & Clinical Pharmacology, 2007Co-Authors: Leticia Gardunosiciliano, Fernando Labarrios, Joaquín Tamariz, Germán Chamorro, Mario G Moreno, Pablo MurielAbstract:Administration of ethinyl estradiol (EE), a widely used component of oral contraceptives, has been associated with impairment of bile flow and the capacity to excrete organic anions in man and experimental animals. Alpha-Asarone (2,4,5-trimethoxypropenylbenzene) and 2-methoxy-4-(2-propenyl) phenoxyacetic acid (MPPA) have shown hypolipidemic effects. In addition to these effects, we decided to evaluate the properties of these compounds on EE-induced cholestasis. Wistar male rats were injected subcutaneously with 10 mg/kg of EE for 5 days; simultaneously, Alpha-Asarone or MPPA were also administered and appropriate controls were performed. Alpha-Asarone and MPPA decreased plasma and bile cholesterol. EE diminished triglycerides total, low-density lipoprotein, high-density lipoprotein and bile cholesterol. MPPA further decreased these lipid parameters. Alkaline phosphatase (an enzyme marker of cholestasis) was increased after administration of EE, but this effect was prevented significantly by Alpha-Asarone or MPPA administration. Bile flow was importantly decreased by EE and increased by Alpha-Asarone alone. Furthermore, Alpha-Asarone or MPPA preserved the normal bile flow in EE-treated rats. EE inhibited the activity of the Na(+)/K(+)-ATPase, while both Alpha-Asarone and MPPA preserved this enzyme activity. Na(+)/K(+)-ATPase is involved in Na(+)-coupled uptake of bile acids into hepatocytes and, therefore, ultimately is the driving force for the generation of bile flow. Therefore, the anticholestatic effects of Alpha-Asarone and MPPA, described herein by the first time, may be due to its ability to preserve ATPase activity. This enzyme is negatively regulated by membrane cholesterol, thus the hypolipidemic effects of the compounds tested may be responsible for Na(+)/K(+)-ATPase activity and bile flow maintenance.
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hypolipidaemic and antiplatelet activity of phenoxyacetic acid derivatives related to α Asarone
Journal of Pharmacy and Pharmacology, 2006Co-Authors: Ricardo Perezpasten, Rosa Virginia García, Leticia Garduño, Elba Reyes, Fernando Labarrios, Joaquín Tamariz, Germán ChamorroAbstract:The phenoxyacetic acid derivatives 1-6 [2-methoxy-4-(2-propenyl)phenoxyacetic acid (1); 2-methoxy-5-nitro-4-(2-propenyl)phenoxyacetic acid (2); methyl 2-methoxy-4-(2-propenyl)phenoxyacetate (3); ethyl 2-methoxy-4-(2-propenyl)phenoxyacetate (4); methyl 2-methoxy-5-nitro-4-(2-propenyl)phenoxyacetate (5); ethyl 2-methoxy-5-nitro-4-(2-propenyl)phenoxyacetate (6)] related to Alpha-Asarone have been reported previously as hypolipidaemic agents in diet-induced hyperlipidaemic mice. We have aimed to expand the pharmacological profile of these derivatives by investigating their hypolipidaemic activity in rats and mice under different experimental conditions. The antiplatelet activity was tested also in-vitro from blood derived from consenting healthy volunteers. In normolipidaemic rats, compounds 2, 3 and 5 at oral doses of 40 and 80 mg kg(-1) significantly decreased total cholesterol and LDL-cholesterol levels. Moreover, analogues 3 and 5 administered to hypercholesterolaemic rats at the same doses for seven days also produced a reduction in the content of these same lipoproteins. In neither case were the high-density lipoprotein cholesterol and triglyceride concentrations affected. However, practically all tested compounds were found to be hypocholesterolaemic agents, and were shown to effectively lower low-density lipoprotein cholesterol and triglyceride levels in Triton-induced hyperlipidaemic mice at oral doses of 50 and 100 mg kg(-1). In all tests, all animals appeared to be healthy throughout the experimental period in their therapeutic ranges. Triton-induced hypercholesterolaemic mice appeared to be a desirable model for this class of hypolipidaemic drugs. On the other hand, compounds 1, 2, 4 and 5 significantly inhibited ADP-induced aggregation in-vitro. These findings indicated that all of these compounds appeared to be promising for the treatment of human hyperlipidaemia and thrombotic diseases.
Jorge Guevara - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of Alpha Asarone on spatial memory and nitric oxide levels in rats injected with amyloid β 25 35
Neuroscience Letters, 2009Co-Authors: Ilhuicamina Daniel Limon, Germán Chamorro, Liliana Mendieta, Alfonso Diaz, Blanca Espinosa, Edgar Zenteno, Jorge GuevaraAbstract:Abstract The chemical α-Asarone is an important active substance of the Acori graminei rhizome (AGR). It has pharmacological effects that include antihyperlipidemic, antiinflammatory, and antioxidant activity. Our aim was to study the effects α-Asarone on nitric oxide (NO) levels in the hippocampus and temporal cortex of the rat after injection of the fraction 25–35 from amyloid-β (Aβ(25–35)). In addition we examined the working spatial memory in an eight-arm radial maze. Our results showed a significant increase of nitrites in the hippocampus and temporal cortex of Aβ(25–35)-treated rats. Other evidence of neuronal damage was the expression of a glial-fibrillar-acid protein and a silver staining. There were impairments in the spatial memory evaluated in the eight-arm radial maze. We wanted to determine whether α-Asarone improves the memory correlated with NO overproduction and neuronal damage caused by the injection of Aβ(25–35) into rats. Then animals received a 16-day treatment of α-Asarone before the Aβ(25–35) injection. Our results show a significant decrease of nitrite levels in the hippocampus and temporal cortex, without astrocytosis and silver-staining cells, which correlates with memory improvement in the α-Asarone-treated group. Our results suggest that α-Asarone may protect neurons against Aβ(25–35)–caused neurotoxicity by inhibiting the effects of NO overproduction in the hippocampus and temporal cortex.
Qi Liu - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of Alpha Asarone on the rats model of cerebral ischemia reperfusion stroke via ameliorating glial activation and autophagy
Neuroscience, 2021Co-Authors: Kun Zhang, Qi Liu, Lijun Luo, Xinqian Feng, Xiangyu Fan, Shengjun MaoAbstract:Abstract Alpha-Asarone, a major active component isolated from Acorus gramineus, can affect brain functions and behaviors by multiple mechanisms. However, the effect of Alpha-Asarone on cerebral ischemia–reperfusion (CIR) stroke has not been reported. The present study aimed to investigate the neuroprotective effect of Alpha-Asarone and the involved mechanisms against CIR stroke. Rats were subjected to middle cerebral occlusion (MCAO) for 2 h. Then the drug or drug-free vehicle was intravenously injected to corresponding groups. After reperfusion for 24 h, the infarct volume was evaluated by Triphenyl Tetrazolium Chloride (TTC) staining. The neurofunctional recovery and post-stroke epilepsy were evaluated. Nissl and Hematoxylin–Eosin (H&E) staining were used for histological observation. We investigated the protective mechanism of Alpha-Asarone against the stroke. The results showed that Alpha-Asarone exhibited a desirable neuroprotective effect, manifested as reducing infarct volume and post-stroke epilepsy and improving neurological function. Histological and flow cytometry analysis revealed that Alpha-Asarone treatment alleviated cell injury and apoptosis in vivo and in vitro. Furthermore, Alpha-Asarone decreased GFAP, Iba-1, and LC3II/LC3I expression and increased the expression of p62. These results suggested that Alpha-Asarone attenuated the CIR stroke injury via ameliorating glial activation and autophagy.
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Alpha Asarone improves cognitive function of app ps1 mice and reducing aβ42 p tau and neuroinflammation and promoting neuron survival in the hippocampus
Neuroscience, 2021Co-Authors: Lili Zeng, Kun Zhang, Qi Liu, Di Zhang, Jian Zhang, Xiaofeng Gao, Qiantao Wang, Yongxiang Zheng, Shengjun MaoAbstract:Alzheimer's disease (AD) is a progressive neurodegenerative disease most often characterized by memory impairment and cognitive decline. Alpha-Asarone has been reported to have the potential to treat AD. Our previous studies have found that Alpha-Asarone improves aged rats' cognitive function by alleviating neuronal excitotoxicity via type A gamma-aminobutyric acid (GABA) receptors. GABA level's change, neuroinflammation, and dysfunctional autophagy are found to be associated with AD. However, the effect of Alpha-Asarone on cognitive function of APP/PS1 transgenic mice and its underlying mechanism in terms of aggregation of amyloid-β42 (Aβ42) and phosphorylated tau (p-tau), glutamic acid decarboxylase (GAD) level, neuroinflammation, and autophagy are unclear. Accordingly, we attempted to explore whether Alpha-Asarone improves AD mice's cognitive function and alleviates pathological symptoms by regulating GAD level, inhibiting neuroinflammation, or restore autophagy. We found that Alpha-Asarone enhanced spatial learning memory and decreased Aβ42 and p-tau levels without influencing the GAD level in APP/PS1 transgenic mice. Also, it decreased the GFAP expression and reduced pro-inflammatory cytokines levels, thus alleviating neuroinflammation. Furthermore, Alpha-Asarone decreased the excess number of autophagosomes and promoted hippocampal neurons' survival. In conclusion, the results confirmed the therapeutic effect of Alpha-Asarone on AD-related astrogliosis, dysfunctional autophagy, and neuronal damage, which indicates its great potential to treat AD.
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Alpha Asarone improves cognitive function of aged rats by alleviating neuronal excitotoxicity via gabaa receptors
Neuropharmacology, 2020Co-Authors: Yu Chen, Kun Zhang, Qi Liu, Lili Zeng, Di Zhang, Xiaofeng Gao, Qiantao Wang, Huixi Zou, Shengjun MaoAbstract:Alzheimer's disease (AD), the most common form of dementia, still lacks effective treatment at present. Alpha-Asarone (ASA) is the major compound isolated from the Chinese medicinal herb Acorus gramineus. It has been reported to enhance cognitive function in rodent models, yet its mechanism was not fully understood. In this work, we demonstrated that ASA improved the spatial memory, reduced the neuronal injury, and decreased the level of Aβ1-42 in the hippocampus of aged rats. The results also showed that ASA had the neuroprotective effects against glutamate toxicity and decreased cytoplasmic calcium level in primary hippocampal neurons. By comparing the multiple properties of ASA and propofol (PPF) via computer modelling, we speculated that ASA may bind to the PPF binding site of type A gamma (γ)-aminobutyric acid receptors (GABAARs). This was further supported by the whole-cell patch-clamp recording. Our results suggested that ASA, as a GABAAR positive allosteric modulator (PAM), can improve cognitive function of aged rats by alleviating the neuronal overexcitation. Furthermore, the binding mode of ASA on GABAAR may lay a foundation for structure-based drug design in AD therapy.