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Alpha-Tocopherol Transfer Protein

The Experts below are selected from a list of 222 Experts worldwide ranked by ideXlab platform

Maret G Traber – 1st expert on this subject based on the ideXlab platform

  • Vitamin E is Necessary to Protect Neural Crest Cells in Developing Zebrafish Embryos
    Current Developments in Nutrition, 2020
    Co-Authors: Brian Head, Robyn L Tanguay, Chrissa Kioussi, Maret G Traber

    Abstract:

    Abstract

    Objectives
    Vitamin E (VitE) deficiency causes vertebrate embryonic lethality. The Alpha-Tocopherol Transfer Protein (Ttpa) likely regulates VitE distribution in the early zebrafish embryo because Ttpa knockdown causes impaired nervous system development and embryonic death by 15–18 hours post-fertilization (hpf). We propose that VitE is necessary for normal brain and peripheral nervous system development.

    Methods
    Zebrafish embryos are obtained from adults fed either VitE sufficient (E+) or deficient (E–) diets for at least 80 days. Embryos at 12 and 24 hpf are subjected to RNA whole mount in situ hybridization (WISH). RNA is also collected from embryos at 12, 18 and 24 hpf for RT-qPCR of specific targets.

    Results
    At 12 hpf, the midbrain-hindbrain boundary and otic placodes are malformed in E– embryos, as shown by Pax2a expression. Similarly, Sox10 expression shows that E– embryos lack clear neural plate borders. Nonetheless, in 12 hpf E + and E− embryos Ttpa is localized similarly throughout the nervous system. Pax2a expression initiates collagen formation in the developing notochord. Collagen genes, col2a1a and col9a2, expression patterns showed abnormal notochord structures in 24 hpf E– embryos. At 24 hpf in E + embryos, Sox10 expressing-neural crest cells are localized both in the central nervous system and dorsal root ganglia (DRG), while the Sox10 signal is diminished in E– embryos in both the DRG and early enteric nervous system. At 24 hpf, Ttpa expression outlines the brain ventricle borders; critically E– embryos show reduced Ttpa signal and impaired ventricle closing. Gene expression by qPCR will be used to confirm these results.

    Conclusions
    This VitE deficient embryo model suggests that the carefully programmed development of the nervous system is distorted due to lack of adequate VitE. Thus, Ttpa and VitE are critical molecules for neural plate and neural tube formation, and neural crest cell migration.

    Funding Sources
    The authors received no specific funding for this work.

  • Modulation of ozone-sensitive genes in Alpha-Tocopherol Transfer Protein null mice.
    Inhalation Toxicology, 2009
    Co-Authors: Vihas T. Vasu, Carroll E. Cross, Brad A. Hobson, Maret G Traber, Saji Oommen, Giuseppe Valacchi, Jason P. Eiserich, Scott W. Leonard, Kishorchandra Gohil

    Abstract:

    Alpha-Tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic Alpha-Tocopherol (AT) deficiency, with their lung AT levels being

  • Alpha-Tocopherol Transfer Protein (α-TTP): Insights from Alpha-Tocopherol Transfer Protein Knockout Mice
    Nutrition Research and Practice, 2007
    Co-Authors: Maret G Traber

    Abstract:

    Alpha-Tocopherol Transfer Protein (α-TTP) is a liver cytosolic transport Protein that faciliates α-tocopherol (α-T) Transfer into liver secreted plasma lipoProteins. Genetic defects in α-TTP, like dietary vitamin E deficiency, are associated with infertility, muscular weakness and neurological disorders. Both human and α-TTP deficient (α-TTP-/-) mice exhibit severe plasma and tissue vitamin E deficiency that can be attenuated by sufficient dietary α-T supplementations. In this review, we summarize the literature concerning studies utilizing the α-TTP-/- mice. Levels of vitamin E in the α-TTP-/- mice do not appear to be directly related to the amounts of dietary α-T or to the levels of α-TTP Protein in tissues. The α-TTP-/- mice appear to present a good model for investigating the specific role of α-T in tissue vitamin E metabolism. Furthermore, α-TTP-/- mice appear to be useful to elucidate functions of α-TTP beyond its well recognized functions of Transferring α-T from liver to plasma lipoProtein fractions.

Carroll E. Cross – 2nd expert on this subject based on the ideXlab platform

  • Modulation of ozone-sensitive genes in Alpha-Tocopherol Transfer Protein null mice.
    Inhalation Toxicology, 2009
    Co-Authors: Vihas T. Vasu, Carroll E. Cross, Brad A. Hobson, Maret G Traber, Saji Oommen, Giuseppe Valacchi, Jason P. Eiserich, Scott W. Leonard, Kishorchandra Gohil

    Abstract:

    Alpha-Tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic Alpha-Tocopherol (AT) deficiency, with their lung AT levels being

  • Genome-Wide Screening of Alpha-Tocopherol Sensitive Genes in Heart Tissue from Alpha-Tocopherol Transfer Protein Null Mice (ATTP−/−)
    FEBS Letters, 2007
    Co-Authors: Vihas T. Vasu, Brad A. Hobson, Kishorchandra Gohil, Carroll E. Cross

    Abstract:

    Abstract Alpha-Tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic deficiency of Alpha-Tocopherol (AT). The heart AT levels of ATTP−/− are

  • Genome‐wide screening of alpha‐tocopherol sensitive genes in heart tissue from alpha‐tocopherol Transfer Protein null mice (ATTP−/−)
    FEBS Letters, 2007
    Co-Authors: Vihas T. Vasu, Brad A. Hobson, Kishorchandra Gohil, Carroll E. Cross

    Abstract:

    Alpha tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic deficiency of Alpha-Tocopherol (AT). The heart AT levels of ATTP−/− are

Kishorchandra Gohil – 3rd expert on this subject based on the ideXlab platform

  • Modulation of ozone-sensitive genes in Alpha-Tocopherol Transfer Protein null mice.
    Inhalation Toxicology, 2009
    Co-Authors: Vihas T. Vasu, Carroll E. Cross, Brad A. Hobson, Maret G Traber, Saji Oommen, Giuseppe Valacchi, Jason P. Eiserich, Scott W. Leonard, Kishorchandra Gohil

    Abstract:

    Alpha-Tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic Alpha-Tocopherol (AT) deficiency, with their lung AT levels being

  • Genome-Wide Screening of Alpha-Tocopherol Sensitive Genes in Heart Tissue from Alpha-Tocopherol Transfer Protein Null Mice (ATTP−/−)
    FEBS Letters, 2007
    Co-Authors: Vihas T. Vasu, Brad A. Hobson, Kishorchandra Gohil, Carroll E. Cross

    Abstract:

    Abstract Alpha-Tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic deficiency of Alpha-Tocopherol (AT). The heart AT levels of ATTP−/− are

  • Genome‐wide screening of alpha‐tocopherol sensitive genes in heart tissue from alpha‐tocopherol Transfer Protein null mice (ATTP−/−)
    FEBS Letters, 2007
    Co-Authors: Vihas T. Vasu, Brad A. Hobson, Kishorchandra Gohil, Carroll E. Cross

    Abstract:

    Alpha tocopherol Transfer Protein (ATTP) null mice (ATTP−/−) have a systemic deficiency of Alpha-Tocopherol (AT). The heart AT levels of ATTP−/− are