The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Siriporn Okonogi - One of the best experts on this subject based on the ideXlab platform.
-
formulation optimization anesthetic activity skin permeation and transportation pathway of Alpinia galanga oil snedds in zebrafish danio rerio
European Journal of Pharmaceutics and Biopharmaceutics, 2021Co-Authors: Nattakanwadee Khumpirapang, Louise Von Gersdorff Jorgensen, Anette Mullertz, Thomas Rades, Siriporn OkonogiAbstract:Abstract Alpinia galanga oil (AGO) has an anesthetic activity but its water insoluble property limits its clinical applications. The aim of the present study was to develop a self-nanoemulsifying drug delivery system of AGO (SNEDDS-AGO) to avoid the use of organic solvent and investigate AGO transportation pathway and anesthetic activity. Three optimized formulations from a contour plots of droplet size; SNEDDS-AGO-1, SNEDDS-AGO-2, and SNEDDS-AGO-3, composed of AGO, Miglyol 812, Cremophor RH 40, Capmul MCM EP, and ethanol at the ratios of 40:10:35:10:5, 40:20:15:20:5, and 60:10:15:10:5, respectively were selected as they possessed different droplet size of 62 ± 0.5, 107 ± 2.8, and 207 ± 4.3 nm, respectively. It was found that the droplet size played an important role in fish anesthesia. SNEDDS-AGO-3 showed the longest anesthetic induction time (270 sec) (p
-
development of microemulsion containing Alpinia galanga oil and its major compounds enhancement of antimicrobial activities
Pharmaceutics, 2021Co-Authors: Nattakanwadee Khumpirapang, Srikanjana Klayraung, Singkome Tima, Siriporn OkonogiAbstract:The aim of the present study was to develop a microemulsion (ME) containing Alpinia galanga oil (AGO), 1,8-cineole (C), or methyl eugenol (M) as an active pharmaceutical ingredient (API) for enhancing their antimicrobial activities. Agar diffusion, broth microdilution, and killing kinetics were used for antimicrobial evaluations. The ME composed of 30% API, 33.4% Tween 80, 16.6% ethanol, and 20% water appeared as translucent systems with droplet size and polydispersity index of 101.1 ± 1.3 nm and 0.3 ± 0.1, 80.9 ± 1.1 nm and 0.4 ± 0.1, and 96.6 ± 2.0 nm and 0.2 ± 0.1 for ME-AGO, ME-C, and ME-M, respectively. These ME formulations showed minimum bacterial concentrations of 3.91-31.25 µg/mL and 50% fungal inhibition concentrations of 1.83 ± 0.27-0.46 ± 0.13 µg/mL, 2-4 times stronger, and faster kinetic killing rate than their respective API alone. Keeping the ME formulations at 4 °C, 25 °C, and 40 °C for 12 weeks did not affect their activities against fungi and Gram-negative bacteria, but the high temperature of 40 °C decreased their activities against Gram-positive bacteria. It is concluded that ME is a promising delivery system for AGO and its major compounds to enhance their water miscibility and antimicrobial activities.
-
in vivo anesthetic effect and mechanism of action of active compounds from Alpinia galanga oil on cyprinus carpio koi carp
Aquaculture, 2018Co-Authors: Nattakanwadee Khumpirapang, Surachai Pikulkaew, Anette Mullertz, Siripat Chaichit, Supat Jiranusornkul, Siriporn OkonogiAbstract:Abstract In the present study, three active compounds of Alpinia galanga oil (AGO); 1,8-cineole (37.43%), 4-allylphenyl acetate (25.97%), and methyl eugenol (5.07%) were investigated for anesthetic effect on Cyprinus carpio (koi carp) and their mechanism of action. The anesthetic effect was evaluated by determining the induction time that the fish needed to reach the surgical stage of anesthesia and subsequent recovery time. Among the three test compounds, methyl eugenol showed the shortest induction time and longest recovery time. The induction times of 4-allylphenyl acetate were longer and the recovery times were shorter than 1,8- cineole. For the mechanism of action, the compounds were investigated by computational docking into the benzodiazepine (BZD) site of the γ-aminobutyric acid type A (GABAA) receptor complex model. Ligand-protein docking interaction, i.e., binding energy (ΔGbind) and inhibitory constant (Ki), were calculated. The result of the computational prediction was related to the experimental anesthetic activity, where methyl eugenol showed the lowest ΔGbind of −6.10 kcal mol−1 and Ki of 33.84 μM, followed by 4-allylphenyl acetate (with ΔGbind of −5.67 kcal mol−1 and Ki of 70.30 μM), and 1,8-cineole (with ΔGbind of −5.39 kcal mol−1 and Ki of 112.40 μM). To this end, a comparison of recovery time of the anesthetized fish using flumazenil, a BZD antagonist, was carried out. The fish exposed to all tested compounds resulted a faster recovery in the water tank with flumazenil than that without flumazenil, confirming the receptor binding site of these three compounds are GABAA receptor interaction. It is concluded that the anesthetic effect of AGO on koi carp is according to the three main active compounds; 1,8-cineole, 4-allylphenyl acetate, and methyl eugenol. Moreover, the mechanism of anesthetic action of these compounds is exerting GABAA receptor interaction via the BZD-binding site.
-
self microemulsifying drug delivery system and nanoemulsion for enhancing aqueous miscibility of Alpinia galanga oil
PLOS ONE, 2017Co-Authors: Nattakanwadee Khumpirapang, Surachai Pikulkaew, Anette Mullertz, Thomas Rades, Siriporn OkonogiAbstract:Alpinia galanga oil (AGO) possesses various activities but low aqueous solubility limits its application particularly in aquatic animals. AGO has powerful activity on fish anesthesia. Ethanol used for enhancing water miscible of AGO always shows severe side effects on fish. The present study explores the development of self-microemulsifying drug delivery systems (SMEDDS) and nanoemulsions (NE) to deliver AGO for fish anesthesia with less or no alcohol. Pseudoternary phase diagrams were constructed to identify the best SMEDDS-AGO formulation, whereas NE-AGO were developed by means of high-energy emulsification. The mean droplet size of the best SMEDDS-AGO was 82 ± 0.5 nm whereas that of NE-AGO was 48 ± 1.6 nm. The anesthetic effect of the developed SMEDDS-AGO and NE-AGO in koi (Cyprinus carpio) was evaluated and compared with AGO ethanolic solution (EtOH-AGO). It was found that the time of induction the fish to reach the surgical stage of anesthesia was dose dependent. NE-AGO showed significantly higher activity than SMEDDS-AGO and EtOH-AGO, respectively. EtOH-AGO caused unwanted hyperactivity in the fish. This side effect did not occur in the fish anesthetized with SMEDDS-AGO and NE-AGO. In conclusion, SMEDDS and NE are promising delivery systems for AGO.
-
effects of Alpinia galanga oil on anesthesia and stress reduction in oreochromis niloticus
Drug discoveries and therapeutics, 2017Co-Authors: Surachai Pikulkaew, Nattakanwadee Khumpirapang, Wasana Chaisri, Siriporn OkonogiAbstract:Oreochromis niloticus (Nile tilapia) is one widely cultured fish in Thailand. Handling processes and transportation causes high stress in Nile tilapia. This study explores anesthetic effect and stress reduction of Alpinia galanga oil (AGO) on Nile tilapia. The anesthetic activity was evaluated by the time for fish induction to anesthesia and full recovery. It was found that the suitable dose of AGO that caused desirable anesthesia of Nile tilapia was 700 mg/L. This dose gave induction and recovery times of approximately 257 and 438 sec, respectively. Blood glucose and plasma cortisol of the fish anesthetized with AGO showed nearly normal levels indicating that the fish stress during handling was not increased. Study on loading densities of fish mimicked general fish transportation and showed that loading density of fish was a crucial factor on fish stress. The highest water quality was found in the lowest loading density of fish. Water containing AGO at a concentration of 150 mg/L showed significantly higher potential for reducing fish activity and water improvement than without AGO. Therefore, AGO is a promising natural edible plant oil for anesthesia in Nile tilapia.
Kaeko Kamei - One of the best experts on this subject based on the ideXlab platform.
-
8 The Potential of galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)
2016Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4 % (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantl
-
Effect of galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)
Aquaculture International, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Lila Ruangpan, Kei Nagata, Kaeko KameiAbstract:Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, galangal ( Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of galangal–ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either galangal extract or trans - p -coumaryl diacetate that had been isolated from galangal rhizome, Pacific white shrimp ( Litopenaeus vannamei ) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both galangal extract and trans - p -coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp.
Maurice R Marshall - One of the best experts on this subject based on the ideXlab platform.
-
phytochemical profiles and antimicrobial and antioxidant activities of greater galangal Alpinia galanga linn swartz flowers
Food Chemistry, 2018Co-Authors: Xixuan Tang, Yavuz Yagiz, Amarat Simonne, Maurice R MarshallAbstract:Abstract Previous studies of galangal (Alpinia galanga) have focussed mostly on rhizomes but seldom on flowers. A comprehensive look at galangal flowers could reveal additional benefits. The chemical composition of galangal flowers was significantly (p
Nattakanwadee Khumpirapang - One of the best experts on this subject based on the ideXlab platform.
-
formulation optimization anesthetic activity skin permeation and transportation pathway of Alpinia galanga oil snedds in zebrafish danio rerio
European Journal of Pharmaceutics and Biopharmaceutics, 2021Co-Authors: Nattakanwadee Khumpirapang, Louise Von Gersdorff Jorgensen, Anette Mullertz, Thomas Rades, Siriporn OkonogiAbstract:Abstract Alpinia galanga oil (AGO) has an anesthetic activity but its water insoluble property limits its clinical applications. The aim of the present study was to develop a self-nanoemulsifying drug delivery system of AGO (SNEDDS-AGO) to avoid the use of organic solvent and investigate AGO transportation pathway and anesthetic activity. Three optimized formulations from a contour plots of droplet size; SNEDDS-AGO-1, SNEDDS-AGO-2, and SNEDDS-AGO-3, composed of AGO, Miglyol 812, Cremophor RH 40, Capmul MCM EP, and ethanol at the ratios of 40:10:35:10:5, 40:20:15:20:5, and 60:10:15:10:5, respectively were selected as they possessed different droplet size of 62 ± 0.5, 107 ± 2.8, and 207 ± 4.3 nm, respectively. It was found that the droplet size played an important role in fish anesthesia. SNEDDS-AGO-3 showed the longest anesthetic induction time (270 sec) (p
-
development of microemulsion containing Alpinia galanga oil and its major compounds enhancement of antimicrobial activities
Pharmaceutics, 2021Co-Authors: Nattakanwadee Khumpirapang, Srikanjana Klayraung, Singkome Tima, Siriporn OkonogiAbstract:The aim of the present study was to develop a microemulsion (ME) containing Alpinia galanga oil (AGO), 1,8-cineole (C), or methyl eugenol (M) as an active pharmaceutical ingredient (API) for enhancing their antimicrobial activities. Agar diffusion, broth microdilution, and killing kinetics were used for antimicrobial evaluations. The ME composed of 30% API, 33.4% Tween 80, 16.6% ethanol, and 20% water appeared as translucent systems with droplet size and polydispersity index of 101.1 ± 1.3 nm and 0.3 ± 0.1, 80.9 ± 1.1 nm and 0.4 ± 0.1, and 96.6 ± 2.0 nm and 0.2 ± 0.1 for ME-AGO, ME-C, and ME-M, respectively. These ME formulations showed minimum bacterial concentrations of 3.91-31.25 µg/mL and 50% fungal inhibition concentrations of 1.83 ± 0.27-0.46 ± 0.13 µg/mL, 2-4 times stronger, and faster kinetic killing rate than their respective API alone. Keeping the ME formulations at 4 °C, 25 °C, and 40 °C for 12 weeks did not affect their activities against fungi and Gram-negative bacteria, but the high temperature of 40 °C decreased their activities against Gram-positive bacteria. It is concluded that ME is a promising delivery system for AGO and its major compounds to enhance their water miscibility and antimicrobial activities.
-
in vivo anesthetic effect and mechanism of action of active compounds from Alpinia galanga oil on cyprinus carpio koi carp
Aquaculture, 2018Co-Authors: Nattakanwadee Khumpirapang, Surachai Pikulkaew, Anette Mullertz, Siripat Chaichit, Supat Jiranusornkul, Siriporn OkonogiAbstract:Abstract In the present study, three active compounds of Alpinia galanga oil (AGO); 1,8-cineole (37.43%), 4-allylphenyl acetate (25.97%), and methyl eugenol (5.07%) were investigated for anesthetic effect on Cyprinus carpio (koi carp) and their mechanism of action. The anesthetic effect was evaluated by determining the induction time that the fish needed to reach the surgical stage of anesthesia and subsequent recovery time. Among the three test compounds, methyl eugenol showed the shortest induction time and longest recovery time. The induction times of 4-allylphenyl acetate were longer and the recovery times were shorter than 1,8- cineole. For the mechanism of action, the compounds were investigated by computational docking into the benzodiazepine (BZD) site of the γ-aminobutyric acid type A (GABAA) receptor complex model. Ligand-protein docking interaction, i.e., binding energy (ΔGbind) and inhibitory constant (Ki), were calculated. The result of the computational prediction was related to the experimental anesthetic activity, where methyl eugenol showed the lowest ΔGbind of −6.10 kcal mol−1 and Ki of 33.84 μM, followed by 4-allylphenyl acetate (with ΔGbind of −5.67 kcal mol−1 and Ki of 70.30 μM), and 1,8-cineole (with ΔGbind of −5.39 kcal mol−1 and Ki of 112.40 μM). To this end, a comparison of recovery time of the anesthetized fish using flumazenil, a BZD antagonist, was carried out. The fish exposed to all tested compounds resulted a faster recovery in the water tank with flumazenil than that without flumazenil, confirming the receptor binding site of these three compounds are GABAA receptor interaction. It is concluded that the anesthetic effect of AGO on koi carp is according to the three main active compounds; 1,8-cineole, 4-allylphenyl acetate, and methyl eugenol. Moreover, the mechanism of anesthetic action of these compounds is exerting GABAA receptor interaction via the BZD-binding site.
-
self microemulsifying drug delivery system and nanoemulsion for enhancing aqueous miscibility of Alpinia galanga oil
PLOS ONE, 2017Co-Authors: Nattakanwadee Khumpirapang, Surachai Pikulkaew, Anette Mullertz, Thomas Rades, Siriporn OkonogiAbstract:Alpinia galanga oil (AGO) possesses various activities but low aqueous solubility limits its application particularly in aquatic animals. AGO has powerful activity on fish anesthesia. Ethanol used for enhancing water miscible of AGO always shows severe side effects on fish. The present study explores the development of self-microemulsifying drug delivery systems (SMEDDS) and nanoemulsions (NE) to deliver AGO for fish anesthesia with less or no alcohol. Pseudoternary phase diagrams were constructed to identify the best SMEDDS-AGO formulation, whereas NE-AGO were developed by means of high-energy emulsification. The mean droplet size of the best SMEDDS-AGO was 82 ± 0.5 nm whereas that of NE-AGO was 48 ± 1.6 nm. The anesthetic effect of the developed SMEDDS-AGO and NE-AGO in koi (Cyprinus carpio) was evaluated and compared with AGO ethanolic solution (EtOH-AGO). It was found that the time of induction the fish to reach the surgical stage of anesthesia was dose dependent. NE-AGO showed significantly higher activity than SMEDDS-AGO and EtOH-AGO, respectively. EtOH-AGO caused unwanted hyperactivity in the fish. This side effect did not occur in the fish anesthetized with SMEDDS-AGO and NE-AGO. In conclusion, SMEDDS and NE are promising delivery systems for AGO.
-
effects of Alpinia galanga oil on anesthesia and stress reduction in oreochromis niloticus
Drug discoveries and therapeutics, 2017Co-Authors: Surachai Pikulkaew, Nattakanwadee Khumpirapang, Wasana Chaisri, Siriporn OkonogiAbstract:Oreochromis niloticus (Nile tilapia) is one widely cultured fish in Thailand. Handling processes and transportation causes high stress in Nile tilapia. This study explores anesthetic effect and stress reduction of Alpinia galanga oil (AGO) on Nile tilapia. The anesthetic activity was evaluated by the time for fish induction to anesthesia and full recovery. It was found that the suitable dose of AGO that caused desirable anesthesia of Nile tilapia was 700 mg/L. This dose gave induction and recovery times of approximately 257 and 438 sec, respectively. Blood glucose and plasma cortisol of the fish anesthetized with AGO showed nearly normal levels indicating that the fish stress during handling was not increased. Study on loading densities of fish mimicked general fish transportation and showed that loading density of fish was a crucial factor on fish stress. The highest water quality was found in the lowest loading density of fish. Water containing AGO at a concentration of 150 mg/L showed significantly higher potential for reducing fish activity and water improvement than without AGO. Therefore, AGO is a promising natural edible plant oil for anesthesia in Nile tilapia.
Torranis Ruttanaphan - One of the best experts on this subject based on the ideXlab platform.
-
a novel insecticidal molecule extracted from Alpinia galanga with potential to control the pest insect spodoptera frugiperda
Insects, 2020Co-Authors: Anchulee Pengsook, Wanchai Pluempanupat, Vasakorn Bullangpoti, Torranis Ruttanaphan, Georges De Sousa, Hannahisadora Huditz, Gaelle Le GoffAbstract:Spodoptera frugiperda, a highly polyphagous insect pest from America, has recently invaded and widely spread throughout Africa and Asia. Effective and environmentally safe tools are needed for successful pest management of this invasive species. Natural molecules extracted from plants offer this possibility. Our study aimed to determine the insecticidal efficacy of a new molecule extracted from Alpinia galanga rhizome, the 1'S-1'-acetoxychavicol acetate (ACA). The toxicity of ACA was assessed by topical application on early third-instar larvae of S. frugiperda. Results showed that ACA caused significant larval growth inhibition and larval developmental abnormalities. In order to further explore the effects of this molecule, experiments have been performed at the cellular level using Sf9 model cells. ACA exhibited higher toxicity on Sf9 cells as compared to azadirachtin and was 38-fold less toxic on HepG2 cells. Inhibition of cell proliferation was observed at sublethal concentrations of ACA and was associated with cellular morphological changes and nuclear condensation. In addition, ACA induced caspase-3 activity. RT-qPCR experiments reveal that ACA induces the expression of several caspase genes. This first study on the effects of ACA on S. frugiperda larvae and cells provides evidence that ACA may have potential as a botanical insecticide for the control of S. frugiperda.