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Barry M Pryor - One of the best experts on this subject based on the ideXlab platform.

  • identification of Alternaria species causing heart rot of pomegranates in california
    Plant Disease, 2017
    Co-Authors: Y Luo, Barry M Pryor, L Hou, Helga Forster, J E Adaskaveg
    Abstract:

    Alternaria heart rot of pomegranate fruit is generally considered a minor disease. However, the current listing of the causal pathogen in California as “Alternaria sp.” or as “A. alternata and other Alternaria spp.” restricts trade of the crop to some major export markets where quarantines are in place in an effort to prevent the spread of unknown or undescribed pathogens. Thus, species identification of the pathogen is critical in determining whether infected fruit should be regulated by quarantines. In this study, 86 isolates of Alternaria were collected from pomegranate fruit with Alternaria heart rot symptoms from major production areas in California. An unweighted pair group method with arithmetic means analysis based on amplified fragment length polymorphisms revealed two main clusters, each with a high degree of variability. One of the clusters contained 24 isolates from pomegranate and one reference isolate of Alternaria arborescens. Reference isolates of A. alternata and A. tenuissima were found ...

  • The sections of Alternaria: formalizing species-group concepts.
    Mycologia, 2013
    Co-Authors: Daniel P. Lawrence, Philipp B. Gannibal, Tobin L. Peever, Barry M Pryor
    Abstract:

    The systematics of Alternaria and allied genera traditionally has been based on the character- istics of conidia and the sporulation apparatus. This emphasis on morphology in the reconstruction of organismal relationships has resulted in taxonomic uncertainty and flux for a number of taxa in Alternaria and the related genera Stemphylium, Embellisia, Nimbya and Ulocladium. The present study used a molecular phylogenetic approach for system- atic resolution and incorporated extensive taxon sampling (n 5 176 species) representing 10 genera and analyses of 10 protein-coding loci. Phylogenetic analyses based on five of these genes revealed eight distinct asexual lineages of Alternaria that cluster as the sister group to the asexual paraphyletic genus Ulocladium, while taxa with known teleomorphs currently circumscribed as Alternaria (the infectoria species-group) cluster among genera that also have representatives with known teleomorphs. This work proposes to elevate the eight well supported asexual lineages of Alternaria to the taxonomic rank of section. Evolutionary relationships among Alternaria and closely related genera are discussed.

  • Ulocladium systematics revisited: phylogeny and taxonomic status
    Mycological Progress, 2008
    Co-Authors: Farhana Runa, Myung Soo Park, Barry M Pryor
    Abstract:

    The genus Ulocladium represents phaeodictyosporic Hyphomycetes that produce conidia that are essentially obovoid in shape. Previous molecular studies that included Ulocladium and related taxa in Alternaria, Embellisia, and Stemphylium revealed a conflict between morphology and phylogeny, and Ulocladium was supported as polyphyletic with a paraphyletic core group. Moreover, the genus consistently resolved within a larger Alternaria/Ulocladium clade, resulting in paraphyly of Alternaria and questions as to the taxonomic status of Ulocladium. In the present study, 13 Ulocladium species and three genetic loci were included for a more comprehensive systematic analysis of the genus than had previously been conducted. Total genomic DNA was extracted from representative taxa and sequences were determined for the nuclear internal transcribed spacer region, including the 5.8S rDNA gene, and the protein-coding genes glyceraldehyde-3-phosphate dehydrogenase and Alt a1. Subsequent phylogenetic analyses based on maximum parsimony and Bayesian methods included related Alternaria, Embellisia, and Stemphylium spp. Results supported previous findings of polyphyletic and paraphyletic relationships of Ulocladium among other taxa. Ten Ulocladium species clustered into a core Ulocladium clade and all taxa possessed the key diagnostic feature of Ulocladium, namely, conidia essentially obovoid in shape. However, A. cheiranthi and E. indefessa also clustered within this group with high bootstrap support but did not posses this diagnostic feature. This paraphyletic clade resolved basal to the core Alternaria clade with high bootstrap support, unlike previous studies in which its position was imbedded within the primary Alternaria clade. Thus, the status of the genus as an independent lineage and a unique taxon is strongly supported. As previously reported, U. Alternariae and U. oudemansii, which posses the key conidium characteristics of Ulocladium, clustered as a separate clade sister to the core Ulocladium clade. Further studies are necessary to determine if these taxa represent an independent lineage or share a common ancestor with other Ulocladium species. Obovoid conidia were poorly represented in the isolate of U. lanuginosum that was included in these analyses (the only U. lanuginosum isolate currently available), and the isolate resolved as A. radicina based upon all three loci sequenced. Based upon these data and the origin of the isolate, which was originally deposited as A. malvae, a reassessment of its identity is supported.

  • molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear its and mt ssu rdna sequences
    Fungal Biology, 2000
    Co-Authors: Barry M Pryor, R L Gilbertson
    Abstract:

    To elucidate relationships among Alternaria, Ulocladium, and Stemphylium species, nuclear internal transcribed spacer (ITS) and mitochondrial small subunit (SSU) ribosomal DNA (rDNA) sequences from 18 Alternaria, four Ulocladium and four Stemphylium spp. were determined and compared. Phylogenetic analysis of the ITS and SSU rDNA sequences, performed by the neighbour joining and maximum parsimony methods, revealed that the Stemphylium spp. were phylogenetically distinct from the Alternaria and Ulocladium spp. Most Alternaria spp. and the Ulocladium spp. were placed together in a large Alternaria/Ulocladium clade. Within this large clade, the Alternaria spp. clustered into several distinct species-clades, most of which correlated with species-groups previously established based upon morphological characteristics. The Ulocladium spp. were placed into two species-clades, each of which also included Alternaria spp. A. longissima was distantly related to the other Alternaria spp., as well as the Ulocladium and Stemphylium spp. Based upon ITS and nuclear 18S rDNA sequence identities, A. longissima was most closely related to Leptosphaeria.

Ian Benywanira - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Sweetpotato (Ipomoea batatas (L.) Lam.) Genotypes for Resistance to Alternaria Leaf Petiole and Stem Blight (Alternaria spp.) in Uganda
    Journal of Agricultural Science, 2020
    Co-Authors: G. Sseruwu, Mary Nanyanzi, Agnes Alajo, George Kituuka, Ian Benywanira
    Abstract:

    Alternaria leaf petiole and stem blight (Alternaria spp.) is an important sweetpotato (Ipomoea batatas (L.) Lam.) disease in Uganda. Severity of the disease varies with environment, with higher disease levels recorded under high moisture and humidity conditions. To breed for resistance to this disease, germplasm that is resistant must be identified through multi-locational trials. This study was conducted to evaluate selected sweetpotato genotypes for stable resistance to Alternaria blight across sites and seasons. Thirty sweetpotato genotypes from different agro-ecological zones of Uganda and the National Sweetpotato Program were evaluated for resistance to Alternaria blight using fungicide treatment and Alternaria blight pathogen inoculation at Namulonge and Kachwekano over three seasons. There were highly significant differences among the genotypes for Alternaria blight severity with higher disease levels at Kachwekano than Namulonge. Genotypes Shock, Silk Luwero and the resistant check Tanzania had the lowest Alternaria severity and were therefore the most resistant while NASPOT 1 and NASPOT 7 had the highest severity values and were the most susceptible. Improved cultivars were more susceptible than the landraces. Genotypes Tanzania and Namusoga and environment Namulonge 2015B were the most stable for Alternaria blight. Treatment with fungicide resulted in variable reductions in Alternaria blight severity among genotypes across seasons and sites with NASPOT 1 having the lowest percentage reduction of 40.8% between the Alternaria inoculated and fungicide treated plots. Kigaire recorded the highest percentage disease reduction of 63.6%. Those genotypes with acceptable performance for Alternaria blight may be used as parents in breeding new genotypes with improved performance.

G. Sseruwu - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Sweetpotato (Ipomoea batatas (L.) Lam.) Genotypes for Resistance to Alternaria Leaf Petiole and Stem Blight (Alternaria spp.) in Uganda
    Journal of Agricultural Science, 2020
    Co-Authors: G. Sseruwu, Mary Nanyanzi, Agnes Alajo, George Kituuka, Ian Benywanira
    Abstract:

    Alternaria leaf petiole and stem blight (Alternaria spp.) is an important sweetpotato (Ipomoea batatas (L.) Lam.) disease in Uganda. Severity of the disease varies with environment, with higher disease levels recorded under high moisture and humidity conditions. To breed for resistance to this disease, germplasm that is resistant must be identified through multi-locational trials. This study was conducted to evaluate selected sweetpotato genotypes for stable resistance to Alternaria blight across sites and seasons. Thirty sweetpotato genotypes from different agro-ecological zones of Uganda and the National Sweetpotato Program were evaluated for resistance to Alternaria blight using fungicide treatment and Alternaria blight pathogen inoculation at Namulonge and Kachwekano over three seasons. There were highly significant differences among the genotypes for Alternaria blight severity with higher disease levels at Kachwekano than Namulonge. Genotypes Shock, Silk Luwero and the resistant check Tanzania had the lowest Alternaria severity and were therefore the most resistant while NASPOT 1 and NASPOT 7 had the highest severity values and were the most susceptible. Improved cultivars were more susceptible than the landraces. Genotypes Tanzania and Namusoga and environment Namulonge 2015B were the most stable for Alternaria blight. Treatment with fungicide resulted in variable reductions in Alternaria blight severity among genotypes across seasons and sites with NASPOT 1 having the lowest percentage reduction of 40.8% between the Alternaria inoculated and fungicide treated plots. Kigaire recorded the highest percentage disease reduction of 63.6%. Those genotypes with acceptable performance for Alternaria blight may be used as parents in breeding new genotypes with improved performance.

  • Genetic analysis of resistance to Alternaria leaf petiole and stem blight of sweetpotato in Uganda
    Euphytica, 2016
    Co-Authors: G. Sseruwu, P. Shanahan, R. Melis, G. Ssemakula
    Abstract:

    Alternaria blight ( Alternaria spp.) is an important sweetpotato disease in Uganda causing yield losses of over 50 % in susceptible genotypes. In Uganda, Alternaria bataticola and Alternaria alternata are the major species with A . bataticola the more aggressive of the two. The most effective control measure for this disease is the use of resistant genotypes. This study was conducted to determine the inheritance of resistance to Alternaria blight and the general and specific combining abilities of the available germplasm. Sixteen parental clones varying in reaction to Alternaria blight were crossed using the North Carolina II mating scheme. Due to incompatibility of some parents, two sets of compatible parents were formed. Differences among the families for Alternaria blight severity were significant while general combining ability (GCA) and specific combining ability (SCA) mean squares were highly significant (P 

Jagreet Kaur - One of the best experts on this subject based on the ideXlab platform.

  • Comparative genomics of Alternaria species provides insights into the pathogenic lifestyle of Alternaria brassicae - a pathogen of the Brassicaceae family.
    BMC genomics, 2019
    Co-Authors: Sivasubramanian Rajarammohan, Kumar Paritosh, Deepak Pental, Jagreet Kaur
    Abstract:

    Alternaria brassicae, a necrotrophic pathogen, causes Alternaria Leaf Spot, one of the economically important diseases of Brassica crops. Many other Alternaria spp. such as A. brassicicola and A. alternata are known to cause secondary infections in the A. brassicae-infected Brassicas. The genome architecture, pathogenicity factors, and determinants of host-specificity of A. brassicae are unknown. In this study, we annotated and characterised the recently announced genome assembly of A. brassicae and compared it with other Alternaria spp. to gain insights into its pathogenic lifestyle. We also sequenced the genomes of two A. alternata isolates that were co-infecting B. juncea using Nanopore MinION sequencing for additional comparative analyses within the Alternaria genus. Genome alignments within the Alternaria spp. revealed high levels of synteny between most chromosomes with some intrachromosomal rearrangements. We show for the first time that the genome of A. brassicae, a large-spored Alternaria species, contains a dispensable chromosome. We identified 460 A. brassicae-specific genes, which included many secreted proteins and effectors. Furthermore, we have identified the gene clusters responsible for the production of Destruxin-B, a known pathogenicity factor of A. brassicae. The study provides a perspective into the unique and shared repertoire of genes within the Alternaria genus and identifies genes that could be contributing to the pathogenic lifestyle of A. brassicae.

  • Comparative genomics of Alternaria species provides insights into the pathogenic lifestyle of Alternaria brassicae – a pathogen of the Brassicaceae family
    2019
    Co-Authors: Sivasubramanian Rajarammohan, Kumar Paritosh, Deepak Pental, Jagreet Kaur
    Abstract:

    Abstract Alternaria brassicae, a necrotrophic pathogen, causes Alternaria Leaf Spot, one of the economically important diseases of Brassica crops. Many other Alternaria spp. such as A. brassicicola and A. alternata are known to cause secondary infections in the A. brassicae-infected Brassicas. The genome architecture, pathogenicity factors, and determinants of host-specificity of A. brassicae are unknown. In this study, we annotated and characterised the recently announced genome assembly of A. brassicae and compared it with other Alternaria spp. to gain insights into its pathogenic lifestyle. Additionally, we sequenced the genomes of two A. alternata isolates that were co-infecting B. juncea. Genome alignments within the Alternaria spp. revealed high levels of synteny between most chromosomes with some intrachromosomal rearrangements. We show for the first time that the genome of A. brassicae, a large-spored Alternaria species, contains a dispensable chromosome. We identified 460 A. brassicae-specific genes, which included many secreted proteins and effectors. Furthermore, we have identified the gene clusters responsible for the production of Destruxin-B, a known pathogenicity factor of A. brassicae. The study provides a perspective into the unique and shared repertoire of genes within the Alternaria genus and identifies genes that could be contributing to the pathogenic lifestyle of A. brassicae.

Amira Sh. Soliman - One of the best experts on this subject based on the ideXlab platform.

  • Phylogenetic, toxigenic and virulence profiles of Alternaria species causing leaf blight of tomato in Egypt
    Mycological Progress, 2018
    Co-Authors: Samah Fawzy Gobashy, Wafai Z. A. Mikhail, Ahmed Mahmoud Ismail, Adel Zekry, Anotonio Moretti, Antonella Susca, Amira Sh. Soliman
    Abstract:

    Species of Alternaria are serious plant pathogens, causing major losses on a wide range of crops. Leaf blight symptoms were observed on tomato leaves, and samples were collected from various regions. Isolation was done from symptomatic tomato leaves, and 15 representatives were selected from a collection of 65 isolates of Alternaria species. The virulence of Alternaria isolates was investigated on detached leaves (DL) and whole plants of tomato cv. Super strain B. A phylogenetic analysis was performed based on three partial gene regions, the glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ), the RNA polymerase second largest subunit ( RPB2 ) and the Alternaria major allergen gene ( Alt a 1 ). The potentiality of Alternaria isolates to produce toxins was also investigated on the basis of thin-layer chromatography (TLC). Our investigations revealed that Alternaria isolates showed different levels of virulence either on tomato plants or DL. Based on the phylogeny of three genes, Alternaria isolates encompassed two species of small-spored morphospecies: A . alternata (14 isolates) and A . arborescens (single isolate). The produced toxins varied among Alternaria isolates with tenuazonic acid (TeA) being the most abundant mycotoxin produced by most isolates. This study highlighted on other Alternaria species in Egypt that might represent a serious concern for tomato producers as causal agents of leaf blight over other species, i.e. A. solani .