The Experts below are selected from a list of 474 Experts worldwide ranked by ideXlab platform

Charles P. Scutt - One of the best experts on this subject based on the ideXlab platform.

  • correction to transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    The article Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells, written by Maria Flores-Tornero, Sebastian Proost, Marek Mutwil, Charles P. Scutt, Thomas Dresselhaus, Stefanie Sprunck, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 1 February 2019 without open access

  • Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Plant Reproduction, 2019
    Co-Authors: María Flores-tornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    Key message A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Abstract Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella -specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

  • transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella-specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

  • evidence for the extensive conservation of mechanisms of ovule integument development since the most recent common ancestor of living angiosperms
    Frontiers in Plant Science, 2018
    Co-Authors: Gontran Arnault, Aurelie C M Vialette, Amelie Andresrobin, Gildas Gâteble, Bruno Fogliani, Charles P. Scutt
    Abstract:

    The ovules and seeds of most angiosperm groups are enclosed by two integuments, whose evolutionary origins are considerably separated in time, as the inner integument arose over 300 million years ago (MYA) in an ancestor of all living seed plants, while the outer integument arose, perhaps as recently as 164 MYA, in an ancestor of all living angiosperms. Studies of the model angiosperm Arabidopsis thaliana indicate that the mechanisms of development of the inner and outer integuments depend on largely different sets of molecular players. However, it was not known, in most cases, whether these differences were already present in early flowering plants, or arose later in the Arabidopsis lineage. Here, we analyze the expression patterns of integument regulators in Amborella trichopoda, the likely sister to all other living angiosperms. The data obtained indicate that regulators of the YABBY, KANADI, and homeodomain-leucine zipper class III transcription factor families have largely conserved their integument-specific expression profiles in the Amborella and Arabidopsis lineages since the most recent common ancestor (MRCA) of living angiosperms. We identified only one case, involving the paralogous genes ETTIN and AUXIN RESPONSE FACTOR4, in which integument-specific expression patterns had clearly diverged between Amborella and Arabidopsis. We use the data obtained to partially reconstruct molecular mechanisms of integument development in the MRCA of living angiosperms and discuss our findings in the context of alternative hypotheses for the origin of the angiosperm outer integument.

  • The morphophysiological dormancy in Amborella trichopoda seeds is a pleisiomorphic trait in angiosperms
    Annals of Botany, 2017
    Co-Authors: Bruno Fogliani, Gildas Gâteble, M. Villegente, Carol C Baskin, Isabelle Fabre, Nicolas Klein, Nicolas Anger, Charles P. Scutt
    Abstract:

    Background and Aims Recent parsimony-based reconstructions suggest that seeds of early angiosperms had either morphophysiological or physiological dormancy, with the former considered as more probable. The aim of this study was to determine the class of seed dormancy present in Amborella trichopoda, the sole living representative of the most basal angiosperm lineage Amborellales, with a view to resolving fully the class of dormancy present at the base of the angiosperm clade. Methods Drupes of A. trichopoda without fleshy parts were germinated and dissected to observe their structure and embryo growth. Pre-treatments including acid scarification, gibberellin treatment and seed excision were tested to determine their influence on dormancy breakage and germination. Character-state mapping by maximum parsimony, incorporating data from the present work and published sources, was then used to determine the likely class of dormancy present in early angiosperms. Key Results Germination in A. trichopoda requires a warm stratification period of at least approx. 90 d, which is followed by endosperm swelling, causing the water-permeable pericarp-mesocarp envelope to split open. The embryo then grows rapidly within the seed, to radicle emergence some 17 d later and cotyledon emergence after an additional 24 d. Gibberellin treatment, acid scarification and excision of seeds from the surrounding drupe tissues all promoted germination by shortening the initial phase of dormancy, prior to embryo growth. Conclusions Seeds of A. trichopoda have non-deep simple morphophysiological dormancy, in which mechanical resistance of the pericarp-mesocarp envelope plays a key role in the initial physiological phase. Maximum parsimony analyses, including data obtained in the present work, indicate that morphophysiological dormancy is likely to be a pleisiomorphic trait in flowering plants. The significance of this conclusion for studies of early angiosperm evolution is discussed.

Thomas Dresselhaus - One of the best experts on this subject based on the ideXlab platform.

  • transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions
    Plant Physiology, 2020
    Co-Authors: Maria Florestornero, Marek Mutwil, Stefanie Sprunck, Frank Vogler, David Potěsil, Ivana Ihnatova, Zbyněk Zdrahal, Thomas Dresselhaus
    Abstract:

    Flowering plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel toward the ovules, where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent PT functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated rna-sequencing/">RNA sequencing data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella trichopoda and complemented these with proteomic data of pollen grains (PGs) and PTs. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bicellular A. trichopoda PGs, to induce their germination in vitro, and to monitor PT growth and germ cell division. Transcriptomic and proteomic analyses indicate that A. trichopoda PGs are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while PTs are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis, and the number of genes involved in pollen signaling is significantly reduced in A. trichopoda In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

  • transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions
    Plant Physiology, 2020
    Co-Authors: Maria Florestornero, Marek Mutwil, Stefanie Sprunck, Frank Vogler, David Potěsil, Ivana Ihnatova, Zbyněk Zdrahal, Thomas Dresselhaus
    Abstract:

    Flowering plants (angiosperms) are characterized by pollen tubes (male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel towards the ovules where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent pollen tube functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated RNA-seq data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella (Amborella trichopoda) and complemented these with proteomic data of pollen grains and pollen tubes. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bi-cellular Amborella pollen grains, to induce their germination in vitro, and to monitor pollen tube growth and germ cell division. Transcriptomic and proteomic analyses indicate that Amborella pollen grains are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while pollen tubes are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis and the number of genes involved in pollen signaling is significantly reduced in Amborella. In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

  • correction to transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    The article Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells, written by Maria Flores-Tornero, Sebastian Proost, Marek Mutwil, Charles P. Scutt, Thomas Dresselhaus, Stefanie Sprunck, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 1 February 2019 without open access

  • Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Plant Reproduction, 2019
    Co-Authors: María Flores-tornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    Key message A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Abstract Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella -specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

  • transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella-specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

Stefanie Sprunck - One of the best experts on this subject based on the ideXlab platform.

  • transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions
    Plant Physiology, 2020
    Co-Authors: Maria Florestornero, Marek Mutwil, Stefanie Sprunck, Frank Vogler, David Potěsil, Ivana Ihnatova, Zbyněk Zdrahal, Thomas Dresselhaus
    Abstract:

    Flowering plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel toward the ovules, where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent PT functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated rna-sequencing/">RNA sequencing data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella trichopoda and complemented these with proteomic data of pollen grains (PGs) and PTs. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bicellular A. trichopoda PGs, to induce their germination in vitro, and to monitor PT growth and germ cell division. Transcriptomic and proteomic analyses indicate that A. trichopoda PGs are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while PTs are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis, and the number of genes involved in pollen signaling is significantly reduced in A. trichopoda In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

  • transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions
    Plant Physiology, 2020
    Co-Authors: Maria Florestornero, Marek Mutwil, Stefanie Sprunck, Frank Vogler, David Potěsil, Ivana Ihnatova, Zbyněk Zdrahal, Thomas Dresselhaus
    Abstract:

    Flowering plants (angiosperms) are characterized by pollen tubes (male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel towards the ovules where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent pollen tube functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated RNA-seq data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella (Amborella trichopoda) and complemented these with proteomic data of pollen grains and pollen tubes. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bi-cellular Amborella pollen grains, to induce their germination in vitro, and to monitor pollen tube growth and germ cell division. Transcriptomic and proteomic analyses indicate that Amborella pollen grains are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while pollen tubes are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis and the number of genes involved in pollen signaling is significantly reduced in Amborella. In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

  • correction to transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    The article Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells, written by Maria Flores-Tornero, Sebastian Proost, Marek Mutwil, Charles P. Scutt, Thomas Dresselhaus, Stefanie Sprunck, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 1 February 2019 without open access

  • Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Plant Reproduction, 2019
    Co-Authors: María Flores-tornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    Key message A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Abstract Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella -specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

  • transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella-specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

Marek Mutwil - One of the best experts on this subject based on the ideXlab platform.

  • transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions
    Plant Physiology, 2020
    Co-Authors: Maria Florestornero, Marek Mutwil, Stefanie Sprunck, Frank Vogler, David Potěsil, Ivana Ihnatova, Zbyněk Zdrahal, Thomas Dresselhaus
    Abstract:

    Flowering plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel toward the ovules, where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent PT functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated rna-sequencing/">RNA sequencing data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella trichopoda and complemented these with proteomic data of pollen grains (PGs) and PTs. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bicellular A. trichopoda PGs, to induce their germination in vitro, and to monitor PT growth and germ cell division. Transcriptomic and proteomic analyses indicate that A. trichopoda PGs are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while PTs are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis, and the number of genes involved in pollen signaling is significantly reduced in A. trichopoda In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

  • transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions
    Plant Physiology, 2020
    Co-Authors: Maria Florestornero, Marek Mutwil, Stefanie Sprunck, Frank Vogler, David Potěsil, Ivana Ihnatova, Zbyněk Zdrahal, Thomas Dresselhaus
    Abstract:

    Flowering plants (angiosperms) are characterized by pollen tubes (male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel towards the ovules where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent pollen tube functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated RNA-seq data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella (Amborella trichopoda) and complemented these with proteomic data of pollen grains and pollen tubes. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bi-cellular Amborella pollen grains, to induce their germination in vitro, and to monitor pollen tube growth and germ cell division. Transcriptomic and proteomic analyses indicate that Amborella pollen grains are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while pollen tubes are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis and the number of genes involved in pollen signaling is significantly reduced in Amborella. In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

  • correction to transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    The article Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells, written by Maria Flores-Tornero, Sebastian Proost, Marek Mutwil, Charles P. Scutt, Thomas Dresselhaus, Stefanie Sprunck, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 1 February 2019 without open access

  • Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Plant Reproduction, 2019
    Co-Authors: María Flores-tornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    Key message A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Abstract Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella -specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

  • transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
    Sexual Plant Reproduction, 2019
    Co-Authors: Maria Florestornero, Charles P. Scutt, Sebastian Proost, Marek Mutwil, Thomas Dresselhaus, Stefanie Sprunck
    Abstract:

    A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella-specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.

Bruno Fogliani - One of the best experts on this subject based on the ideXlab platform.

  • a derived zw chromosome system in Amborella trichopoda representing the sister lineage to all other extant flowering plants
    New Phytologist, 2021
    Co-Authors: Jos Kafer, Amelie Andresrobin, Gildas Gâteble, Bruno Fogliani, Alex Harkess, Adam J Bewick, Garance Lapetoule, Jose Caius, Paula E Ralph
    Abstract:

    The genetic basis and evolution of sex determination in dioecious plants is emerging as an active area of research with exciting advances in genome sequencing and analysis technologies. As the sole species within the sister lineage to all other extant flowering plants, Amborella trichopoda is an important model for understanding the evolution and development of flowers. Plants typically produce only male or female flowers, but sex determination mechanisms are unknown for the species. Sequence data derived from plants of natural origin and an F1 mapping population were used to identify sex-linked genes and the nonrecombining region. Amborella trichopoda has a ZW sex determination system. Analysis of genes in a 4 Mb nonrecombining sex-determination region reveals recent divergence of Z and W gametologs, and few Z- and W-specific genes. The sex chromosomes of A. trichopoda evolved less than 16.5 Myr ago, long after the divergence of the extant angiosperms.

  • a derived zw chromosome system in Amborella trichopoda the sister species to all other extant flowering plants
    bioRxiv, 2020
    Co-Authors: Jos Kafer, Amelie Andresrobin, Gildas Gâteble, Bruno Fogliani, Alex Harkess, Adam J Bewick, Garance Lapetoule, Jose Caius, Paula E Ralph, Claude W. Depamphilis
    Abstract:

    Sex determination is poorly understood in plants. Amborella trichopoda is a well-known plant model for evo-devo studies, which is also dioecious (has male and female individuals), with an unknown sex determination mechanism. A. trichopoda is a sex switcher, which points to possible environmental factors that act on sex, but populations grown from seed under greenhouse conditions exhibit a 50:50 sex ratio, which indicates the operation of genetic factors. Here, we use a new method (SDpop) to identify sex-linked genes from genotyping data of male and female individuals sampled in the field, and find that A. trichopoda has a ZW sex-chromosome system. The sex-linked genes map to a 4 Mb sex-determining region on chromosome 9. The low extent of ZW divergence suggests these sex chromosomes are of recent origin, which is consistent with dioecy being derived character in the A. trichopoda lineage. Our work has uncovered clearly formed sex chromosomes in a species in which both genetic and environmental factors can influence sex.

  • Amborella bearing witness to the past
    Annual Plant Reviews Online, 2019
    Co-Authors: Valerie Poncet, Gildas Gâteble, Bruno Fogliani, Tanguy Jaffré, Alexandre De ,kochko, Philippe Birnbaum, Valerie Burtetsarramegna, Sandrine Isnard, Dominique Job
    Abstract:

    Amborella trichopoda (Amborellaceae) is a shrub endemic to New Caledonia in the Southwest Pacific region. This plant suddenly became famous when molecular phylogenetic studies revealed that this sole species is likely the sister taxon to all other angiosperms. It has thus been a prime research model for reconstructing plant evolution and gaining insight into what the earliest angiosperms looked like. A wealth of studies on Amborella have now shed considerable light on its genome, morphology, anatomy, physiology, development, and architecture – this research is reviewed in this article. While Amborella likely retained some ancestral traits, critical character reconstructions have also highlighted some derived and sometimes unique characters in this species. The history of Amborella is also tied to the South Pacific archipelago of New Caledonia, its homeland. It was part of the New Caledonian biogeography puzzle and its genetic history shed light on the dynamics of its ecosystem, the rainforest understorey. Amborella is now cultivated in botanical gardens and has been the focus of some conservation measures that will also benefit other species in this biodiversity hotspot.

  • evidence for the extensive conservation of mechanisms of ovule integument development since the most recent common ancestor of living angiosperms
    Frontiers in Plant Science, 2018
    Co-Authors: Gontran Arnault, Aurelie C M Vialette, Amelie Andresrobin, Gildas Gâteble, Bruno Fogliani, Charles P. Scutt
    Abstract:

    The ovules and seeds of most angiosperm groups are enclosed by two integuments, whose evolutionary origins are considerably separated in time, as the inner integument arose over 300 million years ago (MYA) in an ancestor of all living seed plants, while the outer integument arose, perhaps as recently as 164 MYA, in an ancestor of all living angiosperms. Studies of the model angiosperm Arabidopsis thaliana indicate that the mechanisms of development of the inner and outer integuments depend on largely different sets of molecular players. However, it was not known, in most cases, whether these differences were already present in early flowering plants, or arose later in the Arabidopsis lineage. Here, we analyze the expression patterns of integument regulators in Amborella trichopoda, the likely sister to all other living angiosperms. The data obtained indicate that regulators of the YABBY, KANADI, and homeodomain-leucine zipper class III transcription factor families have largely conserved their integument-specific expression profiles in the Amborella and Arabidopsis lineages since the most recent common ancestor (MRCA) of living angiosperms. We identified only one case, involving the paralogous genes ETTIN and AUXIN RESPONSE FACTOR4, in which integument-specific expression patterns had clearly diverged between Amborella and Arabidopsis. We use the data obtained to partially reconstruct molecular mechanisms of integument development in the MRCA of living angiosperms and discuss our findings in the context of alternative hypotheses for the origin of the angiosperm outer integument.

  • A Combination of Histological, Physiological, and Proteomic Approaches Shed Light on Seed Desiccation Tolerance of the Basal Angiosperm Amborella trichopoda.
    Proteomes, 2017
    Co-Authors: M. Villegente, G. Cueff, L. Rajjou, T. Balliau, M. Zivy, Philippe Marmey, Claudette Job, Marc Galland, Beatrice Godin, Bruno Fogliani
    Abstract:

    Desiccation tolerance allows plant seeds to remain viable in a dry state for years and even centuries. To reveal potential evolutionary processes of this trait, we have conducted a shotgun proteomic analysis of isolated embryo and endosperm from mature seeds of Amborella trichopoda, an understory shrub endemic to New Caledonia that is considered to be the basal extant angiosperm. The present analysis led to the characterization of 415 and 69 proteins from the isolated embryo and endosperm tissues, respectively. The role of these proteins is discussed in terms of protein evolution and physiological properties of the rudimentary, underdeveloped, Amborella embryos, notably considering that the acquisition of desiccation tolerance corresponds to the final developmental stage of mature seeds possessing large embryos.