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Kurunthachalam Kannan - One of the best experts on this subject based on the ideXlab platform.
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Determination of melamine and its derivatives in textiles and infant clothing purchased in the United States.
The Science of the total environment, 2019Co-Authors: Hongkai Zhu, Kurunthachalam KannanAbstract:Melamine-based resins are used extensively in fabrics to impart fire and heat resistance as well as wrinkle-free properties. Little is known, however, regarding the occurrence of melamine and its derivatives in textiles. In this study, concentrations of melamine, Ammeline, ammelide, and cyanuric acid were determined in 77 textile samples and infant clothing purchased from Albany, New York, USA. All textile samples contained one or more target analytes, at concentration ranges of 1.19-81,800, 3.21-17,800,
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Occurrence and Profiles of Melamine and Cyanuric Acid in Bovine Feed and Urine from China, India, and the United States.
Environmental science & technology, 2019Co-Authors: Hongkai Zhu, Bommanna G. Loganathan, Kurunthachalam KannanAbstract:Melamine and cyanuric acid have been reported to occur in animal products. Nevertheless, information that pertains to the occurrence of melamine and cyanuric acid in cattle feed and urine is lacking. In this study, the occurrence of melamine and its three derivatives (i.e., cyanuric acid, Ammeline, and ammelide) was determined in 183 bovine urine and 29 matched feed samples collected from China, India, and the United States. ∑Melamine (sum of four target compounds) was found in all urine samples at concentrations that ranged from 4.2 to 5280 ng/mL (median: 370 ng/mL); cyanuric acid was the major derivative, accounting for 97% of the total concentrations, followed by melamine (2.2%). The ubiquitous occurrence of ∑Melamine in feed (21–6230 ng/g) suggests that it is the major source of melamine and its derivatives in bovines. Urinary concentrations of melamine and cyanuric acid varied significantly among the three countries, with samples from China as having the highest concentrations, followed by the United...
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Occurrence and Profiles of Melamine and Cyanuric Acid in Bovine Feed and Urine from China, India, and the United States
2019Co-Authors: Hongkai Zhu, Bommanna G. Loganathan, Kurunthachalam KannanAbstract:Melamine and cyanuric acid have been reported to occur in animal products. Nevertheless, information that pertains to the occurrence of melamine and cyanuric acid in cattle feed and urine is lacking. In this study, the occurrence of melamine and its three derivatives (i.e., cyanuric acid, Ammeline, and ammelide) was determined in 183 bovine urine and 29 matched feed samples collected from China, India, and the United States. ∑Melamine (sum of four target compounds) was found in all urine samples at concentrations that ranged from 4.2 to 5280 ng/mL (median: 370 ng/mL); cyanuric acid was the major derivative, accounting for 97% of the total concentrations, followed by melamine (2.2%). The ubiquitous occurrence of ∑Melamine in feed (21–6230 ng/g) suggests that it is the major source of melamine and its derivatives in bovines. Urinary concentrations of melamine and cyanuric acid varied significantly among the three countries, with samples from China as having the highest concentrations, followed by the United States and India. The calculated cumulative daily intakes of melamine and cyanuric acid were at least 10-fold below the current tolerable daily intake recommended for humans. Our study provides evidence-based data on exposure patterns and sources of melamine and cyanuric acid in cattle
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Inter-day and inter-individual variability in urinary concentrations of melamine and cyanuric acid
Environment international, 2018Co-Authors: Hongkai Zhu, Kurunthachalam KannanAbstract:Abstract Melamine is used extensively in household products, such as furniture, dinnerware, and food utensils. Several studies have shown that melamine adversely affects kidney function. Nevertheless, little is known about urinary melamine concentrations, and its temporal variability. In this study, 213 first-morning-void urine samples were collected from 19 volunteers for over a month to assess longitudinal variability in concentrations of melamine and its three structural analogues, i.e., cyanuric acid, Ammeline, and ammelide. Target analytes were found in all urine samples at mean concentrations of 3.3, 16, 0.99, and 0.62 ng/mL, for melamine, cyanuric acid, ammelide, and Ammeline, respectively. Cyanuric acid was the major compound found in all urine samples, accounting for 74–80% of the total concentrations, followed by melamine (12–20%), ammelide (4–6%), and Ammeline (2–4%). Gender- and age-related differences in melamine concentrations were observed, although no such pattern was found for cyanuric acid. After adjusting for creatinine, melamine and cyanuric acid concentrations were moderately predictable with inter-day intraclass correlation coefficients (ICCs) in the range of 0.541–0.763. Nevertheless, substantial inter-individual variation in melamine levels existed even after creatinine adjustment, as evidenced by low ICCs (0.008–0.108). Cumulative daily intake of melamine and cyanuric acid was calculated on the basis of urinary concentrations and the mean values were found to be at least 10-fold below the current tolerable daily intake.
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Distribution Profiles of Melamine and Its Derivatives in Indoor Dust from 12 Countries and the Implications for Human Exposure
Environmental science & technology, 2018Co-Authors: Hongkai Zhu, Kurunthachalam KannanAbstract:Melamine and its derivatives (i.e., Ammeline, ammelide, and cyanuric acid) are extensively used in household products, such as furniture, dinnerware, and food utensils. Nevertheless, limited information is available on human exposures, other than dietary sources, to melamine and its derivatives. In this study, the occurrence of melamine and its derivatives was determined, for the first time, in 341 samples of indoor dust, collected from 12 countries. All of the dust samples contained target analytes, with global median concentrations of 1800, 1100, 48, and 45 ng/g for melamine, cyanuric acid, ammelide, and Ammeline, respectively. The total concentrations of melamine and its derivatives varied among countries in the following decreasing order: United States (median: 17 000 ng/g) > Japan (8400) > China (8000) > South Korea (7300) > Saudi Arabia (4400) > Romania (4300) > Greece (3400) > Kuwait (2400) > Vietnam (2300) > Colombia (1300) > Pakistan (820) > India (430). Correlation analysis indicated the existen...
Hanwen Sun - One of the best experts on this subject based on the ideXlab platform.
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rapid screening and quantification of cyromazine melamine ammelide Ammeline cyanuric acid and dicyandiamide in infant formula by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry and triple quadrupole ma
Food Control, 2015Co-Authors: Shuxuan Liang, Zhe Meng, Zhihong Shi, Xinfeng Dong, Hanwen SunAbstract:Abstract In the past few years, special concern for triazine pesticide cyromazine (CYRO) and its metabolites melamine (MM), ammelide (AMD), Ammeline (AMN), cyanuric acid (CA), and dicyandiamide (DCD) residues has arisen in the food safety field on infant foods. In this work, screening and confirmation for infant milk powder samples were performed by quadrupole time-of-flight mass spectrometry (QTOF-MS). An accurate mass database was established. The matrix-matched internal standard calibration curves of UPLC–MS/MS were linear, with correlation coefficient (r2) higher than 0.994. The limits of quantification (LOQ) were 1.6, 5.0, 16.5, 10.0, 66.4, and 66.4 μg/kg for CYRO, MM, AMD, AMN, CA, and DCD, respectively. The average recoveries ranged from 64.8 to 103%. The retention time, intra- and inter-day precisions (relative standard deviation, RSDs) of 6 analytes were in the range of 0.02–0.05%, 2.4–6.1%, and 7.2–11.2%, respectively. CYRO and MM residues in some infant milk powder samples were confirmed. Quantitation of positive samples was performed using the internal standard method and six-point matrix-matched calibration curves. The content for CYRO in all 8 samples and MM in 4 samples were in the range of 3.5–45 μg/kg and 8–25 μg/kg, respectively. The method developed for the first time has high sensitivity, can be used as an effective tool for rapid screening and highly sensitive multiresidue determination of CYRO, MM and their metabolites AMD, AMN, and CA as well as DCD in milk powder samples.
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highly sensitive determination of cyromazine melamine and their metabolites in milk by molecularly imprinted solid phase extraction combined with ultra performance liquid chromatography
Journal of Dairy Science, 2015Co-Authors: Junmin Wang, Shuxuan Liang, Hanwen SunAbstract:A novel molecularly imprinted solid-phase extraction-ultra-performance liquid chromatography (MISPE-UPLC) method for effective separation and simultaneous determination of cyromazine, melamine, and their metabolites (Ammeline and ammelide) in milk samples was developed. Molecularly imprinted polymers (MIP) were synthesized in an ethanol-water system, with melamine as the template and methacrylic acid as the organic functional monomer. The MIP were applied as a specific sorbent for the selective solid phase extraction of cyromazine, ammelide, melamine and Ammeline. The molecular recognition mechanism was investigated by molecular simulation and the experiment was validate by using Fourier transform infrared spectroscopy and (1)H nuclear magnetic resonance spectroscopy. A new mechanism based on the formation of both an amido group and hydrogen bonds was developed. A binding study demonstrated that the MIP showed excellent affinity to and high selectivity for melamine and related compounds. Under optimized conditions, we achieved good linearity of the calibration curves with correlation coefficients >0.999. Low limits of quantification (LOQ) for the method were determined to be 1.25, 1.25, 2.59, and 6.42 µg/kg for cyromazine, ammelide, melamine, and Ammeline, respectively, which were 3 orders of magnitude smaller than the maximum residue limit (MRL). The high sensitivity of this method allows detection at the microgram per kilogram level. The proposed MISPE-UPLC method is a highly selective and sensitive method for determination of cyromazine, melamine, and their metabolites (Ammeline and ammelide) for use in the control and quality assurance of milk.
Robert H Poppenga - One of the best experts on this subject based on the ideXlab platform.
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diagnostic determination of melamine and related compounds in kidney tissue by liquid chromatography tandem mass spectrometry
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Michael S Filigenzi, Birgit Puschner, Linda S Aston, Robert H PoppengaAbstract:In 2007, it was determined that melamine, Ammeline, ammelide, and cyanuric acid (abbreviated as MARC for melamine and related contaminants) had been added to wheat gluten and rice protein that were subsequently incorporated into pet food. The consumption of food tainted by MARC compounds was implicated in numerous instances of renal failure in cats and dogs. A method for the analysis of MARC compounds in kidney tissue using high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) has been developed. MARC analytes were extracted by homogenization of kidney tissue in 50/40/10 acetonitrile/water/diethylamine. The homogenate was centrifuged, and an aliquot of supernatant was diluted with acetonitrile, concentrated, and fortified with a stable isotope-labeled analogue of melamine. Analytes were detected using atmospheric pressure chemical ionization and multiple reaction monitoring. Quantitation of positive samples was performed using the internal standard method and five-point calibration ...
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diagnostic determination of melamine and related compounds in kidney tissue by liquid chromatography tandem mass spectrometry
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Michael S Filigenzi, Birgit Puschner, Linda S Aston, Robert H PoppengaAbstract:In 2007, it was determined that melamine, Ammeline, ammelide, and cyanuric acid (abbreviated as MARC for melamine and related contaminants) had been added to wheat gluten and rice protein that were subsequently incorporated into pet food. The consumption of food tainted by MARC compounds was implicated in numerous instances of renal failure in cats and dogs. A method for the analysis of MARC compounds in kidney tissue using high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) has been developed. MARC analytes were extracted by homogenization of kidney tissue in 50/40/10 acetonitrile/water/diethylamine. The homogenate was centrifuged, and an aliquot of supernatant was diluted with acetonitrile, concentrated, and fortified with a stable isotope-labeled analogue of melamine. Analytes were detected using atmospheric pressure chemical ionization and multiple reaction monitoring. Quantitation of positive samples was performed using the internal standard method and five-point calibration curves ranging between 50 and 1000 ng/mL of each analyte. The method was validated by analysis of replicate kidney tissue samples fortified with the individual analytes and by analysis of kidney samples fortified with melamine cyanurate powder at two different concentrations. This method was successfully used for routine postmortem diagnosis of melamine toxicosis in animals. Melamine was also detected by this method in paraffin-embedded tissue from animals suspected to have died of melamine toxicosis.
Zhe Meng - One of the best experts on this subject based on the ideXlab platform.
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rapid screening and quantification of cyromazine melamine ammelide Ammeline cyanuric acid and dicyandiamide in infant formula by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry and triple quadrupole ma
Food Control, 2015Co-Authors: Shuxuan Liang, Zhe Meng, Zhihong Shi, Xinfeng Dong, Hanwen SunAbstract:Abstract In the past few years, special concern for triazine pesticide cyromazine (CYRO) and its metabolites melamine (MM), ammelide (AMD), Ammeline (AMN), cyanuric acid (CA), and dicyandiamide (DCD) residues has arisen in the food safety field on infant foods. In this work, screening and confirmation for infant milk powder samples were performed by quadrupole time-of-flight mass spectrometry (QTOF-MS). An accurate mass database was established. The matrix-matched internal standard calibration curves of UPLC–MS/MS were linear, with correlation coefficient (r2) higher than 0.994. The limits of quantification (LOQ) were 1.6, 5.0, 16.5, 10.0, 66.4, and 66.4 μg/kg for CYRO, MM, AMD, AMN, CA, and DCD, respectively. The average recoveries ranged from 64.8 to 103%. The retention time, intra- and inter-day precisions (relative standard deviation, RSDs) of 6 analytes were in the range of 0.02–0.05%, 2.4–6.1%, and 7.2–11.2%, respectively. CYRO and MM residues in some infant milk powder samples were confirmed. Quantitation of positive samples was performed using the internal standard method and six-point matrix-matched calibration curves. The content for CYRO in all 8 samples and MM in 4 samples were in the range of 3.5–45 μg/kg and 8–25 μg/kg, respectively. The method developed for the first time has high sensitivity, can be used as an effective tool for rapid screening and highly sensitive multiresidue determination of CYRO, MM and their metabolites AMD, AMN, and CA as well as DCD in milk powder samples.
Shuxuan Liang - One of the best experts on this subject based on the ideXlab platform.
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rapid screening and quantification of cyromazine melamine ammelide Ammeline cyanuric acid and dicyandiamide in infant formula by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry and triple quadrupole ma
Food Control, 2015Co-Authors: Shuxuan Liang, Zhe Meng, Zhihong Shi, Xinfeng Dong, Hanwen SunAbstract:Abstract In the past few years, special concern for triazine pesticide cyromazine (CYRO) and its metabolites melamine (MM), ammelide (AMD), Ammeline (AMN), cyanuric acid (CA), and dicyandiamide (DCD) residues has arisen in the food safety field on infant foods. In this work, screening and confirmation for infant milk powder samples were performed by quadrupole time-of-flight mass spectrometry (QTOF-MS). An accurate mass database was established. The matrix-matched internal standard calibration curves of UPLC–MS/MS were linear, with correlation coefficient (r2) higher than 0.994. The limits of quantification (LOQ) were 1.6, 5.0, 16.5, 10.0, 66.4, and 66.4 μg/kg for CYRO, MM, AMD, AMN, CA, and DCD, respectively. The average recoveries ranged from 64.8 to 103%. The retention time, intra- and inter-day precisions (relative standard deviation, RSDs) of 6 analytes were in the range of 0.02–0.05%, 2.4–6.1%, and 7.2–11.2%, respectively. CYRO and MM residues in some infant milk powder samples were confirmed. Quantitation of positive samples was performed using the internal standard method and six-point matrix-matched calibration curves. The content for CYRO in all 8 samples and MM in 4 samples were in the range of 3.5–45 μg/kg and 8–25 μg/kg, respectively. The method developed for the first time has high sensitivity, can be used as an effective tool for rapid screening and highly sensitive multiresidue determination of CYRO, MM and their metabolites AMD, AMN, and CA as well as DCD in milk powder samples.
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highly sensitive determination of cyromazine melamine and their metabolites in milk by molecularly imprinted solid phase extraction combined with ultra performance liquid chromatography
Journal of Dairy Science, 2015Co-Authors: Junmin Wang, Shuxuan Liang, Hanwen SunAbstract:A novel molecularly imprinted solid-phase extraction-ultra-performance liquid chromatography (MISPE-UPLC) method for effective separation and simultaneous determination of cyromazine, melamine, and their metabolites (Ammeline and ammelide) in milk samples was developed. Molecularly imprinted polymers (MIP) were synthesized in an ethanol-water system, with melamine as the template and methacrylic acid as the organic functional monomer. The MIP were applied as a specific sorbent for the selective solid phase extraction of cyromazine, ammelide, melamine and Ammeline. The molecular recognition mechanism was investigated by molecular simulation and the experiment was validate by using Fourier transform infrared spectroscopy and (1)H nuclear magnetic resonance spectroscopy. A new mechanism based on the formation of both an amido group and hydrogen bonds was developed. A binding study demonstrated that the MIP showed excellent affinity to and high selectivity for melamine and related compounds. Under optimized conditions, we achieved good linearity of the calibration curves with correlation coefficients >0.999. Low limits of quantification (LOQ) for the method were determined to be 1.25, 1.25, 2.59, and 6.42 µg/kg for cyromazine, ammelide, melamine, and Ammeline, respectively, which were 3 orders of magnitude smaller than the maximum residue limit (MRL). The high sensitivity of this method allows detection at the microgram per kilogram level. The proposed MISPE-UPLC method is a highly selective and sensitive method for determination of cyromazine, melamine, and their metabolites (Ammeline and ammelide) for use in the control and quality assurance of milk.