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  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess

    Abstract:

    Zusammenfassung

    Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme.

    Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar.

    Summary

    The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis

    The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America.

    The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation.

    Resume

    La formation et l’histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf

    La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie.

    La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu’au Soudan III n’est qu’une forme de presentation dependant de la fixation du noyau cellulaire soumis a l’action du virus.

    Resumen

    Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno

    La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess

    Abstract:

    Zusammenfassung

    Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme.

    Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar.

    Summary

    The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis

    The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America.

    The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation.

    Resume

    La formation et l’histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf

    La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie.

    La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu’au Soudan III n’est qu’une forme de presentation dependant de la fixation du noyau cellulaire soumis a l’action du virus.

    Resumen

    Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno

    La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

H. Schimmelpfennig – One of the best experts on this subject based on the ideXlab platform.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess

    Abstract:

    Zusammenfassung

    Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme.

    Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar.

    Summary

    The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis

    The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America.

    The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation.

    Resume

    La formation et l’histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf

    La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie.

    La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu’au Soudan III n’est qu’une forme de presentation dependant de la fixation du noyau cellulaire soumis a l’action du virus.

    Resumen

    Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno

    La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess

    Abstract:

    Zusammenfassung

    Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme.

    Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar.

    Summary

    The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis

    The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America.

    The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation.

    Resume

    La formation et l’histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf

    La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie.

    La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu’au Soudan III n’est qu’une forme de presentation dependant de la fixation du noyau cellulaire soumis a l’action du virus.

    Resumen

    Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno

    La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

Maria João Moreno – One of the best experts on this subject based on the ideXlab platform.

  • kinetics and thermodynamics of association of a phospholipid derivative with lipid bilayers in liquid disordered and liquid ordered phases
    Biophysical Journal, 2004
    Co-Authors: Magda S C Abreu, Maria João Moreno

    Abstract:

    We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described method that exploits a detailed knowledge of the binding kinetics of an amphiphile to bovine serum albumin, to recover the insertion and desorption rate constants when the albumin-bound amphiphile is transferred through the aqueous phase to the membrane and vice versa. The lipid bilayers, studied as large unilamellar vesicles, were prepared from pure 1-palmitoyl-2-oleoylphosphatidylcholine in the liquid-disordered phase; and from two cholesterol-containing binary lipid mixtures, 1-palmitoyl-2-oleoylphosphatidylcholine and cholesterol (molar ratio of 1:1), and egg sphingomyelin and cholesterol (molar ratio of 6:4), both in the liquid-ordered phase. Insertion, desorption, and translocation rate constants and equilibrium constants for association of the amphiphile monomer with the lipid bilayers were directly measured between 15° and 35°C, and the standard free energies, enthalpies, and entropies, as well as the activation energies for these processes, were derived from this data. The equilibrium partition coefficients for partitioning of the amphiphile between the aqueous phase and the different membrane phases were also derived, and permitted the estimation of hypothetical partition coefficients and the respective energetic parameters for partitioning between the different lipid phases if these were to coexist in the same membrane.