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B Liess - One of the best experts on this subject based on the ideXlab platform.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess
    Abstract:

    Zusammenfassung Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme. Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar. Summary The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America. The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation. Resume La formation et l'histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie. La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu'au Soudan III n'est qu'une forme de presentation dependant de la fixation du noyau cellulaire soumis a l'action du virus. Resumen Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess
    Abstract:

    Zusammenfassung Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme. Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar. Summary The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America. The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation. Resume La formation et l'histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie. La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu'au Soudan III n'est qu'une forme de presentation dependant de la fixation du noyau cellulaire soumis a l'action du virus. Resumen Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

H. Schimmelpfennig - One of the best experts on this subject based on the ideXlab platform.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess
    Abstract:

    Zusammenfassung Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme. Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar. Summary The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America. The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation. Resume La formation et l'histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie. La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu'au Soudan III n'est qu'une forme de presentation dependant de la fixation du noyau cellulaire soumis a l'action du virus. Resumen Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

  • Entwicklung und Histochemie der Kerneinschlußkörperchen in Gewebekulturen aus Rinderhodenzellen bei der Rhinotracheitis infectiosa des Rindes
    Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2010
    Co-Authors: H. Schimmelpfennig, B Liess
    Abstract:

    Zusammenfassung Der in Westdeutschland kulturell isolierte Rhinotracheitisvirusstamm B1 verursacht in der Gewebekultur gleichartige Veranderungen wie mehrere von anderer Seite untersuchte amerikanische Rhinotracheitisvirusstamme. Die Entwicklung der Amphophilen, Feulgen-positiven, im Brachet-Test, der Perjodsaure-Schiff-Reaktion sowie in der Sudan III-Farbung negativen Kerneinschluskorperchen (Typ A) verlauft ahnlich wie beim Herpesvirus uber mehrere Vorstadien. Das fertige Kerneinschluskorperchen stellt nur eine fixationsabhangige Darstellungsart des der Viruseinwirkung unterliegenden Zellkerns dar. Summary The development and histochemistry of nuclear inclusions in tissue cultures of bovine testes produced by the virus of infectious bovine rhinotracheitis The strain of infectious rhinotracheitis B isolated in Western Germany in culture produces similar changes in tissue culture to a variety of other strains of the virus isolated in America. The formation of Amphophile inclusions which are Feulgen-positive but are negative to the Brachet test, P.A.S. and Sudan III is similar to what happens with herpes virus in the early stages. The final inclusions in the nuclei represent merely the effect of the virus demonstrated by different methods of fixation. Resume La formation et l'histochimie des inclusions nucleaires dans les cultures de cellules testiculaires bovines dans la rhinotracheite infectieuse du boeuf La souche de virus de la rhino-tracheite infectieuse B1 isolee en Allemagne par culture provoque dans la culture de tissu les memes lesions que plusieurs souches etudiees ailleurs du virus americain de la maladie. La formation des inclusions nucleaires Amphophiles se colorant par le Feulgen mais negatifs au test de Brachet, a la reaction acide periodique-Schiff ainsi qu'au Soudan III n'est qu'une forme de presentation dependant de la fixation du noyau cellulaire soumis a l'action du virus. Resumen Desarrollo e histoquimica de los corpusculos de inclusion nuclear en cultivos histicos de celulas testiculares bovinas en la rinotraqueitis infecciosa del ganado vacuno La cepa B1 del virus de la rinotraqueitis, aislada por medio de cultivo en la Republica Federal Alemana, ocasiona en el cultivo histico alteraciones similares a varias cepas americanas del virus de la rinotraqueitis estudiadas por otros autores. El desarrollo de los corpusculos de inclusion nuclear amfofilos, Feulgen-positivos, negativos en la prueba de Brachet, en la reaccion del acido peryodico de Schiff, asi como en la coloracion con Sudan III, solo resulta ser una forma representativa dependiente de la fijacion del nucleo celular sometido a la accion del virus.

Maria João Moreno - One of the best experts on this subject based on the ideXlab platform.

  • kinetics and thermodynamics of association of a phospholipid derivative with lipid bilayers in liquid disordered and liquid ordered phases
    Biophysical Journal, 2004
    Co-Authors: Magda S C Abreu, Maria João Moreno
    Abstract:

    We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described method that exploits a detailed knowledge of the binding kinetics of an amphiphile to bovine serum albumin, to recover the insertion and desorption rate constants when the albumin-bound amphiphile is transferred through the aqueous phase to the membrane and vice versa. The lipid bilayers, studied as large unilamellar vesicles, were prepared from pure 1-palmitoyl-2-oleoylphosphatidylcholine in the liquid-disordered phase; and from two cholesterol-containing binary lipid mixtures, 1-palmitoyl-2-oleoylphosphatidylcholine and cholesterol (molar ratio of 1:1), and egg sphingomyelin and cholesterol (molar ratio of 6:4), both in the liquid-ordered phase. Insertion, desorption, and translocation rate constants and equilibrium constants for association of the amphiphile monomer with the lipid bilayers were directly measured between 15° and 35°C, and the standard free energies, enthalpies, and entropies, as well as the activation energies for these processes, were derived from this data. The equilibrium partition coefficients for partitioning of the amphiphile between the aqueous phase and the different membrane phases were also derived, and permitted the estimation of hypothetical partition coefficients and the respective energetic parameters for partitioning between the different lipid phases if these were to coexist in the same membrane.

Trevor Yann - One of the best experts on this subject based on the ideXlab platform.

Samuel I Stupp - One of the best experts on this subject based on the ideXlab platform.

  • Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres.
    Nature Communications, 2016
    Co-Authors: Ricardo M. P. Da Silva, Daan Van Der Zwaag, Lorenzo Albertazzi, E. W. Meijer, Samuel I Stupp
    Abstract:

    The dynamic behaviour of supramolecular systems is an important dimension of their potential functions. Here, we report on the use of stochastic optical reconstruction microscopy to study the molecular exchange of peptide amphiphile nanofibres, supramolecular systems known to have important biomedical functions. Solutions of nanofibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes inserting into initially single-colour nanofibres was quantified using correlative image analysis. Our observations are consistent with an exchange mechanism involving monomers or small clusters of molecules inserting randomly into a fibre. Different exchange rates are observed within the same fibre, suggesting that local cohesive structures exist on the basis of β-sheet discontinuous domains. The results reported here show that peptide amphiphile supramolecular systems can be dynamic and that their intermolecular interactions affect exchange patterns. This information can be used to generate useful aggregate morphologies for improved biomedical function.

  • Pathway selection in peptide amphiphile assembly
    Journal of the American Chemical Society, 2014
    Co-Authors: Peter A. Korevaar, Christina J. Newcomb, E. W. Meijer, Samuel I Stupp
    Abstract:

    The nature of supramolecular structures could be strongly affected by the pathways followed during their formation just as mechanisms and final outcomes in chemical reactions vary with the conditions selected. So far this is a largely unexplored area of supramolecular chemistry. We demonstrate here how different preparation protocols to self-assemble peptide amphiphiles in water can result in the formation of different supramolecular morphologies, either long filaments containing β-sheets or smaller aggregrates containing peptide segments in random coil conformation. We found that the assembly rate into β-sheets decreases in the presence of a destabilizing “good” solvent like hexafluoroisopropanol (HFIP) and is affected by transient conditions in solution. Also the peptide amphiphile investigated spontaneously nucleates the β-sheet-containing filaments at a critical fraction of HFIP in water below 21%. Furthermore, β-sheet assemblies have a high kinetic stability and, once formed, do not disassemble rapid...

  • supramolecular nanofibers of peptide amphiphiles for medicine
    Israel Journal of Chemistry, 2013
    Co-Authors: Matthew J. Webber, Eric J Berns, Samuel I Stupp
    Abstract:

    : Peptide nanostructures are an exciting class of supramolecular systems that can be designed for novel therapies with great potential in advanced medicine. This paper reviews progress on nanostructures based on peptide amphiphiles capable of forming one-dimensional assemblies that emulate in structure the nanofibers present in extracellular matrices. These systems are highly tunable using supramolecular chemistry, and can be designed to signal cells directly with bioactive peptides. Peptide amphiphile nanofibers can also be used to multiplex functions through co-assembly and designed to deliver proteins, nucleic acids, drugs, or cells. We illustrate here the functionality of these systems describing their use in regenerative medicine of bone, cartilage, the nervous system, the cardiovascular system, and other tissues. In addition, we highlight recent work on the use of peptide amphiphile assemblies to create hierarchical biomimetic structures with order beyond the nanoscale, and also discuss the future prospects of these supramolecular systems.

  • Bioinspired Magnetite Mineralization of Peptide−Amphiphile Nanofibers
    Chemistry of Materials, 2011
    Co-Authors: Eli D. Sone, Samuel I Stupp
    Abstract:

    Peptide−amphiphile nanofibers displaying iron-binding sequences were used as templates to control the growth and organization of magnetite (Fe3O4) nanocrystals into linear arrays, mimicking aspects of the linear arrangement of magnetite crystals along a filamentous structure in magnetotactic bacteria.

  • Peptide amphiphile nanostructure-heparin interactions and their relationship to bioactivity.
    Biomaterials, 2008
    Co-Authors: Kanya Rajangam, Michael S. Arnold, Mark A. Rocco, Samuel I Stupp
    Abstract:

    Abstract Heparin–protein interactions are important in many physiological processes including angiogenesis, the growth of new blood vessels from existing ones. We have previously developed a highly angiogenic self-assembling gel, wherein the self-assembly process is triggered by the interactions between heparin and peptide amphiphiles (PAs) with a consensus heparin binding sequence. In this report, this consensus sequence was scrambled and incorporated into a new peptide amphiphile in order to study its importance in heparin interaction and bioactivity. Heparin was able to trigger gel formation of the scrambled peptide amphiphile (SPA). Furthermore, the affinity of the scrambled molecule for heparin was unchanged as shown by isothermal titration calorimetry and high Forster resonance emission transfer efficiency. However, both the mobile fraction and the dissociation rate constant of heparin, using fluorescence recovery after photobleaching, were markedly higher in its interaction with the scrambled molecule implying a weaker association. Importantly, the scrambled peptide amphiphile–heparin gel had significantly less angiogenic bioactivity as shown by decreased tubule formation of sandwiched endothelial cells. Hence, we believe that the presence of the consensus sequence stabilizes the interaction with heparin and is important for the bioactivity of these new materials.

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