The Experts below are selected from a list of 204 Experts worldwide ranked by ideXlab platform
M. Teresa Galceran - One of the best experts on this subject based on the ideXlab platform.
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Field amplified sample injection‐capillary zone electrophoresis for the analysis of Amprolium in eggs
Electrophoresis, 2013Co-Authors: Anna Martínez-villalba, Oscar Núñez, Encarnación Moyano, M. Teresa GalceranAbstract:Veterinary medicines are widely administered to farm animals since they keep animals healthy at overcrowded conditions. Nevertheless the continuous administration of medicines to farm animals can frequently lead to the presence of residues of veterinary drugs in consumption products. Amprolium is a quaternary ammonium compound used in the treatment of coccidiosis. In this paper, a method based on CZE to analyze residues of Amprolium in eggs was developed and validated for the first time. Parameters such as electrolyte type, concentration, and pH were optimized. In order to improve sensitivity, field-amplified sample injection (FASI) was used for in-line preconcentration after a quick and simple sample treatment based on SPE (Envi-Carb). During method-validation studies using egg samples, a matrix interference was found at the migration time of Amprolium. This compound was identified as thiamine and confirmed by MS(n) experiments using CE coupled to MS (CE-MS) with an ion-trap mass analyzer. CZE conditions were reoptimized to separate thiamine from Amprolium allowing the quantification of Amprolium in eggs at concentrations down to 75 μg/kg, which are far below the MRL-legislated values.
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Analysis of Amprolium by hydrophilic interaction liquid chromatography–tandem mass spectrometry
Journal of chromatography. A, 2010Co-Authors: Anna Martínez-villalba, Encarnación Moyano, M. Teresa GalceranAbstract:We present a fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of the coccidiostat Amprolium in food samples. Tandem mass spectrometry in a triple quadrupole was used for quantitative purposes, and the information from multiple-stage mass spectrometry in an ion-trap mass analyzer contributed to fragmentation studies. Hydrophilic interaction liquid chromatography (HILIC) in a Fused-Core column using isocratic elution (acetonitrile:formic acid/ammonium formate buffer pH 4, 50 mM (60:40)) successfully analyzed this compound in less than 3 min. The HILIC system was coupled to heated electrospray-MS/MS using highly selective-selected reaction monitoring (H-SRM) to improve sensitivity and selectivity for the analysis of Amprolium, after a simple sample treatment based on an "extract and shoot" strategy. Accurate mass measurements were performed to identify the interfering compound responsible for causing matrix ion enhancement in the signal of Amprolium. The addition of l-carnitine (the interfering compound) (1 microg L(-1)) to standards and sample extracts allowed the use of the external calibration method for quantitative purposes. The LC-MS/MS (H-SRM) method showed good precision (relative standard deviation, RSD, lower than 13%), accuracy and linearity and allowed the determination of Amprolium down to the ppb level (LODs between 0.1 and 0.6 microg kg(-1)).
Teresa M Galceran - One of the best experts on this subject based on the ideXlab platform.
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field amplified sample injection capillary zone electrophoresis for the analysis of Amprolium in eggs
Electrophoresis, 2013Co-Authors: Anna Martinezvillalba, Oscar Núñez, Encarnación Moyano, Teresa M GalceranAbstract:Veterinary medicines are widely administered to farm animals since they keep animals healthy at overcrowded conditions. Nevertheless the continuous administration of medicines to farm animals can frequently lead to the presence of residues of veterinary drugs in consumption products. Amprolium is a quaternary ammonium compound used in the treatment of coccidiosis. In this paper, a method based on CZE to analyze residues of Amprolium in eggs was developed and validated for the first time. Parameters such as electrolyte type, concentration, and pH were optimized. In order to improve sensitivity, field-amplified sample injection (FASI) was used for in-line preconcentration after a quick and simple sample treatment based on SPE (Envi-Carb). During method-validation studies using egg samples, a matrix interference was found at the migration time of Amprolium. This compound was identified as thiamine and confirmed by MS(n) experiments using CE coupled to MS (CE-MS) with an ion-trap mass analyzer. CZE conditions were reoptimized to separate thiamine from Amprolium allowing the quantification of Amprolium in eggs at concentrations down to 75 μg/kg, which are far below the MRL-legislated values.
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analysis of Amprolium by hydrophilic interaction liquid chromatography tandem mass spectrometry
Journal of Chromatography A, 2010Co-Authors: Anna Martinezvillalba, Encarnación Moyano, Teresa M GalceranAbstract:We present a fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of the coccidiostat Amprolium in food samples. Tandem mass spectrometry in a triple quadrupole was used for quantitative purposes, and the information from multiple-stage mass spectrometry in an ion-trap mass analyzer contributed to fragmentation studies. Hydrophilic interaction liquid chromatography (HILIC) in a Fused-Core column using isocratic elution (acetonitrile:formic acid/ammonium formate buffer pH 4, 50 mM (60:40)) successfully analyzed this compound in less than 3 min. The HILIC system was coupled to heated electrospray-MS/MS using highly selective-selected reaction monitoring (H-SRM) to improve sensitivity and selectivity for the analysis of Amprolium, after a simple sample treatment based on an "extract and shoot" strategy. Accurate mass measurements were performed to identify the interfering compound responsible for causing matrix ion enhancement in the signal of Amprolium. The addition of l-carnitine (the interfering compound) (1 microg L(-1)) to standards and sample extracts allowed the use of the external calibration method for quantitative purposes. The LC-MS/MS (H-SRM) method showed good precision (relative standard deviation, RSD, lower than 13%), accuracy and linearity and allowed the determination of Amprolium down to the ppb level (LODs between 0.1 and 0.6 microg kg(-1)).
M.c. Abete - One of the best experts on this subject based on the ideXlab platform.
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determination of Amprolium in feed by a liquid chromatography mass spectrometry method
Journal of Pharmaceutical and Biomedical Analysis, 2008Co-Authors: S. Squadrone, C. Mauro, Gian Luca Ferro, Giuseppina Amato, M.c. AbeteAbstract:As a consequence of the finding of veterinarian drugs in food European Community banned several compounds like coccidiostats as Amprolium (APL). This antibiotic has been used as a preventive and clinical anticoccidial drug in chicken. The 2005/187/CE, 2005/925/EC Recommendations ban the use of Amprolium as additive in chicken feed. For this reason a rapid and sensitive liquid chromatography–mass spectrometry (LC–MS) method was developed to detect Amprolium in chicken feed following the European community proposed technique (1999/27/EC) for sample preparation. Cause the validation is required for the analytical methods used in feed official control, this method was validated according to 2004/882/EC Regulation.
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Determination of Amprolium in feed by a liquid chromatography–mass spectrometry method
Journal of pharmaceutical and biomedical analysis, 2008Co-Authors: S. Squadrone, C. Mauro, Gian Luca Ferro, Giuseppina Amato, M.c. AbeteAbstract:As a consequence of the finding of veterinarian drugs in food European Community banned several compounds like coccidiostats as Amprolium (APL). This antibiotic has been used as a preventive and clinical anticoccidial drug in chicken. The 2005/187/CE, 2005/925/EC Recommendations ban the use of Amprolium as additive in chicken feed. For this reason a rapid and sensitive liquid chromatography–mass spectrometry (LC–MS) method was developed to detect Amprolium in chicken feed following the European community proposed technique (1999/27/EC) for sample preparation. Cause the validation is required for the analytical methods used in feed official control, this method was validated according to 2004/882/EC Regulation.
Encarnación Moyano - One of the best experts on this subject based on the ideXlab platform.
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field amplified sample injection capillary zone electrophoresis for the analysis of Amprolium in eggs
Electrophoresis, 2013Co-Authors: Anna Martinezvillalba, Oscar Núñez, Encarnación Moyano, Teresa M GalceranAbstract:Veterinary medicines are widely administered to farm animals since they keep animals healthy at overcrowded conditions. Nevertheless the continuous administration of medicines to farm animals can frequently lead to the presence of residues of veterinary drugs in consumption products. Amprolium is a quaternary ammonium compound used in the treatment of coccidiosis. In this paper, a method based on CZE to analyze residues of Amprolium in eggs was developed and validated for the first time. Parameters such as electrolyte type, concentration, and pH were optimized. In order to improve sensitivity, field-amplified sample injection (FASI) was used for in-line preconcentration after a quick and simple sample treatment based on SPE (Envi-Carb). During method-validation studies using egg samples, a matrix interference was found at the migration time of Amprolium. This compound was identified as thiamine and confirmed by MS(n) experiments using CE coupled to MS (CE-MS) with an ion-trap mass analyzer. CZE conditions were reoptimized to separate thiamine from Amprolium allowing the quantification of Amprolium in eggs at concentrations down to 75 μg/kg, which are far below the MRL-legislated values.
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Field amplified sample injection‐capillary zone electrophoresis for the analysis of Amprolium in eggs
Electrophoresis, 2013Co-Authors: Anna Martínez-villalba, Oscar Núñez, Encarnación Moyano, M. Teresa GalceranAbstract:Veterinary medicines are widely administered to farm animals since they keep animals healthy at overcrowded conditions. Nevertheless the continuous administration of medicines to farm animals can frequently lead to the presence of residues of veterinary drugs in consumption products. Amprolium is a quaternary ammonium compound used in the treatment of coccidiosis. In this paper, a method based on CZE to analyze residues of Amprolium in eggs was developed and validated for the first time. Parameters such as electrolyte type, concentration, and pH were optimized. In order to improve sensitivity, field-amplified sample injection (FASI) was used for in-line preconcentration after a quick and simple sample treatment based on SPE (Envi-Carb). During method-validation studies using egg samples, a matrix interference was found at the migration time of Amprolium. This compound was identified as thiamine and confirmed by MS(n) experiments using CE coupled to MS (CE-MS) with an ion-trap mass analyzer. CZE conditions were reoptimized to separate thiamine from Amprolium allowing the quantification of Amprolium in eggs at concentrations down to 75 μg/kg, which are far below the MRL-legislated values.
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analysis of Amprolium by hydrophilic interaction liquid chromatography tandem mass spectrometry
Journal of Chromatography A, 2010Co-Authors: Anna Martinezvillalba, Encarnación Moyano, Teresa M GalceranAbstract:We present a fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of the coccidiostat Amprolium in food samples. Tandem mass spectrometry in a triple quadrupole was used for quantitative purposes, and the information from multiple-stage mass spectrometry in an ion-trap mass analyzer contributed to fragmentation studies. Hydrophilic interaction liquid chromatography (HILIC) in a Fused-Core column using isocratic elution (acetonitrile:formic acid/ammonium formate buffer pH 4, 50 mM (60:40)) successfully analyzed this compound in less than 3 min. The HILIC system was coupled to heated electrospray-MS/MS using highly selective-selected reaction monitoring (H-SRM) to improve sensitivity and selectivity for the analysis of Amprolium, after a simple sample treatment based on an "extract and shoot" strategy. Accurate mass measurements were performed to identify the interfering compound responsible for causing matrix ion enhancement in the signal of Amprolium. The addition of l-carnitine (the interfering compound) (1 microg L(-1)) to standards and sample extracts allowed the use of the external calibration method for quantitative purposes. The LC-MS/MS (H-SRM) method showed good precision (relative standard deviation, RSD, lower than 13%), accuracy and linearity and allowed the determination of Amprolium down to the ppb level (LODs between 0.1 and 0.6 microg kg(-1)).
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Analysis of Amprolium by hydrophilic interaction liquid chromatography–tandem mass spectrometry
Journal of chromatography. A, 2010Co-Authors: Anna Martínez-villalba, Encarnación Moyano, M. Teresa GalceranAbstract:We present a fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of the coccidiostat Amprolium in food samples. Tandem mass spectrometry in a triple quadrupole was used for quantitative purposes, and the information from multiple-stage mass spectrometry in an ion-trap mass analyzer contributed to fragmentation studies. Hydrophilic interaction liquid chromatography (HILIC) in a Fused-Core column using isocratic elution (acetonitrile:formic acid/ammonium formate buffer pH 4, 50 mM (60:40)) successfully analyzed this compound in less than 3 min. The HILIC system was coupled to heated electrospray-MS/MS using highly selective-selected reaction monitoring (H-SRM) to improve sensitivity and selectivity for the analysis of Amprolium, after a simple sample treatment based on an "extract and shoot" strategy. Accurate mass measurements were performed to identify the interfering compound responsible for causing matrix ion enhancement in the signal of Amprolium. The addition of l-carnitine (the interfering compound) (1 microg L(-1)) to standards and sample extracts allowed the use of the external calibration method for quantitative purposes. The LC-MS/MS (H-SRM) method showed good precision (relative standard deviation, RSD, lower than 13%), accuracy and linearity and allowed the determination of Amprolium down to the ppb level (LODs between 0.1 and 0.6 microg kg(-1)).
Javad Javanbakht - One of the best experts on this subject based on the ideXlab platform.
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American Journal of Internal Medicine
2016Co-Authors: Reza Sedaghat, Javad JavanbakhtAbstract:Neurohistopathological findings of the effects of Amprolium on the brain and spinal cord changes in the a animal model: An experimental stud
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Neurohistopathological findings of the effects of Amprolium on the brain and spinal cord changes in the a animal model: An experimental study
American Journal of Internal Medicine, 2014Co-Authors: Reza Sedaghat, Javad JavanbakhtAbstract:The aim of the study was to evaluate the polioencephalomalacia induced with Amprolium in goats: pathologic changes of the central nervous system. Eight apparently healthy goats of 6 to 9 weeks of age were drenched with Amprolium (200 mg/kg body weight) till the development of clinical signs. Three goats of the same age group were drenched with tap water only and these served as controls. Amprolium drenched goats were allowed to die after the onset of clinical signs and control goats were euthanised after the death of Amprolium fed goats. At the time of development of typical clinical signs of cerebrocortical necrosis (CCN) the goats were killed and necropsied and a complete histopathologic examination was performed. At necropsy of the four goats, large necrotic lesion was found in the cerebral cortex, and tissue thiamine levels decreased significantly, especially in cerebrum and cerebellum. However, the brain as a whole in all experimental animals, exhibited moderate to severe congestion, oedema and numerous small yellowish foci of varying sizes scattered on the surface of the cerebral hemispheres.Microscopic changes in the brain were limited to gray matter structures of cerebral and cerebellar cortex, caudal colliculi of mid brain, thalamus, cerebellum and spinal cord. There was shrinkage of neurons, perivascular and pericellular edema, necrosis of neurons, satellitosis, glial nodule, gliosis, middle laminar necrosis and deep laminar edema. Blood vessel walls were thickened due to hypertrophy and hyperplasia of endothelial and adventitial cells. Swollen and prominent capillary epithelium, satellitosis, presence of ghost cells, gliosis and perivascular cuffing were also observed but only in some of the animals. In the cerebellar cortex, there was degeneration of Purkinje cells. The caudal colliculi of mid brain showed bilateral malacia. To the best of our knowledge, these researchers questioned the hypothesis that the Amprolium could be the major factor causing polioencephalomalacia. This is the first documentation of Amprolium-Induced cerebrocortical necrosis on goat in Iran.
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Neurohistopathological Findings of the Effects of Amprolium on the Brain and Spinal Cord Changes in the a Animal Model
Journal of Clinical & Experimental Pathology, 2014Co-Authors: Reza Sedaghat, Javad JavanbakhtAbstract:The aim of the study was to evaluate the polioencephalomalacia induced with Amprolium in goats: pathologic changes of the central nervous system. Eight apparently healthy goats of 6 to 9 weeks of age were drenched with Amprolium (200 mg/kg body weight) till the development of clinical signs. Three goats of the same age group were drenched with tap water only and these served as controls. Amprolium drenched goats were allowed to die after the onset of clinical signs and control goats were euthanised after the death of Amprolium fed goats. At the time of development of typical clinical signs of Cerebrocortical Necrosis (CCN) the goats were killed and necropsied and a complete histopathologic examination was performed. At necropsy of the four goats, large necrotic lesion was found in the cerebral cortex, and tissue thiamine levels decreased significantly, especially in cerebrum and cerebellum. However, the brain as a whole in all experimental animals, exhibited moderate to severe congestion, oedema and numerous small yellowish foci of varying sizes scattered on the surface of the cerebral hemispheres. Microscopic changes in the brain were limited to gray matter structures of cerebral and cerebellar cortex, caudal colliculi of mid brain, thalamus, cerebellum and spinal cord. There was shrinkage of neurons, perivascular and pericellular edema, necrosis of neurons, satellitosis, glial nodule, gliosis, middle laminar necrosis and deep laminar edema. Blood vessel walls were thickened due to hypertrophy and hyperplasia of endothelial and adventitial cells. Swollen and prominent capillary epithelium, satellitosis, presence of ghost cells, gliosis and perivascular cuffing were also observed but only in some of the animals. In the cerebellar cortex, there was degeneration of Purkinje cells. The caudal colliculi of mid brain showed bilateral malacia. To the best of our knowledge, these researchers questioned the hypothesis that the Amprolium could be the major factor causing polioencephalomalacia. This is the first documentation of Amprolium-Induced cerebrocortical necrosis on goat in Iran.