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Jane R. Shaw - One of the best experts on this subject based on the ideXlab platform.

  • comparison of Veterinarian and standardized client perceptions of communication during euthanasia discussions
    Javma-journal of The American Veterinary Medical Association, 2019
    Co-Authors: Leandra Nogueira J Borden, Brenda N. Bonnett, Cindy L Adams, Carl S Ribble, Jane R. Shaw
    Abstract:

    OBJECTIVE To describe client and Veterinarian perceptions of client-centeredness during euthanasia discussions and assess agreement between measures of these perceptions. DESIGN Descriptive study. SAMPLE Stratified random sample of 32 companion animal Veterinarians in southern Ontario. PROCEDURES 2 case scenarios (a geriatric dog with worsening arthritis and a cat with inappropriate urination) designed to initiate euthanasia discussions were presented by 2 different undisclosed standardized clients (USCs) to study Veterinarian communication during clinical visits. At the end of appointments, the USC's identity was disclosed, and questionnaires to measure Veterinarian and client perceptions of client-centeredness were completed. Agreement was assessed by statistical methods. RESULTS Data were analyzed from 60 appointments (30/scenario). Of 10 questions, significant agreement was found between Veterinarians and USCs for only 1 (extent to which relevant personal and family issues were discussed; κ = 0.43) for the dog scenario and 3 (extent of discussion of respective roles [κ = 0.43], better preparedness of the USC to make a euthanasia decision [κ = 0.42], and discussion of relevant personal and family issues [κ = 0.25]) for the cat scenario. When the USC and Veterinarian disagreed, the Veterinarian perceived that the client-centeredness components were addressed more thoroughly than did the USC. CONCLUSIONS AND CLINICAL RELEVANCE Lack of agreement was found between USC and Veterinarian perceptions, with USCs perceiving less client-centeredness in euthanasia discussions. This communication gap suggested the need for training of Veterinarians in eliciting client perspectives and assessing lifestyle-social information, including client social support systems.

  • outcomes assessment of on site communication skills education in a companion animal practice
    Javma-journal of The American Veterinary Medical Association, 2016
    Co-Authors: Jane R. Shaw, Gwyn E Barley, Ashley E Hill, Kirsti Broadfoot, Debra L. Roter
    Abstract:

    OBJECTIVE To evaluate Veterinarian-client communication and Veterinarian and client satisfaction with veterinary visits before and after Veterinarians underwent a 6-month communication skills training program in a practice setting. DESIGN Case-based pretest-posttest intervention study. SAMPLE 1 purposely selected companion-animal practice. PROCEDURES The practice team (3 Veterinarians, 5 veterinary technicians, 1 receptionist, and 1 office manager) participated in a 6-month educational program (intervention) that included interactive communication modules, individual coaching, and a communication laboratory. For each of the Veterinarians, 6 appointments were video recorded and 30 additional clients completed a visit satisfaction survey both before and after the intervention. The Roter interaction analysis system was used to analyze the video-recorded appointments. RESULTS After the intervention, appointments were 5.4 minutes longer and Veterinarians asked 60% fewer closed-ended lifestyle-social questions,...

  • Gender differences in Veterinarian-client-patient communication in companion animal practice.
    Journal of the American Veterinary Medical Association, 2012
    Co-Authors: Jane R. Shaw, Brenda N. Bonnett, Cindy L Adams, Debra L. Roter, Susan Larson
    Abstract:

    Objective—To describe the relationship between Veterinarian and client genders and Veterinarian-client-patient communication. Design—Cross-sectional descriptive study. Sample—Random sample of 50 companion animal practitioners in southern Ontario and a convenience sample of 300 clients and their pets. Procedures—For each practitioner, 6 clinical appointments were videotaped, and the resulting 300 videotapes were analyzed with the Roter interaction analysis system (RIAS). Linear regression was conducted to study the relationship between demographic factors, measures of Veterinarian-client-patient communication, and gender of the Veterinarian and client. Results—Female Veterinarians conducted more relationship-centered appointments, provided more positive and rapport-building statements, talked more to the patient, and were perceived as less hurried or rushed, compared with male Veterinarians. Clients were more likely to provide lifestyle-social information to female Veterinarians. Same-gender Veterinarian-c...

  • effect of Veterinarian client patient interactions on client adherence to dentistry and surgery recommendations in companion animal practice
    Javma-journal of The American Veterinary Medical Association, 2012
    Co-Authors: Noureen Kanji, Jason B. Coe, Cindy L Adams, Jane R. Shaw
    Abstract:

    Objective—To explore the relationship between Veterinarian-client-patient interactions and client adherence to dental and surgery recommendations and to test the a priori hypotheses that appointment-specific client satisfaction and relationship-centered care are positively associated with client adherence. Design—Cross-sectional study. Sample—A subsample of 19 companion-animal Veterinarians and 83 clients from a larger observational study consisting of 20 randomly recruited Veterinarians and a convenience sample of 350 clients from eastern Ontario. Procedures—Videotaped Veterinarian-client-patient interactions containing a dentistry recommendation, surgery recommendation, or both were selected for inclusion from the larger sample of interactions coded with the Roter interaction analysis system. Client adherence was measured by evaluating each patient's medical record approximately 6 months after the videotaped interaction. The clarity of the recommendation, appointment-specific client-satisfaction score, ...

  • use of the measure of patient centered communication to analyze euthanasia discussions in companion animal practice
    Javma-journal of The American Veterinary Medical Association, 2010
    Co-Authors: Leandra Nogueira J Borden, Brenda N. Bonnett, Jane R. Shaw, Cindy L Adams, Carl S Ribble
    Abstract:

    Objective—To characterize Veterinarian-client communication with undisclosed standardized clients (USCs) during discussions regarding euthanasia of a pet. Design—Descriptive study. Sample Population—32 companion animal Veterinarians (16 males and 16 females) in southern Ontario. Procedures—During 2 clinic visits, 2 cases (a geriatric dog with worsening arthritis and a cat with inappropriate urination) designed to stimulate discussion regarding euthanasia of a pet were presented by different USCs (individuals trained to consistently present a particular case to Veterinarians without disclosing their identity). Discussions were audio recorded and analyzed by use of the measure of patient-centered communication (MPCC [a tool to assess and score physician communication behaviors]). Veterinarian and client statements were classified by means of 3 patient-centered components: exploring both the disease and the illness experience, understanding the whole person, and finding common ground. Results—60 usable recor...

Debra L. Roter - One of the best experts on this subject based on the ideXlab platform.

  • outcomes assessment of on site communication skills education in a companion animal practice
    Javma-journal of The American Veterinary Medical Association, 2016
    Co-Authors: Jane R. Shaw, Gwyn E Barley, Ashley E Hill, Kirsti Broadfoot, Debra L. Roter
    Abstract:

    OBJECTIVE To evaluate Veterinarian-client communication and Veterinarian and client satisfaction with veterinary visits before and after Veterinarians underwent a 6-month communication skills training program in a practice setting. DESIGN Case-based pretest-posttest intervention study. SAMPLE 1 purposely selected companion-animal practice. PROCEDURES The practice team (3 Veterinarians, 5 veterinary technicians, 1 receptionist, and 1 office manager) participated in a 6-month educational program (intervention) that included interactive communication modules, individual coaching, and a communication laboratory. For each of the Veterinarians, 6 appointments were video recorded and 30 additional clients completed a visit satisfaction survey both before and after the intervention. The Roter interaction analysis system was used to analyze the video-recorded appointments. RESULTS After the intervention, appointments were 5.4 minutes longer and Veterinarians asked 60% fewer closed-ended lifestyle-social questions,...

  • Gender differences in Veterinarian-client-patient communication in companion animal practice.
    Journal of the American Veterinary Medical Association, 2012
    Co-Authors: Jane R. Shaw, Brenda N. Bonnett, Cindy L Adams, Debra L. Roter, Susan Larson
    Abstract:

    Objective—To describe the relationship between Veterinarian and client genders and Veterinarian-client-patient communication. Design—Cross-sectional descriptive study. Sample—Random sample of 50 companion animal practitioners in southern Ontario and a convenience sample of 300 clients and their pets. Procedures—For each practitioner, 6 clinical appointments were videotaped, and the resulting 300 videotapes were analyzed with the Roter interaction analysis system (RIAS). Linear regression was conducted to study the relationship between demographic factors, measures of Veterinarian-client-patient communication, and gender of the Veterinarian and client. Results—Female Veterinarians conducted more relationship-centered appointments, provided more positive and rapport-building statements, talked more to the patient, and were perceived as less hurried or rushed, compared with male Veterinarians. Clients were more likely to provide lifestyle-social information to female Veterinarians. Same-gender Veterinarian-c...

  • communication skills education onsite in a veterinary practice
    Patient Education and Counseling, 2010
    Co-Authors: Jane R. Shaw, Susan Larson, Gwyn E Barley, Ashley E Hill, Debra L. Roter
    Abstract:

    Abstract Objective To provide communication skills education to veterinary professionals in the practice setting and evaluate the training by measuring Veterinarian communication pre- and post-intervention. Methods This is a case-based pre-test/post-test intervention study of a veterinary practice in Denver, CO. Four Veterinarians from a single practice and 48 clients (selected to represent wellness and problem visits) were recruited to the study. The Veterinarians took part in a training intervention consisting of a year-long curriculum, including interactive communication modules, individual coaching and communication laboratories. Six visit interactions were measured for each of the 4 Veterinarians pre- and post-skill training. The Roter interaction analysis system (RIAS) was used to analyze the study's 48 videotapes. Results Compared to the pre-training visits, Veterinarians gathered twice as much lifestyle-social data (p  Conclusion The training intervention promoted a more client-centered approach to Veterinarian–client communication. Practice implications Practice-based communication training is novel to veterinary practice. As a case study, generalization of the findings are limited, however the findings support the efficacy of the communication intervention and enhanced utilization of Veterinarian–client communication skills by these Veterinarians.

Cindy L Adams - One of the best experts on this subject based on the ideXlab platform.

  • comparison of Veterinarian and standardized client perceptions of communication during euthanasia discussions
    Javma-journal of The American Veterinary Medical Association, 2019
    Co-Authors: Leandra Nogueira J Borden, Brenda N. Bonnett, Cindy L Adams, Carl S Ribble, Jane R. Shaw
    Abstract:

    OBJECTIVE To describe client and Veterinarian perceptions of client-centeredness during euthanasia discussions and assess agreement between measures of these perceptions. DESIGN Descriptive study. SAMPLE Stratified random sample of 32 companion animal Veterinarians in southern Ontario. PROCEDURES 2 case scenarios (a geriatric dog with worsening arthritis and a cat with inappropriate urination) designed to initiate euthanasia discussions were presented by 2 different undisclosed standardized clients (USCs) to study Veterinarian communication during clinical visits. At the end of appointments, the USC's identity was disclosed, and questionnaires to measure Veterinarian and client perceptions of client-centeredness were completed. Agreement was assessed by statistical methods. RESULTS Data were analyzed from 60 appointments (30/scenario). Of 10 questions, significant agreement was found between Veterinarians and USCs for only 1 (extent to which relevant personal and family issues were discussed; κ = 0.43) for the dog scenario and 3 (extent of discussion of respective roles [κ = 0.43], better preparedness of the USC to make a euthanasia decision [κ = 0.42], and discussion of relevant personal and family issues [κ = 0.25]) for the cat scenario. When the USC and Veterinarian disagreed, the Veterinarian perceived that the client-centeredness components were addressed more thoroughly than did the USC. CONCLUSIONS AND CLINICAL RELEVANCE Lack of agreement was found between USC and Veterinarian perceptions, with USCs perceiving less client-centeredness in euthanasia discussions. This communication gap suggested the need for training of Veterinarians in eliciting client perspectives and assessing lifestyle-social information, including client social support systems.

  • clinical communication patterns of veterinary practitioners during dairy herd health and production management farm visits
    Journal of Dairy Science, 2018
    Co-Authors: Caroline Ritter, Cindy L Adams, D F Kelton, H W Barkema
    Abstract:

    Effective communication with clients is an important skill for veterinary practitioners and is linked to clients' satisfaction with the consultancy and adherence to medical advice. Detailed description of veterinary communication styles in companion animal contexts has added to the communication knowledge base and informed veterinary curricula and postgraduate education programs. The objective here was to describe veterinary practitioners' communication patterns during dairy herd health and production management farm visits. Fourteen Veterinarians in Alberta and Ontario, Canada, were provided with action cameras (Hero3 black edition; GoPro Inc., San Mateo, CA) and recorded 3 to 7 farm visits each. The resulting 70 audio-video recordings were analyzed using the Roter Interaction Analysis System. Additionally, demographic variables were obtained from study participants to investigate associations of these variables with communication patterns. Intraclass correlation coefficients were calculated for communication units, and multilevel negative binomial regression was used to compare communication patterns between demographic groups. Additionally, the relationship-centeredness of interactions was evaluated and compared between groups using linear regression models. Communication patterns varied considerably among recordings of the same Veterinarian interacting with different clients. However, most veterinary talk focused on farmer education, counseling, and building a relationship, whereas especially open-ended questions were rare. When discussion revolved around health issues of an individual animal, Veterinarians used less social talk but focused more on biomedical information gathering. Veterinarians' age and sex and the length of the Veterinarian-farmer relationship had limited association with communications; however, if the farmer and Veterinarian were both male, the conversation was more relationship centered. Communication of Veterinarians with previous communication training was very similar to that of those without training. Detailed description of veterinary practitioners' communication patterns on dairy farms will contribute to establishing the importance of communication as a clinical skill and is critical to identifying unique aspects of veterinary on-farm communication.

  • Gender differences in Veterinarian-client-patient communication in companion animal practice.
    Journal of the American Veterinary Medical Association, 2012
    Co-Authors: Jane R. Shaw, Brenda N. Bonnett, Cindy L Adams, Debra L. Roter, Susan Larson
    Abstract:

    Objective—To describe the relationship between Veterinarian and client genders and Veterinarian-client-patient communication. Design—Cross-sectional descriptive study. Sample—Random sample of 50 companion animal practitioners in southern Ontario and a convenience sample of 300 clients and their pets. Procedures—For each practitioner, 6 clinical appointments were videotaped, and the resulting 300 videotapes were analyzed with the Roter interaction analysis system (RIAS). Linear regression was conducted to study the relationship between demographic factors, measures of Veterinarian-client-patient communication, and gender of the Veterinarian and client. Results—Female Veterinarians conducted more relationship-centered appointments, provided more positive and rapport-building statements, talked more to the patient, and were perceived as less hurried or rushed, compared with male Veterinarians. Clients were more likely to provide lifestyle-social information to female Veterinarians. Same-gender Veterinarian-c...

  • effect of Veterinarian client patient interactions on client adherence to dentistry and surgery recommendations in companion animal practice
    Javma-journal of The American Veterinary Medical Association, 2012
    Co-Authors: Noureen Kanji, Jason B. Coe, Cindy L Adams, Jane R. Shaw
    Abstract:

    Objective—To explore the relationship between Veterinarian-client-patient interactions and client adherence to dental and surgery recommendations and to test the a priori hypotheses that appointment-specific client satisfaction and relationship-centered care are positively associated with client adherence. Design—Cross-sectional study. Sample—A subsample of 19 companion-animal Veterinarians and 83 clients from a larger observational study consisting of 20 randomly recruited Veterinarians and a convenience sample of 350 clients from eastern Ontario. Procedures—Videotaped Veterinarian-client-patient interactions containing a dentistry recommendation, surgery recommendation, or both were selected for inclusion from the larger sample of interactions coded with the Roter interaction analysis system. Client adherence was measured by evaluating each patient's medical record approximately 6 months after the videotaped interaction. The clarity of the recommendation, appointment-specific client-satisfaction score, ...

  • use of the measure of patient centered communication to analyze euthanasia discussions in companion animal practice
    Javma-journal of The American Veterinary Medical Association, 2010
    Co-Authors: Leandra Nogueira J Borden, Brenda N. Bonnett, Jane R. Shaw, Cindy L Adams, Carl S Ribble
    Abstract:

    Objective—To characterize Veterinarian-client communication with undisclosed standardized clients (USCs) during discussions regarding euthanasia of a pet. Design—Descriptive study. Sample Population—32 companion animal Veterinarians (16 males and 16 females) in southern Ontario. Procedures—During 2 clinic visits, 2 cases (a geriatric dog with worsening arthritis and a cat with inappropriate urination) designed to stimulate discussion regarding euthanasia of a pet were presented by different USCs (individuals trained to consistently present a particular case to Veterinarians without disclosing their identity). Discussions were audio recorded and analyzed by use of the measure of patient-centered communication (MPCC [a tool to assess and score physician communication behaviors]). Veterinarian and client statements were classified by means of 3 patient-centered components: exploring both the disease and the illness experience, understanding the whole person, and finding common ground. Results—60 usable recor...

Brusa Victoria - One of the best experts on this subject based on the ideXlab platform.

  • Comprehensive evaluation and implementation of improvement actions in bovine abattoirs to reduce pathogens exposure
    'Elsevier BV', 2020
    Co-Authors: Costa Magdalena, Brusa Victoria, Galli Lucía, Sucari Adriana, Pracca Gabriela, Ibargoyen José, Gentiluomo Jimena, Martinez Zugazua Matías, Figueroa Yamila, Londero Alejandra
    Abstract:

    The slaughter process plays an important role in animal welfare, meat quality, safety and public health through the meat production chain. In this study, we performed a three-stage evaluation: I) comprehensive evaluation, II) implementation of improvement actions and III) verification of the success of the actions implemented in three abattoirs from Argentina during 2016-2018. Risk was estimated using two checklists, quantified on a 1–100 scale and classified as high (1–40), moderate (41–70) and low (71–100). In stages I and III, Salmonella spp., E. coli O157:H7 and non-O157 STEC were detected and isolated in samples from carcasses (n = 252), the environment (n = 252); head meat (n = 21) and viscera washing and chilling water (n = 105). Carcass samples were analyzed for mesophilic aerobic organisms, coliforms and E. coli enumeration. Of 201 water samples taken, 42.0–75.6 % were non-potable quality. After the implementation of improvement actions in stage II (building, processes, systems for water purification and training), the estimation of risk of contamination was reduced from high to moderate in all three abattoirs, the count of indicator microorganisms decreased in two abattoirs, and the presence of pathogens significantly decreased. Salmonella spp. was not isolated from any of the samples collected in two abattoirs. Isolation of E. coli O157:H7 decreased in carcass and was not isolated from viscera washing and chilling water. Isolation of non-O157 STEC decreased in carcass but not in environmental samples. Finally, 75.0–95.0 % of water samples were of potable quality. Although this was only the first step in the process of change and improvement of abattoirs, the assessment of the situation and the proposal of solutions to correct deviations in a joint effort with the health authorities helped to implement a work model for enhancing food safety before meat reaches consumers.EEA RafaelaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Pracca, Gabriela. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección Provincial de Carnes; ArgentinaFil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ibargoyen, José. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección de Leche, Productos Lácteos y Derivados; ArgentinaFil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.Fil: Martinez Zugazua, Matías. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección Provincial de Carnes; ArgentinaFil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaFil: Silva, Hernán. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección Provincial de Carnes; ArgentinaFil: Ploeg, Claudia A. Van Der. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Characterization and subtyping of Listeria monocytogenes strains from butcher shops
    'Elsevier BV', 2019
    Co-Authors: Londero Alejandra, Brusa Victoria, Galli Lucía, Costa Magdalena, Linares Luciano, Prieto, Mónica Raquel, Leotta, Gerardo Aníbal
    Abstract:

    We characterized L. monocytogenes strains isolated from ground beef (n = 40) and butcher shop environmental (n = 99) samples before and after implementing improvement actions. Strains were serotyped and subtyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ApaI-pulsed-field gel electrophoresis (PFGE) to establish their epidemiological relationships, clarify the contamination dynamics and assess the impact of improvement measures. Serotype distribution was as follows: 1/2c (57.6%), 1/2b (18.7%), 4b (12.9%), 1/2a (6.5%) and 4a/4c (4.3%). Thirteen clones were simultaneously isolated in different butcher shops; 73.5% of establishments shared the same beef supplier. Cross-contamination was detected in L. monocytogenes-positive samples from 20% of butcher shops, mostly at the evaluation stage (91.7%). Up to five strains were isolated from each butcher shop at the evaluation (37%) and verification (22%) stages. Seven persistent clones were isolated over the three-year study period. Serotypes 4b and 1/2b significantly decreased during the evaluation stage. Although part of the contamination with L. monocytogenes was introduced by the beef supplier, the pathogen spread profusely in butcher shops, suggesting the relevance of keeping good hygiene control and management of the environment to prevent the spread of L. monocytogenes in butcher shops.Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Linares, Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Prieto, Mónica Raquel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Evaluation of intervention measures at different stages of the production chain in Argentinian exporting abattoirs
    'SAGE Publications', 2019
    Co-Authors: Brusa Victoria, Galli Lucía, Signorini Porchietto, Marcelo Lisandro, Restovich Viviana, Pugin Daniela, Ruíz Díaz Vanesa, Arias, Romina Raquel, Leotta, Gerardo Aníbal
    Abstract:

    Antimicrobial treatments could help to decrease the transmission of microorganisms to beef carcasses and abattoir environments. The aim of this study was to evaluate the effectiveness of interventions in reducing Shiga toxin genes (stx 1 and stx 2 ) presence in a commercial abattoir. Intervention measures included the application of electrolytically generated hypochlorous acid to steer pens (experiment 1), chlorinated water, electrolytically generated hypochlorous acid, and isoclor to steer pens (experiment 2), electrolytically generated hypochlorous acid to knocking pens (experiment 3), and aqueous ozone and electrolytically generated hypochlorous acid onto beef carcasses (experiment 4). Detection of stx in samples was performed with BAX® System Real-Time PCR Assay. Our results showed that treatment with pressurized electrolytically generated hypochlorous acid and isoclor were effective to reduce stx presence from hides on steer pens. Although there is no single strategy to ensure the reduction of stx presence in a commercial abattoir, the combined application of several antimicrobial interventions would be ideal.Fil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Restovich, Viviana. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Pugin, Daniela. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ruíz Díaz, Vanesa. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Arias, Romina Raquel. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Evaluation of intervention measures at different stages of the production chain in Argentinian exporting abattoirs
    'SAGE Publications', 2019
    Co-Authors: Brusa Victoria, Galli Lucía, Signorini Marcelo, Restovich Viviana, Pugin Daniela, Ruíz Díaz Vanesa, Arias Romina, Leotta, Gerardo Aníbal
    Abstract:

    Antimicrobial treatments could help to decrease the transmission of microorganisms to beef carcasses and abattoir environments. The aim of this study was to evaluate the effectiveness of interventions in reducing Shiga toxin genes (stx1 and stx2) presence in a commercial abattoir. Intervention measures included the application of electrolytically generated hypochlorous acid to steer pens (experiment 1), chlorinated water, electrolytically generated hypochlorous acid, and isoclor to steer pens (experiment 2), electrolytically generated hypochlorous acid to knocking pens (experiment 3), and aqueous ozone and electrolytically generated hypochlorous acid onto beef carcasses (experiment 4). Detection of stx in samples was performed with BAX® System Real-Time PCR Assay. Our results showed that treatment with pressurized electrolytically generated hypochlorous acid and isoclor were effective to reduce stx presence from hides on steer pens. Although there is no single strategy to ensure the reduction of stx presence in a commercial abattoir, the combined application of several antimicrobial interventions would be ideal.EEA RafaelaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.Fil: Restovich, Viviana. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pugin, Daniela. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ruíz Díaz, Vanesa. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Arias, Romina. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina
    'Public Library of Science (PLoS)', 2017
    Co-Authors: Brusa Victoria, Galli Lucía, Signorini Marcelo, Londero Alejandra, Restovich Viviana, Teitelbaum David, Brasesco Hebe, García Diego, Padola, Nora Lía, Superno Valeria
    Abstract:

    Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965) from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193) from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%), 111 pools of cuts (5.8%) and 45 pools of trimmings (7.0%) were positive for non-O157 STEC. STEC strains (n = 200) were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b) and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a stringent monitoring system. Likewise, zero-tolerance intervention measures should be applied in beef, together with GMP and HACCP. Further, collaborative efforts for risk assessment, management and communication are extremely important to improve the safety of foodstuffs.EEA RafaelaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Microbiología de Alimentos; ArgentinaFil: Restovich, Viviana. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Teitelbaum, David. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brasesco, Hebe. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: García, Diego. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Superno, Valeria. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Sanz, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Petroli, Sandra. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Bruzzone, Mariana. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Alimentos; ArgentinaFil: Ferreghini, Marcela. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Linares, Luciano. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Microbiología de Alimentos; ArgentinaFil: Suberbie, Germán. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Rodriguez, Hector Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Economía; ArgentinaFil: Leotta, Gerardo A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

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  • Comprehensive evaluation and implementation of improvement actions in bovine abattoirs to reduce pathogens exposure
    'Elsevier BV', 2020
    Co-Authors: Costa Magdalena, Brusa Victoria, Galli Lucía, Sucari Adriana, Pracca Gabriela, Ibargoyen José, Gentiluomo Jimena, Martinez Zugazua Matías, Figueroa Yamila, Londero Alejandra
    Abstract:

    The slaughter process plays an important role in animal welfare, meat quality, safety and public health through the meat production chain. In this study, we performed a three-stage evaluation: I) comprehensive evaluation, II) implementation of improvement actions and III) verification of the success of the actions implemented in three abattoirs from Argentina during 2016-2018. Risk was estimated using two checklists, quantified on a 1–100 scale and classified as high (1–40), moderate (41–70) and low (71–100). In stages I and III, Salmonella spp., E. coli O157:H7 and non-O157 STEC were detected and isolated in samples from carcasses (n = 252), the environment (n = 252); head meat (n = 21) and viscera washing and chilling water (n = 105). Carcass samples were analyzed for mesophilic aerobic organisms, coliforms and E. coli enumeration. Of 201 water samples taken, 42.0–75.6 % were non-potable quality. After the implementation of improvement actions in stage II (building, processes, systems for water purification and training), the estimation of risk of contamination was reduced from high to moderate in all three abattoirs, the count of indicator microorganisms decreased in two abattoirs, and the presence of pathogens significantly decreased. Salmonella spp. was not isolated from any of the samples collected in two abattoirs. Isolation of E. coli O157:H7 decreased in carcass and was not isolated from viscera washing and chilling water. Isolation of non-O157 STEC decreased in carcass but not in environmental samples. Finally, 75.0–95.0 % of water samples were of potable quality. Although this was only the first step in the process of change and improvement of abattoirs, the assessment of the situation and the proposal of solutions to correct deviations in a joint effort with the health authorities helped to implement a work model for enhancing food safety before meat reaches consumers.EEA RafaelaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Pracca, Gabriela. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección Provincial de Carnes; ArgentinaFil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ibargoyen, José. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección de Leche, Productos Lácteos y Derivados; ArgentinaFil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.Fil: Martinez Zugazua, Matías. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección Provincial de Carnes; ArgentinaFil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaFil: Silva, Hernán. Ministerio de Agroindustria de la Provincia de Buenos Aires. Dirección Provincial de Carnes; ArgentinaFil: Ploeg, Claudia A. Van Der. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Characterization and subtyping of Listeria monocytogenes strains from butcher shops
    'Elsevier BV', 2019
    Co-Authors: Londero Alejandra, Brusa Victoria, Galli Lucía, Costa Magdalena, Linares Luciano, Prieto, Mónica Raquel, Leotta, Gerardo Aníbal
    Abstract:

    We characterized L. monocytogenes strains isolated from ground beef (n = 40) and butcher shop environmental (n = 99) samples before and after implementing improvement actions. Strains were serotyped and subtyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ApaI-pulsed-field gel electrophoresis (PFGE) to establish their epidemiological relationships, clarify the contamination dynamics and assess the impact of improvement measures. Serotype distribution was as follows: 1/2c (57.6%), 1/2b (18.7%), 4b (12.9%), 1/2a (6.5%) and 4a/4c (4.3%). Thirteen clones were simultaneously isolated in different butcher shops; 73.5% of establishments shared the same beef supplier. Cross-contamination was detected in L. monocytogenes-positive samples from 20% of butcher shops, mostly at the evaluation stage (91.7%). Up to five strains were isolated from each butcher shop at the evaluation (37%) and verification (22%) stages. Seven persistent clones were isolated over the three-year study period. Serotypes 4b and 1/2b significantly decreased during the evaluation stage. Although part of the contamination with L. monocytogenes was introduced by the beef supplier, the pathogen spread profusely in butcher shops, suggesting the relevance of keeping good hygiene control and management of the environment to prevent the spread of L. monocytogenes in butcher shops.Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Linares, Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Prieto, Mónica Raquel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Evaluation of intervention measures at different stages of the production chain in Argentinian exporting abattoirs
    'SAGE Publications', 2019
    Co-Authors: Brusa Victoria, Galli Lucía, Signorini Porchietto, Marcelo Lisandro, Restovich Viviana, Pugin Daniela, Ruíz Díaz Vanesa, Arias, Romina Raquel, Leotta, Gerardo Aníbal
    Abstract:

    Antimicrobial treatments could help to decrease the transmission of microorganisms to beef carcasses and abattoir environments. The aim of this study was to evaluate the effectiveness of interventions in reducing Shiga toxin genes (stx 1 and stx 2 ) presence in a commercial abattoir. Intervention measures included the application of electrolytically generated hypochlorous acid to steer pens (experiment 1), chlorinated water, electrolytically generated hypochlorous acid, and isoclor to steer pens (experiment 2), electrolytically generated hypochlorous acid to knocking pens (experiment 3), and aqueous ozone and electrolytically generated hypochlorous acid onto beef carcasses (experiment 4). Detection of stx in samples was performed with BAX® System Real-Time PCR Assay. Our results showed that treatment with pressurized electrolytically generated hypochlorous acid and isoclor were effective to reduce stx presence from hides on steer pens. Although there is no single strategy to ensure the reduction of stx presence in a commercial abattoir, the combined application of several antimicrobial interventions would be ideal.Fil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Restovich, Viviana. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Pugin, Daniela. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ruíz Díaz, Vanesa. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Arias, Romina Raquel. Instituto de la Promoción de la Carne Vacuna Argentina; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Evaluation of intervention measures at different stages of the production chain in Argentinian exporting abattoirs
    'SAGE Publications', 2019
    Co-Authors: Brusa Victoria, Galli Lucía, Signorini Marcelo, Restovich Viviana, Pugin Daniela, Ruíz Díaz Vanesa, Arias Romina, Leotta, Gerardo Aníbal
    Abstract:

    Antimicrobial treatments could help to decrease the transmission of microorganisms to beef carcasses and abattoir environments. The aim of this study was to evaluate the effectiveness of interventions in reducing Shiga toxin genes (stx1 and stx2) presence in a commercial abattoir. Intervention measures included the application of electrolytically generated hypochlorous acid to steer pens (experiment 1), chlorinated water, electrolytically generated hypochlorous acid, and isoclor to steer pens (experiment 2), electrolytically generated hypochlorous acid to knocking pens (experiment 3), and aqueous ozone and electrolytically generated hypochlorous acid onto beef carcasses (experiment 4). Detection of stx in samples was performed with BAX® System Real-Time PCR Assay. Our results showed that treatment with pressurized electrolytically generated hypochlorous acid and isoclor were effective to reduce stx presence from hides on steer pens. Although there is no single strategy to ensure the reduction of stx presence in a commercial abattoir, the combined application of several antimicrobial interventions would be ideal.EEA RafaelaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.Fil: Restovich, Viviana. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pugin, Daniela. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ruíz Díaz, Vanesa. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Arias, Romina. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

  • Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina
    'Public Library of Science (PLoS)', 2017
    Co-Authors: Brusa Victoria, Galli Lucía, Signorini Marcelo, Londero Alejandra, Restovich Viviana, Teitelbaum David, Brasesco Hebe, García Diego, Padola, Nora Lía, Superno Valeria
    Abstract:

    Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965) from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193) from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%), 111 pools of cuts (5.8%) and 45 pools of trimmings (7.0%) were positive for non-O157 STEC. STEC strains (n = 200) were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b) and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a stringent monitoring system. Likewise, zero-tolerance intervention measures should be applied in beef, together with GMP and HACCP. Further, collaborative efforts for risk assessment, management and communication are extremely important to improve the safety of foodstuffs.EEA RafaelaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Microbiología de Alimentos; ArgentinaFil: Restovich, Viviana. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Teitelbaum, David. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brasesco, Hebe. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: García, Diego. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Superno, Valeria. Instituto de Promoción de la Carne Vacuna Argentina (IPCVA); ArgentinaFil: Sanz, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. 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