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Sam Dyal - One of the best experts on this subject based on the ideXlab platform.

Rachel Martinez - One of the best experts on this subject based on the ideXlab platform.

B I Kurganov - One of the best experts on this subject based on the ideXlab platform.

  • editor note Biochemistry Analytical Biochemistry
    Biochemistry & Analytical Biochemistry, 2016
    Co-Authors: B I Kurganov
    Abstract:

    Biochemistry is the study of structures, functions and interactions of biomolecules, such as carbohydrates, nucleic acids, proteins, lipids, etc. and their metabolisms. Advancements in Biochemistry helps in the advancement of different fields like agriculture, medicine, nutrition, etc., which are directly related to it. Combination of biochemical techniques with techniques and ideas of genetics, molecular biology and biophysics had facilitated the discoveries and inventions in the field of Biochemistry. Analytical chemistry is focused on the creation of new measurement techniques and tools, experimental designs, chemometrics, etc., playing an important role in opening new avenues in Biochemistry. Recently the research in Biochemistry reached to molecular level and now the terms molecular biology and Biochemistry have become nearly interchangeable.

  • Editor Note - Biochemistry & Analytical Biochemistry
    Biochemistry & Analytical Biochemistry, 2016
    Co-Authors: B I Kurganov
    Abstract:

    Biochemistry is the study of structures, functions and interactions of biomolecules, such as carbohydrates, nucleic acids, proteins, lipids, etc. and their metabolisms. Advancements in Biochemistry helps in the advancement of different fields like agriculture, medicine, nutrition, etc., which are directly related to it. Combination of biochemical techniques with techniques and ideas of genetics, molecular biology and biophysics had facilitated the discoveries and inventions in the field of Biochemistry. Analytical chemistry is focused on the creation of new measurement techniques and tools, experimental designs, chemometrics, etc., playing an important role in opening new avenues in Biochemistry. Recently the research in Biochemistry reached to molecular level and now the terms molecular biology and Biochemistry have become nearly interchangeable.

  • Selection of Test Systems for Estimation of Anti-aggregation Activity of Molecular Chaperones
    Biochemistry & Analytical Biochemistry, 2015
    Co-Authors: B I Kurganov
    Abstract:

    Searching the agents that are capable of effectively suppressing protein aggregation and elaboration of the corresponding test systems are among the most important problems of modern Biochemistry and Analytical Biochemistry [1-7]. To characterize the anti-aggregation activity of different agents including chaperones of protein nature and low-molecular-weight chemical chaperones, test systems where the initial state of a target protein is the native protein that undergoes unfolding and subsequent aggregation under the action of different factors are mostly used. Unfolding of the target protein can be caused by heating at elevated temperatures. For several proteins the unfolding process can be initiated by the cleavage of S-S bonds in the protein molecule. Dithiothreitol (DTT)-induced aggregation was demonstrated, for example, for α-lactalbumin, insulin, lysozyme and bovine serum albumin (BSA).

Joel N Hirschhorn - One of the best experts on this subject based on the ideXlab platform.

Joanne Sun - One of the best experts on this subject based on the ideXlab platform.

  • Heterogeneity of monoclonal antibodies
    Journal of Pharmaceutical Sciences, 2008
    Co-Authors: Hongcheng Liu, Georgeen Caza-bulseco, Dinesh Faldu, Chris Chumsae, Joanne Sun
    Abstract:

    ABSTRACT: Heterogeneity of monoclonal antibodies is common due to the various modifications introduced over the lifespan of the molecules from the point of synthesis to the point of complete clearance from the subjects. The vast number of modifications presents great challenge to the thorough characterization of the molecules. This article reviews the current knowledge of enzymatic and nonenzymatic modifications of monoclonal antibodies including the common ones such as incomplete disulfide bond formation, glycosylation, N-terminal pyroglutamine cyclization, C-terminal lysine processing, deamidation, isomerization, and oxidation, and less common ones such as modification of the N-terminal amino acids by maleuric acid and amidation of the C-terminal amino acid. In addition, noncovalent associations with other molecules, conformational diversity and aggregation of monoclonal antibodies are also discussed. Through a complete understanding of the heterogeneity of monoclonal antibodies, strategies can be employed to better identify the potential modifications and thoroughly characterize the molecules. 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:2426–2447, 2008 Keywords: Structure; Stability; Protein aggregation; Glycosylation; Deamidation; Analytical Biochemistry