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Xiaolan Zhong - One of the best experts on this subject based on the ideXlab platform.
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transcriptome sequence analysis of an ornamental plant Ananas Comosus var bracteatus revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis
PLOS ONE, 2015Co-Authors: Surapathrudu Kanakala, Yehua He, Junli Zhang, Xiaolan ZhongAbstract:Background Ananas Comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus high-throughput transcriptome sequencing of Ananas Comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.
Andre Manaroinsong - One of the best experts on this subject based on the ideXlab platform.
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UJI DAYA HAMBAT EKSTRAK KULIT NANAS (Ananas Comosus L) TERHADAP BAKTERI Staphylococcus aureus SECARA IN VITRO
2015Co-Authors: Andre ManaroinsongAbstract:UJI DAYA HAMBAT EKSTRAK KULIT NANAS ( Ananas Comosus L) TERHADAP BAKTERI Staphylococcus aureus SECARA IN VITRO Andre Manaroinsong 1) , Jemmy Abidjulu 2) , Krista V. Siagian 1) 1 ) Program Studi Kedokteran Gigi Fakultas Kedokteran 2 ) Fakultas MIPA, UNSRAT Abstract Herbal plants in Indonesia has been mostly used as a traditional medicine. One of the natural materials is pineapple plant (Ananas Comosus L). Pineapple plants contain bromelain enzyme which is trusted as bactericidal. One of the oral health problems found is oral abcess. Abcess is a local accumulation of pus in a cavity which happens because of pyogenic bactery infection. One of the cautions of abcess is Staphylococcus aureus bactery. The purpose of this study to determine the inhibition of skin and meat of the pineapple on the growth of Staphylococcus aureus. This research is an experimental research using the post-test only control group design with modification method of Kirby-Bauer by using paper disk. Pinapple skin extract and pinapple meat can be obtained with maceration method with etanol 96% solvent. Staphylococcus aureus is taken from a pure stock of farmacy laboratorium MIPA faculty Unsrat. Results of this study addressing the pineapple meat and skin extracts have inhibitory to growth of Stapylococcus aureus. The average diameter zone of inhibition against Staphylococcus aureus pineapple skin of 15.06 mm and pineapple meat of 10.85 mm. From this study it can be concluded that the extract of pineapple skin have inhibitory bigger than meat pineapple but still less effective than the positive control Clindamycin. The results could be used as insert in order to conduct further research on the pineapple in inhibiting bacteria Staphylococcus aureus as a cause abscesses in the oral cavity. Keywords: pineapple (Ananas Comosus L), Staphylococcus aureus ABSTRAK Tanaman herbal di Indonesia telah banyak digunakan sebagai obat tradisional. Salah satu bahan alami adalah tanaman Nanas (Ananas Comosus L) . tanaman nanas memiliki kandungan enzim bromelain yang dipercaya dapat membunuh bakteri. Salah satu masalah kesehatan rongga mulut yang sering ditemukan adalah abses rongga mulut, abses adalah pengumpulan nanas secara lokal dalam suatu kavitas yang terjadi karena hancurnya jaringan, biasanya disebabkan oleh infeksi bakteri piogenik. Salah satu penyebab abses ialah dari infeksi bakteri Staphylococcus aureus. Tujuan penelitian ini untuk mengetahui daya hambat kulit dan daging nanas terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini merupakan penelitian eksperimental menggunakan desain post-test only control group design dengan menggunakan metode modifikasi Kirby-Bauer menggunakan kertas saring. Ekstrak kulit nanas didapat dengan metode maserasi dengan pelarut etanol 96%. Bakteri Stapylococcus aureus diambil dari stok murni Laboratorium farmasi Fakultas MIPA Unsrat. Hasil penelitian ini menujukan ekstrak kulit dan daging nanas memiliki daya hambat terhadap pertumbuhan bakteri Stapylococcus aureus. Rata-rata diameter zona hambat ekstrak kulit nanas terhadap Staphylococcus aureus sebesar 15,06 mm dan daging nanas sebesar 10,85 mm. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak kulit nanas memiliki daya hambat yang lebih besar daripada daging nanas tetapi masih kurang besar dibandingkan dengan kontrol positif Clindamycin . Hasil penelitian ini dapat dijadikan masukkan agar dilakukan penelitian lebih lanjut mengenai nanas dalam menghambat bakteri Staphylococcus aureus sebagai penyebab abses dalam rongga mulut. Kata kunci : tanaman nanas (Ananas Comosus L) , Staphylococcus aureus
Surapathrudu Kanakala - One of the best experts on this subject based on the ideXlab platform.
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transcriptome sequence analysis of an ornamental plant Ananas Comosus var bracteatus revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis
PLOS ONE, 2015Co-Authors: Surapathrudu Kanakala, Yehua He, Junli Zhang, Xiaolan ZhongAbstract:Background Ananas Comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus high-throughput transcriptome sequencing of Ananas Comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.
Yehua He - One of the best experts on this subject based on the ideXlab platform.
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Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas Comosus var. bracteatus
BMC Genomics, 2020Co-Authors: Yehua He, Xuzixing Zhou, Fatima Rafique, Hao Hu, Lijun Feng, Wei Yang, Xi LiAbstract:Lysine succinylation, an important protein posttranslational modification (PTM), is widespread and conservative. The regulatory functions of succinylation in leaf color has been reported. The chimeric leaves of Ananas Comosus var. bracteatus are composed of normal green parts and albino white parts. However, the extent and function of lysine succinylation in chimeric leaves of Ananas Comosus var. bracteatus has yet to be investigated. Compared to the green (Gr) parts, the global succinylation level was increased in the white (Wh) parts of chimeric leaves according to the Western blot and immunohistochemistry analysis. Furthermore, we quantitated the change in the succinylation profiles between the Wh and Gr parts of chimeric leaves using label-free LFQ intensity. In total, 855 succinylated sites in 335 proteins were identified, and 593 succinylated sites in 237 proteins were quantified. Compared to the Gr parts, 232 (61.1%) sites in 128 proteins were quantified as upregulated targets, and 148 (38.9%) sites in 70 proteins were quantified as downregulated targets in the Wh parts of chimeric leaves using a 1.5-fold threshold (P
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transcriptome sequence analysis of an ornamental plant Ananas Comosus var bracteatus revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis
PLOS ONE, 2015Co-Authors: Surapathrudu Kanakala, Yehua He, Junli Zhang, Xiaolan ZhongAbstract:Background Ananas Comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus high-throughput transcriptome sequencing of Ananas Comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.
Indah Santi Pratiwi - One of the best experts on this subject based on the ideXlab platform.
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EFEKTIVITAS EKSTRAK KULIT NANAS (Ananas Comosus) TERHADAP BAKTERI Staphylococcus aureus (Secara in vitro)
2018Co-Authors: Indah Santi PratiwiAbstract:Latar Belakang : Penyakit gigi dan mulut menduduki urutan pertama yaitu sebesar 60% di Indonesia. Salah satu bakteri penyebab penyakit rongga mulut adalah bakteri Staphylococcus aureus. Bakteri Staphylococcus aureus merupakan penyebab gingivitis yang termasuk dalam golongan bakteri gram positif, anaerob. Nanas (Ananas Comosus) adalah tanaman yang telah tersebar luas ke seluruh dunia dan dapat tumbuh di dataran rendah maupun dataran tinggi. Pada bagian kulit buah nanas (Ananas Comosus) mengandung zat-zat aktif yang memiliki daya antibakteri. Tujuan : penelitian ini bertujuan untuk menguji Kadar Hambat Minimal (KHM) dan Kadar Bunuh Minimal (KBM) ekstrak kulit nanas (Ananas Comosus) dengan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, dan 0,78% terhadap bakteri Staphylococcus aureus secara in vitro. Metode : Jenis penelitian adalah penelitian ekperimental murni laboratoris (in vitro). Uji daya antibakteri dilakukan dengan metode dilusi cair pada media Brain Heart Infusion (BHI) dan metode dilusi padat pada media Triton Soya Agar (TSA). Ekstrak kulit nanas (Ananas Comosus) diencerkan dengan cara berseri kedalam beberapa konsentrasi: 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, dan 0,78%. Kadar hambat minimal dan kadar bunuh minimal ditentukan dengan mengamati dari pertumbuhan bakteri Staphylococcus aureus media BHI dan TSA. Hasil : Kadar Hambat Minimal (KHM) terdapat pada konsentrasi 1,56% dan Kadar Bunuh minimal (KBM) terdapat pada konsentrasi 3,125%. Kesimpulan: Ekstrak kulit nanas (Ananas Comosus) efektif dalam menghambat maupun membunuh bakteri Staphylococcus aureus.
