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Oleg Mediannikov - One of the best experts on this subject based on the ideXlab platform.

  • Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France
    Parasites & vectors, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Djamel Tahir, Oleg Mediannikov
    Abstract:

    Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia. The aim of this study was to identify and estimate the prevalence of Anaplasmataceae species infecting domestic animals and ticks in Corsica. In this study, 458 blood samples from sheep, cattle, horses, goats, dogs, and 123 ticks removed from cattle, were collected in Corsica. Quantitative real-time PCR screening and genetic characterisation of Anaplasmataceae bacteria were based on the 23S rRNA, rpoB and groEl genes. Two tick species were collected in the present study: Rhipicephalus bursa (118) and Hyalomma marginatum marginatum (5). Molecular investigation showed that 32.1% (147/458) of blood samples were positive for Anaplasmataceae Infection. Anaplasma ovis was identified in 42.3% (93/220) of sheep. Anaplasma marginale was amplified from 100% (12/12) of cattle and two R. bursa (2/123). Several potentially new species were also identified: Anaplasma cf. ovis, “Candidatus Anaplasma corsicanum”, “Candidatus Anaplasma mediterraneum” were amplified from 17.3% (38/220) of sheep, and Anaplasma sp. marginale-like was amplified from 80% (4/5) of goats. Finally, one R. bursa tick was found to harbour the DNA of E. canis. All samples from horses and dogs were negative for Anaplasmataceae Infection. To our knowledge, this study is the first epidemiological survey on Anaplasmataceae species infecting animals and ticks in Corsica and contributes toward the identification of current Anaplasmataceae species circulating in Corsica.

  • natural Anaplasmataceae Infection in rhipicephalus bursa ticks collected from sheep in the french basque country
    Ticks and Tick-borne Diseases, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in ă the family of Ixodidae. In this study, we investigated Anaplasmataceae ă bacteria associated with R. bursa collected after an epizootic outbreak ă of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), ă were removed from sheep in two farms and all identified as R. bursa, all ă females were partially engorged. We found that 50% of the ticks were ă positive in the initial Anaplasmataceae qPCR screening. Bacterial ă species was identified by analyzing the sequences of amplicons of 23S ă rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma ă phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA ă and groEL genes analysis, we found that 19.7% of ticks contained a ă potentially new species of Ehrlichia. We propose the status of ă Candidatus for this uncultured species and we provisionally name it ă Candidatus Ehrlichia urmitei. No Wolbachia were identified. These ă results show that R. bursa can be a carrier of Anaplasmataceae bacteria. ă (C) 2016 Elsevier GmbH. All rights reserved.

  • Natural Anaplasmataceae Infection in Rhipicephalus bursa ticks collected from sheep in the French Basque Country.
    Ticks and tick-borne diseases, 2016
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in the family of Ixodidae. In this study, we investigated Anaplasmataceae bacteria associated with R. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as R. bursa, all females were partially engorged. We found that 50% of the ticks were positive in the initial Anaplasmataceae qPCR screening. Bacterial species was identified by analyzing the sequences of amplicons of 23S rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA and groEL genes analysis, we found that 19.7% of ticks contained a potentially new species of Ehrlichia. We propose the status of Candidatus for this uncultured species and we provisionally name it Candidatus Ehrlichia urmitei. No Wolbachia were identified. These results show that R. bursa can be a carrier of Anaplasmataceae bacteria.

Mustapha Dahmani - One of the best experts on this subject based on the ideXlab platform.

  • Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France
    Parasites & vectors, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Djamel Tahir, Oleg Mediannikov
    Abstract:

    Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia. The aim of this study was to identify and estimate the prevalence of Anaplasmataceae species infecting domestic animals and ticks in Corsica. In this study, 458 blood samples from sheep, cattle, horses, goats, dogs, and 123 ticks removed from cattle, were collected in Corsica. Quantitative real-time PCR screening and genetic characterisation of Anaplasmataceae bacteria were based on the 23S rRNA, rpoB and groEl genes. Two tick species were collected in the present study: Rhipicephalus bursa (118) and Hyalomma marginatum marginatum (5). Molecular investigation showed that 32.1% (147/458) of blood samples were positive for Anaplasmataceae Infection. Anaplasma ovis was identified in 42.3% (93/220) of sheep. Anaplasma marginale was amplified from 100% (12/12) of cattle and two R. bursa (2/123). Several potentially new species were also identified: Anaplasma cf. ovis, “Candidatus Anaplasma corsicanum”, “Candidatus Anaplasma mediterraneum” were amplified from 17.3% (38/220) of sheep, and Anaplasma sp. marginale-like was amplified from 80% (4/5) of goats. Finally, one R. bursa tick was found to harbour the DNA of E. canis. All samples from horses and dogs were negative for Anaplasmataceae Infection. To our knowledge, this study is the first epidemiological survey on Anaplasmataceae species infecting animals and ticks in Corsica and contributes toward the identification of current Anaplasmataceae species circulating in Corsica.

  • natural Anaplasmataceae Infection in rhipicephalus bursa ticks collected from sheep in the french basque country
    Ticks and Tick-borne Diseases, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in ă the family of Ixodidae. In this study, we investigated Anaplasmataceae ă bacteria associated with R. bursa collected after an epizootic outbreak ă of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), ă were removed from sheep in two farms and all identified as R. bursa, all ă females were partially engorged. We found that 50% of the ticks were ă positive in the initial Anaplasmataceae qPCR screening. Bacterial ă species was identified by analyzing the sequences of amplicons of 23S ă rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma ă phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA ă and groEL genes analysis, we found that 19.7% of ticks contained a ă potentially new species of Ehrlichia. We propose the status of ă Candidatus for this uncultured species and we provisionally name it ă Candidatus Ehrlichia urmitei. No Wolbachia were identified. These ă results show that R. bursa can be a carrier of Anaplasmataceae bacteria. ă (C) 2016 Elsevier GmbH. All rights reserved.

  • Natural Anaplasmataceae Infection in Rhipicephalus bursa ticks collected from sheep in the French Basque Country.
    Ticks and tick-borne diseases, 2016
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in the family of Ixodidae. In this study, we investigated Anaplasmataceae bacteria associated with R. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as R. bursa, all females were partially engorged. We found that 50% of the ticks were positive in the initial Anaplasmataceae qPCR screening. Bacterial species was identified by analyzing the sequences of amplicons of 23S rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA and groEL genes analysis, we found that 19.7% of ticks contained a potentially new species of Ehrlichia. We propose the status of Candidatus for this uncultured species and we provisionally name it Candidatus Ehrlichia urmitei. No Wolbachia were identified. These results show that R. bursa can be a carrier of Anaplasmataceae bacteria.

Florence Fenollar - One of the best experts on this subject based on the ideXlab platform.

  • Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France
    Parasites & vectors, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Djamel Tahir, Oleg Mediannikov
    Abstract:

    Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia. The aim of this study was to identify and estimate the prevalence of Anaplasmataceae species infecting domestic animals and ticks in Corsica. In this study, 458 blood samples from sheep, cattle, horses, goats, dogs, and 123 ticks removed from cattle, were collected in Corsica. Quantitative real-time PCR screening and genetic characterisation of Anaplasmataceae bacteria were based on the 23S rRNA, rpoB and groEl genes. Two tick species were collected in the present study: Rhipicephalus bursa (118) and Hyalomma marginatum marginatum (5). Molecular investigation showed that 32.1% (147/458) of blood samples were positive for Anaplasmataceae Infection. Anaplasma ovis was identified in 42.3% (93/220) of sheep. Anaplasma marginale was amplified from 100% (12/12) of cattle and two R. bursa (2/123). Several potentially new species were also identified: Anaplasma cf. ovis, “Candidatus Anaplasma corsicanum”, “Candidatus Anaplasma mediterraneum” were amplified from 17.3% (38/220) of sheep, and Anaplasma sp. marginale-like was amplified from 80% (4/5) of goats. Finally, one R. bursa tick was found to harbour the DNA of E. canis. All samples from horses and dogs were negative for Anaplasmataceae Infection. To our knowledge, this study is the first epidemiological survey on Anaplasmataceae species infecting animals and ticks in Corsica and contributes toward the identification of current Anaplasmataceae species circulating in Corsica.

  • natural Anaplasmataceae Infection in rhipicephalus bursa ticks collected from sheep in the french basque country
    Ticks and Tick-borne Diseases, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in ă the family of Ixodidae. In this study, we investigated Anaplasmataceae ă bacteria associated with R. bursa collected after an epizootic outbreak ă of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), ă were removed from sheep in two farms and all identified as R. bursa, all ă females were partially engorged. We found that 50% of the ticks were ă positive in the initial Anaplasmataceae qPCR screening. Bacterial ă species was identified by analyzing the sequences of amplicons of 23S ă rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma ă phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA ă and groEL genes analysis, we found that 19.7% of ticks contained a ă potentially new species of Ehrlichia. We propose the status of ă Candidatus for this uncultured species and we provisionally name it ă Candidatus Ehrlichia urmitei. No Wolbachia were identified. These ă results show that R. bursa can be a carrier of Anaplasmataceae bacteria. ă (C) 2016 Elsevier GmbH. All rights reserved.

  • Natural Anaplasmataceae Infection in Rhipicephalus bursa ticks collected from sheep in the French Basque Country.
    Ticks and tick-borne diseases, 2016
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in the family of Ixodidae. In this study, we investigated Anaplasmataceae bacteria associated with R. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as R. bursa, all females were partially engorged. We found that 50% of the ticks were positive in the initial Anaplasmataceae qPCR screening. Bacterial species was identified by analyzing the sequences of amplicons of 23S rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA and groEL genes analysis, we found that 19.7% of ticks contained a potentially new species of Ehrlichia. We propose the status of Candidatus for this uncultured species and we provisionally name it Candidatus Ehrlichia urmitei. No Wolbachia were identified. These results show that R. bursa can be a carrier of Anaplasmataceae bacteria.

Goudarz Molaei - One of the best experts on this subject based on the ideXlab platform.

Frederic Rousseau - One of the best experts on this subject based on the ideXlab platform.

  • natural Anaplasmataceae Infection in rhipicephalus bursa ticks collected from sheep in the french basque country
    Ticks and Tick-borne Diseases, 2017
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in ă the family of Ixodidae. In this study, we investigated Anaplasmataceae ă bacteria associated with R. bursa collected after an epizootic outbreak ă of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), ă were removed from sheep in two farms and all identified as R. bursa, all ă females were partially engorged. We found that 50% of the ticks were ă positive in the initial Anaplasmataceae qPCR screening. Bacterial ă species was identified by analyzing the sequences of amplicons of 23S ă rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma ă phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA ă and groEL genes analysis, we found that 19.7% of ticks contained a ă potentially new species of Ehrlichia. We propose the status of ă Candidatus for this uncultured species and we provisionally name it ă Candidatus Ehrlichia urmitei. No Wolbachia were identified. These ă results show that R. bursa can be a carrier of Anaplasmataceae bacteria. ă (C) 2016 Elsevier GmbH. All rights reserved.

  • Natural Anaplasmataceae Infection in Rhipicephalus bursa ticks collected from sheep in the French Basque Country.
    Ticks and tick-borne diseases, 2016
    Co-Authors: Mustapha Dahmani, Florence Fenollar, Frederic Rousseau, Oleg Mediannikov
    Abstract:

    Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in the family of Ixodidae. In this study, we investigated Anaplasmataceae bacteria associated with R. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as R. bursa, all females were partially engorged. We found that 50% of the ticks were positive in the initial Anaplasmataceae qPCR screening. Bacterial species was identified by analyzing the sequences of amplicons of 23S rRNA, groEL and rpoB genes. 22.4% of ticks contained DNA of Anaplasma phagocytophilum and 7.9% the DNA of Anaplasma ovis. Based on 23S rRNA and groEL genes analysis, we found that 19.7% of ticks contained a potentially new species of Ehrlichia. We propose the status of Candidatus for this uncultured species and we provisionally name it Candidatus Ehrlichia urmitei. No Wolbachia were identified. These results show that R. bursa can be a carrier of Anaplasmataceae bacteria.